id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-007187-gb1txu1o	Lindner, Juha	CD4(+) T Helper Cell Responses against Human Bocavirus Viral Protein 2 Viruslike Particles in Healthy Adults	2008-12-01		.txt	text/plain	3919	180	45	To date, HBoV genomes have been detected worldwide in respiratory tract samples obtained from children with pulmonary diseases, whereas only limited data on virus-specific immunity are available, mainly because of the lack of recombinant viral antigens. seronegative individuals have been described and were found predominantly among infants and young children (those 2 years of age) [20, 27, 28, 29] , serum samples obtained from 10 healthy infants (mean age, 11.8 months [range, 6 -45 months]) without detectable virus-specific antibodies (median OD 450 value, 0.056 [range, 0.017-0.104]) were selected and representatively included in the study, to ensure the specificity of the serologic analysis performed. By use of viral protein 2 (VP2) viruslike particles (VLPs), all 69 healthy adults who were studied were found to be positive (seropositive) for HBoV-specific IgG antibodies by ELISA. Using HBoV VLPs, we observed frequent VP2-specific humoral immune responses in healthy adults, whereas seronegative status was predominantly detected in young children (age, 2 years) [27, 37] .	./cache/cord-007187-gb1txu1o.txt	./txt/cord-007187-gb1txu1o.txt