key: cord-302125-96w0nh9q
authors: Péré, Hélène; Wack, Maxime; Védie, Benoit; Guinet, Nathalie Demory; Najiby, Kassis Chikani; Janot, Laurence; Bélec, Laurent; Veyer, David
title: Sequential SARS-CoV-2 IgG assays as confirmatory strategy to confirm equivocal results: Hospital-wide antibody screening in 3,569 staff health care workers in Paris
date: 2020-09-03
journal: J Clin Virol
DOI: 10.1016/j.jcv.2020.104617
sha: 
doc_id: 302125
cord_uid: 96w0nh9q

nan

Sequential SARS-CoV-2 IgG assays as confirmatory strategy to confirm equivocal results: Hospital-wide antibody screening in 3,569 staff health care workers in Paris Running title: Sequential SARS-CoV-2 serology testing in health-workers Hélène Péré 1,2,3 , Maxime Wack 4 , Benoit Védie 5 , Nathalie Demory Guinet 6 , Kassis Chikani Najiby 7 , Laurence Janot 6 , Laurent Bélec 1,2,3 , David Veyer 1, 8 surface protein, with the high-throughput UniCel DxI 800 Access Immunoassay System (Beckman Coulter), to increase hospital productivity in SARS-CoV-2 IgG serology testing.

The analytical performance of the Beckman Coulter assay was assessed using serum samples obtained from inpatients with COVID-19 confirmed by molecular diagnosis at least 4 weeks ago (n=104) and from patients randomly selected for whom serum samples were collected before the COVID-19 outbreak (n=56). The ACCESS SARS-CoV-2 IgG assay showed sensitivity of 93% (95% CI: 86%-97%), and specificity of 100% (95% CI: 93%-100%), demonstrating high analytical performances allowing convenient management of suspected on-going and past-infections, similar to the Abbott SARS-CoV-2 IgG assay.

In France, the end of confinement took place on May 11 th , 2020. The virology laboratory of Hôpital Européen Georges Pompidou was then called upon to test all hospital staff exposed to the virus during the epidemic peak. From May 2 sd to 26 th June 2020, we 

Unité de Génomique Fonctionnelle des Tumeurs Solides

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The authors gratefully thank the department of clinical biochemistry, Hôpital Européen Georges Pompidou, Paris, especially the technicians from the serological platform. The authors are also grateful to Beckman Coulter, France, for providing the ACCESS SARS-CoV-2 IgG kits for the study.J o u r n a l P r e -p r o o f