id	sid	tid	token	lemma	pos
cord-277909-rn1dow26	1	1	key	key	JJ
cord-277909-rn1dow26	1	2	:	:	:
cord-277909-rn1dow26	1	3	cord-277909-rn1dow26	cord-277909-rn1dow26	NNS
cord-277909-rn1dow26	1	4	authors	author	NNS
cord-277909-rn1dow26	1	5	:	:	:
cord-277909-rn1dow26	1	6	Gunson	Gunson	NNP
cord-277909-rn1dow26	1	7	,	,	,
cord-277909-rn1dow26	1	8	R.N.	R.N.	NNP
cord-277909-rn1dow26	1	9	;	;	:
cord-277909-rn1dow26	1	10	Collins	Collins	NNP
cord-277909-rn1dow26	1	11	,	,	,
cord-277909-rn1dow26	1	12	T.C.	T.C.	NNP
cord-277909-rn1dow26	1	13	;	;	:
cord-277909-rn1dow26	1	14	Carman	Carman	NNP
cord-277909-rn1dow26	1	15	,	,	,
cord-277909-rn1dow26	1	16	W.F.	W.F.	NNP
cord-277909-rn1dow26	1	17	title	title	NN
cord-277909-rn1dow26	1	18	:	:	:
cord-277909-rn1dow26	1	19	Practical	practical	JJ
cord-277909-rn1dow26	1	20	experience	experience	NN
cord-277909-rn1dow26	1	21	of	of	IN
cord-277909-rn1dow26	1	22	high	high	JJ
cord-277909-rn1dow26	1	23	throughput	throughput	NN
cord-277909-rn1dow26	2	1	real	real	JJ
cord-277909-rn1dow26	2	2	time	time	NN
cord-277909-rn1dow26	2	3	PCR	PCR	NNP
cord-277909-rn1dow26	2	4	in	in	IN
cord-277909-rn1dow26	2	5	the	the	DT
cord-277909-rn1dow26	2	6	routine	routine	JJ
cord-277909-rn1dow26	2	7	diagnostic	diagnostic	JJ
cord-277909-rn1dow26	2	8	virology	virology	NN
cord-277909-rn1dow26	2	9	setting	set	VBG
cord-277909-rn1dow26	2	10	date	date	NN
cord-277909-rn1dow26	2	11	:	:	:
cord-277909-rn1dow26	2	12	2006	2006	CD
cord-277909-rn1dow26	2	13	-	-	HYPH
cord-277909-rn1dow26	2	14	02	02	CD
cord-277909-rn1dow26	2	15	-	-	HYPH
cord-277909-rn1dow26	2	16	07	07	CD
cord-277909-rn1dow26	2	17	journal	journal	NN
cord-277909-rn1dow26	2	18	:	:	:
cord-277909-rn1dow26	3	1	J	J	NNP
cord-277909-rn1dow26	3	2	Clin	Clin	NNP
cord-277909-rn1dow26	3	3	Virol	Virol	NNP
cord-277909-rn1dow26	3	4	DOI	DOI	NNP
cord-277909-rn1dow26	3	5	:	:	:
cord-277909-rn1dow26	3	6	10.1016/	10.1016/	CD
cord-277909-rn1dow26	4	1	j.jcv.2005.12.006	j.jcv.2005.12.006	NNP
cord-277909-rn1dow26	4	2	sha	sha	NNP
cord-277909-rn1dow26	4	3	:	:	:
cord-277909-rn1dow26	5	1	779edc3dcfe107c480ade1d87c841a6e11e31177	779edc3dcfe107c480ade1d87c841a6e11e31177	DT
cord-277909-rn1dow26	5	2	doc_id	doc_id	CD
cord-277909-rn1dow26	5	3	:	:	:
cord-277909-rn1dow26	6	1	277909	277909	CD
cord-277909-rn1dow26	6	2	cord_uid	cord_uid	NNS
cord-277909-rn1dow26	6	3	:	:	:
cord-277909-rn1dow26	6	4	rn1dow26	rn1dow26	NNS
cord-277909-rn1dow26	7	1	The	the	DT
cord-277909-rn1dow26	7	2	advent	advent	NN
cord-277909-rn1dow26	7	3	of	of	IN
cord-277909-rn1dow26	7	4	PCR	PCR	NNP
cord-277909-rn1dow26	7	5	has	have	VBZ
cord-277909-rn1dow26	7	6	transformed	transform	VBN
cord-277909-rn1dow26	7	7	the	the	DT
cord-277909-rn1dow26	7	8	utility	utility	NN
cord-277909-rn1dow26	7	9	of	of	IN
cord-277909-rn1dow26	7	10	the	the	DT
cord-277909-rn1dow26	7	11	virus	virus	NN
cord-277909-rn1dow26	7	12	diagnostic	diagnostic	JJ
cord-277909-rn1dow26	7	13	laboratory	laboratory	NN
cord-277909-rn1dow26	7	14	.	.	.
cord-277909-rn1dow26	8	1	In	in	IN
cord-277909-rn1dow26	8	2	comparison	comparison	NN
cord-277909-rn1dow26	8	3	to	to	IN
cord-277909-rn1dow26	8	4	traditional	traditional	JJ
cord-277909-rn1dow26	8	5	gel	gel	NN
cord-277909-rn1dow26	8	6	based	base	VBN
cord-277909-rn1dow26	8	7	PCR	PCR	NNP
cord-277909-rn1dow26	8	8	assays	assay	NNS
cord-277909-rn1dow26	8	9	,	,	,
cord-277909-rn1dow26	8	10	real	real	JJ
cord-277909-rn1dow26	8	11	time	time	NN
cord-277909-rn1dow26	8	12	PCR	PCR	NNP
cord-277909-rn1dow26	8	13	offers	offer	VBZ
cord-277909-rn1dow26	8	14	increased	increase	VBN
cord-277909-rn1dow26	8	15	sensitivity	sensitivity	NN
cord-277909-rn1dow26	8	16	and	and	CC
cord-277909-rn1dow26	8	17	specificity	specificity	NN
cord-277909-rn1dow26	8	18	in	in	IN
cord-277909-rn1dow26	8	19	a	a	DT
cord-277909-rn1dow26	8	20	rapid	rapid	JJ
cord-277909-rn1dow26	8	21	format	format	NN
cord-277909-rn1dow26	8	22	.	.	.
cord-277909-rn1dow26	9	1	Over	over	IN
cord-277909-rn1dow26	9	2	the	the	DT
cord-277909-rn1dow26	9	3	past	past	JJ
cord-277909-rn1dow26	9	4	4	4	CD
cord-277909-rn1dow26	9	5	years	year	NNS
cord-277909-rn1dow26	9	6	,	,	,
cord-277909-rn1dow26	9	7	we	-PRON-	PRP
cord-277909-rn1dow26	9	8	have	have	VBP
cord-277909-rn1dow26	9	9	introduced	introduce	VBN
cord-277909-rn1dow26	9	10	a	a	DT
cord-277909-rn1dow26	9	11	number	number	NN
cord-277909-rn1dow26	9	12	of	of	IN
cord-277909-rn1dow26	9	13	qualitative	qualitative	JJ
cord-277909-rn1dow26	9	14	and	and	CC
cord-277909-rn1dow26	9	15	quantitative	quantitative	JJ
cord-277909-rn1dow26	9	16	real	real	JJ
cord-277909-rn1dow26	9	17	time	time	NN
cord-277909-rn1dow26	9	18	PCR	pcr	NN
cord-277909-rn1dow26	9	19	assays	assay	NNS
cord-277909-rn1dow26	9	20	into	into	IN
cord-277909-rn1dow26	9	21	our	-PRON-	PRP$
cord-277909-rn1dow26	9	22	routine	routine	JJ
cord-277909-rn1dow26	9	23	testing	testing	NN
cord-277909-rn1dow26	9	24	service	service	NN
cord-277909-rn1dow26	9	25	.	.	.
cord-277909-rn1dow26	10	1	During	during	IN
cord-277909-rn1dow26	10	2	this	this	DT
cord-277909-rn1dow26	10	3	period	period	NN
cord-277909-rn1dow26	10	4	,	,	,
cord-277909-rn1dow26	10	5	we	-PRON-	PRP
cord-277909-rn1dow26	10	6	have	have	VBP
cord-277909-rn1dow26	10	7	gained	gain	VBN
cord-277909-rn1dow26	10	8	substantial	substantial	JJ
cord-277909-rn1dow26	10	9	experience	experience	NN
cord-277909-rn1dow26	10	10	relating	relate	VBG
cord-277909-rn1dow26	10	11	to	to	IN
cord-277909-rn1dow26	10	12	the	the	DT
cord-277909-rn1dow26	10	13	development	development	NN
cord-277909-rn1dow26	10	14	and	and	CC
cord-277909-rn1dow26	10	15	implementation	implementation	NN
cord-277909-rn1dow26	10	16	of	of	IN
cord-277909-rn1dow26	10	17	real	real	JJ
cord-277909-rn1dow26	10	18	-	-	HYPH
cord-277909-rn1dow26	10	19	time	time	NN
cord-277909-rn1dow26	10	20	assays	assay	NNS
cord-277909-rn1dow26	10	21	.	.	.
cord-277909-rn1dow26	11	1	Furthermore	furthermore	RB
cord-277909-rn1dow26	11	2	,	,	,
cord-277909-rn1dow26	11	3	we	-PRON-	PRP
cord-277909-rn1dow26	11	4	have	have	VBP
cord-277909-rn1dow26	11	5	developed	develop	VBN
cord-277909-rn1dow26	11	6	strategies	strategy	NNS
cord-277909-rn1dow26	11	7	that	that	WDT
cord-277909-rn1dow26	11	8	have	have	VBP
cord-277909-rn1dow26	11	9	allowed	allow	VBN
cord-277909-rn1dow26	11	10	us	-PRON-	PRP
cord-277909-rn1dow26	11	11	to	to	TO
cord-277909-rn1dow26	11	12	increase	increase	VB
cord-277909-rn1dow26	11	13	our	-PRON-	PRP$
cord-277909-rn1dow26	11	14	sample	sample	NN
cord-277909-rn1dow26	11	15	throughput	throughput	NN
cord-277909-rn1dow26	11	16	while	while	IN
cord-277909-rn1dow26	11	17	maintaining	maintain	VBG
cord-277909-rn1dow26	11	18	or	or	CC
cord-277909-rn1dow26	11	19	even	even	RB
cord-277909-rn1dow26	11	20	reducing	reduce	VBG
cord-277909-rn1dow26	11	21	turn	turn	NN
cord-277909-rn1dow26	11	22	around	around	RP
cord-277909-rn1dow26	11	23	times	time	NNS
cord-277909-rn1dow26	11	24	.	.	.
cord-277909-rn1dow26	12	1	The	the	DT
cord-277909-rn1dow26	12	2	issues	issue	NNS
cord-277909-rn1dow26	12	3	resulting	result	VBG
cord-277909-rn1dow26	12	4	from	from	IN
cord-277909-rn1dow26	12	5	this	this	DT
cord-277909-rn1dow26	12	6	experience	experience	NN
cord-277909-rn1dow26	12	7	(	(	-LRB-
cord-277909-rn1dow26	12	8	some	some	DT
cord-277909-rn1dow26	12	9	of	of	IN
cord-277909-rn1dow26	12	10	it	-PRON-	PRP
cord-277909-rn1dow26	12	11	bad	bad	JJ
cord-277909-rn1dow26	12	12	)	)	-RRB-
cord-277909-rn1dow26	12	13	are	be	VBP
cord-277909-rn1dow26	12	14	discussed	discuss	VBN
cord-277909-rn1dow26	12	15	in	in	IN
cord-277909-rn1dow26	12	16	detail	detail	NN
cord-277909-rn1dow26	12	17	with	with	IN
cord-277909-rn1dow26	12	18	the	the	DT
cord-277909-rn1dow26	12	19	aim	aim	NN
cord-277909-rn1dow26	12	20	of	of	IN
cord-277909-rn1dow26	12	21	informing	inform	VBG
cord-277909-rn1dow26	12	22	laboratories	laboratory	NNS
cord-277909-rn1dow26	12	23	that	that	WDT
cord-277909-rn1dow26	12	24	are	be	VBP
cord-277909-rn1dow26	12	25	only	only	RB
cord-277909-rn1dow26	12	26	just	just	RB
cord-277909-rn1dow26	12	27	beginning	begin	VBG
cord-277909-rn1dow26	12	28	to	to	TO
cord-277909-rn1dow26	12	29	investigate	investigate	VB
cord-277909-rn1dow26	12	30	the	the	DT
cord-277909-rn1dow26	12	31	potential	potential	NN
cord-277909-rn1dow26	12	32	of	of	IN
cord-277909-rn1dow26	12	33	this	this	DT
cord-277909-rn1dow26	12	34	technology	technology	NN
cord-277909-rn1dow26	12	35	.	.	.
cord-277909-rn1dow26	13	1	The	the	DT
cord-277909-rn1dow26	13	2	advent	advent	NN
cord-277909-rn1dow26	13	3	of	of	IN
cord-277909-rn1dow26	13	4	polymerase	polymerase	NN
cord-277909-rn1dow26	13	5	chain	chain	NN
cord-277909-rn1dow26	13	6	reaction	reaction	NN
cord-277909-rn1dow26	13	7	(	(	-LRB-
cord-277909-rn1dow26	13	8	PCR	PCR	NNP
cord-277909-rn1dow26	13	9	)	)	-RRB-
cord-277909-rn1dow26	13	10	has	have	VBZ
cord-277909-rn1dow26	13	11	transformed	transform	VBN
cord-277909-rn1dow26	13	12	the	the	DT
cord-277909-rn1dow26	13	13	utility	utility	NN
cord-277909-rn1dow26	13	14	of	of	IN
cord-277909-rn1dow26	13	15	the	the	DT
cord-277909-rn1dow26	13	16	virus	virus	NN
cord-277909-rn1dow26	13	17	diagnostic	diagnostic	JJ
cord-277909-rn1dow26	13	18	laboratory	laboratory	NN
cord-277909-rn1dow26	13	19	.	.	.
cord-277909-rn1dow26	14	1	In	in	IN
cord-277909-rn1dow26	14	2	comparison	comparison	NN
cord-277909-rn1dow26	14	3	with	with	IN
cord-277909-rn1dow26	14	4	traditional	traditional	JJ
cord-277909-rn1dow26	14	5	methods	method	NNS
cord-277909-rn1dow26	14	6	,	,	,
cord-277909-rn1dow26	14	7	PCR	PCR	NNP
cord-277909-rn1dow26	14	8	offers	offer	VBZ
cord-277909-rn1dow26	14	9	a	a	DT
cord-277909-rn1dow26	14	10	highly	highly	RB
cord-277909-rn1dow26	14	11	sensitive	sensitive	JJ
cord-277909-rn1dow26	14	12	and	and	CC
cord-277909-rn1dow26	14	13	specific	specific	JJ
cord-277909-rn1dow26	14	14	result	result	NN
cord-277909-rn1dow26	14	15	within	within	IN
cord-277909-rn1dow26	14	16	24	24	CD
cord-277909-rn1dow26	14	17	-	-	SYM
cord-277909-rn1dow26	14	18	48	48	CD
cord-277909-rn1dow26	14	19	h.	h.	NN
cord-277909-rn1dow26	15	1	The	the	DT
cord-277909-rn1dow26	15	2	routine	routine	JJ
cord-277909-rn1dow26	15	3	use	use	NN
cord-277909-rn1dow26	15	4	of	of	IN
cord-277909-rn1dow26	15	5	this	this	DT
cord-277909-rn1dow26	15	6	test	test	NN
cord-277909-rn1dow26	15	7	in	in	IN
cord-277909-rn1dow26	15	8	diagnostic	diagnostic	JJ
cord-277909-rn1dow26	15	9	laboratories	laboratory	NNS
cord-277909-rn1dow26	15	10	has	have	VBZ
cord-277909-rn1dow26	15	11	led	lead	VBN
cord-277909-rn1dow26	15	12	to	to	IN
cord-277909-rn1dow26	15	13	many	many	JJ
cord-277909-rn1dow26	15	14	benefits	benefit	NNS
cord-277909-rn1dow26	15	15	including	include	VBG
cord-277909-rn1dow26	15	16	improved	improve	VBN
cord-277909-rn1dow26	15	17	patient	patient	JJ
cord-277909-rn1dow26	15	18	management	management	NN
cord-277909-rn1dow26	15	19	,	,	,
cord-277909-rn1dow26	15	20	and	and	CC
cord-277909-rn1dow26	15	21	increased	increase	VBN
cord-277909-rn1dow26	15	22	ascertainment	ascertainment	NN
cord-277909-rn1dow26	15	23	of	of	IN
cord-277909-rn1dow26	15	24	previously	previously	RB
cord-277909-rn1dow26	15	25	under	under	RB
cord-277909-rn1dow26	15	26	-	-	HYPH
cord-277909-rn1dow26	15	27	diagnosed	diagnose	VBN
cord-277909-rn1dow26	15	28	and	and	CC
cord-277909-rn1dow26	15	29	undetectable	undetectable	JJ
cord-277909-rn1dow26	15	30	viruses	virus	NNS
cord-277909-rn1dow26	15	31	.	.	.
cord-277909-rn1dow26	16	1	The	the	DT
cord-277909-rn1dow26	16	2	advent	advent	NN
cord-277909-rn1dow26	16	3	of	of	IN
cord-277909-rn1dow26	16	4	real	real	JJ
cord-277909-rn1dow26	16	5	time	time	NN
cord-277909-rn1dow26	16	6	PCR	PCR	NNP
cord-277909-rn1dow26	16	7	technologies	technology	NNS
cord-277909-rn1dow26	16	8	has	have	VBZ
cord-277909-rn1dow26	16	9	further	further	RB
cord-277909-rn1dow26	16	10	improved	improve	VBN
cord-277909-rn1dow26	16	11	upon	upon	IN
cord-277909-rn1dow26	16	12	these	these	DT
cord-277909-rn1dow26	16	13	already	already	RB
cord-277909-rn1dow26	16	14	significant	significant	JJ
cord-277909-rn1dow26	16	15	benefits	benefit	NNS
cord-277909-rn1dow26	16	16	(	(	-LRB-
cord-277909-rn1dow26	16	17	Arya	Arya	NNP
cord-277909-rn1dow26	16	18	et	et	NNP
cord-277909-rn1dow26	16	19	al	al	NNP
cord-277909-rn1dow26	16	20	.	.	NNP
cord-277909-rn1dow26	16	21	,	,	,
cord-277909-rn1dow26	16	22	2005	2005	CD
cord-277909-rn1dow26	16	23	;	;	:
cord-277909-rn1dow26	16	24	Aslanzadeh	Aslanzadeh	NNP
cord-277909-rn1dow26	16	25	,	,	,
cord-277909-rn1dow26	16	26	2004	2004	CD
cord-277909-rn1dow26	16	27	;	;	:
cord-277909-rn1dow26	16	28	Bustin	Bustin	NNP
cord-277909-rn1dow26	16	29	and	and	CC
cord-277909-rn1dow26	16	30	Nolan	Nolan	NNP
cord-277909-rn1dow26	16	31	,	,	,
cord-277909-rn1dow26	16	32	2004	2004	CD
cord-277909-rn1dow26	16	33	;	;	:
cord-277909-rn1dow26	16	34	Mackay	Mackay	NNP
cord-277909-rn1dow26	16	35	,	,	,
cord-277909-rn1dow26	16	36	2004	2004	CD
cord-277909-rn1dow26	16	37	;	;	:
cord-277909-rn1dow26	16	38	Tan	Tan	NNP
cord-277909-rn1dow26	16	39	et	et	FW
cord-277909-rn1dow26	16	40	al	al	NNP
cord-277909-rn1dow26	16	41	.	.	NNP
cord-277909-rn1dow26	16	42	,	,	,
cord-277909-rn1dow26	16	43	2004	2004	CD
cord-277909-rn1dow26	16	44	)	)	-RRB-
cord-277909-rn1dow26	16	45	.	.	.
cord-277909-rn1dow26	17	1	In	in	IN
cord-277909-rn1dow26	17	2	comparison	comparison	NN
cord-277909-rn1dow26	17	3	to	to	IN
cord-277909-rn1dow26	17	4	traditional	traditional	JJ
cord-277909-rn1dow26	17	5	gel	gel	NN
cord-277909-rn1dow26	17	6	-	-	HYPH
cord-277909-rn1dow26	17	7	based	base	VBN
cord-277909-rn1dow26	17	8	PCR	PCR	NNP
cord-277909-rn1dow26	17	9	assays	assay	NNS
cord-277909-rn1dow26	17	10	,	,	,
cord-277909-rn1dow26	17	11	real	real	JJ
cord-277909-rn1dow26	17	12	time	time	NN
cord-277909-rn1dow26	17	13	PCR	PCR	NNP
cord-277909-rn1dow26	17	14	offers	offer	VBZ
cord-277909-rn1dow26	17	15	increased	increase	VBN
cord-277909-rn1dow26	17	16	sensitivity	sensitivity	NN
cord-277909-rn1dow26	17	17	and	and	CC
cord-277909-rn1dow26	17	18	specificity	specificity	NN
cord-277909-rn1dow26	17	19	in	in	IN
cord-277909-rn1dow26	17	20	a	a	DT
cord-277909-rn1dow26	17	21	rapid	rapid	JJ
cord-277909-rn1dow26	17	22	format	format	NN
cord-277909-rn1dow26	17	23	(	(	-LRB-
cord-277909-rn1dow26	17	24	turn	turn	VB
cord-277909-rn1dow26	17	25	around	around	IN
cord-277909-rn1dow26	17	26	time	time	NN
cord-277909-rn1dow26	17	27	from	from	IN
cord-277909-rn1dow26	17	28	sample	sample	NN
cord-277909-rn1dow26	17	29	receipt	receipt	NN
cord-277909-rn1dow26	17	30	to	to	TO
cord-277909-rn1dow26	17	31	result	result	VB
cord-277909-rn1dow26	17	32	<	<	NNP
cord-277909-rn1dow26	17	33	5	5	CD
cord-277909-rn1dow26	17	34	h	h	NN
cord-277909-rn1dow26	17	35	)	)	-RRB-
cord-277909-rn1dow26	17	36	.	.	.
cord-277909-rn1dow26	18	1	Unlike	unlike	IN
cord-277909-rn1dow26	18	2	traditional	traditional	JJ
cord-277909-rn1dow26	18	3	systems	system	NNS
cord-277909-rn1dow26	18	4	,	,	,
cord-277909-rn1dow26	18	5	which	which	WDT
cord-277909-rn1dow26	18	6	rely	rely	VBP
cord-277909-rn1dow26	18	7	upon	upon	IN
cord-277909-rn1dow26	18	8	endpoint	endpoint	NN
cord-277909-rn1dow26	18	9	analysis	analysis	NN
cord-277909-rn1dow26	18	10	,	,	,
cord-277909-rn1dow26	18	11	real	real	JJ
cord-277909-rn1dow26	18	12	time	time	NN
cord-277909-rn1dow26	18	13	PCR	PCR	NNP
cord-277909-rn1dow26	18	14	assays	assay	NNS
cord-277909-rn1dow26	18	15	visualise	visualise	VBP
cord-277909-rn1dow26	18	16	the	the	DT
cord-277909-rn1dow26	18	17	reaction	reaction	NN
cord-277909-rn1dow26	18	18	as	as	IN
cord-277909-rn1dow26	18	19	it	-PRON-	PRP
cord-277909-rn1dow26	18	20	is	be	VBZ
cord-277909-rn1dow26	18	21	taking	take	VBG
cord-277909-rn1dow26	18	22	place	place	NN
cord-277909-rn1dow26	18	23	allowing	allow	VBG
cord-277909-rn1dow26	18	24	quantification	quantification	NN
cord-277909-rn1dow26	18	25	and	and	CC
cord-277909-rn1dow26	18	26	reaction	reaction	NN
cord-277909-rn1dow26	18	27	analysis	analysis	NN
cord-277909-rn1dow26	18	28	(	(	-LRB-
cord-277909-rn1dow26	18	29	e.g.	e.g.	RB
cord-277909-rn1dow26	18	30	,	,	,
cord-277909-rn1dow26	18	31	PCR	PCR	NNP
cord-277909-rn1dow26	18	32	efficiency	efficiency	NN
cord-277909-rn1dow26	18	33	)	)	-RRB-
cord-277909-rn1dow26	18	34	.	.	.
cord-277909-rn1dow26	19	1	Since	since	IN
cord-277909-rn1dow26	19	2	real	real	JJ
cord-277909-rn1dow26	19	3	time	time	NN
cord-277909-rn1dow26	19	4	PCR	PCR	NNP
cord-277909-rn1dow26	19	5	reactions	reaction	NNS
cord-277909-rn1dow26	19	6	are	be	VBP
cord-277909-rn1dow26	19	7	performed	perform	VBN
cord-277909-rn1dow26	19	8	in	in	IN
cord-277909-rn1dow26	19	9	a	a	DT
cord-277909-rn1dow26	19	10	closed	closed	JJ
cord-277909-rn1dow26	19	11	system	system	NN
cord-277909-rn1dow26	19	12	(	(	-LRB-
cord-277909-rn1dow26	19	13	no	no	DT
cord-277909-rn1dow26	19	14	gel	gel	NN
cord-277909-rn1dow26	19	15	analysis	analysis	NN
cord-277909-rn1dow26	19	16	needed	need	VBN
cord-277909-rn1dow26	19	17	)	)	-RRB-
cord-277909-rn1dow26	19	18	the	the	DT
cord-277909-rn1dow26	19	19	risk	risk	NN
cord-277909-rn1dow26	19	20	of	of	IN
cord-277909-rn1dow26	19	21	contamination	contamination	NN
cord-277909-rn1dow26	19	22	has	have	VBZ
cord-277909-rn1dow26	19	23	been	be	VBN
cord-277909-rn1dow26	19	24	substantially	substantially	RB
cord-277909-rn1dow26	19	25	reduced	reduce	VBN
cord-277909-rn1dow26	19	26	.	.	.
cord-277909-rn1dow26	20	1	This	this	DT
cord-277909-rn1dow26	20	2	has	have	VBZ
cord-277909-rn1dow26	20	3	also	also	RB
cord-277909-rn1dow26	20	4	reduced	reduce	VBN
cord-277909-rn1dow26	20	5	the	the	DT
cord-277909-rn1dow26	20	6	requirement	requirement	NN
cord-277909-rn1dow26	20	7	for	for	IN
cord-277909-rn1dow26	20	8	a	a	DT
cord-277909-rn1dow26	20	9	stringent	stringent	JJ
cord-277909-rn1dow26	20	10	laboratory	laboratory	NN
cord-277909-rn1dow26	20	11	structure	structure	NN
cord-277909-rn1dow26	20	12	.	.	.
cord-277909-rn1dow26	21	1	The	the	DT
cord-277909-rn1dow26	21	2	increasing	increase	VBG
cord-277909-rn1dow26	21	3	number	number	NN
cord-277909-rn1dow26	21	4	of	of	IN
cord-277909-rn1dow26	21	5	chemistries	chemistry	NNS
cord-277909-rn1dow26	21	6	and	and	CC
cord-277909-rn1dow26	21	7	platforms	platform	NNS
cord-277909-rn1dow26	21	8	available	available	JJ
cord-277909-rn1dow26	21	9	for	for	IN
cord-277909-rn1dow26	21	10	real	real	JJ
cord-277909-rn1dow26	21	11	time	time	NN
cord-277909-rn1dow26	21	12	PCR	PCR	NNP
cord-277909-rn1dow26	21	13	have	have	VBP
cord-277909-rn1dow26	21	14	reduced	reduce	VBN
cord-277909-rn1dow26	21	15	its	-PRON-	PRP$
cord-277909-rn1dow26	21	16	overall	overall	JJ
cord-277909-rn1dow26	21	17	cost	cost	NN
cord-277909-rn1dow26	21	18	significantly	significantly	RB
cord-277909-rn1dow26	21	19	making	make	VBG
cord-277909-rn1dow26	21	20	this	this	DT
cord-277909-rn1dow26	21	21	an	an	DT
cord-277909-rn1dow26	21	22	increasingly	increasingly	RB
cord-277909-rn1dow26	21	23	attractive	attractive	JJ
cord-277909-rn1dow26	21	24	technique	technique	NN
cord-277909-rn1dow26	21	25	.	.	.
cord-277909-rn1dow26	22	1	Over	over	IN
cord-277909-rn1dow26	22	2	the	the	DT
cord-277909-rn1dow26	22	3	past	past	JJ
cord-277909-rn1dow26	22	4	4	4	CD
cord-277909-rn1dow26	22	5	years	year	NNS
cord-277909-rn1dow26	22	6	we	-PRON-	PRP
cord-277909-rn1dow26	22	7	have	have	VBP
cord-277909-rn1dow26	22	8	introduced	introduce	VBN
cord-277909-rn1dow26	22	9	a	a	DT
cord-277909-rn1dow26	22	10	number	number	NN
cord-277909-rn1dow26	22	11	of	of	IN
cord-277909-rn1dow26	22	12	qualitative	qualitative	JJ
cord-277909-rn1dow26	22	13	and	and	CC
cord-277909-rn1dow26	22	14	quantitative	quantitative	JJ
cord-277909-rn1dow26	22	15	real	real	JJ
cord-277909-rn1dow26	22	16	time	time	NN
cord-277909-rn1dow26	22	17	PCR	pcr	NN
cord-277909-rn1dow26	22	18	assays	assay	NNS
cord-277909-rn1dow26	22	19	into	into	IN
cord-277909-rn1dow26	22	20	our	-PRON-	PRP$
cord-277909-rn1dow26	22	21	routine	routine	JJ
cord-277909-rn1dow26	22	22	testing	testing	NN
cord-277909-rn1dow26	22	23	service	service	NN
cord-277909-rn1dow26	22	24	.	.	.
cord-277909-rn1dow26	23	1	These	these	DT
cord-277909-rn1dow26	23	2	include	include	VBP
cord-277909-rn1dow26	23	3	assays	assay	NNS
cord-277909-rn1dow26	23	4	for	for	IN
cord-277909-rn1dow26	23	5	the	the	DT
cord-277909-rn1dow26	23	6	detection	detection	NN
cord-277909-rn1dow26	23	7	of	of	IN
cord-277909-rn1dow26	23	8	influenza	influenza	NN
cord-277909-rn1dow26	23	9	A	a	NN
cord-277909-rn1dow26	23	10	,	,	,
cord-277909-rn1dow26	23	11	B	B	NNP
cord-277909-rn1dow26	23	12	and	and	CC
cord-277909-rn1dow26	23	13	C	C	NNP
cord-277909-rn1dow26	23	14	,	,	,
cord-277909-rn1dow26	23	15	human	human	JJ
cord-277909-rn1dow26	23	16	metapneumovirus	metapneumovirus	NN
cord-277909-rn1dow26	23	17	,	,	,
cord-277909-rn1dow26	23	18	respiratory	respiratory	JJ
cord-277909-rn1dow26	23	19	syncytial	syncytial	JJ
cord-277909-rn1dow26	23	20	viruses	virus	NNS
cord-277909-rn1dow26	23	21	(	(	-LRB-
cord-277909-rn1dow26	23	22	RSV	RSV	NNP
cord-277909-rn1dow26	23	23	)	)	-RRB-
cord-277909-rn1dow26	23	24	A	a	NN
cord-277909-rn1dow26	23	25	and	and	CC
cord-277909-rn1dow26	23	26	B	b	NN
cord-277909-rn1dow26	23	27	,	,	,
cord-277909-rn1dow26	23	28	rhinovirus	rhinovirus	NN
cord-277909-rn1dow26	23	29	,	,	,
cord-277909-rn1dow26	23	30	parainfluenza	parainfluenza	NNP
cord-277909-rn1dow26	23	31	viruses	virus	NNS
cord-277909-rn1dow26	23	32	1	1	CD
cord-277909-rn1dow26	23	33	-	-	SYM
cord-277909-rn1dow26	23	34	4	4	CD
cord-277909-rn1dow26	23	35	,	,	,
cord-277909-rn1dow26	23	36	coronaviruses	coronaviruse	NNS
cord-277909-rn1dow26	23	37	NL63	NL63	NNP
cord-277909-rn1dow26	23	38	,	,	,
cord-277909-rn1dow26	23	39	OC43	OC43	NNP
cord-277909-rn1dow26	23	40	and	and	CC
cord-277909-rn1dow26	23	41	229E	229e	CD
cord-277909-rn1dow26	23	42	,	,	,
cord-277909-rn1dow26	23	43	Chlamydia	Chlamydia	NNP
cord-277909-rn1dow26	23	44	pneumoniae	pneumoniae	NN
cord-277909-rn1dow26	23	45	,	,	,
cord-277909-rn1dow26	23	46	Mycoplasma	mycoplasma	NN
cord-277909-rn1dow26	23	47	pneumoniae	pneumoniae	NN
cord-277909-rn1dow26	23	48	,	,	,
cord-277909-rn1dow26	23	49	Pneumocystis	Pneumocystis	NNP
cord-277909-rn1dow26	23	50	jiroveci	jiroveci	NNP
cord-277909-rn1dow26	23	51	,	,	,
cord-277909-rn1dow26	23	52	varicella	varicella	NN
cord-277909-rn1dow26	23	53	zoster	zoster	NN
cord-277909-rn1dow26	23	54	virus	virus	NN
cord-277909-rn1dow26	23	55	(	(	-LRB-
cord-277909-rn1dow26	23	56	VZV	VZV	NNP
cord-277909-rn1dow26	23	57	)	)	-RRB-
cord-277909-rn1dow26	23	58	,	,	,
cord-277909-rn1dow26	23	59	herpes	herpes	NN
cord-277909-rn1dow26	23	60	simplex	simplex	NNP
cord-277909-rn1dow26	23	61	virus	virus	NN
cord-277909-rn1dow26	23	62	(	(	-LRB-
cord-277909-rn1dow26	23	63	HSV	HSV	NNP
cord-277909-rn1dow26	23	64	)	)	-RRB-
cord-277909-rn1dow26	23	65	1	1	CD
cord-277909-rn1dow26	23	66	and	and	CC
cord-277909-rn1dow26	23	67	2	2	CD
cord-277909-rn1dow26	23	68	,	,	,
cord-277909-rn1dow26	23	69	cytomegalovirus	cytomegalovirus	NNP
cord-277909-rn1dow26	23	70	(	(	-LRB-
cord-277909-rn1dow26	23	71	CMV	CMV	NNP
cord-277909-rn1dow26	23	72	)	)	-RRB-
cord-277909-rn1dow26	23	73	,	,	,
cord-277909-rn1dow26	23	74	Epstien	Epstien	NNP
cord-277909-rn1dow26	23	75	Barr	Barr	NNP
cord-277909-rn1dow26	23	76	virus	virus	NN
cord-277909-rn1dow26	23	77	(	(	-LRB-
cord-277909-rn1dow26	23	78	EBV	EBV	NNP
cord-277909-rn1dow26	23	79	)	)	-RRB-
cord-277909-rn1dow26	23	80	,	,	,
cord-277909-rn1dow26	23	81	HHV-6	HHV-6	NNP
cord-277909-rn1dow26	23	82	,	,	,
cord-277909-rn1dow26	23	83	HHV-7	HHV-7	NNP
cord-277909-rn1dow26	23	84	,	,	,
cord-277909-rn1dow26	23	85	norovirus	norovirus	NN
cord-277909-rn1dow26	23	86	,	,	,
cord-277909-rn1dow26	23	87	adenovirus	adenovirus	NN
cord-277909-rn1dow26	23	88	,	,	,
cord-277909-rn1dow26	23	89	rotavirus	rotavirus	NN
cord-277909-rn1dow26	23	90	,	,	,
cord-277909-rn1dow26	23	91	astrovirus	astrovirus	NNP
cord-277909-rn1dow26	23	92	,	,	,
cord-277909-rn1dow26	23	93	sapovirus	sapovirus	NNP
cord-277909-rn1dow26	23	94	,	,	,
cord-277909-rn1dow26	23	95	erythrovirus	erythrovirus	NNP
cord-277909-rn1dow26	23	96	B19	B19	NNP
cord-277909-rn1dow26	23	97	,	,	,
cord-277909-rn1dow26	23	98	mumps	mumps	NN
cord-277909-rn1dow26	23	99	,	,	,
cord-277909-rn1dow26	23	100	Chlamydia	Chlamydia	NNP
cord-277909-rn1dow26	23	101	trachomatis	trachomatis	NN
cord-277909-rn1dow26	23	102	,	,	,
cord-277909-rn1dow26	24	1	Mycoplasma	mycoplasma	NN
cord-277909-rn1dow26	24	2	genitalium	genitalium	NN
cord-277909-rn1dow26	24	3	,	,	,
cord-277909-rn1dow26	24	4	Nesseria	Nesseria	NNP
cord-277909-rn1dow26	24	5	gonnorhoeae	gonnorhoeae	NN
cord-277909-rn1dow26	24	6	and	and	CC
cord-277909-rn1dow26	24	7	enterovirus	enterovirus	NN
cord-277909-rn1dow26	24	8	.	.	.
cord-277909-rn1dow26	25	1	Each	each	DT
cord-277909-rn1dow26	25	2	year	year	NN
cord-277909-rn1dow26	25	3	we	-PRON-	PRP
cord-277909-rn1dow26	25	4	carry	carry	VBP
cord-277909-rn1dow26	25	5	out	out	RP
cord-277909-rn1dow26	25	6	more	more	JJR
cord-277909-rn1dow26	25	7	than	than	IN
cord-277909-rn1dow26	25	8	84,000	84,000	CD
cord-277909-rn1dow26	25	9	PCR	PCR	NNP
cord-277909-rn1dow26	25	10	tests	test	NNS
cord-277909-rn1dow26	25	11	.	.	.
cord-277909-rn1dow26	26	1	During	during	IN
cord-277909-rn1dow26	26	2	this	this	DT
cord-277909-rn1dow26	26	3	period	period	NN
cord-277909-rn1dow26	26	4	we	-PRON-	PRP
cord-277909-rn1dow26	26	5	have	have	VBP
cord-277909-rn1dow26	26	6	gained	gain	VBN
cord-277909-rn1dow26	26	7	substantial	substantial	JJ
cord-277909-rn1dow26	26	8	experience	experience	NN
cord-277909-rn1dow26	26	9	relating	relate	VBG
cord-277909-rn1dow26	26	10	to	to	IN
cord-277909-rn1dow26	26	11	the	the	DT
cord-277909-rn1dow26	26	12	development	development	NN
cord-277909-rn1dow26	26	13	and	and	CC
cord-277909-rn1dow26	26	14	implementation	implementation	NN
cord-277909-rn1dow26	26	15	of	of	IN
cord-277909-rn1dow26	26	16	real	real	JJ
cord-277909-rn1dow26	26	17	time	time	NN
cord-277909-rn1dow26	26	18	assays	assay	NNS
cord-277909-rn1dow26	26	19	.	.	.
cord-277909-rn1dow26	27	1	Furthermore	furthermore	RB
cord-277909-rn1dow26	27	2	we	-PRON-	PRP
cord-277909-rn1dow26	27	3	have	have	VBP
cord-277909-rn1dow26	27	4	developed	develop	VBN
cord-277909-rn1dow26	27	5	strategies	strategy	NNS
cord-277909-rn1dow26	27	6	that	that	WDT
cord-277909-rn1dow26	27	7	have	have	VBP
cord-277909-rn1dow26	27	8	allowed	allow	VBN
cord-277909-rn1dow26	27	9	us	-PRON-	PRP
cord-277909-rn1dow26	27	10	to	to	TO
cord-277909-rn1dow26	27	11	increase	increase	VB
cord-277909-rn1dow26	27	12	our	-PRON-	PRP$
cord-277909-rn1dow26	27	13	sample	sample	NN
cord-277909-rn1dow26	27	14	throughput	throughput	NN
cord-277909-rn1dow26	27	15	while	while	IN
cord-277909-rn1dow26	27	16	maintaining	maintain	VBG
cord-277909-rn1dow26	27	17	or	or	CC
cord-277909-rn1dow26	27	18	even	even	RB
cord-277909-rn1dow26	27	19	reducing	reduce	VBG
cord-277909-rn1dow26	27	20	turn	turn	NN
cord-277909-rn1dow26	27	21	around	around	RP
cord-277909-rn1dow26	27	22	times	time	NNS
cord-277909-rn1dow26	27	23	.	.	.
cord-277909-rn1dow26	28	1	The	the	DT
cord-277909-rn1dow26	28	2	issues	issue	NNS
cord-277909-rn1dow26	28	3	resulting	result	VBG
cord-277909-rn1dow26	28	4	from	from	IN
cord-277909-rn1dow26	28	5	this	this	DT
cord-277909-rn1dow26	28	6	experience	experience	NN
cord-277909-rn1dow26	28	7	(	(	-LRB-
cord-277909-rn1dow26	28	8	some	some	DT
cord-277909-rn1dow26	28	9	of	of	IN
cord-277909-rn1dow26	28	10	it	-PRON-	PRP
cord-277909-rn1dow26	28	11	bad	bad	JJ
cord-277909-rn1dow26	28	12	)	)	-RRB-
cord-277909-rn1dow26	28	13	are	be	VBP
cord-277909-rn1dow26	28	14	discussed	discuss	VBN
cord-277909-rn1dow26	28	15	in	in	IN
cord-277909-rn1dow26	28	16	detail	detail	NN
cord-277909-rn1dow26	28	17	below	below	IN
cord-277909-rn1dow26	28	18	with	with	IN
cord-277909-rn1dow26	28	19	the	the	DT
cord-277909-rn1dow26	28	20	aim	aim	NN
cord-277909-rn1dow26	28	21	of	of	IN
cord-277909-rn1dow26	28	22	informing	inform	VBG
cord-277909-rn1dow26	28	23	labo	labo	NNP
cord-277909-rn1dow26	28	24	-	-	HYPH
cord-277909-rn1dow26	28	25	ratories	ratories	NNP
cord-277909-rn1dow26	28	26	that	that	WDT
cord-277909-rn1dow26	28	27	are	be	VBP
cord-277909-rn1dow26	28	28	only	only	RB
cord-277909-rn1dow26	28	29	just	just	RB
cord-277909-rn1dow26	28	30	beginning	begin	VBG
cord-277909-rn1dow26	28	31	to	to	TO
cord-277909-rn1dow26	28	32	investigate	investigate	VB
cord-277909-rn1dow26	28	33	the	the	DT
cord-277909-rn1dow26	28	34	potential	potential	NN
cord-277909-rn1dow26	28	35	of	of	IN
cord-277909-rn1dow26	28	36	this	this	DT
cord-277909-rn1dow26	28	37	technology	technology	NN
cord-277909-rn1dow26	28	38	.	.	.
cord-277909-rn1dow26	29	1	There	there	EX
cord-277909-rn1dow26	29	2	are	be	VBP
cord-277909-rn1dow26	29	3	numerous	numerous	JJ
cord-277909-rn1dow26	29	4	chemistries	chemistry	NNS
cord-277909-rn1dow26	29	5	available	available	JJ
cord-277909-rn1dow26	29	6	to	to	TO
cord-277909-rn1dow26	29	7	carry	carry	VB
cord-277909-rn1dow26	29	8	out	out	RP
cord-277909-rn1dow26	29	9	real	real	JJ
cord-277909-rn1dow26	29	10	time	time	NN
cord-277909-rn1dow26	29	11	PCR	PCR	NNP
cord-277909-rn1dow26	29	12	.	.	.
cord-277909-rn1dow26	30	1	These	these	DT
cord-277909-rn1dow26	30	2	include	include	VBP
cord-277909-rn1dow26	30	3	dual	dual	JJ
cord-277909-rn1dow26	30	4	labelled	label	VBN
cord-277909-rn1dow26	30	5	probes	probe	NNS
cord-277909-rn1dow26	30	6	(	(	-LRB-
cord-277909-rn1dow26	30	7	often	often	RB
cord-277909-rn1dow26	30	8	known	know	VBN
cord-277909-rn1dow26	30	9	as	as	IN
cord-277909-rn1dow26	30	10	TaqMan	TaqMan	NNP
cord-277909-rn1dow26	30	11	TM	TM	NNP
cord-277909-rn1dow26	30	12	probes	probe	NNS
cord-277909-rn1dow26	30	13	)	)	-RRB-
cord-277909-rn1dow26	30	14	,	,	,
cord-277909-rn1dow26	30	15	minor	minor	JJ
cord-277909-rn1dow26	30	16	groove	groove	NN
cord-277909-rn1dow26	30	17	binding	bind	VBG
cord-277909-rn1dow26	30	18	(	(	-LRB-
cord-277909-rn1dow26	30	19	MGB	MGB	NNP
cord-277909-rn1dow26	30	20	)	)	-RRB-
cord-277909-rn1dow26	30	21	probes	probe	NNS
cord-277909-rn1dow26	30	22	,	,	,
cord-277909-rn1dow26	30	23	molecular	molecular	JJ
cord-277909-rn1dow26	30	24	beacons	beacon	NNS
cord-277909-rn1dow26	30	25	,	,	,
cord-277909-rn1dow26	30	26	fluorescence	fluorescence	NN
cord-277909-rn1dow26	30	27	energy	energy	NN
cord-277909-rn1dow26	30	28	transfer	transfer	NN
cord-277909-rn1dow26	30	29	(	(	-LRB-
cord-277909-rn1dow26	30	30	FRET	fret	NN
cord-277909-rn1dow26	30	31	)	)	-RRB-
cord-277909-rn1dow26	30	32	probes	probe	NNS
cord-277909-rn1dow26	30	33	,	,	,
cord-277909-rn1dow26	30	34	intercalating	intercalating	NN
cord-277909-rn1dow26	30	35	dyes	dye	NNS
cord-277909-rn1dow26	30	36	(	(	-LRB-
cord-277909-rn1dow26	30	37	such	such	JJ
cord-277909-rn1dow26	30	38	as	as	IN
cord-277909-rn1dow26	30	39	SYBR	SYBR	NNP
cord-277909-rn1dow26	30	40	green	green	NNP
cord-277909-rn1dow26	30	41	)	)	-RRB-
cord-277909-rn1dow26	30	42	and	and	CC
cord-277909-rn1dow26	30	43	more	more	RBR
cord-277909-rn1dow26	30	44	recently	recently	RB
cord-277909-rn1dow26	30	45	developed	develop	VBN
cord-277909-rn1dow26	30	46	fluorescent	fluorescent	NN
cord-277909-rn1dow26	30	47	labelled	label	VBN
cord-277909-rn1dow26	30	48	primers	primer	NNS
cord-277909-rn1dow26	30	49	such	such	JJ
cord-277909-rn1dow26	30	50	as	as	IN
cord-277909-rn1dow26	30	51	Sunrise	Sunrise	NNP
cord-277909-rn1dow26	30	52	TM	TM	NNP
cord-277909-rn1dow26	30	53	,	,	,
cord-277909-rn1dow26	30	54	Lux	Lux	NNP
cord-277909-rn1dow26	30	55	TM	TM	NNP
cord-277909-rn1dow26	30	56	or	or	CC
cord-277909-rn1dow26	30	57	Scorpion	Scorpion	NNP
cord-277909-rn1dow26	30	58	primers	primer	NNS
cord-277909-rn1dow26	30	59	TM	TM	NNP
cord-277909-rn1dow26	30	60	.	.	.
cord-277909-rn1dow26	31	1	The	the	DT
cord-277909-rn1dow26	31	2	advantages	advantage	NNS
cord-277909-rn1dow26	31	3	and	and	CC
cord-277909-rn1dow26	31	4	disadvantages	disadvantage	NNS
cord-277909-rn1dow26	31	5	of	of	IN
cord-277909-rn1dow26	31	6	each	each	DT
cord-277909-rn1dow26	31	7	chemistry	chemistry	NN
cord-277909-rn1dow26	31	8	are	be	VBP
cord-277909-rn1dow26	31	9	discussed	discuss	VBN
cord-277909-rn1dow26	31	10	(	(	-LRB-
cord-277909-rn1dow26	32	1	Arya	Arya	NNP
cord-277909-rn1dow26	32	2	et	et	NNP
cord-277909-rn1dow26	32	3	al	al	NNP
cord-277909-rn1dow26	32	4	.	.	NNP
cord-277909-rn1dow26	32	5	,	,	,
cord-277909-rn1dow26	32	6	2005	2005	CD
cord-277909-rn1dow26	32	7	;	;	:
cord-277909-rn1dow26	32	8	Aslanzadeh	Aslanzadeh	NNP
cord-277909-rn1dow26	32	9	,	,	,
cord-277909-rn1dow26	32	10	2004	2004	CD
cord-277909-rn1dow26	32	11	;	;	:
cord-277909-rn1dow26	32	12	Bustin	Bustin	NNP
cord-277909-rn1dow26	32	13	and	and	CC
cord-277909-rn1dow26	32	14	Nolan	Nolan	NNP
cord-277909-rn1dow26	32	15	,	,	,
cord-277909-rn1dow26	32	16	2004	2004	CD
cord-277909-rn1dow26	32	17	;	;	:
cord-277909-rn1dow26	32	18	Mackay	Mackay	NNP
cord-277909-rn1dow26	32	19	,	,	,
cord-277909-rn1dow26	32	20	2004	2004	CD
cord-277909-rn1dow26	32	21	;	;	:
cord-277909-rn1dow26	32	22	Tan	Tan	NNP
cord-277909-rn1dow26	32	23	et	et	FW
cord-277909-rn1dow26	32	24	al	al	NNP
cord-277909-rn1dow26	32	25	.	.	NNP
cord-277909-rn1dow26	32	26	,	,	,
cord-277909-rn1dow26	32	27	2004	2004	CD
cord-277909-rn1dow26	32	28	)	)	-RRB-
cord-277909-rn1dow26	32	29	(	(	-LRB-
cord-277909-rn1dow26	32	30	Table	table	NN
cord-277909-rn1dow26	32	31	1	1	CD
cord-277909-rn1dow26	32	32	)	)	-RRB-
cord-277909-rn1dow26	32	33	.	.	.
cord-277909-rn1dow26	33	1	Most	Most	JJS
cord-277909-rn1dow26	33	2	of	of	IN
cord-277909-rn1dow26	33	3	the	the	DT
cord-277909-rn1dow26	33	4	published	publish	VBN
cord-277909-rn1dow26	33	5	real	real	JJ
cord-277909-rn1dow26	33	6	time	time	NN
cord-277909-rn1dow26	33	7	probe	probe	NN
cord-277909-rn1dow26	33	8	based	base	VBN
cord-277909-rn1dow26	33	9	PCR	PCR	NNP
cord-277909-rn1dow26	33	10	assays	assay	NNS
cord-277909-rn1dow26	33	11	for	for	IN
cord-277909-rn1dow26	33	12	viral	viral	JJ
cord-277909-rn1dow26	33	13	diagnosis	diagnosis	NN
cord-277909-rn1dow26	33	14	utilise	utilise	VBP
cord-277909-rn1dow26	33	15	either	either	DT
cord-277909-rn1dow26	33	16	molecular	molecular	JJ
cord-277909-rn1dow26	33	17	beacons	beacon	NNS
cord-277909-rn1dow26	33	18	or	or	CC
cord-277909-rn1dow26	33	19	dual	dual	JJ
cord-277909-rn1dow26	33	20	labelled	label	VBN
cord-277909-rn1dow26	33	21	probes	probe	NNS
cord-277909-rn1dow26	33	22	although	although	IN
cord-277909-rn1dow26	33	23	more	more	JJR
cord-277909-rn1dow26	33	24	recent	recent	JJ
cord-277909-rn1dow26	33	25	publications	publication	NNS
cord-277909-rn1dow26	33	26	tend	tend	VBP
cord-277909-rn1dow26	33	27	to	to	TO
cord-277909-rn1dow26	33	28	favour	favour	VB
cord-277909-rn1dow26	33	29	the	the	DT
cord-277909-rn1dow26	33	30	use	use	NN
cord-277909-rn1dow26	33	31	of	of	IN
cord-277909-rn1dow26	33	32	dual	dual	JJ
cord-277909-rn1dow26	33	33	labelled	label	VBN
cord-277909-rn1dow26	33	34	probes	probe	NNS
cord-277909-rn1dow26	33	35	.	.	.
cord-277909-rn1dow26	34	1	Currently	currently	RB
cord-277909-rn1dow26	34	2	all	all	DT
cord-277909-rn1dow26	34	3	our	-PRON-	PRP$
cord-277909-rn1dow26	34	4	real	real	JJ
cord-277909-rn1dow26	34	5	time	time	NN
cord-277909-rn1dow26	34	6	PCR	PCR	NNP
cord-277909-rn1dow26	34	7	tests	test	NNS
cord-277909-rn1dow26	34	8	are	be	VBP
cord-277909-rn1dow26	34	9	dual	dual	JJ
cord-277909-rn1dow26	34	10	labelled	label	VBN
cord-277909-rn1dow26	34	11	probe	probe	NN
cord-277909-rn1dow26	34	12	assays	assay	NNS
cord-277909-rn1dow26	34	13	.	.	.
cord-277909-rn1dow26	35	1	However	however	RB
cord-277909-rn1dow26	35	2	,	,	,
cord-277909-rn1dow26	35	3	we	-PRON-	PRP
cord-277909-rn1dow26	35	4	do	do	VBP
cord-277909-rn1dow26	35	5	have	have	VB
cord-277909-rn1dow26	35	6	experience	experience	NN
cord-277909-rn1dow26	35	7	of	of	IN
cord-277909-rn1dow26	35	8	both	both	DT
cord-277909-rn1dow26	35	9	methods	method	NNS
cord-277909-rn1dow26	35	10	and	and	CC
cord-277909-rn1dow26	35	11	have	have	VBP
cord-277909-rn1dow26	35	12	noted	note	VBN
cord-277909-rn1dow26	35	13	that	that	IN
cord-277909-rn1dow26	35	14	there	there	EX
cord-277909-rn1dow26	35	15	are	be	VBP
cord-277909-rn1dow26	35	16	several	several	JJ
cord-277909-rn1dow26	35	17	important	important	JJ
cord-277909-rn1dow26	35	18	differences	difference	NNS
cord-277909-rn1dow26	35	19	between	between	IN
cord-277909-rn1dow26	35	20	these	these	DT
cord-277909-rn1dow26	35	21	two	two	CD
cord-277909-rn1dow26	35	22	systems	system	NNS
cord-277909-rn1dow26	35	23	,	,	,
cord-277909-rn1dow26	35	24	which	which	WDT
cord-277909-rn1dow26	35	25	should	should	MD
cord-277909-rn1dow26	35	26	be	be	VB
cord-277909-rn1dow26	35	27	considered	consider	VBN
cord-277909-rn1dow26	35	28	before	before	IN
cord-277909-rn1dow26	35	29	developing	develop	VBG
cord-277909-rn1dow26	35	30	or	or	CC
cord-277909-rn1dow26	35	31	implementing	implement	VBG
cord-277909-rn1dow26	35	32	a	a	DT
cord-277909-rn1dow26	35	33	diagnostic	diagnostic	JJ
cord-277909-rn1dow26	35	34	virology	virology	NN
cord-277909-rn1dow26	35	35	test	test	NN
cord-277909-rn1dow26	35	36	.	.	.
cord-277909-rn1dow26	36	1	Molecular	molecular	JJ
cord-277909-rn1dow26	36	2	beacons	beacon	NNS
cord-277909-rn1dow26	36	3	are	be	VBP
cord-277909-rn1dow26	36	4	very	very	RB
cord-277909-rn1dow26	36	5	specific	specific	JJ
cord-277909-rn1dow26	36	6	(	(	-LRB-
cord-277909-rn1dow26	36	7	Tyagi	Tyagi	NNP
cord-277909-rn1dow26	36	8	and	and	CC
cord-277909-rn1dow26	36	9	Kramer	Kramer	NNP
cord-277909-rn1dow26	36	10	,	,	,
cord-277909-rn1dow26	36	11	1996	1996	CD
cord-277909-rn1dow26	36	12	)	)	-RRB-
cord-277909-rn1dow26	36	13	.	.	.
cord-277909-rn1dow26	37	1	The	the	DT
cord-277909-rn1dow26	37	2	specificity	specificity	NN
cord-277909-rn1dow26	37	3	is	be	VBZ
cord-277909-rn1dow26	37	4	a	a	DT
cord-277909-rn1dow26	37	5	direct	direct	JJ
cord-277909-rn1dow26	37	6	result	result	NN
cord-277909-rn1dow26	37	7	of	of	IN
cord-277909-rn1dow26	37	8	their	-PRON-	PRP$
cord-277909-rn1dow26	37	9	structure	structure	NN
cord-277909-rn1dow26	37	10	.	.	.
cord-277909-rn1dow26	38	1	In	in	IN
cord-277909-rn1dow26	38	2	free	free	JJ
cord-277909-rn1dow26	38	3	solution	solution	NN
cord-277909-rn1dow26	38	4	a	a	DT
cord-277909-rn1dow26	38	5	molecular	molecular	JJ
cord-277909-rn1dow26	38	6	beacon	beacon	NN
cord-277909-rn1dow26	38	7	adopts	adopt	VBZ
cord-277909-rn1dow26	38	8	a	a	DT
cord-277909-rn1dow26	38	9	hairpin	hairpin	JJ
cord-277909-rn1dow26	38	10	-	-	HYPH
cord-277909-rn1dow26	38	11	loop	loop	NN
cord-277909-rn1dow26	38	12	conformation	conformation	NN
cord-277909-rn1dow26	38	13	in	in	IN
cord-277909-rn1dow26	38	14	which	which	WDT
cord-277909-rn1dow26	38	15	the	the	DT
cord-277909-rn1dow26	38	16	reporter	reporter	NN
cord-277909-rn1dow26	38	17	fluorescence	fluorescence	NN
cord-277909-rn1dow26	38	18	is	be	VBZ
cord-277909-rn1dow26	38	19	quenched	quench	VBN
cord-277909-rn1dow26	38	20	by	by	IN
cord-277909-rn1dow26	38	21	its	-PRON-	PRP$
cord-277909-rn1dow26	38	22	proximity	proximity	NN
cord-277909-rn1dow26	38	23	to	to	IN
cord-277909-rn1dow26	38	24	the	the	DT
cord-277909-rn1dow26	38	25	quencher	quencher	NN
cord-277909-rn1dow26	38	26	molecule	molecule	NN
cord-277909-rn1dow26	39	1	(	(	-LRB-
cord-277909-rn1dow26	39	2	Fig	Fig	NNP
cord-277909-rn1dow26	39	3	.	.	NNP
cord-277909-rn1dow26	39	4	1	1	CD
cord-277909-rn1dow26	39	5	)	)	-RRB-
cord-277909-rn1dow26	39	6	.	.	.
cord-277909-rn1dow26	40	1	This	this	DT
cord-277909-rn1dow26	40	2	is	be	VBZ
cord-277909-rn1dow26	40	3	a	a	DT
cord-277909-rn1dow26	40	4	very	very	RB
cord-277909-rn1dow26	40	5	stable	stable	JJ
cord-277909-rn1dow26	40	6	state	state	NN
cord-277909-rn1dow26	40	7	and	and	CC
cord-277909-rn1dow26	40	8	a	a	DT
cord-277909-rn1dow26	40	9	molecular	molecular	JJ
cord-277909-rn1dow26	40	10	beacon	beacon	NN
cord-277909-rn1dow26	40	11	will	will	MD
cord-277909-rn1dow26	40	12	only	only	RB
cord-277909-rn1dow26	40	13	bind	bind	VB
cord-277909-rn1dow26	40	14	to	to	IN
cord-277909-rn1dow26	40	15	a	a	DT
cord-277909-rn1dow26	40	16	target	target	NN
cord-277909-rn1dow26	40	17	sequence	sequence	NN
cord-277909-rn1dow26	40	18	,	,	,
cord-277909-rn1dow26	40	19	become	become	VB
cord-277909-rn1dow26	40	20	linear	linear	JJ
cord-277909-rn1dow26	40	21	and	and	CC
cord-277909-rn1dow26	40	22	fluoresce	fluoresce	VB
cord-277909-rn1dow26	40	23	if	if	IN
cord-277909-rn1dow26	40	24	it	-PRON-	PRP
cord-277909-rn1dow26	40	25	is	be	VBZ
cord-277909-rn1dow26	40	26	highly	highly	RB
cord-277909-rn1dow26	40	27	complementary	complementary	JJ
cord-277909-rn1dow26	40	28	.	.	.
cord-277909-rn1dow26	41	1	Any	any	DT
cord-277909-rn1dow26	41	2	nucleotide	nucleotide	JJ
cord-277909-rn1dow26	41	3	differences	difference	NNS
cord-277909-rn1dow26	41	4	between	between	IN
cord-277909-rn1dow26	41	5	the	the	DT
cord-277909-rn1dow26	41	6	beacon	beacon	NN
cord-277909-rn1dow26	41	7	and	and	CC
cord-277909-rn1dow26	41	8	the	the	DT
cord-277909-rn1dow26	41	9	target	target	NN
cord-277909-rn1dow26	41	10	sequence	sequence	NN
cord-277909-rn1dow26	41	11	will	will	MD
cord-277909-rn1dow26	41	12	greatly	greatly	RB
cord-277909-rn1dow26	41	13	reduce	reduce	VB
cord-277909-rn1dow26	41	14	the	the	DT
cord-277909-rn1dow26	41	15	target	target	NN
cord-277909-rn1dow26	41	16	binding	bind	VBG
cord-277909-rn1dow26	41	17	efficiency	efficiency	NN
cord-277909-rn1dow26	41	18	of	of	IN
cord-277909-rn1dow26	41	19	the	the	DT
cord-277909-rn1dow26	41	20	probe	probe	NN
cord-277909-rn1dow26	41	21	.	.	.
cord-277909-rn1dow26	42	1	As	as	IN
cord-277909-rn1dow26	42	2	a	a	DT
cord-277909-rn1dow26	42	3	result	result	NN
cord-277909-rn1dow26	42	4	molecular	molecular	JJ
cord-277909-rn1dow26	42	5	beacons	beacon	NNS
cord-277909-rn1dow26	42	6	have	have	VBP
cord-277909-rn1dow26	42	7	an	an	DT
cord-277909-rn1dow26	42	8	increased	increase	VBN
cord-277909-rn1dow26	42	9	propensity	propensity	NN
cord-277909-rn1dow26	42	10	for	for	IN
cord-277909-rn1dow26	42	11	false	false	JJ
cord-277909-rn1dow26	42	12	negative	negative	JJ
cord-277909-rn1dow26	42	13	results	result	NNS
cord-277909-rn1dow26	42	14	.	.	.
cord-277909-rn1dow26	43	1	We	-PRON-	PRP
cord-277909-rn1dow26	43	2	encountered	encounter	VBD
cord-277909-rn1dow26	43	3	this	this	DT
cord-277909-rn1dow26	43	4	problem	problem	NN
cord-277909-rn1dow26	43	5	during	during	IN
cord-277909-rn1dow26	43	6	the	the	DT
cord-277909-rn1dow26	43	7	implementation	implementation	NN
cord-277909-rn1dow26	43	8	of	of	IN
cord-277909-rn1dow26	43	9	a	a	DT
cord-277909-rn1dow26	43	10	previously	previously	RB
cord-277909-rn1dow26	43	11	published	publish	VBN
cord-277909-rn1dow26	43	12	molecular	molecular	JJ
cord-277909-rn1dow26	43	13	beacon	beacon	NN
cord-277909-rn1dow26	43	14	based	base	VBN
cord-277909-rn1dow26	43	15	test	test	NN
cord-277909-rn1dow26	43	16	for	for	IN
cord-277909-rn1dow26	43	17	the	the	DT
cord-277909-rn1dow26	43	18	detection	detection	NN
cord-277909-rn1dow26	43	19	of	of	IN
cord-277909-rn1dow26	43	20	parainfluenza	parainfluenza	NNP
cord-277909-rn1dow26	43	21	viruses	virus	NNS
cord-277909-rn1dow26	43	22	.	.	.
cord-277909-rn1dow26	44	1	During	during	IN
cord-277909-rn1dow26	44	2	the	the	DT
cord-277909-rn1dow26	44	3	initial	initial	JJ
cord-277909-rn1dow26	44	4	assessment	assessment	NN
cord-277909-rn1dow26	44	5	,	,	,
cord-277909-rn1dow26	44	6	this	this	DT
cord-277909-rn1dow26	44	7	detected	detect	VBD
cord-277909-rn1dow26	44	8	all	all	DT
cord-277909-rn1dow26	44	9	culture	culture	NN
cord-277909-rn1dow26	44	10	/	/	SYM
cord-277909-rn1dow26	44	11	direct	direct	NNP
cord-277909-rn1dow26	44	12	immunoflorescence	immunoflorescence	NNP
cord-277909-rn1dow26	44	13	parainfluenza	parainfluenza	NNP
cord-277909-rn1dow26	44	14	3	3	CD
cord-277909-rn1dow26	44	15	positive	positive	JJ
cord-277909-rn1dow26	44	16	samples	sample	NNS
cord-277909-rn1dow26	44	17	detected	detect	VBN
cord-277909-rn1dow26	44	18	between	between	IN
cord-277909-rn1dow26	44	19	2001	2001	CD
cord-277909-rn1dow26	44	20	and	and	CC
cord-277909-rn1dow26	44	21	2002	2002	CD
cord-277909-rn1dow26	44	22	.	.	.
cord-277909-rn1dow26	45	1	However	however	RB
cord-277909-rn1dow26	45	2	,	,	,
cord-277909-rn1dow26	45	3	all	all	DT
cord-277909-rn1dow26	45	4	parainfluenza	parainfluenza	VBP
cord-277909-rn1dow26	45	5	3	3	CD
cord-277909-rn1dow26	45	6	positive	positive	JJ
cord-277909-rn1dow26	45	7	samples	sample	NNS
cord-277909-rn1dow26	45	8	detected	detect	VBN
cord-277909-rn1dow26	45	9	by	by	IN
cord-277909-rn1dow26	45	10	DIF	DIF	NNP
cord-277909-rn1dow26	45	11	or	or	CC
cord-277909-rn1dow26	45	12	culture	culture	NN
cord-277909-rn1dow26	45	13	in	in	IN
cord-277909-rn1dow26	45	14	2003	2003	CD
cord-277909-rn1dow26	45	15	were	be	VBD
cord-277909-rn1dow26	45	16	negative	negative	JJ
cord-277909-rn1dow26	45	17	when	when	WRB
cord-277909-rn1dow26	45	18	tested	test	VBN
cord-277909-rn1dow26	45	19	with	with	IN
cord-277909-rn1dow26	45	20	this	this	DT
cord-277909-rn1dow26	45	21	assay	assay	NN
cord-277909-rn1dow26	45	22	.	.	.
cord-277909-rn1dow26	46	1	To	to	TO
cord-277909-rn1dow26	46	2	assess	assess	VB
cord-277909-rn1dow26	46	3	whether	whether	IN
cord-277909-rn1dow26	46	4	the	the	DT
cord-277909-rn1dow26	46	5	primers	primer	NNS
cord-277909-rn1dow26	46	6	were	be	VBD
cord-277909-rn1dow26	46	7	amplifying	amplify	VBG
cord-277909-rn1dow26	46	8	the	the	DT
cord-277909-rn1dow26	46	9	parainfluenza	parainfluenza	JJ
cord-277909-rn1dow26	46	10	3	3	CD
cord-277909-rn1dow26	46	11	viral	viral	JJ
cord-277909-rn1dow26	46	12	RNA	RNA	NNP
cord-277909-rn1dow26	46	13	,	,	,
cord-277909-rn1dow26	46	14	SYBR	SYBR	NNP
cord-277909-rn1dow26	46	15	green	green	NNP
cord-277909-rn1dow26	46	16	was	be	VBD
cord-277909-rn1dow26	46	17	added	add	VBN
cord-277909-rn1dow26	46	18	to	to	IN
cord-277909-rn1dow26	46	19	the	the	DT
cord-277909-rn1dow26	46	20	PCR	PCR	NNP
cord-277909-rn1dow26	46	21	reaction	reaction	NN
cord-277909-rn1dow26	46	22	in	in	IN
cord-277909-rn1dow26	46	23	place	place	NN
cord-277909-rn1dow26	46	24	of	of	IN
cord-277909-rn1dow26	46	25	the	the	DT
cord-277909-rn1dow26	46	26	molecular	molecular	JJ
cord-277909-rn1dow26	46	27	beacon	beacon	NN
cord-277909-rn1dow26	46	28	.	.	.
cord-277909-rn1dow26	47	1	The	the	DT
cord-277909-rn1dow26	47	2	formation	formation	NN
cord-277909-rn1dow26	47	3	of	of	IN
cord-277909-rn1dow26	47	4	PCR	PCR	NNP
cord-277909-rn1dow26	47	5	product	product	NN
cord-277909-rn1dow26	47	6	was	be	VBD
cord-277909-rn1dow26	47	7	observed	observe	VBN
cord-277909-rn1dow26	47	8	.	.	.
cord-277909-rn1dow26	48	1	Using	use	VBG
cord-277909-rn1dow26	48	2	melt	melt	NN
cord-277909-rn1dow26	48	3	curve	curve	NN
cord-277909-rn1dow26	48	4	analysis	analysis	NN
cord-277909-rn1dow26	48	5	,	,	,
cord-277909-rn1dow26	48	6	identical	identical	JJ
cord-277909-rn1dow26	48	7	melting	melt	VBG
cord-277909-rn1dow26	48	8	peaks	peak	NNS
cord-277909-rn1dow26	48	9	were	be	VBD
cord-277909-rn1dow26	48	10	observed	observe	VBN
cord-277909-rn1dow26	48	11	in	in	IN
cord-277909-rn1dow26	48	12	all	all	DT
cord-277909-rn1dow26	48	13	parainfluenza	parainfluenza	JJ
cord-277909-rn1dow26	48	14	3	3	CD
cord-277909-rn1dow26	48	15	samples	sample	NNS
cord-277909-rn1dow26	48	16	and	and	CC
cord-277909-rn1dow26	48	17	controls	control	NNS
cord-277909-rn1dow26	48	18	(	(	-LRB-
cord-277909-rn1dow26	48	19	Fig	Fig	NNP
cord-277909-rn1dow26	48	20	.	.	NNP
cord-277909-rn1dow26	48	21	2	2	CD
cord-277909-rn1dow26	48	22	)	)	-RRB-
cord-277909-rn1dow26	48	23	.	.	.
cord-277909-rn1dow26	49	1	Running	run	VBG
cord-277909-rn1dow26	49	2	the	the	DT
cord-277909-rn1dow26	49	3	PCR	PCR	NNP
cord-277909-rn1dow26	49	4	products	product	NNS
cord-277909-rn1dow26	49	5	on	on	IN
cord-277909-rn1dow26	49	6	an	an	DT
cord-277909-rn1dow26	49	7	agarose	agarose	JJ
cord-277909-rn1dow26	49	8	gel	gel	NN
cord-277909-rn1dow26	49	9	and	and	CC
cord-277909-rn1dow26	49	10	observing	observe	VBG
cord-277909-rn1dow26	49	11	a	a	DT
cord-277909-rn1dow26	49	12	band	band	NN
cord-277909-rn1dow26	49	13	of	of	IN
cord-277909-rn1dow26	49	14	the	the	DT
cord-277909-rn1dow26	49	15	expected	expect	VBN
cord-277909-rn1dow26	49	16	size	size	NN
cord-277909-rn1dow26	49	17	confirmed	confirm	VBD
cord-277909-rn1dow26	49	18	the	the	DT
cord-277909-rn1dow26	49	19	successful	successful	JJ
cord-277909-rn1dow26	49	20	amplification	amplification	NN
cord-277909-rn1dow26	49	21	of	of	IN
cord-277909-rn1dow26	49	22	parainfluenza	parainfluenza	NNP
cord-277909-rn1dow26	49	23	3	3	CD
cord-277909-rn1dow26	49	24	RNA	RNA	NNP
cord-277909-rn1dow26	49	25	by	by	IN
cord-277909-rn1dow26	49	26	the	the	DT
cord-277909-rn1dow26	49	27	primers	primer	NNS
cord-277909-rn1dow26	49	28	.	.	.
cord-277909-rn1dow26	50	1	Based	base	VBN
cord-277909-rn1dow26	50	2	on	on	IN
cord-277909-rn1dow26	50	3	these	these	DT
cord-277909-rn1dow26	50	4	results	result	NNS
cord-277909-rn1dow26	50	5	we	-PRON-	PRP
cord-277909-rn1dow26	50	6	deduced	deduce	VBD
cord-277909-rn1dow26	50	7	that	that	IN
cord-277909-rn1dow26	50	8	the	the	DT
cord-277909-rn1dow26	50	9	molecular	molecular	JJ
cord-277909-rn1dow26	50	10	beacon	beacon	NN
cord-277909-rn1dow26	50	11	was	be	VBD
cord-277909-rn1dow26	50	12	no	no	RB
cord-277909-rn1dow26	50	13	longer	long	RBR
cord-277909-rn1dow26	50	14	complementary	complementary	JJ
cord-277909-rn1dow26	50	15	to	to	IN
cord-277909-rn1dow26	50	16	the	the	DT
cord-277909-rn1dow26	50	17	amplified	amplify	VBN
cord-277909-rn1dow26	50	18	target	target	NN
cord-277909-rn1dow26	50	19	sequence	sequence	NN
cord-277909-rn1dow26	50	20	.	.	.
cord-277909-rn1dow26	51	1	Consequently	consequently	RB
cord-277909-rn1dow26	51	2	,	,	,
cord-277909-rn1dow26	51	3	following	follow	VBG
cord-277909-rn1dow26	51	4	analysis	analysis	NN
cord-277909-rn1dow26	51	5	of	of	IN
cord-277909-rn1dow26	51	6	more	more	RBR
cord-277909-rn1dow26	51	7	recently	recently	RB
cord-277909-rn1dow26	51	8	available	available	JJ
cord-277909-rn1dow26	51	9	sequences	sequence	NNS
cord-277909-rn1dow26	51	10	in	in	IN
cord-277909-rn1dow26	51	11	the	the	DT
cord-277909-rn1dow26	51	12	database	database	NN
cord-277909-rn1dow26	51	13	,	,	,
cord-277909-rn1dow26	51	14	a	a	DT
cord-277909-rn1dow26	51	15	new	new	JJ
cord-277909-rn1dow26	51	16	molecular	molecular	JJ
cord-277909-rn1dow26	51	17	beacon	beacon	NN
cord-277909-rn1dow26	51	18	was	be	VBD
cord-277909-rn1dow26	51	19	designed	design	VBN
cord-277909-rn1dow26	51	20	,	,	,
cord-277909-rn1dow26	51	21	which	which	WDT
cord-277909-rn1dow26	51	22	detected	detect	VBD
cord-277909-rn1dow26	51	23	all	all	DT
cord-277909-rn1dow26	51	24	2003	2003	CD
cord-277909-rn1dow26	51	25	parainfluenza	parainfluenza	NNP
cord-277909-rn1dow26	51	26	3	3	CD
cord-277909-rn1dow26	51	27	samples	sample	NNS
cord-277909-rn1dow26	51	28	.	.	.
cord-277909-rn1dow26	52	1	Fig	fig	NN
cord-277909-rn1dow26	53	1	Unlike	unlike	IN
cord-277909-rn1dow26	53	2	molecular	molecular	JJ
cord-277909-rn1dow26	53	3	beacons	beacon	NNS
cord-277909-rn1dow26	53	4	,	,	,
cord-277909-rn1dow26	53	5	dual	dual	JJ
cord-277909-rn1dow26	53	6	labelled	label	VBN
cord-277909-rn1dow26	53	7	probes	probe	NNS
cord-277909-rn1dow26	53	8	are	be	VBP
cord-277909-rn1dow26	53	9	in	in	IN
cord-277909-rn1dow26	53	10	a	a	DT
cord-277909-rn1dow26	53	11	permanent	permanent	JJ
cord-277909-rn1dow26	53	12	linear	linear	JJ
cord-277909-rn1dow26	53	13	conformation	conformation	NN
cord-277909-rn1dow26	54	1	(	(	-LRB-
cord-277909-rn1dow26	54	2	Lee	Lee	NNP
cord-277909-rn1dow26	54	3	et	et	NNP
cord-277909-rn1dow26	54	4	al	al	NNP
cord-277909-rn1dow26	54	5	.	.	NNP
cord-277909-rn1dow26	54	6	,	,	,
cord-277909-rn1dow26	54	7	1993	1993	CD
cord-277909-rn1dow26	54	8	)	)	-RRB-
cord-277909-rn1dow26	55	1	(	(	-LRB-
cord-277909-rn1dow26	55	2	Fig	Fig	NNP
cord-277909-rn1dow26	55	3	.	.	.
cord-277909-rn1dow26	55	4	3	3	CD
cord-277909-rn1dow26	55	5	)	)	-RRB-
cord-277909-rn1dow26	55	6	.	.	.
cord-277909-rn1dow26	56	1	Like	like	IN
cord-277909-rn1dow26	56	2	primers	primer	NNS
cord-277909-rn1dow26	56	3	,	,	,
cord-277909-rn1dow26	56	4	they	-PRON-	PRP
cord-277909-rn1dow26	56	5	can	can	MD
cord-277909-rn1dow26	56	6	tolerate	tolerate	VB
cord-277909-rn1dow26	56	7	a	a	DT
cord-277909-rn1dow26	56	8	small	small	JJ
cord-277909-rn1dow26	56	9	number	number	NN
cord-277909-rn1dow26	56	10	of	of	IN
cord-277909-rn1dow26	56	11	mismatches	mismatch	NNS
cord-277909-rn1dow26	56	12	between	between	IN
cord-277909-rn1dow26	56	13	the	the	DT
cord-277909-rn1dow26	56	14	probe	probe	NN
cord-277909-rn1dow26	56	15	and	and	CC
cord-277909-rn1dow26	56	16	target	target	NN
cord-277909-rn1dow26	56	17	and	and	CC
cord-277909-rn1dow26	56	18	still	still	RB
cord-277909-rn1dow26	56	19	bind	bind	VB
cord-277909-rn1dow26	56	20	to	to	IN
cord-277909-rn1dow26	56	21	the	the	DT
cord-277909-rn1dow26	56	22	target	target	NN
cord-277909-rn1dow26	56	23	.	.	.
cord-277909-rn1dow26	57	1	Consequently	consequently	RB
cord-277909-rn1dow26	57	2	,	,	,
cord-277909-rn1dow26	57	3	dual	dual	JJ
cord-277909-rn1dow26	57	4	labelled	label	VBN
cord-277909-rn1dow26	57	5	probes	probe	NNS
cord-277909-rn1dow26	57	6	are	be	VBP
cord-277909-rn1dow26	57	7	less	less	RBR
cord-277909-rn1dow26	57	8	likely	likely	JJ
cord-277909-rn1dow26	57	9	to	to	TO
cord-277909-rn1dow26	57	10	result	result	VB
cord-277909-rn1dow26	57	11	in	in	IN
cord-277909-rn1dow26	57	12	false	false	JJ
cord-277909-rn1dow26	57	13	negative	negative	JJ
cord-277909-rn1dow26	57	14	reactions	reaction	NNS
cord-277909-rn1dow26	57	15	and	and	CC
cord-277909-rn1dow26	57	16	may	may	MD
cord-277909-rn1dow26	57	17	,	,	,
cord-277909-rn1dow26	57	18	in	in	IN
cord-277909-rn1dow26	57	19	comparison	comparison	NN
cord-277909-rn1dow26	57	20	with	with	IN
cord-277909-rn1dow26	57	21	molecular	molecular	JJ
cord-277909-rn1dow26	57	22	beacons	beacon	NNS
cord-277909-rn1dow26	57	23	,	,	,
cord-277909-rn1dow26	57	24	be	be	VB
cord-277909-rn1dow26	57	25	of	of	IN
cord-277909-rn1dow26	57	26	greater	great	JJR
cord-277909-rn1dow26	57	27	use	use	NN
cord-277909-rn1dow26	57	28	in	in	IN
cord-277909-rn1dow26	57	29	viral	viral	JJ
cord-277909-rn1dow26	57	30	diagnosis	diagnosis	NN
cord-277909-rn1dow26	57	31	where	where	WRB
cord-277909-rn1dow26	57	32	occasional	occasional	JJ
cord-277909-rn1dow26	57	33	changes	change	NNS
cord-277909-rn1dow26	57	34	in	in	IN
cord-277909-rn1dow26	57	35	even	even	RB
cord-277909-rn1dow26	57	36	the	the	DT
cord-277909-rn1dow26	57	37	most	most	RBS
cord-277909-rn1dow26	57	38	conserved	conserved	JJ
cord-277909-rn1dow26	57	39	target	target	NN
cord-277909-rn1dow26	57	40	sequence	sequence	NN
cord-277909-rn1dow26	57	41	may	may	MD
cord-277909-rn1dow26	57	42	be	be	VB
cord-277909-rn1dow26	57	43	expected	expect	VBN
cord-277909-rn1dow26	57	44	to	to	TO
cord-277909-rn1dow26	57	45	occur	occur	VB
cord-277909-rn1dow26	57	46	(	(	-LRB-
cord-277909-rn1dow26	57	47	although	although	IN
cord-277909-rn1dow26	57	48	it	-PRON-	PRP
cord-277909-rn1dow26	57	49	should	should	MD
cord-277909-rn1dow26	57	50	be	be	VB
cord-277909-rn1dow26	57	51	noted	note	VBN
cord-277909-rn1dow26	57	52	that	that	IN
cord-277909-rn1dow26	57	53	mismatches	mismatch	NNS
cord-277909-rn1dow26	57	54	can	can	MD
cord-277909-rn1dow26	57	55	also	also	RB
cord-277909-rn1dow26	57	56	lead	lead	VB
cord-277909-rn1dow26	57	57	to	to	IN
cord-277909-rn1dow26	57	58	false	false	JJ
cord-277909-rn1dow26	57	59	negative	negative	JJ
cord-277909-rn1dow26	57	60	reactions	reaction	NNS
cord-277909-rn1dow26	57	61	with	with	IN
cord-277909-rn1dow26	57	62	dual	dual	JJ
cord-277909-rn1dow26	57	63	labelled	label	VBN
cord-277909-rn1dow26	57	64	probes	probe	NNS
cord-277909-rn1dow26	57	65	)	)	-RRB-
cord-277909-rn1dow26	57	66	.	.	.
cord-277909-rn1dow26	58	1	However	however	RB
cord-277909-rn1dow26	58	2	,	,	,
cord-277909-rn1dow26	58	3	either	either	DT
cord-277909-rn1dow26	58	4	method	method	NN
cord-277909-rn1dow26	58	5	will	will	MD
cord-277909-rn1dow26	58	6	be	be	VB
cord-277909-rn1dow26	58	7	useful	useful	JJ
cord-277909-rn1dow26	58	8	if	if	IN
cord-277909-rn1dow26	58	9	targeting	target	VBG
cord-277909-rn1dow26	58	10	a	a	DT
cord-277909-rn1dow26	58	11	highly	highly	RB
cord-277909-rn1dow26	58	12	conserved	conserve	VBN
cord-277909-rn1dow26	58	13	region	region	NN
cord-277909-rn1dow26	58	14	.	.	.
cord-277909-rn1dow26	59	1	The	the	DT
cord-277909-rn1dow26	59	2	second	second	JJ
cord-277909-rn1dow26	59	3	difference	difference	NN
cord-277909-rn1dow26	59	4	between	between	IN
cord-277909-rn1dow26	59	5	molecular	molecular	JJ
cord-277909-rn1dow26	59	6	beacons	beacon	NNS
cord-277909-rn1dow26	59	7	and	and	CC
cord-277909-rn1dow26	59	8	dual	dual	JJ
cord-277909-rn1dow26	59	9	labelled	label	VBN
cord-277909-rn1dow26	59	10	probe	probe	NN
cord-277909-rn1dow26	59	11	chemistries	chemistry	NNS
cord-277909-rn1dow26	59	12	is	be	VBZ
cord-277909-rn1dow26	59	13	related	relate	VBN
cord-277909-rn1dow26	59	14	to	to	IN
cord-277909-rn1dow26	59	15	the	the	DT
cord-277909-rn1dow26	59	16	normalised	normalised	JJ
cord-277909-rn1dow26	59	17	Fig	Fig	NNP
cord-277909-rn1dow26	59	18	.	.	.
cord-277909-rn1dow26	60	1	2	2	LS
cord-277909-rn1dow26	60	2	.	.	.
cord-277909-rn1dow26	61	1	Figure	figure	NN
cord-277909-rn1dow26	61	2	showing	show	VBG
cord-277909-rn1dow26	61	3	assessment	assessment	NN
cord-277909-rn1dow26	61	4	of	of	IN
cord-277909-rn1dow26	61	5	whether	whether	IN
cord-277909-rn1dow26	61	6	real	real	JJ
cord-277909-rn1dow26	61	7	time	time	NN
cord-277909-rn1dow26	61	8	molecular	molecular	JJ
cord-277909-rn1dow26	61	9	beacon	beacon	NN
cord-277909-rn1dow26	61	10	primers	primer	NNS
cord-277909-rn1dow26	61	11	were	be	VBD
cord-277909-rn1dow26	61	12	amplifying	amplify	VBG
cord-277909-rn1dow26	61	13	the	the	DT
cord-277909-rn1dow26	61	14	parainfluenza	parainfluenza	JJ
cord-277909-rn1dow26	61	15	3	3	CD
cord-277909-rn1dow26	61	16	viral	viral	JJ
cord-277909-rn1dow26	61	17	RNA	RNA	NNP
cord-277909-rn1dow26	61	18	.	.	.
cord-277909-rn1dow26	62	1	In	in	IN
cord-277909-rn1dow26	62	2	this	this	DT
cord-277909-rn1dow26	62	3	reaction	reaction	NN
cord-277909-rn1dow26	62	4	,	,	,
cord-277909-rn1dow26	62	5	SYBR	SYBR	NNP
cord-277909-rn1dow26	62	6	green	green	NNP
cord-277909-rn1dow26	62	7	was	be	VBD
cord-277909-rn1dow26	62	8	added	add	VBN
cord-277909-rn1dow26	62	9	to	to	IN
cord-277909-rn1dow26	62	10	the	the	DT
cord-277909-rn1dow26	62	11	PCR	PCR	NNP
cord-277909-rn1dow26	62	12	reaction	reaction	NN
cord-277909-rn1dow26	62	13	in	in	IN
cord-277909-rn1dow26	62	14	place	place	NN
cord-277909-rn1dow26	62	15	of	of	IN
cord-277909-rn1dow26	62	16	the	the	DT
cord-277909-rn1dow26	62	17	molecular	molecular	JJ
cord-277909-rn1dow26	62	18	beacon	beacon	NN
cord-277909-rn1dow26	62	19	(	(	-LRB-
cord-277909-rn1dow26	62	20	all	all	DT
cord-277909-rn1dow26	62	21	samples	sample	NNS
cord-277909-rn1dow26	62	22	negative	negative	JJ
cord-277909-rn1dow26	62	23	by	by	IN
cord-277909-rn1dow26	62	24	molecular	molecular	JJ
cord-277909-rn1dow26	62	25	beacon	beacon	NN
cord-277909-rn1dow26	62	26	assay	assay	NN
cord-277909-rn1dow26	62	27	)	)	-RRB-
cord-277909-rn1dow26	62	28	.	.	.
cord-277909-rn1dow26	63	1	The	the	DT
cord-277909-rn1dow26	63	2	formation	formation	NN
cord-277909-rn1dow26	63	3	of	of	IN
cord-277909-rn1dow26	63	4	PCR	PCR	NNP
cord-277909-rn1dow26	63	5	product	product	NN
cord-277909-rn1dow26	63	6	was	be	VBD
cord-277909-rn1dow26	63	7	observed	observe	VBN
cord-277909-rn1dow26	63	8	.	.	.
cord-277909-rn1dow26	64	1	Using	use	VBG
cord-277909-rn1dow26	64	2	melt	melt	NN
cord-277909-rn1dow26	64	3	curve	curve	NN
cord-277909-rn1dow26	64	4	analysis	analysis	NN
cord-277909-rn1dow26	64	5	,	,	,
cord-277909-rn1dow26	64	6	identical	identical	JJ
cord-277909-rn1dow26	64	7	melting	melt	VBG
cord-277909-rn1dow26	64	8	peaks	peak	NNS
cord-277909-rn1dow26	64	9	were	be	VBD
cord-277909-rn1dow26	64	10	observed	observe	VBN
cord-277909-rn1dow26	64	11	in	in	IN
cord-277909-rn1dow26	64	12	all	all	DT
cord-277909-rn1dow26	64	13	parainfluenza	parainfluenza	JJ
cord-277909-rn1dow26	64	14	3	3	CD
cord-277909-rn1dow26	64	15	samples	sample	NNS
cord-277909-rn1dow26	64	16	and	and	CC
cord-277909-rn1dow26	64	17	controls	control	NNS
cord-277909-rn1dow26	64	18	(	(	-LRB-
cord-277909-rn1dow26	64	19	http://probes.invitrogen.com/handbook/figures/0710.html	http://probes.invitrogen.com/handbook/figures/0710.html	NN
cord-277909-rn1dow26	64	20	)	)	-RRB-
cord-277909-rn1dow26	64	21	.	.	.
cord-277909-rn1dow26	65	1	Fig	Fig	NNP
cord-277909-rn1dow26	65	2	.	.	.
cord-277909-rn1dow26	66	1	3	3	LS
cord-277909-rn1dow26	66	2	.	.	.
cord-277909-rn1dow26	67	1	Dual	dual	JJ
cord-277909-rn1dow26	67	2	labelled	label	VBN
cord-277909-rn1dow26	67	3	probes	probe	NNS
cord-277909-rn1dow26	67	4	(	(	-LRB-
cord-277909-rn1dow26	67	5	also	also	RB
cord-277909-rn1dow26	67	6	known	know	VBN
cord-277909-rn1dow26	67	7	as	as	IN
cord-277909-rn1dow26	67	8	Taqman	Taqman	NNP
cord-277909-rn1dow26	67	9	TM	TM	NNP
cord-277909-rn1dow26	67	10	probes	probe	NNS
cord-277909-rn1dow26	67	11	)	)	-RRB-
cord-277909-rn1dow26	67	12	are	be	VBP
cord-277909-rn1dow26	67	13	oligonucleotides	oligonucleotide	NNS
cord-277909-rn1dow26	67	14	that	that	WDT
cord-277909-rn1dow26	67	15	contain	contain	VBP
cord-277909-rn1dow26	67	16	a	a	DT
cord-277909-rn1dow26	67	17	fluorescent	fluorescent	JJ
cord-277909-rn1dow26	67	18	dye	dye	NN
cord-277909-rn1dow26	67	19	on	on	IN
cord-277909-rn1dow26	67	20	the	the	DT
cord-277909-rn1dow26	67	21	5	5	CD
cord-277909-rn1dow26	67	22	base	base	NN
cord-277909-rn1dow26	67	23	,	,	,
cord-277909-rn1dow26	67	24	and	and	CC
cord-277909-rn1dow26	67	25	a	a	DT
cord-277909-rn1dow26	67	26	quenching	quenching	NN
cord-277909-rn1dow26	67	27	dye	dye	NN
cord-277909-rn1dow26	67	28	located	locate	VBN
cord-277909-rn1dow26	67	29	on	on	IN
cord-277909-rn1dow26	67	30	the	the	DT
cord-277909-rn1dow26	67	31	3	3	CD
cord-277909-rn1dow26	67	32	base	base	NN
cord-277909-rn1dow26	67	33	.	.	.
cord-277909-rn1dow26	68	1	When	when	WRB
cord-277909-rn1dow26	68	2	excited	excite	VBN
cord-277909-rn1dow26	68	3	the	the	DT
cord-277909-rn1dow26	68	4	flourescent	flourescent	NN
cord-277909-rn1dow26	68	5	dye	dye	NN
cord-277909-rn1dow26	68	6	transfers	transfer	NNS
cord-277909-rn1dow26	68	7	energy	energy	NN
cord-277909-rn1dow26	68	8	to	to	IN
cord-277909-rn1dow26	68	9	the	the	DT
cord-277909-rn1dow26	68	10	nearby	nearby	JJ
cord-277909-rn1dow26	68	11	quenching	quenching	NN
cord-277909-rn1dow26	68	12	dye	dye	NN
cord-277909-rn1dow26	68	13	molecule	molecule	NN
cord-277909-rn1dow26	68	14	rather	rather	RB
cord-277909-rn1dow26	68	15	than	than	IN
cord-277909-rn1dow26	68	16	fluorescing	fluoresce	VBG
cord-277909-rn1dow26	68	17	,	,	,
cord-277909-rn1dow26	68	18	resulting	result	VBG
cord-277909-rn1dow26	68	19	in	in	IN
cord-277909-rn1dow26	68	20	a	a	DT
cord-277909-rn1dow26	68	21	non	non	JJ
cord-277909-rn1dow26	68	22	-	-	JJ
cord-277909-rn1dow26	68	23	fluorescent	fluorescent	JJ
cord-277909-rn1dow26	68	24	probe	probe	NN
cord-277909-rn1dow26	68	25	.	.	.
cord-277909-rn1dow26	69	1	Dual	dual	JJ
cord-277909-rn1dow26	69	2	labelled	label	VBN
cord-277909-rn1dow26	69	3	probes	probe	NNS
cord-277909-rn1dow26	69	4	are	be	VBP
cord-277909-rn1dow26	69	5	designed	design	VBN
cord-277909-rn1dow26	69	6	to	to	TO
cord-277909-rn1dow26	69	7	hybridize	hybridize	VB
cord-277909-rn1dow26	69	8	to	to	IN
cord-277909-rn1dow26	69	9	an	an	DT
cord-277909-rn1dow26	69	10	internal	internal	JJ
cord-277909-rn1dow26	69	11	region	region	NN
cord-277909-rn1dow26	69	12	of	of	IN
cord-277909-rn1dow26	69	13	a	a	DT
cord-277909-rn1dow26	69	14	PCR	PCR	NNP
cord-277909-rn1dow26	69	15	product	product	NN
cord-277909-rn1dow26	69	16	.	.	.
cord-277909-rn1dow26	70	1	During	during	IN
cord-277909-rn1dow26	70	2	PCR	PCR	NNP
cord-277909-rn1dow26	70	3	,	,	,
cord-277909-rn1dow26	70	4	when	when	WRB
cord-277909-rn1dow26	70	5	the	the	DT
cord-277909-rn1dow26	70	6	polymerase	polymerase	NN
cord-277909-rn1dow26	70	7	replicates	replicate	VBZ
cord-277909-rn1dow26	70	8	a	a	DT
cord-277909-rn1dow26	70	9	template	template	NN
cord-277909-rn1dow26	70	10	on	on	IN
cord-277909-rn1dow26	70	11	which	which	WDT
cord-277909-rn1dow26	70	12	the	the	DT
cord-277909-rn1dow26	70	13	probe	probe	NN
cord-277909-rn1dow26	70	14	is	be	VBZ
cord-277909-rn1dow26	70	15	bound	bind	VBN
cord-277909-rn1dow26	70	16	,	,	,
cord-277909-rn1dow26	70	17	the	the	DT
cord-277909-rn1dow26	70	18	5	5	CD
cord-277909-rn1dow26	70	19	-exonuclease	-exonuclease	NN
cord-277909-rn1dow26	70	20	activity	activity	NN
cord-277909-rn1dow26	70	21	of	of	IN
cord-277909-rn1dow26	70	22	the	the	DT
cord-277909-rn1dow26	70	23	polymerase	polymerase	NN
cord-277909-rn1dow26	70	24	cleaves	cleave	VBZ
cord-277909-rn1dow26	70	25	the	the	DT
cord-277909-rn1dow26	70	26	probe	probe	NN
cord-277909-rn1dow26	70	27	.	.	.
cord-277909-rn1dow26	71	1	This	this	DT
cord-277909-rn1dow26	71	2	separates	separate	VBZ
cord-277909-rn1dow26	71	3	the	the	DT
cord-277909-rn1dow26	71	4	fluorescent	fluorescent	NN
cord-277909-rn1dow26	71	5	and	and	CC
cord-277909-rn1dow26	71	6	quenching	quench	VBG
cord-277909-rn1dow26	71	7	dyes	dye	NNS
cord-277909-rn1dow26	71	8	and	and	CC
cord-277909-rn1dow26	71	9	FRET	fret	VB
cord-277909-rn1dow26	71	10	no	no	RB
cord-277909-rn1dow26	71	11	longer	long	RBR
cord-277909-rn1dow26	71	12	occurs	occur	VBZ
cord-277909-rn1dow26	71	13	,	,	,
cord-277909-rn1dow26	71	14	allowing	allow	VBG
cord-277909-rn1dow26	71	15	detection	detection	NN
cord-277909-rn1dow26	71	16	of	of	IN
cord-277909-rn1dow26	71	17	the	the	DT
cord-277909-rn1dow26	71	18	signal	signal	NN
cord-277909-rn1dow26	71	19	from	from	IN
cord-277909-rn1dow26	71	20	the	the	DT
cord-277909-rn1dow26	71	21	reporter	reporter	NN
cord-277909-rn1dow26	71	22	dye	dye	NN
cord-277909-rn1dow26	71	23	.	.	.
cord-277909-rn1dow26	72	1	Fluorescence	fluorescence	NN
cord-277909-rn1dow26	72	2	increases	increase	VBZ
cord-277909-rn1dow26	72	3	in	in	IN
cord-277909-rn1dow26	72	4	each	each	DT
cord-277909-rn1dow26	72	5	cycle	cycle	NN
cord-277909-rn1dow26	72	6	,	,	,
cord-277909-rn1dow26	72	7	proportional	proportional	JJ
cord-277909-rn1dow26	72	8	to	to	IN
cord-277909-rn1dow26	72	9	the	the	DT
cord-277909-rn1dow26	72	10	rate	rate	NN
cord-277909-rn1dow26	72	11	of	of	IN
cord-277909-rn1dow26	72	12	probe	probe	NN
cord-277909-rn1dow26	72	13	cleavage	cleavage	NN
cord-277909-rn1dow26	72	14	.	.	.
cord-277909-rn1dow26	73	1	change	change	NN
cord-277909-rn1dow26	73	2	in	in	IN
cord-277909-rn1dow26	73	3	fluorescence	fluorescence	NN
cord-277909-rn1dow26	73	4	(	(	-LRB-
cord-277909-rn1dow26	73	5	R	r	NN
cord-277909-rn1dow26	73	6	n	n	NN
cord-277909-rn1dow26	73	7	)	)	-RRB-
cord-277909-rn1dow26	73	8	produced	produce	VBN
cord-277909-rn1dow26	73	9	during	during	IN
cord-277909-rn1dow26	73	10	successful	successful	JJ
cord-277909-rn1dow26	73	11	real	real	JJ
cord-277909-rn1dow26	73	12	time	time	NN
cord-277909-rn1dow26	73	13	PCR	PCR	NNP
cord-277909-rn1dow26	73	14	.	.	.
cord-277909-rn1dow26	74	1	We	-PRON-	PRP
cord-277909-rn1dow26	74	2	have	have	VBP
cord-277909-rn1dow26	74	3	found	find	VBN
cord-277909-rn1dow26	74	4	in	in	IN
cord-277909-rn1dow26	74	5	most	most	JJS
cord-277909-rn1dow26	74	6	,	,	,
cord-277909-rn1dow26	74	7	but	but	CC
cord-277909-rn1dow26	74	8	not	not	RB
cord-277909-rn1dow26	74	9	all	all	DT
cord-277909-rn1dow26	74	10	cases	case	NNS
cord-277909-rn1dow26	74	11	,	,	,
cord-277909-rn1dow26	74	12	that	that	IN
cord-277909-rn1dow26	74	13	dual	dual	JJ
cord-277909-rn1dow26	74	14	labelled	label	VBN
cord-277909-rn1dow26	74	15	probes	probe	NNS
cord-277909-rn1dow26	74	16	produce	produce	VBP
cord-277909-rn1dow26	74	17	a	a	DT
cord-277909-rn1dow26	74	18	greater	great	JJR
cord-277909-rn1dow26	74	19	fluorescent	fluorescent	NN
cord-277909-rn1dow26	74	20	change	change	NN
cord-277909-rn1dow26	74	21	than	than	IN
cord-277909-rn1dow26	74	22	molecular	molecular	JJ
cord-277909-rn1dow26	74	23	beacons	beacon	NNS
cord-277909-rn1dow26	74	24	(	(	-LRB-
cord-277909-rn1dow26	74	25	Fig	Fig	NNP
cord-277909-rn1dow26	74	26	.	.	NNP
cord-277909-rn1dow26	74	27	4	4	CD
cord-277909-rn1dow26	74	28	)	)	-RRB-
cord-277909-rn1dow26	74	29	.	.	.
cord-277909-rn1dow26	75	1	A	a	DT
cord-277909-rn1dow26	75	2	larger	large	JJR
cord-277909-rn1dow26	75	3	R	r	NN
cord-277909-rn1dow26	75	4	n	n	NN
cord-277909-rn1dow26	75	5	allows	allow	VBZ
cord-277909-rn1dow26	75	6	easier	easy	JJR
cord-277909-rn1dow26	75	7	interpretation	interpretation	NN
cord-277909-rn1dow26	75	8	of	of	IN
cord-277909-rn1dow26	75	9	results	result	NNS
cord-277909-rn1dow26	75	10	,	,	,
cord-277909-rn1dow26	75	11	as	as	IN
cord-277909-rn1dow26	75	12	low	low	JJ
cord-277909-rn1dow26	75	13	positive	positive	JJ
cord-277909-rn1dow26	75	14	results	result	NNS
cord-277909-rn1dow26	75	15	maybe	maybe	RB
cord-277909-rn1dow26	75	16	more	more	RBR
cord-277909-rn1dow26	75	17	easily	easily	RB
cord-277909-rn1dow26	75	18	differentiated	differentiate	VBN
cord-277909-rn1dow26	75	19	from	from	IN
cord-277909-rn1dow26	75	20	the	the	DT
cord-277909-rn1dow26	75	21	variable	variable	JJ
cord-277909-rn1dow26	75	22	background	background	NN
cord-277909-rn1dow26	75	23	.	.	.
cord-277909-rn1dow26	76	1	Dual	dual	JJ
cord-277909-rn1dow26	76	2	labelled	label	VBN
cord-277909-rn1dow26	76	3	probes	probe	NNS
cord-277909-rn1dow26	76	4	provide	provide	VBP
cord-277909-rn1dow26	76	5	a	a	DT
cord-277909-rn1dow26	76	6	greater	great	JJR
cord-277909-rn1dow26	76	7	fluorescent	fluorescent	NN
cord-277909-rn1dow26	76	8	change	change	NN
cord-277909-rn1dow26	76	9	as	as	IN
cord-277909-rn1dow26	76	10	the	the	DT
cord-277909-rn1dow26	76	11	reporter	reporter	NN
cord-277909-rn1dow26	76	12	dye	dye	NN
cord-277909-rn1dow26	76	13	is	be	VBZ
cord-277909-rn1dow26	76	14	irreversibly	irreversibly	RB
cord-277909-rn1dow26	76	15	released	release	VBN
cord-277909-rn1dow26	76	16	from	from	IN
cord-277909-rn1dow26	76	17	the	the	DT
cord-277909-rn1dow26	76	18	quencher	quencher	NN
cord-277909-rn1dow26	76	19	during	during	IN
cord-277909-rn1dow26	76	20	the	the	DT
cord-277909-rn1dow26	76	21	extension	extension	NN
cord-277909-rn1dow26	76	22	stage	stage	NN
cord-277909-rn1dow26	76	23	of	of	IN
cord-277909-rn1dow26	76	24	each	each	DT
cord-277909-rn1dow26	76	25	PCR	PCR	NNP
cord-277909-rn1dow26	76	26	cycle	cycle	NN
cord-277909-rn1dow26	76	27	.	.	.
cord-277909-rn1dow26	77	1	Consequently	consequently	RB
cord-277909-rn1dow26	77	2	there	there	EX
cord-277909-rn1dow26	77	3	is	be	VBZ
cord-277909-rn1dow26	77	4	a	a	DT
cord-277909-rn1dow26	77	5	cumulative	cumulative	JJ
cord-277909-rn1dow26	77	6	and	and	CC
cord-277909-rn1dow26	77	7	permanent	permanent	JJ
cord-277909-rn1dow26	77	8	record	record	NN
cord-277909-rn1dow26	77	9	of	of	IN
cord-277909-rn1dow26	77	10	successful	successful	JJ
cord-277909-rn1dow26	77	11	amplification	amplification	NN
cord-277909-rn1dow26	77	12	,	,	,
cord-277909-rn1dow26	77	13	which	which	WDT
cord-277909-rn1dow26	77	14	is	be	VBZ
cord-277909-rn1dow26	77	15	added	add	VBN
cord-277909-rn1dow26	77	16	to	to	IN
cord-277909-rn1dow26	77	17	during	during	IN
cord-277909-rn1dow26	77	18	subsequent	subsequent	JJ
cord-277909-rn1dow26	77	19	PCR	PCR	NNP
cord-277909-rn1dow26	77	20	cycles	cycle	NNS
cord-277909-rn1dow26	77	21	.	.	.
cord-277909-rn1dow26	78	1	Molecular	molecular	JJ
cord-277909-rn1dow26	78	2	beacons	beacon	NNS
cord-277909-rn1dow26	78	3	are	be	VBP
cord-277909-rn1dow26	78	4	not	not	RB
cord-277909-rn1dow26	78	5	destroyed	destroy	VBN
cord-277909-rn1dow26	78	6	at	at	IN
cord-277909-rn1dow26	78	7	the	the	DT
cord-277909-rn1dow26	78	8	end	end	NN
cord-277909-rn1dow26	78	9	of	of	IN
cord-277909-rn1dow26	78	10	each	each	DT
cord-277909-rn1dow26	78	11	cycle	cycle	NN
cord-277909-rn1dow26	78	12	,	,	,
cord-277909-rn1dow26	78	13	but	but	CC
cord-277909-rn1dow26	78	14	return	return	VB
cord-277909-rn1dow26	78	15	to	to	IN
cord-277909-rn1dow26	78	16	free	free	JJ
cord-277909-rn1dow26	78	17	solution	solution	NN
cord-277909-rn1dow26	78	18	during	during	IN
cord-277909-rn1dow26	78	19	the	the	DT
cord-277909-rn1dow26	78	20	denaturation	denaturation	NN
cord-277909-rn1dow26	78	21	phase	phase	NN
cord-277909-rn1dow26	78	22	and	and	CC
cord-277909-rn1dow26	78	23	revert	revert	VB
cord-277909-rn1dow26	78	24	back	back	RB
cord-277909-rn1dow26	78	25	to	to	IN
cord-277909-rn1dow26	78	26	their	-PRON-	PRP$
cord-277909-rn1dow26	78	27	hairpin	hairpin	JJ
cord-277909-rn1dow26	78	28	-	-	HYPH
cord-277909-rn1dow26	78	29	loop	loop	NN
cord-277909-rn1dow26	78	30	structure	structure	NN
cord-277909-rn1dow26	78	31	.	.	.
cord-277909-rn1dow26	79	1	Consequently	consequently	RB
cord-277909-rn1dow26	79	2	there	there	EX
cord-277909-rn1dow26	79	3	is	be	VBZ
cord-277909-rn1dow26	79	4	no	no	DT
cord-277909-rn1dow26	79	5	accumulation	accumulation	NN
cord-277909-rn1dow26	79	6	of	of	IN
cord-277909-rn1dow26	79	7	free	free	JJ
cord-277909-rn1dow26	79	8	reporter	reporter	NN
cord-277909-rn1dow26	79	9	dye	dye	NN
cord-277909-rn1dow26	79	10	and	and	CC
cord-277909-rn1dow26	79	11	the	the	DT
cord-277909-rn1dow26	79	12	extra	extra	JJ
cord-277909-rn1dow26	79	13	fluorescence	fluorescence	NN
cord-277909-rn1dow26	79	14	produced	produce	VBN
cord-277909-rn1dow26	79	15	is	be	VBZ
cord-277909-rn1dow26	79	16	less	less	JJR
cord-277909-rn1dow26	79	17	after	after	IN
cord-277909-rn1dow26	79	18	each	each	DT
cord-277909-rn1dow26	79	19	cycle	cycle	NN
cord-277909-rn1dow26	79	20	than	than	IN
cord-277909-rn1dow26	79	21	when	when	WRB
cord-277909-rn1dow26	79	22	compared	compare	VBN
cord-277909-rn1dow26	79	23	to	to	IN
cord-277909-rn1dow26	79	24	dual	dual	JJ
cord-277909-rn1dow26	79	25	labelled	label	VBN
cord-277909-rn1dow26	79	26	probes	probe	NNS
cord-277909-rn1dow26	79	27	.	.	.
cord-277909-rn1dow26	80	1	Since	since	IN
cord-277909-rn1dow26	80	2	real	real	JJ
cord-277909-rn1dow26	80	3	time	time	NN
cord-277909-rn1dow26	80	4	PCR	PCR	NNP
cord-277909-rn1dow26	80	5	is	be	VBZ
cord-277909-rn1dow26	80	6	a	a	DT
cord-277909-rn1dow26	80	7	relatively	relatively	RB
cord-277909-rn1dow26	80	8	new	new	JJ
cord-277909-rn1dow26	80	9	technique	technique	NN
cord-277909-rn1dow26	80	10	,	,	,
cord-277909-rn1dow26	80	11	published	publish	VBN
cord-277909-rn1dow26	80	12	assays	assay	NNS
cord-277909-rn1dow26	80	13	may	may	MD
cord-277909-rn1dow26	80	14	not	not	RB
cord-277909-rn1dow26	80	15	be	be	VB
cord-277909-rn1dow26	80	16	available	available	JJ
cord-277909-rn1dow26	80	17	for	for	IN
cord-277909-rn1dow26	80	18	all	all	DT
cord-277909-rn1dow26	80	19	viral	viral	JJ
cord-277909-rn1dow26	80	20	pathogens	pathogen	NNS
cord-277909-rn1dow26	80	21	.	.	.
cord-277909-rn1dow26	81	1	As	as	IN
cord-277909-rn1dow26	81	2	a	a	DT
cord-277909-rn1dow26	81	3	result	result	NN
cord-277909-rn1dow26	81	4	many	many	JJ
cord-277909-rn1dow26	81	5	laboratories	laboratory	NNS
cord-277909-rn1dow26	81	6	may	may	MD
cord-277909-rn1dow26	81	7	wish	wish	VB
cord-277909-rn1dow26	81	8	to	to	TO
cord-277909-rn1dow26	81	9	develop	develop	VB
cord-277909-rn1dow26	81	10	novel	novel	NN
cord-277909-rn1dow26	81	11	in	in	IN
cord-277909-rn1dow26	81	12	-	-	HYPH
cord-277909-rn1dow26	81	13	house	house	NN
cord-277909-rn1dow26	81	14	real	real	JJ
cord-277909-rn1dow26	81	15	time	time	NN
cord-277909-rn1dow26	81	16	PCR	PCR	NNP
cord-277909-rn1dow26	81	17	assays	assay	NNS
cord-277909-rn1dow26	81	18	.	.	.
cord-277909-rn1dow26	82	1	The	the	DT
cord-277909-rn1dow26	82	2	initial	initial	JJ
cord-277909-rn1dow26	82	3	stages	stage	NNS
cord-277909-rn1dow26	82	4	in	in	IN
cord-277909-rn1dow26	82	5	developing	develop	VBG
cord-277909-rn1dow26	82	6	a	a	DT
cord-277909-rn1dow26	82	7	real	real	JJ
cord-277909-rn1dow26	82	8	time	time	NN
cord-277909-rn1dow26	82	9	PCR	pcr	NN
cord-277909-rn1dow26	82	10	assay	assay	NN
cord-277909-rn1dow26	82	11	are	be	VBP
cord-277909-rn1dow26	82	12	the	the	DT
cord-277909-rn1dow26	82	13	same	same	JJ
cord-277909-rn1dow26	82	14	as	as	IN
cord-277909-rn1dow26	82	15	those	those	DT
cord-277909-rn1dow26	82	16	required	require	VBN
cord-277909-rn1dow26	82	17	for	for	IN
cord-277909-rn1dow26	82	18	designing	design	VBG
cord-277909-rn1dow26	82	19	traditional	traditional	JJ
cord-277909-rn1dow26	82	20	gel	gel	NN
cord-277909-rn1dow26	82	21	based	base	VBN
cord-277909-rn1dow26	82	22	PCR	PCR	NNP
cord-277909-rn1dow26	82	23	tests	test	NNS
cord-277909-rn1dow26	82	24	.	.	.
cord-277909-rn1dow26	83	1	The	the	DT
cord-277909-rn1dow26	83	2	first	first	JJ
cord-277909-rn1dow26	83	3	step	step	NN
cord-277909-rn1dow26	83	4	is	be	VBZ
cord-277909-rn1dow26	83	5	to	to	TO
cord-277909-rn1dow26	83	6	identify	identify	VB
cord-277909-rn1dow26	83	7	a	a	DT
cord-277909-rn1dow26	83	8	conserved	conserved	JJ
cord-277909-rn1dow26	83	9	region	region	NN
cord-277909-rn1dow26	83	10	of	of	IN
cord-277909-rn1dow26	83	11	the	the	DT
cord-277909-rn1dow26	83	12	viral	viral	NN
cord-277909-rn1dow26	83	13	(	(	-LRB-
cord-277909-rn1dow26	83	14	or	or	CC
cord-277909-rn1dow26	83	15	other	other	JJ
cord-277909-rn1dow26	83	16	pathogen	pathogen	NN
cord-277909-rn1dow26	83	17	)	)	-RRB-
cord-277909-rn1dow26	83	18	genome	genome	NN
cord-277909-rn1dow26	83	19	in	in	IN
cord-277909-rn1dow26	83	20	which	which	WDT
cord-277909-rn1dow26	83	21	to	to	TO
cord-277909-rn1dow26	83	22	design	design	VB
cord-277909-rn1dow26	83	23	the	the	DT
cord-277909-rn1dow26	83	24	assay	assay	NN
cord-277909-rn1dow26	83	25	.	.	.
cord-277909-rn1dow26	84	1	A	a	DT
cord-277909-rn1dow26	84	2	literature	literature	NN
cord-277909-rn1dow26	84	3	review	review	NN
cord-277909-rn1dow26	84	4	will	will	MD
cord-277909-rn1dow26	84	5	often	often	RB
cord-277909-rn1dow26	84	6	reveal	reveal	VB
cord-277909-rn1dow26	84	7	which	which	WDT
cord-277909-rn1dow26	84	8	genes	gene	NNS
cord-277909-rn1dow26	84	9	are	be	VBP
cord-277909-rn1dow26	84	10	conserved	conserve	VBN
cord-277909-rn1dow26	84	11	,	,	,
cord-277909-rn1dow26	84	12	and	and	CC
cord-277909-rn1dow26	84	13	most	most	RBS
cord-277909-rn1dow26	84	14	often	often	RB
cord-277909-rn1dow26	84	15	these	these	DT
cord-277909-rn1dow26	84	16	will	will	MD
cord-277909-rn1dow26	84	17	be	be	VB
cord-277909-rn1dow26	84	18	genes	gene	NNS
cord-277909-rn1dow26	84	19	encoding	encode	VBG
cord-277909-rn1dow26	84	20	nonimmunogenic	nonimmunogenic	NNP
cord-277909-rn1dow26	84	21	proteins	protein	NNS
cord-277909-rn1dow26	84	22	.	.	.
cord-277909-rn1dow26	85	1	Once	once	RB
cord-277909-rn1dow26	85	2	a	a	DT
cord-277909-rn1dow26	85	3	gene	gene	NN
cord-277909-rn1dow26	85	4	is	be	VBZ
cord-277909-rn1dow26	85	5	identified	identify	VBN
cord-277909-rn1dow26	85	6	,	,	,
cord-277909-rn1dow26	85	7	a	a	DT
cord-277909-rn1dow26	85	8	BLAST	blast	NN
cord-277909-rn1dow26	85	9	search	search	NN
cord-277909-rn1dow26	85	10	(	(	-LRB-
cord-277909-rn1dow26	85	11	http://www.ncbi.nlm.nih.gov/BLAST/	http://www.ncbi.nlm.nih.gov/BLAST/	NNP
cord-277909-rn1dow26	85	12	)	)	-RRB-
cord-277909-rn1dow26	85	13	is	be	VBZ
cord-277909-rn1dow26	85	14	performed	perform	VBN
cord-277909-rn1dow26	85	15	to	to	TO
cord-277909-rn1dow26	85	16	locate	locate	VB
cord-277909-rn1dow26	85	17	the	the	DT
cord-277909-rn1dow26	85	18	most	most	RBS
cord-277909-rn1dow26	85	19	conserved	conserved	JJ
cord-277909-rn1dow26	85	20	regions	region	NNS
cord-277909-rn1dow26	85	21	within	within	IN
cord-277909-rn1dow26	85	22	this	this	DT
cord-277909-rn1dow26	85	23	gene	gene	NN
cord-277909-rn1dow26	85	24	.	.	.
cord-277909-rn1dow26	86	1	As	as	IN
cord-277909-rn1dow26	86	2	real	real	JJ
cord-277909-rn1dow26	86	3	time	time	NN
cord-277909-rn1dow26	86	4	amplicons	amplicon	NNS
cord-277909-rn1dow26	86	5	are	be	VBP
cord-277909-rn1dow26	86	6	short	short	JJ
cord-277909-rn1dow26	86	7	and	and	CC
cord-277909-rn1dow26	86	8	contain	contain	VBP
cord-277909-rn1dow26	86	9	a	a	DT
cord-277909-rn1dow26	86	10	third	third	JJ
cord-277909-rn1dow26	86	11	oligonucleotide	oligonucleotide	NN
cord-277909-rn1dow26	86	12	(	(	-LRB-
cord-277909-rn1dow26	86	13	i.e.	i.e.	FW
cord-277909-rn1dow26	86	14	,	,	,
cord-277909-rn1dow26	86	15	the	the	DT
cord-277909-rn1dow26	86	16	probe	probe	NN
cord-277909-rn1dow26	86	17	)	)	-RRB-
cord-277909-rn1dow26	86	18	,	,	,
cord-277909-rn1dow26	86	19	the	the	DT
cord-277909-rn1dow26	86	20	ideal	ideal	JJ
cord-277909-rn1dow26	86	21	region	region	NN
cord-277909-rn1dow26	86	22	to	to	TO
cord-277909-rn1dow26	86	23	design	design	VB
cord-277909-rn1dow26	86	24	an	an	DT
cord-277909-rn1dow26	86	25	assay	assay	NN
cord-277909-rn1dow26	86	26	would	would	MD
cord-277909-rn1dow26	86	27	be	be	VB
cord-277909-rn1dow26	86	28	100	100	CD
cord-277909-rn1dow26	86	29	-	-	SYM
cord-277909-rn1dow26	86	30	150	150	CD
cord-277909-rn1dow26	86	31	nucleotides	nucleotide	NNS
cord-277909-rn1dow26	86	32	long	long	JJ
cord-277909-rn1dow26	86	33	with	with	IN
cord-277909-rn1dow26	86	34	3	3	CD
cord-277909-rn1dow26	86	35	regions	region	NNS
cord-277909-rn1dow26	86	36	of	of	IN
cord-277909-rn1dow26	86	37	30	30	CD
cord-277909-rn1dow26	86	38	bases	basis	NNS
cord-277909-rn1dow26	86	39	devoid	devoid	JJ
cord-277909-rn1dow26	86	40	of	of	IN
cord-277909-rn1dow26	86	41	all	all	DT
cord-277909-rn1dow26	86	42	base	base	NN
cord-277909-rn1dow26	86	43	degeneracies	degeneracy	NNS
cord-277909-rn1dow26	86	44	.	.	.
cord-277909-rn1dow26	87	1	It	-PRON-	PRP
cord-277909-rn1dow26	87	2	is	be	VBZ
cord-277909-rn1dow26	87	3	best	good	JJS
cord-277909-rn1dow26	87	4	to	to	TO
cord-277909-rn1dow26	87	5	find	find	VB
cord-277909-rn1dow26	87	6	a	a	DT
cord-277909-rn1dow26	87	7	conserved	conserved	JJ
cord-277909-rn1dow26	87	8	region	region	NN
cord-277909-rn1dow26	87	9	of	of	IN
cord-277909-rn1dow26	87	10	400	400	CD
cord-277909-rn1dow26	87	11	-	-	SYM
cord-277909-rn1dow26	87	12	500	500	CD
cord-277909-rn1dow26	87	13	bases	basis	NNS
cord-277909-rn1dow26	87	14	to	to	TO
cord-277909-rn1dow26	87	15	allow	allow	VB
cord-277909-rn1dow26	87	16	the	the	DT
cord-277909-rn1dow26	87	17	software	software	NN
cord-277909-rn1dow26	87	18	to	to	TO
cord-277909-rn1dow26	87	19	identify	identify	VB
cord-277909-rn1dow26	87	20	a	a	DT
cord-277909-rn1dow26	87	21	number	number	NN
cord-277909-rn1dow26	87	22	of	of	IN
cord-277909-rn1dow26	87	23	potential	potential	JJ
cord-277909-rn1dow26	87	24	assays	assay	NNS
cord-277909-rn1dow26	87	25	.	.	.
cord-277909-rn1dow26	88	1	Several	several	JJ
cord-277909-rn1dow26	88	2	software	software	NN
cord-277909-rn1dow26	88	3	programs	program	NNS
cord-277909-rn1dow26	88	4	are	be	VBP
cord-277909-rn1dow26	88	5	available	available	JJ
cord-277909-rn1dow26	88	6	to	to	TO
cord-277909-rn1dow26	88	7	design	design	VB
cord-277909-rn1dow26	88	8	real	real	JJ
cord-277909-rn1dow26	88	9	time	time	NN
cord-277909-rn1dow26	88	10	assays	assay	NNS
cord-277909-rn1dow26	88	11	,	,	,
cord-277909-rn1dow26	88	12	and	and	CC
cord-277909-rn1dow26	88	13	often	often	RB
cord-277909-rn1dow26	88	14	software	software	NN
cord-277909-rn1dow26	88	15	is	be	VBZ
cord-277909-rn1dow26	88	16	provided	provide	VBN
cord-277909-rn1dow26	88	17	by	by	IN
cord-277909-rn1dow26	88	18	the	the	DT
cord-277909-rn1dow26	88	19	instrument	instrument	NN
cord-277909-rn1dow26	88	20	supplier	supplier	NN
cord-277909-rn1dow26	88	21	.	.	.
cord-277909-rn1dow26	89	1	Beacon	Beacon	NNP
cord-277909-rn1dow26	89	2	Designer	Designer	NNP
cord-277909-rn1dow26	89	3	(	(	-LRB-
cord-277909-rn1dow26	90	1	Pre-	Pre-	NNP
cord-277909-rn1dow26	90	2	Fig	Fig	NNP
cord-277909-rn1dow26	90	3	.	.	.
cord-277909-rn1dow26	91	1	4	4	LS
cord-277909-rn1dow26	91	2	.	.	.
cord-277909-rn1dow26	92	1	Comparison	comparison	NN
cord-277909-rn1dow26	92	2	of	of	IN
cord-277909-rn1dow26	92	3	the	the	DT
cord-277909-rn1dow26	92	4	R	r	NN
cord-277909-rn1dow26	92	5	n	n	NN
cord-277909-rn1dow26	92	6	produced	produce	VBD
cord-277909-rn1dow26	92	7	when	when	WRB
cord-277909-rn1dow26	92	8	using	use	VBG
cord-277909-rn1dow26	92	9	dual	dual	JJ
cord-277909-rn1dow26	92	10	labelled	label	VBN
cord-277909-rn1dow26	92	11	probes	probe	NNS
cord-277909-rn1dow26	92	12	(	(	-LRB-
cord-277909-rn1dow26	92	13	A	a	NN
cord-277909-rn1dow26	92	14	)	)	-RRB-
cord-277909-rn1dow26	92	15	vs.	vs.	FW
cord-277909-rn1dow26	92	16	molecular	molecular	JJ
cord-277909-rn1dow26	92	17	beacons	beacon	NNS
cord-277909-rn1dow26	92	18	(	(	-LRB-
cord-277909-rn1dow26	92	19	B	b	NN
cord-277909-rn1dow26	92	20	)	)	-RRB-
cord-277909-rn1dow26	92	21	.	.	.
cord-277909-rn1dow26	93	1	Table	table	NN
cord-277909-rn1dow26	93	2	2	2	CD
cord-277909-rn1dow26	94	1	Main	main	JJ
cord-277909-rn1dow26	94	2	factors	factor	NNS
cord-277909-rn1dow26	94	3	to	to	TO
cord-277909-rn1dow26	94	4	consider	consider	VB
cord-277909-rn1dow26	94	5	when	when	WRB
cord-277909-rn1dow26	94	6	developing	develop	VBG
cord-277909-rn1dow26	94	7	a	a	DT
cord-277909-rn1dow26	94	8	dual	dual	JJ
cord-277909-rn1dow26	94	9	labelled	label	VBN
cord-277909-rn1dow26	94	10	probe	probe	NN
cord-277909-rn1dow26	94	11	PCR	pcr	NN
cord-277909-rn1dow26	94	12	assay	assay	NN
cord-277909-rn1dow26	94	13	Factors	factor	NNS
cord-277909-rn1dow26	94	14	to	to	TO
cord-277909-rn1dow26	94	15	consider	consider	VB
cord-277909-rn1dow26	94	16	when	when	WRB
cord-277909-rn1dow26	94	17	developing	develop	VBG
cord-277909-rn1dow26	94	18	a	a	DT
cord-277909-rn1dow26	94	19	dual	dual	JJ
cord-277909-rn1dow26	94	20	labelled	label	VBN
cord-277909-rn1dow26	94	21	probe	probe	NN
cord-277909-rn1dow26	94	22	PCR	pcr	NN
cord-277909-rn1dow26	94	23	assay	assay	NN
cord-277909-rn1dow26	94	24	Identify	identify	VB
cord-277909-rn1dow26	94	25	a	a	DT
cord-277909-rn1dow26	94	26	conserved	conserved	JJ
cord-277909-rn1dow26	94	27	region	region	NN
cord-277909-rn1dow26	94	28	of	of	IN
cord-277909-rn1dow26	94	29	the	the	DT
cord-277909-rn1dow26	94	30	viral	viral	NN
cord-277909-rn1dow26	94	31	(	(	-LRB-
cord-277909-rn1dow26	94	32	or	or	CC
cord-277909-rn1dow26	94	33	other	other	JJ
cord-277909-rn1dow26	94	34	pathogen	pathogen	NN
cord-277909-rn1dow26	94	35	)	)	-RRB-
cord-277909-rn1dow26	94	36	genome	genome	NN
cord-277909-rn1dow26	94	37	Identify	identify	VB
cord-277909-rn1dow26	94	38	a	a	DT
cord-277909-rn1dow26	94	39	region	region	NN
cord-277909-rn1dow26	94	40	within	within	IN
cord-277909-rn1dow26	94	41	this	this	DT
cord-277909-rn1dow26	94	42	area	area	NN
cord-277909-rn1dow26	94	43	of	of	IN
cord-277909-rn1dow26	94	44	∼400	∼400	NNP
cord-277909-rn1dow26	94	45	-	-	HYPH
cord-277909-rn1dow26	94	46	500	500	CD
cord-277909-rn1dow26	94	47	bases	basis	NNS
cord-277909-rn1dow26	94	48	in	in	IN
cord-277909-rn1dow26	94	49	length	length	NN
cord-277909-rn1dow26	94	50	Check	check	NN
cord-277909-rn1dow26	94	51	that	that	IN
cord-277909-rn1dow26	94	52	the	the	DT
cord-277909-rn1dow26	94	53	probe	probe	NN
cord-277909-rn1dow26	94	54	sequence	sequence	NN
cord-277909-rn1dow26	94	55	contains	contain	VBZ
cord-277909-rn1dow26	94	56	more	more	JJR
cord-277909-rn1dow26	94	57	C	c	NN
cord-277909-rn1dow26	94	58	residues	residue	NNS
cord-277909-rn1dow26	94	59	than	than	IN
cord-277909-rn1dow26	94	60	G	g	NN
cord-277909-rn1dow26	94	61	residues	residue	NNS
cord-277909-rn1dow26	94	62	Ensure	ensure	VBP
cord-277909-rn1dow26	94	63	that	that	IN
cord-277909-rn1dow26	94	64	the	the	DT
cord-277909-rn1dow26	94	65	probe	probe	NN
cord-277909-rn1dow26	94	66	does	do	VBZ
cord-277909-rn1dow26	94	67	not	not	RB
cord-277909-rn1dow26	94	68	begin	begin	VB
cord-277909-rn1dow26	94	69	with	with	IN
cord-277909-rn1dow26	94	70	a	a	DT
cord-277909-rn1dow26	94	71	G	g	NN
cord-277909-rn1dow26	95	1	The	the	DT
cord-277909-rn1dow26	95	2	optimal	optimal	NN
cord-277909-rn1dow26	96	1	primer	primer	NNP
cord-277909-rn1dow26	96	2	T	T	NNP
cord-277909-rn1dow26	96	3	m	m	NN
cord-277909-rn1dow26	96	4	values	value	NNS
cord-277909-rn1dow26	96	5	are	be	VBP
cord-277909-rn1dow26	96	6	58	58	CD
cord-277909-rn1dow26	97	1	-60	-60	NNS
cord-277909-rn1dow26	97	2	•	•	NN
cord-277909-rn1dow26	97	3	C	c	NN
cord-277909-rn1dow26	98	1	The	the	DT
cord-277909-rn1dow26	98	2	optimal	optimal	JJ
cord-277909-rn1dow26	98	3	probe	probe	NN
cord-277909-rn1dow26	98	4	T	T	NNP
cord-277909-rn1dow26	98	5	m	m	NN
cord-277909-rn1dow26	98	6	should	should	MD
cord-277909-rn1dow26	98	7	be	be	VB
cord-277909-rn1dow26	99	1	∼10	∼10	NNP
cord-277909-rn1dow26	99	2	•	•	NNP
cord-277909-rn1dow26	99	3	C	C	NNP
cord-277909-rn1dow26	99	4	higher	higher	RBR
cord-277909-rn1dow26	100	1	The	the	DT
cord-277909-rn1dow26	100	2	amplicon	amplicon	NN
cord-277909-rn1dow26	100	3	should	should	MD
cord-277909-rn1dow26	100	4	not	not	RB
cord-277909-rn1dow26	100	5	exceed	exceed	VB
cord-277909-rn1dow26	100	6	150	150	CD
cord-277909-rn1dow26	100	7	bp	bp	NNS
cord-277909-rn1dow26	100	8	in	in	IN
cord-277909-rn1dow26	100	9	length	length	NN
cord-277909-rn1dow26	101	1	Primers	primer	NNS
cord-277909-rn1dow26	101	2	should	should	MD
cord-277909-rn1dow26	101	3	not	not	RB
cord-277909-rn1dow26	101	4	contain	contain	VB
cord-277909-rn1dow26	101	5	more	more	JJR
cord-277909-rn1dow26	101	6	than	than	IN
cord-277909-rn1dow26	101	7	2/5	2/5	CD
cord-277909-rn1dow26	101	8	G	g	NN
cord-277909-rn1dow26	101	9	or	or	CC
cord-277909-rn1dow26	101	10	C	c	NN
cord-277909-rn1dow26	101	11	residues	residue	NNS
cord-277909-rn1dow26	101	12	at	at	IN
cord-277909-rn1dow26	101	13	the	the	DT
cord-277909-rn1dow26	101	14	3	3	CD
cord-277909-rn1dow26	101	15	Check	check	VB
cord-277909-rn1dow26	101	16	the	the	DT
cord-277909-rn1dow26	101	17	amplicon	amplicon	NN
cord-277909-rn1dow26	101	18	for	for	IN
cord-277909-rn1dow26	101	19	secondary	secondary	JJ
cord-277909-rn1dow26	101	20	structure	structure	NN
cord-277909-rn1dow26	101	21	,	,	,
cord-277909-rn1dow26	101	22	and	and	CC
cord-277909-rn1dow26	101	23	for	for	IN
cord-277909-rn1dow26	101	24	specificity	specificity	NN
cord-277909-rn1dow26	101	25	mier	mier	NNP
cord-277909-rn1dow26	101	26	Biosoft	Biosoft	NNP
cord-277909-rn1dow26	101	27	)	)	-RRB-
cord-277909-rn1dow26	101	28	can	can	MD
cord-277909-rn1dow26	101	29	be	be	VB
cord-277909-rn1dow26	101	30	used	use	VBN
cord-277909-rn1dow26	101	31	to	to	TO
cord-277909-rn1dow26	101	32	design	design	VB
cord-277909-rn1dow26	101	33	either	either	DT
cord-277909-rn1dow26	101	34	molecular	molecular	JJ
cord-277909-rn1dow26	101	35	beacon	beacon	NN
cord-277909-rn1dow26	101	36	assays	assay	NNS
cord-277909-rn1dow26	101	37	or	or	CC
cord-277909-rn1dow26	101	38	dual	dual	JJ
cord-277909-rn1dow26	101	39	labelled	label	VBN
cord-277909-rn1dow26	101	40	probe	probe	NN
cord-277909-rn1dow26	101	41	based	base	VBN
cord-277909-rn1dow26	101	42	assays	assay	NNS
cord-277909-rn1dow26	101	43	,	,	,
cord-277909-rn1dow26	101	44	and	and	CC
cord-277909-rn1dow26	101	45	has	have	VBZ
cord-277909-rn1dow26	101	46	additional	additional	JJ
cord-277909-rn1dow26	101	47	functionality	functionality	NN
cord-277909-rn1dow26	101	48	such	such	JJ
cord-277909-rn1dow26	101	49	as	as	IN
cord-277909-rn1dow26	101	50	BLAST	blast	NN
cord-277909-rn1dow26	101	51	and	and	CC
cord-277909-rn1dow26	101	52	secondary	secondary	JJ
cord-277909-rn1dow26	101	53	structure	structure	NN
cord-277909-rn1dow26	101	54	searching	search	VBG
cord-277909-rn1dow26	101	55	.	.	.
cord-277909-rn1dow26	102	1	Primer	Primer	NNP
cord-277909-rn1dow26	102	2	Express	Express	NNP
cord-277909-rn1dow26	102	3	(	(	-LRB-
cord-277909-rn1dow26	102	4	Applied	Applied	NNP
cord-277909-rn1dow26	102	5	Biosystems	Biosystems	NNP
cord-277909-rn1dow26	102	6	)	)	-RRB-
cord-277909-rn1dow26	102	7	is	be	VBZ
cord-277909-rn1dow26	102	8	another	another	DT
cord-277909-rn1dow26	102	9	useful	useful	JJ
cord-277909-rn1dow26	102	10	tool	tool	NN
cord-277909-rn1dow26	102	11	for	for	IN
cord-277909-rn1dow26	102	12	designing	design	VBG
cord-277909-rn1dow26	102	13	Taqman	Taqman	NNP
cord-277909-rn1dow26	102	14	based	base	VBN
cord-277909-rn1dow26	102	15	assays	assay	NNS
cord-277909-rn1dow26	102	16	,	,	,
cord-277909-rn1dow26	102	17	and	and	CC
cord-277909-rn1dow26	102	18	is	be	VBZ
cord-277909-rn1dow26	102	19	the	the	DT
cord-277909-rn1dow26	102	20	only	only	JJ
cord-277909-rn1dow26	102	21	software	software	NN
cord-277909-rn1dow26	102	22	currently	currently	RB
cord-277909-rn1dow26	102	23	available	available	JJ
cord-277909-rn1dow26	102	24	for	for	IN
cord-277909-rn1dow26	102	25	designing	design	VBG
cord-277909-rn1dow26	102	26	assays	assay	NNS
cord-277909-rn1dow26	102	27	based	base	VBN
cord-277909-rn1dow26	102	28	on	on	IN
cord-277909-rn1dow26	102	29	Applied	Applied	NNP
cord-277909-rn1dow26	102	30	Biosystems	Biosystems	NNP
cord-277909-rn1dow26	102	31	(	(	-LRB-
cord-277909-rn1dow26	102	32	ABI	ABI	NNP
cord-277909-rn1dow26	102	33	)	)	-RRB-
cord-277909-rn1dow26	102	34	MGB	MGB	NNP
cord-277909-rn1dow26	102	35	probes	probe	NNS
cord-277909-rn1dow26	102	36	.	.	.
cord-277909-rn1dow26	103	1	Once	once	IN
cord-277909-rn1dow26	103	2	the	the	DT
cord-277909-rn1dow26	103	3	software	software	NN
cord-277909-rn1dow26	103	4	has	have	VBZ
cord-277909-rn1dow26	103	5	suggested	suggest	VBN
cord-277909-rn1dow26	103	6	a	a	DT
cord-277909-rn1dow26	103	7	primer	primer	NN
cord-277909-rn1dow26	103	8	and	and	CC
cord-277909-rn1dow26	103	9	probe	probe	NN
cord-277909-rn1dow26	103	10	set	set	VBD
cord-277909-rn1dow26	103	11	,	,	,
cord-277909-rn1dow26	103	12	it	-PRON-	PRP
cord-277909-rn1dow26	103	13	is	be	VBZ
cord-277909-rn1dow26	103	14	important	important	JJ
cord-277909-rn1dow26	103	15	to	to	TO
cord-277909-rn1dow26	103	16	ensure	ensure	VB
cord-277909-rn1dow26	103	17	that	that	IN
cord-277909-rn1dow26	103	18	they	-PRON-	PRP
cord-277909-rn1dow26	103	19	meet	meet	VBP
cord-277909-rn1dow26	103	20	the	the	DT
cord-277909-rn1dow26	103	21	criteria	criterion	NNS
cord-277909-rn1dow26	103	22	(	(	-LRB-
cord-277909-rn1dow26	103	23	Table	table	NN
cord-277909-rn1dow26	103	24	2	2	CD
cord-277909-rn1dow26	103	25	)	)	-RRB-
cord-277909-rn1dow26	103	26	.	.	.
cord-277909-rn1dow26	104	1	Assuming	assume	VBG
cord-277909-rn1dow26	104	2	the	the	DT
cord-277909-rn1dow26	104	3	primers	primer	NNS
cord-277909-rn1dow26	104	4	and	and	CC
cord-277909-rn1dow26	104	5	probe	probe	NN
cord-277909-rn1dow26	104	6	meet	meet	VB
cord-277909-rn1dow26	104	7	these	these	DT
cord-277909-rn1dow26	104	8	criteria	criterion	NNS
cord-277909-rn1dow26	104	9	,	,	,
cord-277909-rn1dow26	104	10	it	-PRON-	PRP
cord-277909-rn1dow26	104	11	is	be	VBZ
cord-277909-rn1dow26	104	12	advisable	advisable	JJ
cord-277909-rn1dow26	104	13	to	to	TO
cord-277909-rn1dow26	104	14	check	check	VB
cord-277909-rn1dow26	104	15	the	the	DT
cord-277909-rn1dow26	104	16	amplicon	amplicon	NN
cord-277909-rn1dow26	104	17	for	for	IN
cord-277909-rn1dow26	104	18	secondary	secondary	JJ
cord-277909-rn1dow26	104	19	structure	structure	NN
cord-277909-rn1dow26	104	20	,	,	,
cord-277909-rn1dow26	104	21	and	and	CC
cord-277909-rn1dow26	104	22	for	for	IN
cord-277909-rn1dow26	104	23	specificity	specificity	NN
cord-277909-rn1dow26	104	24	.	.	.
cord-277909-rn1dow26	105	1	Secondary	secondary	JJ
cord-277909-rn1dow26	105	2	structure	structure	NN
cord-277909-rn1dow26	105	3	prediction	prediction	NN
cord-277909-rn1dow26	105	4	software	software	NN
cord-277909-rn1dow26	105	5	is	be	VBZ
cord-277909-rn1dow26	105	6	available	available	JJ
cord-277909-rn1dow26	105	7	on	on	IN
cord-277909-rn1dow26	105	8	the	the	DT
cord-277909-rn1dow26	105	9	internet	internet	NN
cord-277909-rn1dow26	105	10	,	,	,
cord-277909-rn1dow26	105	11	for	for	IN
cord-277909-rn1dow26	105	12	example	example	NN
cord-277909-rn1dow26	105	13	,	,	,
cord-277909-rn1dow26	105	14	Michael	Michael	NNP
cord-277909-rn1dow26	105	15	Zukers	Zukers	NNP
cord-277909-rn1dow26	105	16	'	'	POS
cord-277909-rn1dow26	105	17	m	m	NN
cord-277909-rn1dow26	105	18	-	-	JJ
cord-277909-rn1dow26	105	19	fold	fold	JJ
cord-277909-rn1dow26	105	20	server	server	NN
cord-277909-rn1dow26	105	21	(	(	-LRB-
cord-277909-rn1dow26	105	22	http://www.bioinfo.rpi.edu/∼zukerm/rna/	http://www.bioinfo.rpi.edu/∼zukerm/rna/	NNP
cord-277909-rn1dow26	105	23	)	)	-RRB-
cord-277909-rn1dow26	105	24	is	be	VBZ
cord-277909-rn1dow26	105	25	particularly	particularly	RB
cord-277909-rn1dow26	105	26	useful	useful	JJ
cord-277909-rn1dow26	105	27	.	.	.
cord-277909-rn1dow26	106	1	A	a	DT
cord-277909-rn1dow26	106	2	highly	highly	RB
cord-277909-rn1dow26	106	3	structured	structured	JJ
cord-277909-rn1dow26	106	4	amplicon	amplicon	NN
cord-277909-rn1dow26	106	5	(	(	-LRB-
cord-277909-rn1dow26	106	6	higher	high	JJR
cord-277909-rn1dow26	106	7	−	−	CD
cord-277909-rn1dow26	106	8	G	g	NN
cord-277909-rn1dow26	106	9	)	)	-RRB-
cord-277909-rn1dow26	106	10	may	may	MD
cord-277909-rn1dow26	106	11	reduce	reduce	VB
cord-277909-rn1dow26	106	12	the	the	DT
cord-277909-rn1dow26	106	13	efficiency	efficiency	NN
cord-277909-rn1dow26	106	14	of	of	IN
cord-277909-rn1dow26	106	15	reverse	reverse	JJ
cord-277909-rn1dow26	106	16	transcription	transcription	NN
cord-277909-rn1dow26	106	17	or	or	CC
cord-277909-rn1dow26	106	18	primer	primer	NN
cord-277909-rn1dow26	106	19	annealing	annealing	NN
cord-277909-rn1dow26	106	20	(	(	-LRB-
cord-277909-rn1dow26	106	21	Fig	Fig	NNP
cord-277909-rn1dow26	106	22	.	.	.
cord-277909-rn1dow26	106	23	5	5	CD
cord-277909-rn1dow26	106	24	)	)	-RRB-
cord-277909-rn1dow26	106	25	.	.	.
cord-277909-rn1dow26	107	1	This	this	DT
cord-277909-rn1dow26	107	2	may	may	MD
cord-277909-rn1dow26	107	3	reduce	reduce	VB
cord-277909-rn1dow26	107	4	the	the	DT
cord-277909-rn1dow26	107	5	overall	overall	JJ
cord-277909-rn1dow26	107	6	sensitivity	sensitivity	NN
cord-277909-rn1dow26	107	7	of	of	IN
cord-277909-rn1dow26	107	8	the	the	DT
cord-277909-rn1dow26	107	9	assay	assay	NN
cord-277909-rn1dow26	107	10	.	.	.
cord-277909-rn1dow26	108	1	The	the	DT
cord-277909-rn1dow26	108	2	final	final	JJ
cord-277909-rn1dow26	108	3	stage	stage	NN
cord-277909-rn1dow26	108	4	of	of	IN
cord-277909-rn1dow26	108	5	the	the	DT
cord-277909-rn1dow26	108	6	design	design	NN
cord-277909-rn1dow26	108	7	process	process	NN
cord-277909-rn1dow26	108	8	is	be	VBZ
cord-277909-rn1dow26	108	9	to	to	TO
cord-277909-rn1dow26	108	10	check	check	VB
cord-277909-rn1dow26	108	11	the	the	DT
cord-277909-rn1dow26	108	12	amplicon	amplicon	NN
cord-277909-rn1dow26	108	13	for	for	IN
cord-277909-rn1dow26	108	14	specificity	specificity	NN
cord-277909-rn1dow26	108	15	using	use	VBG
cord-277909-rn1dow26	108	16	the	the	DT
cord-277909-rn1dow26	108	17	BLAST	blast	NN
cord-277909-rn1dow26	108	18	algorithm	algorithm	NN
cord-277909-rn1dow26	108	19	(	(	-LRB-
cord-277909-rn1dow26	108	20	http://www.ncbi.nlm.nih.gov/BLAST/	http://www.ncbi.nlm.nih.gov/BLAST/	NNP
cord-277909-rn1dow26	108	21	)	)	-RRB-
cord-277909-rn1dow26	108	22	.	.	.
cord-277909-rn1dow26	109	1	The	the	DT
cord-277909-rn1dow26	109	2	assay	assay	NN
cord-277909-rn1dow26	109	3	should	should	MD
cord-277909-rn1dow26	109	4	be	be	VB
cord-277909-rn1dow26	109	5	specific	specific	JJ
cord-277909-rn1dow26	109	6	for	for	IN
cord-277909-rn1dow26	109	7	the	the	DT
cord-277909-rn1dow26	109	8	sequence	sequence	NN
cord-277909-rn1dow26	109	9	/	/	SYM
cord-277909-rn1dow26	109	10	organism	organism	NN
cord-277909-rn1dow26	109	11	of	of	IN
cord-277909-rn1dow26	109	12	interest	interest	NN
cord-277909-rn1dow26	109	13	,	,	,
cord-277909-rn1dow26	109	14	and	and	CC
cord-277909-rn1dow26	109	15	should	should	MD
cord-277909-rn1dow26	109	16	not	not	RB
cord-277909-rn1dow26	109	17	detect	detect	VB
cord-277909-rn1dow26	109	18	other	other	JJ
cord-277909-rn1dow26	109	19	sequences	sequence	NNS
cord-277909-rn1dow26	109	20	.	.	.
cord-277909-rn1dow26	110	1	Non	non	JJ
cord-277909-rn1dow26	110	2	-	-	JJ
cord-277909-rn1dow26	110	3	specific	specific	JJ
cord-277909-rn1dow26	110	4	matches	match	NNS
cord-277909-rn1dow26	110	5	may	may	MD
cord-277909-rn1dow26	110	6	be	be	VB
cord-277909-rn1dow26	110	7	picked	pick	VBN
cord-277909-rn1dow26	110	8	up	up	RP
cord-277909-rn1dow26	110	9	,	,	,
cord-277909-rn1dow26	110	10	but	but	CC
cord-277909-rn1dow26	110	11	closer	close	JJR
cord-277909-rn1dow26	110	12	analysis	analysis	NN
cord-277909-rn1dow26	110	13	of	of	IN
cord-277909-rn1dow26	110	14	the	the	DT
cord-277909-rn1dow26	110	15	primer	primer	NN
cord-277909-rn1dow26	110	16	and	and	CC
cord-277909-rn1dow26	110	17	probe	probe	VB
cord-277909-rn1dow26	110	18	binding	bind	VBG
cord-277909-rn1dow26	110	19	sites	site	NNS
cord-277909-rn1dow26	110	20	often	often	RB
cord-277909-rn1dow26	110	21	confirms	confirm	VBZ
cord-277909-rn1dow26	110	22	that	that	IN
cord-277909-rn1dow26	110	23	these	these	DT
cord-277909-rn1dow26	110	24	sequences	sequence	NNS
cord-277909-rn1dow26	110	25	will	will	MD
cord-277909-rn1dow26	110	26	not	not	RB
cord-277909-rn1dow26	110	27	be	be	VB
cord-277909-rn1dow26	110	28	amplified	amplify	VBN
cord-277909-rn1dow26	110	29	or	or	CC
cord-277909-rn1dow26	110	30	detected	detect	VBN
cord-277909-rn1dow26	110	31	due	due	IN
cord-277909-rn1dow26	110	32	to	to	IN
cord-277909-rn1dow26	110	33	multiple	multiple	JJ
cord-277909-rn1dow26	110	34	base	base	NN
cord-277909-rn1dow26	110	35	changes	change	NNS
cord-277909-rn1dow26	110	36	.	.	.
cord-277909-rn1dow26	111	1	When	when	WRB
cord-277909-rn1dow26	111	2	performing	perform	VBG
cord-277909-rn1dow26	111	3	gel	gel	NN
cord-277909-rn1dow26	111	4	based	base	VBN
cord-277909-rn1dow26	111	5	PCR	PCR	NNP
cord-277909-rn1dow26	111	6	it	-PRON-	PRP
cord-277909-rn1dow26	111	7	is	be	VBZ
cord-277909-rn1dow26	111	8	essential	essential	JJ
cord-277909-rn1dow26	111	9	to	to	TO
cord-277909-rn1dow26	111	10	fully	fully	RB
cord-277909-rn1dow26	111	11	optimise	optimise	VB
cord-277909-rn1dow26	111	12	primer	primer	NN
cord-277909-rn1dow26	111	13	concentrations	concentration	NNS
cord-277909-rn1dow26	111	14	to	to	TO
cord-277909-rn1dow26	111	15	achieve	achieve	VB
cord-277909-rn1dow26	111	16	the	the	DT
cord-277909-rn1dow26	111	17	best	good	JJS
cord-277909-rn1dow26	111	18	sensitivity	sensitivity	NN
cord-277909-rn1dow26	111	19	of	of	IN
cord-277909-rn1dow26	111	20	the	the	DT
cord-277909-rn1dow26	111	21	assay	assay	NN
cord-277909-rn1dow26	111	22	and	and	CC
cord-277909-rn1dow26	111	23	best	good	JJS
cord-277909-rn1dow26	111	24	end	end	NN
cord-277909-rn1dow26	111	25	-	-	HYPH
cord-277909-rn1dow26	111	26	point	point	NN
cord-277909-rn1dow26	111	27	signal	signal	NN
cord-277909-rn1dow26	111	28	(	(	-LRB-
cord-277909-rn1dow26	111	29	brightness	brightness	NN
cord-277909-rn1dow26	111	30	of	of	IN
cord-277909-rn1dow26	111	31	band	band	NN
cord-277909-rn1dow26	111	32	)	)	-RRB-
cord-277909-rn1dow26	112	1	(	(	-LRB-
cord-277909-rn1dow26	112	2	Gunson	Gunson	NNP
cord-277909-rn1dow26	112	3	et	et	FW
cord-277909-rn1dow26	112	4	al	al	NNP
cord-277909-rn1dow26	112	5	.	.	NNP
cord-277909-rn1dow26	112	6	,	,	,
cord-277909-rn1dow26	112	7	2003	2003	CD
cord-277909-rn1dow26	112	8	)	)	-RRB-
cord-277909-rn1dow26	112	9	.	.	.
cord-277909-rn1dow26	113	1	In	in	IN
cord-277909-rn1dow26	113	2	real	real	JJ
cord-277909-rn1dow26	113	3	time	time	NN
cord-277909-rn1dow26	113	4	PCR	PCR	NNP
cord-277909-rn1dow26	113	5	,	,	,
cord-277909-rn1dow26	113	6	the	the	DT
cord-277909-rn1dow26	113	7	signal	signal	NN
cord-277909-rn1dow26	113	8	is	be	VBZ
cord-277909-rn1dow26	113	9	detected	detect	VBN
cord-277909-rn1dow26	113	10	early	early	RB
cord-277909-rn1dow26	113	11	in	in	IN
cord-277909-rn1dow26	113	12	the	the	DT
cord-277909-rn1dow26	113	13	amplification	amplification	NN
cord-277909-rn1dow26	113	14	process	process	NN
cord-277909-rn1dow26	113	15	,	,	,
cord-277909-rn1dow26	113	16	and	and	CC
cord-277909-rn1dow26	113	17	therefore	therefore	RB
cord-277909-rn1dow26	113	18	the	the	DT
cord-277909-rn1dow26	113	19	end	end	NN
cord-277909-rn1dow26	113	20	-	-	HYPH
cord-277909-rn1dow26	113	21	point	point	NN
cord-277909-rn1dow26	113	22	variation	variation	NN
cord-277909-rn1dow26	113	23	seen	see	VBN
cord-277909-rn1dow26	113	24	in	in	IN
cord-277909-rn1dow26	113	25	gel	gel	NN
cord-277909-rn1dow26	113	26	-	-	HYPH
cord-277909-rn1dow26	113	27	based	base	VBN
cord-277909-rn1dow26	113	28	assays	assay	NNS
cord-277909-rn1dow26	113	29	does	do	VBZ
cord-277909-rn1dow26	113	30	not	not	RB
cord-277909-rn1dow26	113	31	affect	affect	VB
cord-277909-rn1dow26	113	32	the	the	DT
cord-277909-rn1dow26	113	33	result	result	NN
cord-277909-rn1dow26	113	34	.	.	.
cord-277909-rn1dow26	114	1	Also	also	RB
cord-277909-rn1dow26	114	2	,	,	,
cord-277909-rn1dow26	114	3	careful	careful	JJ
cord-277909-rn1dow26	114	4	design	design	NN
cord-277909-rn1dow26	114	5	of	of	IN
cord-277909-rn1dow26	114	6	the	the	DT
cord-277909-rn1dow26	114	7	assay	assay	NN
cord-277909-rn1dow26	114	8	can	can	MD
cord-277909-rn1dow26	114	9	reduce	reduce	VB
cord-277909-rn1dow26	114	10	primer	primer	NN
cord-277909-rn1dow26	114	11	-	-	HYPH
cord-277909-rn1dow26	114	12	dimer	dimer	NN
cord-277909-rn1dow26	114	13	formation	formation	NN
cord-277909-rn1dow26	114	14	Fig	Fig	NNP
cord-277909-rn1dow26	114	15	.	.	.
cord-277909-rn1dow26	115	1	5	5	LS
cord-277909-rn1dow26	115	2	.	.	.
cord-277909-rn1dow26	116	1	Structure	structure	NN
cord-277909-rn1dow26	116	2	within	within	IN
cord-277909-rn1dow26	116	3	PCR	PCR	NNP
cord-277909-rn1dow26	116	4	amplicons	amplicon	NNS
cord-277909-rn1dow26	116	5	may	may	MD
cord-277909-rn1dow26	116	6	affect	affect	VB
cord-277909-rn1dow26	116	7	the	the	DT
cord-277909-rn1dow26	116	8	sensitivity	sensitivity	NN
cord-277909-rn1dow26	116	9	of	of	IN
cord-277909-rn1dow26	116	10	an	an	DT
cord-277909-rn1dow26	116	11	assay	assay	NN
cord-277909-rn1dow26	116	12	.	.	.
cord-277909-rn1dow26	117	1	Respiratory	respiratory	JJ
cord-277909-rn1dow26	117	2	syncitial	syncitial	JJ
cord-277909-rn1dow26	117	3	virus	virus	NN
cord-277909-rn1dow26	117	4	(	(	-LRB-
cord-277909-rn1dow26	117	5	RSV)-A	RSV)-A	NNP
cord-277909-rn1dow26	117	6	detection	detection	NN
cord-277909-rn1dow26	117	7	limit	limit	NN
cord-277909-rn1dow26	117	8	is	be	VBZ
cord-277909-rn1dow26	117	9	10	10	CD
cord-277909-rn1dow26	117	10	2	2	CD
cord-277909-rn1dow26	117	11	copies	copy	NNS
cord-277909-rn1dow26	117	12	/	/	SYM
cord-277909-rn1dow26	117	13	reaction	reaction	NN
cord-277909-rn1dow26	117	14	,	,	,
cord-277909-rn1dow26	117	15	while	while	IN
cord-277909-rn1dow26	117	16	RSV	RSV	NNP
cord-277909-rn1dow26	117	17	-	-	HYPH
cord-277909-rn1dow26	117	18	B	B	NNP
cord-277909-rn1dow26	117	19	which	which	WDT
cord-277909-rn1dow26	117	20	is	be	VBZ
cord-277909-rn1dow26	117	21	more	more	RBR
cord-277909-rn1dow26	117	22	structured	structured	JJ
cord-277909-rn1dow26	117	23	has	have	VBZ
cord-277909-rn1dow26	117	24	a	a	DT
cord-277909-rn1dow26	117	25	detection	detection	NN
cord-277909-rn1dow26	117	26	limit	limit	NN
cord-277909-rn1dow26	117	27	of	of	IN
cord-277909-rn1dow26	117	28	10	10	CD
cord-277909-rn1dow26	117	29	4	4	CD
cord-277909-rn1dow26	117	30	-10	-10	CD
cord-277909-rn1dow26	117	31	5	5	CD
cord-277909-rn1dow26	117	32	copies	copy	NNS
cord-277909-rn1dow26	117	33	per	per	IN
cord-277909-rn1dow26	117	34	reaction	reaction	NN
cord-277909-rn1dow26	117	35	.	.	.
cord-277909-rn1dow26	118	1	and	and	CC
cord-277909-rn1dow26	118	2	increase	increase	VB
cord-277909-rn1dow26	118	3	the	the	DT
cord-277909-rn1dow26	118	4	efficiency	efficiency	NN
cord-277909-rn1dow26	118	5	of	of	IN
cord-277909-rn1dow26	118	6	the	the	DT
cord-277909-rn1dow26	118	7	specific	specific	JJ
cord-277909-rn1dow26	118	8	amplification	amplification	NN
cord-277909-rn1dow26	118	9	reaction	reaction	NN
cord-277909-rn1dow26	118	10	.	.	.
cord-277909-rn1dow26	119	1	Consequently	consequently	RB
cord-277909-rn1dow26	119	2	,	,	,
cord-277909-rn1dow26	119	3	many	many	JJ
cord-277909-rn1dow26	119	4	manufacturers	manufacturer	NNS
cord-277909-rn1dow26	119	5	of	of	IN
cord-277909-rn1dow26	119	6	real	real	JJ
cord-277909-rn1dow26	119	7	time	time	NN
cord-277909-rn1dow26	119	8	PCR	pcr	NN
cord-277909-rn1dow26	119	9	equipment	equipment	NN
cord-277909-rn1dow26	119	10	and	and	CC
cord-277909-rn1dow26	119	11	oligonucleotide	oligonucleotide	NN
cord-277909-rn1dow26	119	12	primers	primer	NNS
cord-277909-rn1dow26	119	13	and	and	CC
cord-277909-rn1dow26	119	14	probes	probe	NNS
cord-277909-rn1dow26	119	15	no	no	RB
cord-277909-rn1dow26	119	16	longer	longer	RB
cord-277909-rn1dow26	119	17	recommend	recommend	VB
cord-277909-rn1dow26	119	18	optimising	optimise	VBG
cord-277909-rn1dow26	119	19	primer	primer	NN
cord-277909-rn1dow26	119	20	and	and	CC
cord-277909-rn1dow26	119	21	probe	probe	VB
cord-277909-rn1dow26	119	22	concentrations	concentration	NNS
cord-277909-rn1dow26	119	23	for	for	IN
cord-277909-rn1dow26	119	24	real	real	JJ
cord-277909-rn1dow26	119	25	time	time	NN
cord-277909-rn1dow26	119	26	Taqman	Taqman	NNP
cord-277909-rn1dow26	119	27	assays	assay	NNS
cord-277909-rn1dow26	119	28	.	.	.
cord-277909-rn1dow26	120	1	Despite	despite	IN
cord-277909-rn1dow26	120	2	this	this	DT
cord-277909-rn1dow26	120	3	we	-PRON-	PRP
cord-277909-rn1dow26	120	4	still	still	RB
cord-277909-rn1dow26	120	5	perform	perform	VBP
cord-277909-rn1dow26	120	6	an	an	DT
cord-277909-rn1dow26	120	7	initial	initial	JJ
cord-277909-rn1dow26	120	8	optimisation	optimisation	NN
cord-277909-rn1dow26	120	9	of	of	IN
cord-277909-rn1dow26	120	10	both	both	DT
cord-277909-rn1dow26	120	11	primer	primer	NN
cord-277909-rn1dow26	120	12	and	and	CC
cord-277909-rn1dow26	120	13	probe	probe	VB
cord-277909-rn1dow26	120	14	concentrations	concentration	NNS
cord-277909-rn1dow26	120	15	to	to	TO
cord-277909-rn1dow26	120	16	ensure	ensure	VB
cord-277909-rn1dow26	120	17	we	-PRON-	PRP
cord-277909-rn1dow26	120	18	are	be	VBP
cord-277909-rn1dow26	120	19	running	run	VBG
cord-277909-rn1dow26	120	20	our	-PRON-	PRP$
cord-277909-rn1dow26	120	21	real	real	JJ
cord-277909-rn1dow26	120	22	time	time	NN
cord-277909-rn1dow26	120	23	PCR	pcr	NN
cord-277909-rn1dow26	120	24	assays	assay	NNS
cord-277909-rn1dow26	120	25	at	at	IN
cord-277909-rn1dow26	120	26	their	-PRON-	PRP$
cord-277909-rn1dow26	120	27	most	most	RBS
cord-277909-rn1dow26	120	28	sensitive	sensitive	JJ
cord-277909-rn1dow26	120	29	and	and	CC
cord-277909-rn1dow26	120	30	efficient	efficient	JJ
cord-277909-rn1dow26	120	31	.	.	.
cord-277909-rn1dow26	121	1	Although	although	IN
cord-277909-rn1dow26	121	2	for	for	IN
cord-277909-rn1dow26	121	3	the	the	DT
cord-277909-rn1dow26	121	4	majority	majority	NN
cord-277909-rn1dow26	121	5	of	of	IN
cord-277909-rn1dow26	121	6	our	-PRON-	PRP$
cord-277909-rn1dow26	121	7	assays	assay	NNS
cord-277909-rn1dow26	121	8	the	the	DT
cord-277909-rn1dow26	121	9	optimal	optimal	JJ
cord-277909-rn1dow26	121	10	concentrations	concentration	NNS
cord-277909-rn1dow26	121	11	are	be	VBP
cord-277909-rn1dow26	121	12	1000:1000:300	1000:1000:300	CD
cord-277909-rn1dow26	121	13	nM	nm	CD
cord-277909-rn1dow26	121	14	(	(	-LRB-
cord-277909-rn1dow26	121	15	forward	forward	RB
cord-277909-rn1dow26	121	16	:	:	:
cord-277909-rn1dow26	121	17	reverse	reverse	VB
cord-277909-rn1dow26	121	18	:	:	:
cord-277909-rn1dow26	121	19	probe	probe	NN
cord-277909-rn1dow26	121	20	)	)	-RRB-
cord-277909-rn1dow26	121	21	,	,	,
cord-277909-rn1dow26	121	22	we	-PRON-	PRP
cord-277909-rn1dow26	121	23	have	have	VBP
cord-277909-rn1dow26	121	24	observed	observe	VBN
cord-277909-rn1dow26	121	25	on	on	IN
cord-277909-rn1dow26	121	26	several	several	JJ
cord-277909-rn1dow26	121	27	occasions	occasion	NNS
cord-277909-rn1dow26	121	28	that	that	IN
cord-277909-rn1dow26	121	29	the	the	DT
cord-277909-rn1dow26	121	30	optimal	optimal	JJ
cord-277909-rn1dow26	121	31	primer	primer	NN
cord-277909-rn1dow26	121	32	and	and	CC
cord-277909-rn1dow26	121	33	probe	probe	NN
cord-277909-rn1dow26	121	34	concentrations	concentration	NNS
cord-277909-rn1dow26	121	35	were	be	VBD
cord-277909-rn1dow26	121	36	different	different	JJ
cord-277909-rn1dow26	121	37	to	to	IN
cord-277909-rn1dow26	121	38	the	the	DT
cord-277909-rn1dow26	121	39	values	value	NNS
cord-277909-rn1dow26	121	40	recommended	recommend	VBN
cord-277909-rn1dow26	121	41	.	.	.
cord-277909-rn1dow26	122	1	Our	-PRON-	PRP$
cord-277909-rn1dow26	122	2	method	method	NN
cord-277909-rn1dow26	122	3	for	for	IN
cord-277909-rn1dow26	122	4	primer	primer	NN
cord-277909-rn1dow26	122	5	and	and	CC
cord-277909-rn1dow26	122	6	probe	probe	NN
cord-277909-rn1dow26	122	7	optimisation	optimisation	NN
cord-277909-rn1dow26	122	8	is	be	VBZ
cord-277909-rn1dow26	122	9	available	available	JJ
cord-277909-rn1dow26	122	10	online	online	RB
cord-277909-rn1dow26	122	11	(	(	-LRB-
cord-277909-rn1dow26	122	12	www.clinical-virology.org	www.clinical-virology.org	NNP
cord-277909-rn1dow26	122	13	)	)	-RRB-
cord-277909-rn1dow26	122	14	.	.	.
cord-277909-rn1dow26	123	1	However	however	RB
cord-277909-rn1dow26	123	2	,	,	,
cord-277909-rn1dow26	123	3	other	other	JJ
cord-277909-rn1dow26	123	4	methods	method	NNS
cord-277909-rn1dow26	123	5	will	will	MD
cord-277909-rn1dow26	123	6	also	also	RB
cord-277909-rn1dow26	123	7	be	be	VB
cord-277909-rn1dow26	123	8	available	available	JJ
cord-277909-rn1dow26	123	9	.	.	.
cord-277909-rn1dow26	124	1	Optimisation	optimisation	NN
cord-277909-rn1dow26	124	2	of	of	IN
cord-277909-rn1dow26	124	3	a	a	DT
cord-277909-rn1dow26	124	4	real	real	JJ
cord-277909-rn1dow26	124	5	time	time	NN
cord-277909-rn1dow26	124	6	PCR	PCR	NNP
cord-277909-rn1dow26	124	7	requires	require	VBZ
cord-277909-rn1dow26	124	8	positive	positive	JJ
cord-277909-rn1dow26	124	9	control	control	NN
cord-277909-rn1dow26	124	10	material	material	NN
cord-277909-rn1dow26	124	11	.	.	.
cord-277909-rn1dow26	125	1	Where	where	WRB
cord-277909-rn1dow26	125	2	positive	positive	JJ
cord-277909-rn1dow26	125	3	control	control	NN
cord-277909-rn1dow26	125	4	is	be	VBZ
cord-277909-rn1dow26	125	5	not	not	RB
cord-277909-rn1dow26	125	6	available	available	JJ
cord-277909-rn1dow26	125	7	(	(	-LRB-
cord-277909-rn1dow26	125	8	examples	example	NNS
cord-277909-rn1dow26	125	9	being	be	VBG
cord-277909-rn1dow26	125	10	a	a	DT
cord-277909-rn1dow26	125	11	virus	virus	NN
cord-277909-rn1dow26	125	12	,	,	,
cord-277909-rn1dow26	125	13	which	which	WDT
cord-277909-rn1dow26	125	14	can	can	MD
cord-277909-rn1dow26	125	15	not	not	RB
cord-277909-rn1dow26	125	16	be	be	VB
cord-277909-rn1dow26	125	17	cultured	cultured	JJ
cord-277909-rn1dow26	125	18	or	or	CC
cord-277909-rn1dow26	125	19	a	a	DT
cord-277909-rn1dow26	125	20	highly	highly	RB
cord-277909-rn1dow26	125	21	pathogenic	pathogenic	JJ
cord-277909-rn1dow26	125	22	virus	virus	NN
cord-277909-rn1dow26	125	23	such	such	JJ
cord-277909-rn1dow26	125	24	as	as	IN
cord-277909-rn1dow26	125	25	H5	H5	NNP
cord-277909-rn1dow26	125	26	influenza	influenza	NN
cord-277909-rn1dow26	125	27	or	or	CC
cord-277909-rn1dow26	125	28	SARS	SARS	NNP
cord-277909-rn1dow26	125	29	coronavirus	coronavirus	NN
cord-277909-rn1dow26	125	30	)	)	-RRB-
cord-277909-rn1dow26	125	31	,	,	,
cord-277909-rn1dow26	125	32	DNA	DNA	NNP
cord-277909-rn1dow26	125	33	or	or	CC
cord-277909-rn1dow26	125	34	RNA	RNA	NNP
cord-277909-rn1dow26	125	35	oligonucleotide	oligonucleotide	NN
cord-277909-rn1dow26	125	36	targets	target	NNS
cord-277909-rn1dow26	125	37	may	may	MD
cord-277909-rn1dow26	125	38	be	be	VB
cord-277909-rn1dow26	125	39	ordered	order	VBN
cord-277909-rn1dow26	125	40	.	.	.
cord-277909-rn1dow26	126	1	These	these	DT
cord-277909-rn1dow26	126	2	are	be	VBP
cord-277909-rn1dow26	126	3	also	also	RB
cord-277909-rn1dow26	126	4	useful	useful	JJ
cord-277909-rn1dow26	126	5	as	as	IN
cord-277909-rn1dow26	126	6	alternatives	alternative	NNS
cord-277909-rn1dow26	126	7	to	to	IN
cord-277909-rn1dow26	126	8	plasmids	plasmid	NNS
cord-277909-rn1dow26	126	9	as	as	IN
cord-277909-rn1dow26	126	10	standards	standard	NNS
cord-277909-rn1dow26	126	11	in	in	IN
cord-277909-rn1dow26	126	12	quantitative	quantitative	JJ
cord-277909-rn1dow26	126	13	assays	assay	NNS
cord-277909-rn1dow26	126	14	.	.	.
cord-277909-rn1dow26	127	1	It	-PRON-	PRP
cord-277909-rn1dow26	127	2	should	should	MD
cord-277909-rn1dow26	127	3	be	be	VB
cord-277909-rn1dow26	127	4	noted	note	VBN
cord-277909-rn1dow26	127	5	that	that	IN
cord-277909-rn1dow26	127	6	these	these	DT
cord-277909-rn1dow26	127	7	oligonucleotide	oligonucleotide	JJ
cord-277909-rn1dow26	127	8	controls	control	NNS
cord-277909-rn1dow26	127	9	must	must	MD
cord-277909-rn1dow26	127	10	be	be	VB
cord-277909-rn1dow26	127	11	ordered	order	VBN
cord-277909-rn1dow26	127	12	from	from	IN
cord-277909-rn1dow26	127	13	a	a	DT
cord-277909-rn1dow26	127	14	separate	separate	JJ
cord-277909-rn1dow26	127	15	supplier	supplier	NN
cord-277909-rn1dow26	127	16	to	to	TO
cord-277909-rn1dow26	127	17	prevent	prevent	VB
cord-277909-rn1dow26	127	18	contamination	contamination	NN
cord-277909-rn1dow26	127	19	of	of	IN
cord-277909-rn1dow26	127	20	the	the	DT
cord-277909-rn1dow26	127	21	primer	primer	NN
cord-277909-rn1dow26	127	22	-	-	HYPH
cord-277909-rn1dow26	127	23	probe	probe	NN
cord-277909-rn1dow26	127	24	set	set	NN
cord-277909-rn1dow26	127	25	,	,	,
cord-277909-rn1dow26	127	26	and	and	CC
cord-277909-rn1dow26	127	27	should	should	MD
cord-277909-rn1dow26	127	28	be	be	VB
cord-277909-rn1dow26	127	29	diluted	dilute	VBN
cord-277909-rn1dow26	127	30	in	in	IN
cord-277909-rn1dow26	127	31	a	a	DT
cord-277909-rn1dow26	127	32	separate	separate	JJ
cord-277909-rn1dow26	127	33	laboratory	laboratory	NN
cord-277909-rn1dow26	127	34	prior	prior	RB
cord-277909-rn1dow26	127	35	to	to	TO
cord-277909-rn1dow26	127	36	use	use	NN
cord-277909-rn1dow26	127	37	as	as	IN
cord-277909-rn1dow26	127	38	they	-PRON-	PRP
cord-277909-rn1dow26	127	39	may	may	MD
cord-277909-rn1dow26	127	40	contain	contain	VB
cord-277909-rn1dow26	127	41	up	up	IN
cord-277909-rn1dow26	127	42	to	to	TO
cord-277909-rn1dow26	127	43	10	10	CD
cord-277909-rn1dow26	127	44	17	17	CD
cord-277909-rn1dow26	127	45	target	target	NN
cord-277909-rn1dow26	127	46	copies	copy	NNS
cord-277909-rn1dow26	127	47	per	per	IN
cord-277909-rn1dow26	127	48	ml	ml	NN
cord-277909-rn1dow26	127	49	,	,	,
cord-277909-rn1dow26	127	50	and	and	CC
cord-277909-rn1dow26	127	51	are	be	VBP
cord-277909-rn1dow26	127	52	therefore	therefore	RB
cord-277909-rn1dow26	127	53	a	a	DT
cord-277909-rn1dow26	127	54	considerable	considerable	JJ
cord-277909-rn1dow26	127	55	source	source	NN
cord-277909-rn1dow26	127	56	of	of	IN
cord-277909-rn1dow26	127	57	potential	potential	JJ
cord-277909-rn1dow26	127	58	contamination	contamination	NN
cord-277909-rn1dow26	127	59	.	.	.
cord-277909-rn1dow26	128	1	We	-PRON-	PRP
cord-277909-rn1dow26	128	2	have	have	VBP
cord-277909-rn1dow26	128	3	observed	observe	VBN
cord-277909-rn1dow26	128	4	contamination	contamination	NN
cord-277909-rn1dow26	128	5	in	in	IN
cord-277909-rn1dow26	128	6	erythrovirus	erythrovirus	NNP
cord-277909-rn1dow26	128	7	B19	B19	NNP
cord-277909-rn1dow26	128	8	primers	primer	NNS
cord-277909-rn1dow26	128	9	purchased	purchase	VBD
cord-277909-rn1dow26	128	10	several	several	JJ
cord-277909-rn1dow26	128	11	months	month	NNS
cord-277909-rn1dow26	128	12	after	after	IN
cord-277909-rn1dow26	128	13	a	a	DT
cord-277909-rn1dow26	128	14	full	full	JJ
cord-277909-rn1dow26	128	15	length	length	NN
cord-277909-rn1dow26	128	16	oligonucleotide	oligonucleotide	JJ
cord-277909-rn1dow26	128	17	control	control	NN
cord-277909-rn1dow26	128	18	was	be	VBD
cord-277909-rn1dow26	128	19	ordered	order	VBN
cord-277909-rn1dow26	128	20	from	from	IN
cord-277909-rn1dow26	128	21	the	the	DT
cord-277909-rn1dow26	128	22	same	same	JJ
cord-277909-rn1dow26	128	23	supplier	supplier	NN
cord-277909-rn1dow26	128	24	(	(	-LRB-
cord-277909-rn1dow26	128	25	Fig	Fig	NNP
cord-277909-rn1dow26	128	26	.	.	.
cord-277909-rn1dow26	128	27	6	6	CD
cord-277909-rn1dow26	128	28	)	)	-RRB-
cord-277909-rn1dow26	128	29	.	.	.
cord-277909-rn1dow26	129	1	Once	once	RB
cord-277909-rn1dow26	129	2	the	the	DT
cord-277909-rn1dow26	129	3	assay	assay	NN
cord-277909-rn1dow26	129	4	is	be	VBZ
cord-277909-rn1dow26	129	5	optimised	optimise	VBN
cord-277909-rn1dow26	129	6	,	,	,
cord-277909-rn1dow26	129	7	it	-PRON-	PRP
cord-277909-rn1dow26	129	8	is	be	VBZ
cord-277909-rn1dow26	129	9	essential	essential	JJ
cord-277909-rn1dow26	129	10	to	to	TO
cord-277909-rn1dow26	129	11	check	check	VB
cord-277909-rn1dow26	129	12	the	the	DT
cord-277909-rn1dow26	129	13	sensitivity	sensitivity	NN
cord-277909-rn1dow26	129	14	and	and	CC
cord-277909-rn1dow26	129	15	specificity	specificity	NN
cord-277909-rn1dow26	129	16	of	of	IN
cord-277909-rn1dow26	129	17	a	a	DT
cord-277909-rn1dow26	129	18	new	new	JJ
cord-277909-rn1dow26	129	19	PCR	PCR	NNP
cord-277909-rn1dow26	129	20	assay	assay	NN
cord-277909-rn1dow26	129	21	by	by	IN
cord-277909-rn1dow26	129	22	using	use	VBG
cord-277909-rn1dow26	129	23	a	a	DT
cord-277909-rn1dow26	129	24	selection	selection	NN
cord-277909-rn1dow26	129	25	of	of	IN
cord-277909-rn1dow26	129	26	sample	sample	NN
cord-277909-rn1dow26	129	27	'	'	``
cord-277909-rn1dow26	129	28	panels	panel	NNS
cord-277909-rn1dow26	129	29	'	'	''
cord-277909-rn1dow26	129	30	.	.	.
cord-277909-rn1dow26	130	1	There	there	EX
cord-277909-rn1dow26	130	2	is	be	VBZ
cord-277909-rn1dow26	130	3	much	much	JJ
cord-277909-rn1dow26	130	4	debate	debate	NN
cord-277909-rn1dow26	130	5	about	about	IN
cord-277909-rn1dow26	130	6	what	what	WP
cord-277909-rn1dow26	130	7	is	be	VBZ
cord-277909-rn1dow26	130	8	an	an	DT
cord-277909-rn1dow26	130	9	acceptable	acceptable	JJ
cord-277909-rn1dow26	130	10	validation	validation	NN
cord-277909-rn1dow26	130	11	process	process	NN
cord-277909-rn1dow26	130	12	.	.	.
cord-277909-rn1dow26	131	1	These	these	DT
cord-277909-rn1dow26	131	2	should	should	MD
cord-277909-rn1dow26	131	3	minimally	minimally	RB
cord-277909-rn1dow26	131	4	include	include	VB
cord-277909-rn1dow26	131	5	clinical	clinical	JJ
cord-277909-rn1dow26	131	6	samples	sample	NNS
cord-277909-rn1dow26	131	7	known	know	VBN
cord-277909-rn1dow26	131	8	to	to	TO
cord-277909-rn1dow26	131	9	be	be	VB
cord-277909-rn1dow26	131	10	positive	positive	JJ
cord-277909-rn1dow26	131	11	by	by	IN
cord-277909-rn1dow26	131	12	the	the	DT
cord-277909-rn1dow26	131	13	current	current	JJ
cord-277909-rn1dow26	131	14	standard	standard	JJ
cord-277909-rn1dow26	131	15	assay	assay	NN
cord-277909-rn1dow26	131	16	and	and	CC
cord-277909-rn1dow26	131	17	should	should	MD
cord-277909-rn1dow26	131	18	consist	consist	VB
cord-277909-rn1dow26	131	19	of	of	IN
cord-277909-rn1dow26	131	20	the	the	DT
cord-277909-rn1dow26	131	21	sample	sample	NN
cord-277909-rn1dow26	131	22	types	type	NNS
cord-277909-rn1dow26	131	23	commonly	commonly	RB
cord-277909-rn1dow26	131	24	submitted	submit	VBN
cord-277909-rn1dow26	131	25	to	to	TO
cord-277909-rn1dow26	131	26	be	be	VB
cord-277909-rn1dow26	131	27	examined	examine	VBN
cord-277909-rn1dow26	131	28	for	for	IN
cord-277909-rn1dow26	131	29	the	the	DT
cord-277909-rn1dow26	131	30	virus	virus	NN
cord-277909-rn1dow26	131	31	in	in	IN
cord-277909-rn1dow26	131	32	question	question	NN
cord-277909-rn1dow26	131	33	.	.	.
cord-277909-rn1dow26	132	1	Clinical	clinical	JJ
cord-277909-rn1dow26	132	2	samples	sample	NNS
cord-277909-rn1dow26	132	3	tested	test	VBD
cord-277909-rn1dow26	132	4	negative	negative	JJ
cord-277909-rn1dow26	132	5	by	by	IN
cord-277909-rn1dow26	132	6	the	the	DT
cord-277909-rn1dow26	132	7	previous	previous	JJ
cord-277909-rn1dow26	132	8	method	method	NN
cord-277909-rn1dow26	132	9	should	should	MD
cord-277909-rn1dow26	132	10	also	also	RB
cord-277909-rn1dow26	132	11	be	be	VB
cord-277909-rn1dow26	132	12	examined	examine	VBN
cord-277909-rn1dow26	132	13	to	to	TO
cord-277909-rn1dow26	132	14	determine	determine	VB
cord-277909-rn1dow26	132	15	if	if	IN
cord-277909-rn1dow26	132	16	the	the	DT
cord-277909-rn1dow26	132	17	new	new	JJ
cord-277909-rn1dow26	132	18	assay	assay	NN
cord-277909-rn1dow26	132	19	is	be	VBZ
cord-277909-rn1dow26	132	20	more	more	RBR
cord-277909-rn1dow26	132	21	sensitive	sensitive	JJ
cord-277909-rn1dow26	132	22	than	than	IN
cord-277909-rn1dow26	132	23	the	the	DT
cord-277909-rn1dow26	132	24	current	current	JJ
cord-277909-rn1dow26	132	25	test	test	NN
cord-277909-rn1dow26	132	26	,	,	,
cord-277909-rn1dow26	132	27	and	and	CC
cord-277909-rn1dow26	132	28	samples	sample	NNS
cord-277909-rn1dow26	132	29	known	know	VBN
cord-277909-rn1dow26	132	30	to	to	TO
cord-277909-rn1dow26	132	31	be	be	VB
cord-277909-rn1dow26	132	32	positive	positive	JJ
cord-277909-rn1dow26	132	33	for	for	IN
cord-277909-rn1dow26	132	34	other	other	JJ
cord-277909-rn1dow26	132	35	agents	agent	NNS
cord-277909-rn1dow26	132	36	should	should	MD
cord-277909-rn1dow26	132	37	be	be	VB
cord-277909-rn1dow26	132	38	tested	test	VBN
cord-277909-rn1dow26	132	39	to	to	TO
cord-277909-rn1dow26	132	40	confirm	confirm	VB
cord-277909-rn1dow26	132	41	assay	assay	NN
cord-277909-rn1dow26	132	42	Fig	Fig	NNP
cord-277909-rn1dow26	132	43	.	.	.
cord-277909-rn1dow26	132	44	6	6	CD
cord-277909-rn1dow26	132	45	.	.	.
cord-277909-rn1dow26	133	1	Contamination	contamination	NN
cord-277909-rn1dow26	133	2	of	of	IN
cord-277909-rn1dow26	133	3	primer	primer	NN
cord-277909-rn1dow26	133	4	and	and	CC
cord-277909-rn1dow26	133	5	probes	probe	NNS
cord-277909-rn1dow26	133	6	with	with	IN
cord-277909-rn1dow26	133	7	assay	assay	NN
cord-277909-rn1dow26	133	8	target	target	NN
cord-277909-rn1dow26	133	9	produced	produce	VBN
cord-277909-rn1dow26	133	10	at	at	IN
cord-277909-rn1dow26	133	11	the	the	DT
cord-277909-rn1dow26	133	12	same	same	JJ
cord-277909-rn1dow26	133	13	facility	facility	NN
cord-277909-rn1dow26	133	14	.	.	.
cord-277909-rn1dow26	134	1	Label	Label	NNP
cord-277909-rn1dow26	134	2	1	1	CD
cord-277909-rn1dow26	134	3	,	,	,
cord-277909-rn1dow26	134	4	reaction	reaction	NN
cord-277909-rn1dow26	134	5	component	component	NN
cord-277909-rn1dow26	134	6	:	:	:
cord-277909-rn1dow26	135	1	supplier	supplier	NN
cord-277909-rn1dow26	135	2	A	a	DT
cord-277909-rn1dow26	135	3	salt	salt	NN
cord-277909-rn1dow26	135	4	free	free	JJ
cord-277909-rn1dow26	135	5	negative	negative	JJ
cord-277909-rn1dow26	135	6	control	control	NN
cord-277909-rn1dow26	135	7	,	,	,
cord-277909-rn1dow26	135	8	mean	mean	VB
cord-277909-rn1dow26	135	9	C	C	NNP
cord-277909-rn1dow26	135	10	T	t	NN
cord-277909-rn1dow26	135	11	value	value	NN
cord-277909-rn1dow26	135	12	:	:	:
cord-277909-rn1dow26	135	13	21.08	21.08	CD
cord-277909-rn1dow26	135	14	;	;	:
cord-277909-rn1dow26	135	15	label	label	NNP
cord-277909-rn1dow26	135	16	2	2	CD
cord-277909-rn1dow26	135	17	,	,	,
cord-277909-rn1dow26	135	18	reaction	reaction	NN
cord-277909-rn1dow26	135	19	component	component	NN
cord-277909-rn1dow26	135	20	:	:	:
cord-277909-rn1dow26	135	21	supplier	supplier	NN
cord-277909-rn1dow26	135	22	A	a	DT
cord-277909-rn1dow26	135	23	salt	salt	NN
cord-277909-rn1dow26	135	24	free	free	JJ
cord-277909-rn1dow26	135	25	positive	positive	JJ
cord-277909-rn1dow26	135	26	control	control	NN
cord-277909-rn1dow26	135	27	(	(	-LRB-
cord-277909-rn1dow26	135	28	−7	−7	NNP
cord-277909-rn1dow26	135	29	)	)	-RRB-
cord-277909-rn1dow26	136	1	,	,	,
cord-277909-rn1dow26	136	2	mean	mean	VB
cord-277909-rn1dow26	136	3	C	C	NNP
cord-277909-rn1dow26	136	4	T	t	NN
cord-277909-rn1dow26	136	5	value	value	NN
cord-277909-rn1dow26	136	6	:	:	:
cord-277909-rn1dow26	136	7	21.10	21.10	CD
cord-277909-rn1dow26	136	8	;	;	:
cord-277909-rn1dow26	136	9	label	label	NNP
cord-277909-rn1dow26	136	10	3	3	CD
cord-277909-rn1dow26	136	11	,	,	,
cord-277909-rn1dow26	136	12	reaction	reaction	NN
cord-277909-rn1dow26	136	13	component	component	NN
cord-277909-rn1dow26	136	14	:	:	:
cord-277909-rn1dow26	137	1	supplier	supplier	NN
cord-277909-rn1dow26	137	2	A	a	DT
cord-277909-rn1dow26	137	3	HPLC	HPLC	NNP
cord-277909-rn1dow26	137	4	negative	negative	JJ
cord-277909-rn1dow26	137	5	control	control	NN
cord-277909-rn1dow26	137	6	,	,	,
cord-277909-rn1dow26	137	7	mean	mean	VB
cord-277909-rn1dow26	137	8	C	C	NNP
cord-277909-rn1dow26	137	9	T	t	NN
cord-277909-rn1dow26	137	10	value	value	NN
cord-277909-rn1dow26	137	11	:	:	:
cord-277909-rn1dow26	137	12	33.28	33.28	CD
cord-277909-rn1dow26	137	13	;	;	:
cord-277909-rn1dow26	137	14	label	label	NNP
cord-277909-rn1dow26	137	15	4	4	CD
cord-277909-rn1dow26	137	16	,	,	,
cord-277909-rn1dow26	137	17	reaction	reaction	NN
cord-277909-rn1dow26	137	18	component	component	NN
cord-277909-rn1dow26	137	19	:	:	:
cord-277909-rn1dow26	138	1	supplier	supplier	NN
cord-277909-rn1dow26	138	2	A	a	DT
cord-277909-rn1dow26	138	3	HPLC	HPLC	NNP
cord-277909-rn1dow26	138	4	positive	positive	JJ
cord-277909-rn1dow26	138	5	control	control	NN
cord-277909-rn1dow26	138	6	(	(	-LRB-
cord-277909-rn1dow26	138	7	−7	−7	NNP
cord-277909-rn1dow26	138	8	)	)	-RRB-
cord-277909-rn1dow26	138	9	,	,	,
cord-277909-rn1dow26	138	10	mean	mean	VBP
cord-277909-rn1dow26	138	11	C	C	NNP
cord-277909-rn1dow26	138	12	T	t	NN
cord-277909-rn1dow26	138	13	value	value	NN
cord-277909-rn1dow26	138	14	:	:	:
cord-277909-rn1dow26	138	15	30.68	30.68	CD
cord-277909-rn1dow26	138	16	;	;	:
cord-277909-rn1dow26	138	17	label	label	NNP
cord-277909-rn1dow26	138	18	5	5	CD
cord-277909-rn1dow26	138	19	,	,	,
cord-277909-rn1dow26	138	20	reaction	reaction	NN
cord-277909-rn1dow26	138	21	component	component	NN
cord-277909-rn1dow26	138	22	:	:	:
cord-277909-rn1dow26	138	23	supplier	supplier	NNP
cord-277909-rn1dow26	138	24	B	B	NNP
cord-277909-rn1dow26	138	25	negative	negative	JJ
cord-277909-rn1dow26	138	26	control	control	NN
cord-277909-rn1dow26	138	27	,	,	,
cord-277909-rn1dow26	138	28	mean	mean	VB
cord-277909-rn1dow26	138	29	C	C	NNP
cord-277909-rn1dow26	138	30	T	t	NN
cord-277909-rn1dow26	138	31	value	value	NN
cord-277909-rn1dow26	138	32	:	:	:
cord-277909-rn1dow26	138	33	40.00	40.00	CD
cord-277909-rn1dow26	138	34	;	;	:
cord-277909-rn1dow26	138	35	label	label	NNP
cord-277909-rn1dow26	138	36	6	6	CD
cord-277909-rn1dow26	138	37	,	,	,
cord-277909-rn1dow26	138	38	reaction	reaction	NN
cord-277909-rn1dow26	138	39	component	component	NN
cord-277909-rn1dow26	138	40	:	:	:
cord-277909-rn1dow26	138	41	supplier	supplier	NN
cord-277909-rn1dow26	138	42	B	B	NNP
cord-277909-rn1dow26	138	43	positive	positive	JJ
cord-277909-rn1dow26	138	44	control	control	NN
cord-277909-rn1dow26	138	45	(	(	-LRB-
cord-277909-rn1dow26	138	46	−7	−7	NNP
cord-277909-rn1dow26	138	47	)	)	-RRB-
cord-277909-rn1dow26	138	48	,	,	.
cord-277909-rn1dow26	139	1	mean	mean	VB
cord-277909-rn1dow26	140	1	C	C	NNP
cord-277909-rn1dow26	140	2	T	T	NNP
cord-277909-rn1dow26	140	3	value	value	NN
cord-277909-rn1dow26	140	4	:	:	:
cord-277909-rn1dow26	140	5	29.93	29.93	CD
cord-277909-rn1dow26	140	6	.	.	.
cord-277909-rn1dow26	141	1	A	a	DT
cord-277909-rn1dow26	141	2	full	full	JJ
cord-277909-rn1dow26	141	3	length	length	NN
cord-277909-rn1dow26	141	4	DNA	dna	NN
cord-277909-rn1dow26	141	5	oligonucleotide	oligonucleotide	NN
cord-277909-rn1dow26	141	6	representing	represent	VBG
cord-277909-rn1dow26	141	7	the	the	DT
cord-277909-rn1dow26	141	8	amplicon	amplicon	NN
cord-277909-rn1dow26	141	9	of	of	IN
cord-277909-rn1dow26	141	10	a	a	DT
cord-277909-rn1dow26	141	11	B19	B19	NNP
cord-277909-rn1dow26	141	12	real	real	JJ
cord-277909-rn1dow26	141	13	time	time	NN
cord-277909-rn1dow26	141	14	PCR	pcr	NN
cord-277909-rn1dow26	141	15	assay	assay	NN
cord-277909-rn1dow26	141	16	was	be	VBD
cord-277909-rn1dow26	141	17	synthesised	synthesise	VBN
cord-277909-rn1dow26	141	18	by	by	IN
cord-277909-rn1dow26	141	19	supplier	supplier	NN
cord-277909-rn1dow26	141	20	A.	a.	NN
cord-277909-rn1dow26	142	1	During	during	IN
cord-277909-rn1dow26	142	2	a	a	DT
cord-277909-rn1dow26	142	3	later	later	JJ
cord-277909-rn1dow26	142	4	investigation	investigation	NN
cord-277909-rn1dow26	142	5	into	into	IN
cord-277909-rn1dow26	142	6	assay	assay	NN
cord-277909-rn1dow26	142	7	contamination	contamination	NN
cord-277909-rn1dow26	142	8	following	follow	VBG
cord-277909-rn1dow26	142	9	a	a	DT
cord-277909-rn1dow26	142	10	reagent	reagent	NN
cord-277909-rn1dow26	142	11	change	change	NN
cord-277909-rn1dow26	142	12	,	,	,
cord-277909-rn1dow26	142	13	primers	primer	NNS
cord-277909-rn1dow26	142	14	and	and	CC
cord-277909-rn1dow26	142	15	probes	probe	NNS
cord-277909-rn1dow26	142	16	were	be	VBD
cord-277909-rn1dow26	142	17	again	again	RB
cord-277909-rn1dow26	142	18	purchased	purchase	VBN
cord-277909-rn1dow26	142	19	from	from	IN
cord-277909-rn1dow26	142	20	supplier	supplier	NN
cord-277909-rn1dow26	142	21	A	A	NNP
cord-277909-rn1dow26	142	22	(	(	-LRB-
cord-277909-rn1dow26	142	23	salt	salt	NN
cord-277909-rn1dow26	142	24	free	free	JJ
cord-277909-rn1dow26	142	25	and	and	CC
cord-277909-rn1dow26	142	26	HPLC	HPLC	NNP
cord-277909-rn1dow26	142	27	purified	purify	VBN
cord-277909-rn1dow26	142	28	)	)	-RRB-
cord-277909-rn1dow26	142	29	,	,	,
cord-277909-rn1dow26	142	30	and	and	CC
cord-277909-rn1dow26	142	31	from	from	IN
cord-277909-rn1dow26	142	32	an	an	DT
cord-277909-rn1dow26	142	33	alternative	alternative	JJ
cord-277909-rn1dow26	142	34	supplier	supplier	NN
cord-277909-rn1dow26	143	1	B.	B.	NNP
cord-277909-rn1dow26	143	2	The	the	DT
cord-277909-rn1dow26	143	3	reagents	reagent	NNS
cord-277909-rn1dow26	143	4	purchased	purchase	VBN
cord-277909-rn1dow26	143	5	from	from	IN
cord-277909-rn1dow26	143	6	supplier	supplier	NN
cord-277909-rn1dow26	143	7	B	B	NNP
cord-277909-rn1dow26	143	8	(	(	-LRB-
cord-277909-rn1dow26	143	9	5	5	CD
cord-277909-rn1dow26	143	10	and	and	CC
cord-277909-rn1dow26	143	11	6	6	CD
cord-277909-rn1dow26	143	12	)	)	-RRB-
cord-277909-rn1dow26	143	13	were	be	VBD
cord-277909-rn1dow26	143	14	clean	clean	JJ
cord-277909-rn1dow26	143	15	,	,	,
cord-277909-rn1dow26	143	16	whilst	whilst	IN
cord-277909-rn1dow26	143	17	those	those	DT
cord-277909-rn1dow26	143	18	from	from	IN
cord-277909-rn1dow26	143	19	supplier	supplier	NN
cord-277909-rn1dow26	143	20	A	A	NNP
cord-277909-rn1dow26	143	21	(	(	-LRB-
cord-277909-rn1dow26	143	22	1	1	CD
cord-277909-rn1dow26	143	23	+	+	SYM
cord-277909-rn1dow26	143	24	2	2	CD
cord-277909-rn1dow26	143	25	)	)	-RRB-
cord-277909-rn1dow26	143	26	were	be	VBD
cord-277909-rn1dow26	143	27	contaminated	contaminate	VBN
cord-277909-rn1dow26	143	28	with	with	IN
cord-277909-rn1dow26	143	29	the	the	DT
cord-277909-rn1dow26	143	30	previously	previously	RB
cord-277909-rn1dow26	143	31	synthesised	synthesise	VBN
cord-277909-rn1dow26	143	32	positive	positive	JJ
cord-277909-rn1dow26	143	33	control	control	NN
cord-277909-rn1dow26	143	34	,	,	,
cord-277909-rn1dow26	143	35	even	even	RB
cord-277909-rn1dow26	143	36	after	after	IN
cord-277909-rn1dow26	143	37	HPLC	HPLC	NNP
cord-277909-rn1dow26	143	38	purification	purification	NN
cord-277909-rn1dow26	143	39	(	(	-LRB-
cord-277909-rn1dow26	143	40	3	3	CD
cord-277909-rn1dow26	143	41	+	+	SYM
cord-277909-rn1dow26	143	42	4	4	CD
cord-277909-rn1dow26	143	43	)	)	-RRB-
cord-277909-rn1dow26	143	44	.	.	.
cord-277909-rn1dow26	144	1	specificity	specificity	NN
cord-277909-rn1dow26	144	2	.	.	.
cord-277909-rn1dow26	145	1	Serial	serial	JJ
cord-277909-rn1dow26	145	2	dilution	dilution	NN
cord-277909-rn1dow26	145	3	series	series	NN
cord-277909-rn1dow26	145	4	of	of	IN
cord-277909-rn1dow26	145	5	known	know	VBN
cord-277909-rn1dow26	145	6	positive	positive	JJ
cord-277909-rn1dow26	145	7	samples	sample	NNS
cord-277909-rn1dow26	145	8	may	may	MD
cord-277909-rn1dow26	145	9	also	also	RB
cord-277909-rn1dow26	145	10	be	be	VB
cord-277909-rn1dow26	145	11	prepared	prepare	VBN
cord-277909-rn1dow26	145	12	,	,	,
cord-277909-rn1dow26	145	13	and	and	CC
cord-277909-rn1dow26	145	14	tested	test	VBN
cord-277909-rn1dow26	145	15	in	in	IN
cord-277909-rn1dow26	145	16	parallel	parallel	NN
cord-277909-rn1dow26	145	17	in	in	IN
cord-277909-rn1dow26	145	18	the	the	DT
cord-277909-rn1dow26	145	19	new	new	JJ
cord-277909-rn1dow26	145	20	and	and	CC
cord-277909-rn1dow26	145	21	previous	previous	JJ
cord-277909-rn1dow26	145	22	assay	assay	NN
cord-277909-rn1dow26	145	23	systems	system	NNS
cord-277909-rn1dow26	145	24	to	to	TO
cord-277909-rn1dow26	145	25	determine	determine	VB
cord-277909-rn1dow26	145	26	which	which	WDT
cord-277909-rn1dow26	145	27	assay	assay	NN
cord-277909-rn1dow26	145	28	is	be	VBZ
cord-277909-rn1dow26	145	29	more	more	RBR
cord-277909-rn1dow26	145	30	sensitive	sensitive	JJ
cord-277909-rn1dow26	145	31	.	.	.
cord-277909-rn1dow26	146	1	Ideally	ideally	RB
cord-277909-rn1dow26	146	2	these	these	DT
cord-277909-rn1dow26	146	3	dilution	dilution	NN
cord-277909-rn1dow26	146	4	panels	panel	NNS
cord-277909-rn1dow26	146	5	should	should	MD
cord-277909-rn1dow26	146	6	represent	represent	VB
cord-277909-rn1dow26	146	7	all	all	DT
cord-277909-rn1dow26	146	8	subgroups	subgroup	NNS
cord-277909-rn1dow26	146	9	of	of	IN
cord-277909-rn1dow26	146	10	the	the	DT
cord-277909-rn1dow26	146	11	target	target	NN
cord-277909-rn1dow26	146	12	virus	virus	NN
cord-277909-rn1dow26	146	13	to	to	TO
cord-277909-rn1dow26	146	14	ensure	ensure	VB
cord-277909-rn1dow26	146	15	the	the	DT
cord-277909-rn1dow26	146	16	test	test	NN
cord-277909-rn1dow26	146	17	is	be	VBZ
cord-277909-rn1dow26	146	18	sensitive	sensitive	JJ
cord-277909-rn1dow26	146	19	for	for	IN
cord-277909-rn1dow26	146	20	all	all	DT
cord-277909-rn1dow26	146	21	types	type	NNS
cord-277909-rn1dow26	146	22	.	.	.
cord-277909-rn1dow26	147	1	A	a	DT
cord-277909-rn1dow26	147	2	new	new	JJ
cord-277909-rn1dow26	147	3	test	test	NN
cord-277909-rn1dow26	147	4	must	must	MD
cord-277909-rn1dow26	147	5	be	be	VB
cord-277909-rn1dow26	147	6	at	at	RB
cord-277909-rn1dow26	147	7	least	least	JJS
cord-277909-rn1dow26	147	8	as	as	RB
cord-277909-rn1dow26	147	9	sensitive	sensitive	JJ
cord-277909-rn1dow26	147	10	as	as	IN
cord-277909-rn1dow26	147	11	the	the	DT
cord-277909-rn1dow26	147	12	assay	assay	NN
cord-277909-rn1dow26	147	13	in	in	IN
cord-277909-rn1dow26	147	14	current	current	JJ
cord-277909-rn1dow26	147	15	use	use	NN
cord-277909-rn1dow26	147	16	,	,	,
cord-277909-rn1dow26	147	17	and	and	CC
cord-277909-rn1dow26	147	18	should	should	MD
cord-277909-rn1dow26	147	19	ideally	ideally	RB
cord-277909-rn1dow26	147	20	be	be	VB
cord-277909-rn1dow26	147	21	able	able	JJ
cord-277909-rn1dow26	147	22	to	to	TO
cord-277909-rn1dow26	147	23	detect	detect	VB
cord-277909-rn1dow26	147	24	a	a	DT
cord-277909-rn1dow26	147	25	wider	wide	JJR
cord-277909-rn1dow26	147	26	range	range	NN
cord-277909-rn1dow26	147	27	of	of	IN
cord-277909-rn1dow26	147	28	virus	virus	NN
cord-277909-rn1dow26	147	29	subtypes	subtype	NNS
cord-277909-rn1dow26	147	30	/	/	SYM
cord-277909-rn1dow26	147	31	variants	variant	NNS
cord-277909-rn1dow26	147	32	.	.	.
cord-277909-rn1dow26	148	1	An	an	DT
cord-277909-rn1dow26	148	2	additional	additional	JJ
cord-277909-rn1dow26	148	3	method	method	NN
cord-277909-rn1dow26	148	4	to	to	TO
cord-277909-rn1dow26	148	5	validate	validate	VB
cord-277909-rn1dow26	148	6	a	a	DT
cord-277909-rn1dow26	148	7	new	new	JJ
cord-277909-rn1dow26	148	8	assay	assay	NN
cord-277909-rn1dow26	148	9	is	be	VBZ
cord-277909-rn1dow26	148	10	to	to	TO
cord-277909-rn1dow26	148	11	test	test	VB
cord-277909-rn1dow26	148	12	the	the	DT
cord-277909-rn1dow26	148	13	assay	assay	NN
cord-277909-rn1dow26	148	14	using	use	VBG
cord-277909-rn1dow26	148	15	samples	sample	NNS
cord-277909-rn1dow26	148	16	from	from	IN
cord-277909-rn1dow26	148	17	an	an	DT
cord-277909-rn1dow26	148	18	external	external	JJ
cord-277909-rn1dow26	148	19	quality	quality	NN
cord-277909-rn1dow26	148	20	assurance	assurance	NN
cord-277909-rn1dow26	148	21	program	program	NN
cord-277909-rn1dow26	148	22	.	.	.
cord-277909-rn1dow26	149	1	Panels	panel	NNS
cord-277909-rn1dow26	149	2	may	may	MD
cord-277909-rn1dow26	149	3	be	be	VB
cord-277909-rn1dow26	149	4	obtained	obtain	VBN
cord-277909-rn1dow26	149	5	from	from	IN
cord-277909-rn1dow26	149	6	various	various	JJ
cord-277909-rn1dow26	149	7	sources	source	NNS
cord-277909-rn1dow26	149	8	,	,	,
cord-277909-rn1dow26	149	9	including	include	VBG
cord-277909-rn1dow26	149	10	National	National	NNP
cord-277909-rn1dow26	149	11	External	External	NNP
cord-277909-rn1dow26	149	12	Quality	Quality	NNP
cord-277909-rn1dow26	149	13	Assessment	Assessment	NNP
cord-277909-rn1dow26	149	14	Service	Service	NNP
cord-277909-rn1dow26	149	15	(	(	-LRB-
cord-277909-rn1dow26	149	16	NEQAS	NEQAS	NNP
cord-277909-rn1dow26	149	17	)	)	-RRB-
cord-277909-rn1dow26	149	18	and	and	CC
cord-277909-rn1dow26	149	19	Quality	Quality	NNP
cord-277909-rn1dow26	149	20	Control	Control	NNP
cord-277909-rn1dow26	149	21	for	for	IN
cord-277909-rn1dow26	149	22	Molecular	Molecular	NNP
cord-277909-rn1dow26	149	23	Diagnostics	Diagnostics	NNP
cord-277909-rn1dow26	149	24	(	(	-LRB-
cord-277909-rn1dow26	149	25	QCMD	QCMD	NNP
cord-277909-rn1dow26	149	26	)	)	-RRB-
cord-277909-rn1dow26	149	27	,	,	,
cord-277909-rn1dow26	149	28	and	and	CC
cord-277909-rn1dow26	149	29	the	the	DT
cord-277909-rn1dow26	149	30	expected	expect	VBN
cord-277909-rn1dow26	149	31	results	result	NNS
cord-277909-rn1dow26	149	32	may	may	MD
cord-277909-rn1dow26	149	33	be	be	VB
cord-277909-rn1dow26	149	34	compared	compare	VBN
cord-277909-rn1dow26	149	35	with	with	IN
cord-277909-rn1dow26	149	36	those	those	DT
cord-277909-rn1dow26	149	37	obtained	obtain	VBN
cord-277909-rn1dow26	149	38	from	from	IN
cord-277909-rn1dow26	149	39	the	the	DT
cord-277909-rn1dow26	149	40	old	old	JJ
cord-277909-rn1dow26	149	41	and	and	CC
cord-277909-rn1dow26	149	42	new	new	JJ
cord-277909-rn1dow26	149	43	assays	assay	NNS
cord-277909-rn1dow26	149	44	in	in	IN
cord-277909-rn1dow26	149	45	parallel	parallel	NN
cord-277909-rn1dow26	149	46	.	.	.
cord-277909-rn1dow26	150	1	The	the	DT
cord-277909-rn1dow26	150	2	use	use	NN
cord-277909-rn1dow26	150	3	of	of	IN
cord-277909-rn1dow26	150	4	such	such	JJ
cord-277909-rn1dow26	150	5	panels	panel	NNS
cord-277909-rn1dow26	150	6	also	also	RB
cord-277909-rn1dow26	150	7	allows	allow	VBZ
cord-277909-rn1dow26	150	8	the	the	DT
cord-277909-rn1dow26	150	9	comparison	comparison	NN
cord-277909-rn1dow26	150	10	of	of	IN
cord-277909-rn1dow26	150	11	assays	assay	NNS
cord-277909-rn1dow26	150	12	currently	currently	RB
cord-277909-rn1dow26	150	13	in	in	IN
cord-277909-rn1dow26	150	14	use	use	NN
cord-277909-rn1dow26	150	15	by	by	IN
cord-277909-rn1dow26	150	16	different	different	JJ
cord-277909-rn1dow26	150	17	laboratories	laboratory	NNS
cord-277909-rn1dow26	150	18	.	.	.
cord-277909-rn1dow26	151	1	When	when	WRB
cord-277909-rn1dow26	151	2	implementing	implement	VBG
cord-277909-rn1dow26	151	3	a	a	DT
cord-277909-rn1dow26	151	4	newly	newly	RB
cord-277909-rn1dow26	151	5	designed	design	VBN
cord-277909-rn1dow26	151	6	or	or	CC
cord-277909-rn1dow26	151	7	previously	previously	RB
cord-277909-rn1dow26	151	8	published	publish	VBN
cord-277909-rn1dow26	151	9	assay	assay	NN
cord-277909-rn1dow26	151	10	a	a	DT
cord-277909-rn1dow26	151	11	number	number	NN
cord-277909-rn1dow26	151	12	of	of	IN
cord-277909-rn1dow26	151	13	changes	change	NNS
cord-277909-rn1dow26	151	14	can	can	MD
cord-277909-rn1dow26	151	15	be	be	VB
cord-277909-rn1dow26	151	16	made	make	VBN
cord-277909-rn1dow26	151	17	in	in	IN
cord-277909-rn1dow26	151	18	order	order	NN
cord-277909-rn1dow26	151	19	to	to	TO
cord-277909-rn1dow26	151	20	reduce	reduce	VB
cord-277909-rn1dow26	151	21	the	the	DT
cord-277909-rn1dow26	151	22	turn	turn	NN
cord-277909-rn1dow26	151	23	around	around	IN
cord-277909-rn1dow26	151	24	time	time	NN
cord-277909-rn1dow26	151	25	of	of	IN
cord-277909-rn1dow26	151	26	the	the	DT
cord-277909-rn1dow26	151	27	assay	assay	NN
cord-277909-rn1dow26	151	28	and	and	CC
cord-277909-rn1dow26	151	29	increase	increase	VB
cord-277909-rn1dow26	151	30	laboratory	laboratory	NN
cord-277909-rn1dow26	151	31	throughput	throughput	NN
cord-277909-rn1dow26	151	32	.	.	.
cord-277909-rn1dow26	152	1	Multiplex	multiplex	JJ
cord-277909-rn1dow26	152	2	real	real	JJ
cord-277909-rn1dow26	152	3	time	time	NN
cord-277909-rn1dow26	152	4	PCR	pcr	NN
cord-277909-rn1dow26	152	5	assays	assay	NNS
cord-277909-rn1dow26	152	6	allow	allow	VBP
cord-277909-rn1dow26	152	7	the	the	DT
cord-277909-rn1dow26	152	8	detection	detection	NN
cord-277909-rn1dow26	152	9	of	of	IN
cord-277909-rn1dow26	152	10	multiple	multiple	JJ
cord-277909-rn1dow26	152	11	pathogens	pathogen	NNS
cord-277909-rn1dow26	152	12	within	within	IN
cord-277909-rn1dow26	152	13	a	a	DT
cord-277909-rn1dow26	152	14	single	single	JJ
cord-277909-rn1dow26	152	15	tube	tube	NN
cord-277909-rn1dow26	152	16	.	.	.
cord-277909-rn1dow26	153	1	The	the	DT
cord-277909-rn1dow26	153	2	utilisation	utilisation	NN
cord-277909-rn1dow26	153	3	of	of	IN
cord-277909-rn1dow26	153	4	such	such	JJ
cord-277909-rn1dow26	153	5	assays	assay	NNS
cord-277909-rn1dow26	153	6	reduces	reduce	VBZ
cord-277909-rn1dow26	153	7	overall	overall	JJ
cord-277909-rn1dow26	153	8	testing	testing	NN
cord-277909-rn1dow26	153	9	costs	cost	NNS
cord-277909-rn1dow26	153	10	and	and	CC
cord-277909-rn1dow26	153	11	turn	turn	VB
cord-277909-rn1dow26	153	12	around	around	RP
cord-277909-rn1dow26	153	13	times	time	NNS
cord-277909-rn1dow26	153	14	,	,	,
cord-277909-rn1dow26	153	15	enabling	enable	VBG
cord-277909-rn1dow26	153	16	a	a	DT
cord-277909-rn1dow26	153	17	high	high	JJ
cord-277909-rn1dow26	153	18	throughput	throughput	NN
cord-277909-rn1dow26	153	19	.	.	.
cord-277909-rn1dow26	154	1	There	there	EX
cord-277909-rn1dow26	154	2	are	be	VBP
cord-277909-rn1dow26	154	3	a	a	DT
cord-277909-rn1dow26	154	4	number	number	NN
cord-277909-rn1dow26	154	5	of	of	IN
cord-277909-rn1dow26	154	6	multiplex	multiplex	JJ
cord-277909-rn1dow26	154	7	real	real	JJ
cord-277909-rn1dow26	154	8	time	time	NN
cord-277909-rn1dow26	154	9	PCR	PCR	NNP
cord-277909-rn1dow26	154	10	assays	assay	NNS
cord-277909-rn1dow26	154	11	described	describe	VBN
cord-277909-rn1dow26	154	12	in	in	IN
cord-277909-rn1dow26	154	13	the	the	DT
cord-277909-rn1dow26	154	14	literature	literature	NN
cord-277909-rn1dow26	154	15	(	(	-LRB-
cord-277909-rn1dow26	154	16	Draganov	Draganov	NNP
cord-277909-rn1dow26	154	17	and	and	CC
cord-277909-rn1dow26	154	18	Kulvachev	Kulvachev	NNP
cord-277909-rn1dow26	154	19	,	,	,
cord-277909-rn1dow26	154	20	2004	2004	CD
cord-277909-rn1dow26	154	21	;	;	:
cord-277909-rn1dow26	155	1	Gunson	Gunson	NNP
cord-277909-rn1dow26	155	2	et	et	FW
cord-277909-rn1dow26	155	3	al	al	NNP
cord-277909-rn1dow26	155	4	.	.	.
cord-277909-rn1dow26	156	1	,	,	,
cord-277909-rn1dow26	156	2	2005	2005	CD
cord-277909-rn1dow26	156	3	;	;	:
cord-277909-rn1dow26	156	4	Hindiyeh	Hindiyeh	NNP
cord-277909-rn1dow26	156	5	et	et	NNP
cord-277909-rn1dow26	156	6	al	al	NNP
cord-277909-rn1dow26	156	7	.	.	NNP
cord-277909-rn1dow26	156	8	,	,	,
cord-277909-rn1dow26	156	9	2001	2001	CD
cord-277909-rn1dow26	156	10	;	;	:
cord-277909-rn1dow26	156	11	Richards	Richards	NNP
cord-277909-rn1dow26	156	12	et	et	NNP
cord-277909-rn1dow26	156	13	al	al	NNP
cord-277909-rn1dow26	156	14	.	.	NNP
cord-277909-rn1dow26	156	15	,	,	,
cord-277909-rn1dow26	156	16	2004	2004	CD
cord-277909-rn1dow26	156	17	;	;	:
cord-277909-rn1dow26	156	18	Templeton	Templeton	NNP
cord-277909-rn1dow26	156	19	et	et	NNP
cord-277909-rn1dow26	156	20	al	al	NNP
cord-277909-rn1dow26	156	21	.	.	NNP
cord-277909-rn1dow26	156	22	,	,	,
cord-277909-rn1dow26	156	23	2004	2004	CD
cord-277909-rn1dow26	156	24	)	)	-RRB-
cord-277909-rn1dow26	156	25	.	.	.
cord-277909-rn1dow26	157	1	We	-PRON-	PRP
cord-277909-rn1dow26	157	2	recently	recently	RB
cord-277909-rn1dow26	157	3	described	describe	VBD
cord-277909-rn1dow26	157	4	4	4	CD
cord-277909-rn1dow26	157	5	triplex	triplex	NN
cord-277909-rn1dow26	157	6	assays	assay	NNS
cord-277909-rn1dow26	157	7	designed	design	VBN
cord-277909-rn1dow26	157	8	to	to	TO
cord-277909-rn1dow26	157	9	detect	detect	VB
cord-277909-rn1dow26	157	10	12	12	CD
cord-277909-rn1dow26	157	11	respiratory	respiratory	JJ
cord-277909-rn1dow26	157	12	viral	viral	JJ
cord-277909-rn1dow26	157	13	pathogens	pathogen	NNS
cord-277909-rn1dow26	157	14	.	.	.
cord-277909-rn1dow26	158	1	Designing	design	VBG
cord-277909-rn1dow26	158	2	a	a	DT
cord-277909-rn1dow26	158	3	multiplex	multiplex	JJ
cord-277909-rn1dow26	158	4	real	real	JJ
cord-277909-rn1dow26	158	5	time	time	NN
cord-277909-rn1dow26	158	6	PCR	PCR	NNP
cord-277909-rn1dow26	158	7	is	be	VBZ
cord-277909-rn1dow26	158	8	a	a	DT
cord-277909-rn1dow26	158	9	complicated	complicated	JJ
cord-277909-rn1dow26	158	10	process	process	NN
cord-277909-rn1dow26	158	11	often	often	RB
cord-277909-rn1dow26	158	12	requiring	require	VBG
cord-277909-rn1dow26	158	13	a	a	DT
cord-277909-rn1dow26	158	14	great	great	JJ
cord-277909-rn1dow26	158	15	deal	deal	NN
cord-277909-rn1dow26	158	16	of	of	IN
cord-277909-rn1dow26	158	17	trial	trial	NN
cord-277909-rn1dow26	158	18	and	and	CC
cord-277909-rn1dow26	158	19	error	error	NN
cord-277909-rn1dow26	158	20	.	.	.
cord-277909-rn1dow26	159	1	Below	below	RB
cord-277909-rn1dow26	159	2	are	be	VBP
cord-277909-rn1dow26	159	3	outlined	outline	VBN
cord-277909-rn1dow26	159	4	some	some	DT
cord-277909-rn1dow26	159	5	general	general	JJ
cord-277909-rn1dow26	159	6	criteria	criterion	NNS
cord-277909-rn1dow26	159	7	that	that	WDT
cord-277909-rn1dow26	159	8	may	may	MD
cord-277909-rn1dow26	159	9	prove	prove	VB
cord-277909-rn1dow26	159	10	useful	useful	JJ
cord-277909-rn1dow26	159	11	when	when	WRB
cord-277909-rn1dow26	159	12	designing	design	VBG
cord-277909-rn1dow26	159	13	such	such	JJ
cord-277909-rn1dow26	159	14	assays	assay	NNS
cord-277909-rn1dow26	159	15	.	.	.
cord-277909-rn1dow26	160	1	In	in	IN
cord-277909-rn1dow26	160	2	order	order	NN
cord-277909-rn1dow26	160	3	to	to	TO
cord-277909-rn1dow26	160	4	design	design	VB
cord-277909-rn1dow26	160	5	appropriate	appropriate	JJ
cord-277909-rn1dow26	160	6	primers	primer	NNS
cord-277909-rn1dow26	160	7	and	and	CC
cord-277909-rn1dow26	160	8	probes	probe	NNS
cord-277909-rn1dow26	160	9	users	user	NNS
cord-277909-rn1dow26	160	10	should	should	MD
cord-277909-rn1dow26	160	11	follow	follow	VB
cord-277909-rn1dow26	160	12	the	the	DT
cord-277909-rn1dow26	160	13	development	development	NN
cord-277909-rn1dow26	160	14	protocols	protocol	NNS
cord-277909-rn1dow26	160	15	outlined	outline	VBN
cord-277909-rn1dow26	160	16	above	above	RB
cord-277909-rn1dow26	160	17	.	.	.
cord-277909-rn1dow26	161	1	However	however	RB
cord-277909-rn1dow26	161	2	,	,	,
cord-277909-rn1dow26	161	3	care	care	NN
cord-277909-rn1dow26	161	4	should	should	MD
cord-277909-rn1dow26	161	5	be	be	VB
cord-277909-rn1dow26	161	6	taken	take	VBN
cord-277909-rn1dow26	161	7	to	to	TO
cord-277909-rn1dow26	161	8	ensure	ensure	VB
cord-277909-rn1dow26	161	9	that	that	IN
cord-277909-rn1dow26	161	10	there	there	EX
cord-277909-rn1dow26	161	11	is	be	VBZ
cord-277909-rn1dow26	161	12	no	no	DT
cord-277909-rn1dow26	161	13	primer	primer	NN
cord-277909-rn1dow26	161	14	or	or	CC
cord-277909-rn1dow26	161	15	probe	probe	NN
cord-277909-rn1dow26	161	16	interaction	interaction	NN
cord-277909-rn1dow26	161	17	that	that	WDT
cord-277909-rn1dow26	161	18	may	may	MD
cord-277909-rn1dow26	161	19	reduce	reduce	VB
cord-277909-rn1dow26	161	20	the	the	DT
cord-277909-rn1dow26	161	21	sensitivity	sensitivity	NN
cord-277909-rn1dow26	161	22	or	or	CC
cord-277909-rn1dow26	161	23	efficiency	efficiency	NN
cord-277909-rn1dow26	161	24	of	of	IN
cord-277909-rn1dow26	161	25	the	the	DT
cord-277909-rn1dow26	161	26	PCR	PCR	NNP
cord-277909-rn1dow26	161	27	reaction	reaction	NN
cord-277909-rn1dow26	161	28	.	.	.
cord-277909-rn1dow26	162	1	Most	Most	JJS
cord-277909-rn1dow26	162	2	primer	primer	NN
cord-277909-rn1dow26	162	3	design	design	NN
cord-277909-rn1dow26	162	4	software	software	NN
cord-277909-rn1dow26	162	5	will	will	MD
cord-277909-rn1dow26	162	6	allow	allow	VB
cord-277909-rn1dow26	162	7	primer	primer	NN
cord-277909-rn1dow26	162	8	-	-	HYPH
cord-277909-rn1dow26	162	9	probe	probe	NN
cord-277909-rn1dow26	162	10	interactions	interaction	NNS
cord-277909-rn1dow26	162	11	to	to	TO
cord-277909-rn1dow26	162	12	be	be	VB
cord-277909-rn1dow26	162	13	examined	examine	VBN
cord-277909-rn1dow26	162	14	.	.	.
cord-277909-rn1dow26	163	1	In	in	IN
cord-277909-rn1dow26	163	2	order	order	NN
cord-277909-rn1dow26	163	3	to	to	TO
cord-277909-rn1dow26	163	4	optimise	optimise	VB
cord-277909-rn1dow26	163	5	the	the	DT
cord-277909-rn1dow26	163	6	multiplex	multiplex	NN
cord-277909-rn1dow26	163	7	assay	assay	NN
cord-277909-rn1dow26	163	8	each	each	DT
cord-277909-rn1dow26	163	9	separate	separate	JJ
cord-277909-rn1dow26	163	10	PCR	pcr	NN
cord-277909-rn1dow26	163	11	should	should	MD
cord-277909-rn1dow26	163	12	be	be	VB
cord-277909-rn1dow26	163	13	optimised	optimise	VBN
cord-277909-rn1dow26	163	14	separately	separately	RB
cord-277909-rn1dow26	163	15	before	before	IN
cord-277909-rn1dow26	163	16	being	be	VBG
cord-277909-rn1dow26	163	17	assessed	assess	VBN
cord-277909-rn1dow26	163	18	when	when	WRB
cord-277909-rn1dow26	163	19	added	add	VBN
cord-277909-rn1dow26	163	20	together	together	RB
cord-277909-rn1dow26	163	21	(	(	-LRB-
cord-277909-rn1dow26	163	22	see	see	VB
cord-277909-rn1dow26	163	23	above	above	IN
cord-277909-rn1dow26	163	24	section	section	NN
cord-277909-rn1dow26	163	25	for	for	IN
cord-277909-rn1dow26	163	26	details	detail	NNS
cord-277909-rn1dow26	163	27	)	)	-RRB-
cord-277909-rn1dow26	163	28	.	.	.
cord-277909-rn1dow26	164	1	Further	further	JJ
cord-277909-rn1dow26	164	2	experiments	experiment	NNS
cord-277909-rn1dow26	164	3	should	should	MD
cord-277909-rn1dow26	164	4	include	include	VB
cord-277909-rn1dow26	164	5	assessing	assess	VBG
cord-277909-rn1dow26	164	6	the	the	DT
cord-277909-rn1dow26	164	7	sensitivity	sensitivity	NN
cord-277909-rn1dow26	164	8	of	of	IN
cord-277909-rn1dow26	164	9	the	the	DT
cord-277909-rn1dow26	164	10	multiplex	multiplex	JJ
cord-277909-rn1dow26	164	11	assay	assay	NN
cord-277909-rn1dow26	164	12	for	for	IN
cord-277909-rn1dow26	164	13	the	the	DT
cord-277909-rn1dow26	164	14	simultaneous	simultaneous	JJ
cord-277909-rn1dow26	164	15	detection	detection	NN
cord-277909-rn1dow26	164	16	of	of	IN
cord-277909-rn1dow26	164	17	mixed	mixed	JJ
cord-277909-rn1dow26	164	18	infections	infection	NNS
cord-277909-rn1dow26	164	19	(	(	-LRB-
cord-277909-rn1dow26	164	20	real	real	JJ
cord-277909-rn1dow26	164	21	or	or	CC
cord-277909-rn1dow26	164	22	spiked	spiked	JJ
cord-277909-rn1dow26	164	23	)	)	-RRB-
cord-277909-rn1dow26	164	24	and	and	CC
cord-277909-rn1dow26	164	25	low	low	JJ
cord-277909-rn1dow26	164	26	copy	copy	NN
cord-277909-rn1dow26	164	27	targets	target	NNS
cord-277909-rn1dow26	164	28	in	in	IN
cord-277909-rn1dow26	164	29	high	high	JJ
cord-277909-rn1dow26	164	30	copy	copy	NN
cord-277909-rn1dow26	164	31	backgrounds	background	NNS
cord-277909-rn1dow26	164	32	.	.	.
cord-277909-rn1dow26	165	1	Ideally	ideally	RB
cord-277909-rn1dow26	165	2	,	,	,
cord-277909-rn1dow26	165	3	no	no	DT
cord-277909-rn1dow26	165	4	loss	loss	NN
cord-277909-rn1dow26	165	5	in	in	IN
cord-277909-rn1dow26	165	6	sensitivity	sensitivity	NN
cord-277909-rn1dow26	165	7	should	should	MD
cord-277909-rn1dow26	165	8	be	be	VB
cord-277909-rn1dow26	165	9	observed	observe	VBN
cord-277909-rn1dow26	165	10	when	when	WRB
cord-277909-rn1dow26	165	11	additional	additional	JJ
cord-277909-rn1dow26	165	12	primers	primer	NNS
cord-277909-rn1dow26	165	13	are	be	VBP
cord-277909-rn1dow26	165	14	added	add	VBN
cord-277909-rn1dow26	165	15	.	.	.
cord-277909-rn1dow26	166	1	However	however	RB
cord-277909-rn1dow26	166	2	,	,	,
cord-277909-rn1dow26	166	3	if	if	IN
cord-277909-rn1dow26	166	4	the	the	DT
cord-277909-rn1dow26	166	5	multiplex	multiplex	JJ
cord-277909-rn1dow26	166	6	assay	assay	NN
cord-277909-rn1dow26	166	7	is	be	VBZ
cord-277909-rn1dow26	166	8	less	less	RBR
cord-277909-rn1dow26	166	9	sensitive	sensitive	JJ
cord-277909-rn1dow26	166	10	,	,	,
cord-277909-rn1dow26	166	11	altering	alter	VBG
cord-277909-rn1dow26	166	12	the	the	DT
cord-277909-rn1dow26	166	13	ratio	ratio	NN
cord-277909-rn1dow26	166	14	of	of	IN
cord-277909-rn1dow26	166	15	primers	primer	NNS
cord-277909-rn1dow26	166	16	/	/	SYM
cord-277909-rn1dow26	166	17	probes	probe	NNS
cord-277909-rn1dow26	166	18	concentrations	concentration	NNS
cord-277909-rn1dow26	166	19	may	may	MD
cord-277909-rn1dow26	166	20	prove	prove	VB
cord-277909-rn1dow26	166	21	useful	useful	JJ
cord-277909-rn1dow26	166	22	.	.	.
cord-277909-rn1dow26	167	1	Alternatively	alternatively	RB
cord-277909-rn1dow26	167	2	,	,	,
cord-277909-rn1dow26	167	3	changing	change	VBG
cord-277909-rn1dow26	167	4	the	the	DT
cord-277909-rn1dow26	167	5	concentration	concentration	NN
cord-277909-rn1dow26	167	6	of	of	IN
cord-277909-rn1dow26	167	7	PCR	PCR	NNP
cord-277909-rn1dow26	167	8	reagents	reagent	NNS
cord-277909-rn1dow26	167	9	(	(	-LRB-
cord-277909-rn1dow26	167	10	enzyme	enzyme	NN
cord-277909-rn1dow26	167	11	,	,	,
cord-277909-rn1dow26	167	12	Mg	Mg	NNP
cord-277909-rn1dow26	167	13	2	2	CD
cord-277909-rn1dow26	167	14	+	+	SYM
cord-277909-rn1dow26	167	15	,	,	,
cord-277909-rn1dow26	167	16	dNTPs	dNTPs	NNP
cord-277909-rn1dow26	167	17	etc	etc	FW
cord-277909-rn1dow26	167	18	.	.	.
cord-277909-rn1dow26	167	19	)	)	-RRB-
cord-277909-rn1dow26	167	20	may	may	MD
cord-277909-rn1dow26	167	21	also	also	RB
cord-277909-rn1dow26	167	22	be	be	VB
cord-277909-rn1dow26	167	23	beneficial	beneficial	JJ
cord-277909-rn1dow26	167	24	.	.	.
cord-277909-rn1dow26	168	1	Some	some	DT
cord-277909-rn1dow26	168	2	manufacturers	manufacturer	NNS
cord-277909-rn1dow26	168	3	are	be	VBP
cord-277909-rn1dow26	168	4	now	now	RB
cord-277909-rn1dow26	168	5	producing	produce	VBG
cord-277909-rn1dow26	168	6	real	real	JJ
cord-277909-rn1dow26	168	7	time	time	NN
cord-277909-rn1dow26	168	8	reaction	reaction	NN
cord-277909-rn1dow26	168	9	mixes	mix	NNS
cord-277909-rn1dow26	168	10	specifically	specifically	RB
cord-277909-rn1dow26	168	11	designed	design	VBN
cord-277909-rn1dow26	168	12	for	for	IN
cord-277909-rn1dow26	168	13	use	use	NN
cord-277909-rn1dow26	168	14	with	with	IN
cord-277909-rn1dow26	168	15	multiplex	multiplex	JJ
cord-277909-rn1dow26	168	16	assays	assay	NNS
cord-277909-rn1dow26	168	17	,	,	,
cord-277909-rn1dow26	168	18	and	and	CC
cord-277909-rn1dow26	168	19	provide	provide	VB
cord-277909-rn1dow26	168	20	guidelines	guideline	NNS
cord-277909-rn1dow26	168	21	on	on	IN
cord-277909-rn1dow26	168	22	the	the	DT
cord-277909-rn1dow26	168	23	optimal	optimal	JJ
cord-277909-rn1dow26	168	24	primer	primer	NN
cord-277909-rn1dow26	168	25	and	and	CC
cord-277909-rn1dow26	168	26	probe	probe	VB
cord-277909-rn1dow26	168	27	concentrations	concentration	NNS
cord-277909-rn1dow26	168	28	to	to	TO
cord-277909-rn1dow26	168	29	use	use	VB
cord-277909-rn1dow26	168	30	.	.	.
cord-277909-rn1dow26	169	1	However	however	RB
cord-277909-rn1dow26	169	2	,	,	,
cord-277909-rn1dow26	169	3	if	if	IN
cord-277909-rn1dow26	169	4	all	all	PDT
cord-277909-rn1dow26	169	5	these	these	DT
cord-277909-rn1dow26	169	6	factors	factor	NNS
cord-277909-rn1dow26	169	7	fail	fail	VBP
cord-277909-rn1dow26	169	8	to	to	TO
cord-277909-rn1dow26	169	9	improve	improve	VB
cord-277909-rn1dow26	169	10	the	the	DT
cord-277909-rn1dow26	169	11	sensitivity	sensitivity	NN
cord-277909-rn1dow26	169	12	of	of	IN
cord-277909-rn1dow26	169	13	the	the	DT
cord-277909-rn1dow26	169	14	multiplex	multiplex	JJ
cord-277909-rn1dow26	169	15	assay	assay	NN
cord-277909-rn1dow26	169	16	then	then	RB
cord-277909-rn1dow26	169	17	some	some	DT
cord-277909-rn1dow26	169	18	or	or	CC
cord-277909-rn1dow26	169	19	all	all	DT
cord-277909-rn1dow26	169	20	of	of	IN
cord-277909-rn1dow26	169	21	the	the	DT
cord-277909-rn1dow26	169	22	primer	primer	NN
cord-277909-rn1dow26	169	23	and	and	CC
cord-277909-rn1dow26	169	24	probes	probe	NNS
cord-277909-rn1dow26	169	25	may	may	MD
cord-277909-rn1dow26	169	26	have	have	VB
cord-277909-rn1dow26	169	27	to	to	TO
cord-277909-rn1dow26	169	28	be	be	VB
cord-277909-rn1dow26	169	29	redesigned	redesign	VBN
cord-277909-rn1dow26	169	30	.	.	.
cord-277909-rn1dow26	170	1	The	the	DT
cord-277909-rn1dow26	170	2	number	number	NN
cord-277909-rn1dow26	170	3	of	of	IN
cord-277909-rn1dow26	170	4	targets	target	NNS
cord-277909-rn1dow26	170	5	detected	detect	VBN
cord-277909-rn1dow26	170	6	in	in	IN
cord-277909-rn1dow26	170	7	one	one	CD
cord-277909-rn1dow26	170	8	assay	assay	NN
cord-277909-rn1dow26	170	9	is	be	VBZ
cord-277909-rn1dow26	170	10	limited	limit	VBN
cord-277909-rn1dow26	170	11	by	by	IN
cord-277909-rn1dow26	170	12	the	the	DT
cord-277909-rn1dow26	170	13	number	number	NN
cord-277909-rn1dow26	170	14	of	of	IN
cord-277909-rn1dow26	170	15	detection	detection	NN
cord-277909-rn1dow26	170	16	channels	channel	NNS
cord-277909-rn1dow26	170	17	available	available	JJ
cord-277909-rn1dow26	170	18	on	on	IN
cord-277909-rn1dow26	170	19	the	the	DT
cord-277909-rn1dow26	170	20	real	real	JJ
cord-277909-rn1dow26	170	21	time	time	NN
cord-277909-rn1dow26	170	22	platform	platform	NN
cord-277909-rn1dow26	170	23	and	and	CC
cord-277909-rn1dow26	170	24	the	the	DT
cord-277909-rn1dow26	170	25	number	number	NN
cord-277909-rn1dow26	170	26	of	of	IN
cord-277909-rn1dow26	170	27	fluorescent	fluorescent	NN
cord-277909-rn1dow26	170	28	-	-	HYPH
cord-277909-rn1dow26	170	29	labelled	label	VBN
cord-277909-rn1dow26	170	30	dyes	dye	NNS
cord-277909-rn1dow26	170	31	available	available	JJ
cord-277909-rn1dow26	170	32	.	.	.
cord-277909-rn1dow26	171	1	Newer	newer	JJ
cord-277909-rn1dow26	171	2	machines	machine	NNS
cord-277909-rn1dow26	171	3	tend	tend	VBP
cord-277909-rn1dow26	171	4	to	to	TO
cord-277909-rn1dow26	171	5	have	have	VB
cord-277909-rn1dow26	171	6	five	five	CD
cord-277909-rn1dow26	171	7	channels	channel	NNS
cord-277909-rn1dow26	171	8	.	.	.
cord-277909-rn1dow26	172	1	Although	although	IN
cord-277909-rn1dow26	172	2	there	there	EX
cord-277909-rn1dow26	172	3	are	be	VBP
cord-277909-rn1dow26	172	4	many	many	JJ
cord-277909-rn1dow26	172	5	fluorescent	fluorescent	JJ
cord-277909-rn1dow26	172	6	dyes	dye	NNS
cord-277909-rn1dow26	172	7	available	available	JJ
cord-277909-rn1dow26	172	8	,	,	,
cord-277909-rn1dow26	172	9	many	many	JJ
cord-277909-rn1dow26	172	10	of	of	IN
cord-277909-rn1dow26	172	11	the	the	DT
cord-277909-rn1dow26	172	12	excitation	excitation	NN
cord-277909-rn1dow26	172	13	/	/	SYM
cord-277909-rn1dow26	172	14	emission	emission	NN
cord-277909-rn1dow26	172	15	spectra	spectrum	NNS
cord-277909-rn1dow26	172	16	overlap	overlap	VBP
cord-277909-rn1dow26	172	17	and	and	CC
cord-277909-rn1dow26	172	18	thus	thus	RB
cord-277909-rn1dow26	172	19	only	only	RB
cord-277909-rn1dow26	172	20	certain	certain	JJ
cord-277909-rn1dow26	172	21	combinations	combination	NNS
cord-277909-rn1dow26	172	22	can	can	MD
cord-277909-rn1dow26	172	23	be	be	VB
cord-277909-rn1dow26	172	24	used	use	VBN
cord-277909-rn1dow26	172	25	.	.	.
cord-277909-rn1dow26	173	1	At	at	IN
cord-277909-rn1dow26	173	2	present	present	JJ
cord-277909-rn1dow26	173	3	we	-PRON-	PRP
cord-277909-rn1dow26	173	4	are	be	VBP
cord-277909-rn1dow26	173	5	using	use	VBG
cord-277909-rn1dow26	173	6	FAM	FAM	NNP
cord-277909-rn1dow26	173	7	,	,	,
cord-277909-rn1dow26	173	8	VIC	VIC	NNP
cord-277909-rn1dow26	173	9	,	,	,
cord-277909-rn1dow26	173	10	and	and	CC
cord-277909-rn1dow26	173	11	Cy5	cy5	NN
cord-277909-rn1dow26	173	12	detectors	detector	NNS
cord-277909-rn1dow26	173	13	as	as	IN
cord-277909-rn1dow26	173	14	these	these	DT
cord-277909-rn1dow26	173	15	are	be	VBP
cord-277909-rn1dow26	173	16	optimal	optimal	JJ
cord-277909-rn1dow26	173	17	for	for	IN
cord-277909-rn1dow26	173	18	the	the	DT
cord-277909-rn1dow26	173	19	filter	filter	NN
cord-277909-rn1dow26	173	20	set	set	VBN
cord-277909-rn1dow26	173	21	utilised	utilise	VBN
cord-277909-rn1dow26	173	22	in	in	IN
cord-277909-rn1dow26	173	23	the	the	DT
cord-277909-rn1dow26	173	24	ABI	ABI	NNP
cord-277909-rn1dow26	173	25	7500	7500	CD
cord-277909-rn1dow26	174	1	(	(	-LRB-
cord-277909-rn1dow26	174	2	please	please	UH
cord-277909-rn1dow26	174	3	note	note	VB
cord-277909-rn1dow26	174	4	that	that	IN
cord-277909-rn1dow26	174	5	this	this	DT
cord-277909-rn1dow26	174	6	may	may	MD
cord-277909-rn1dow26	174	7	differ	differ	VB
cord-277909-rn1dow26	174	8	when	when	WRB
cord-277909-rn1dow26	174	9	using	use	VBG
cord-277909-rn1dow26	174	10	other	other	JJ
cord-277909-rn1dow26	174	11	platforms	platform	NNS
cord-277909-rn1dow26	174	12	)	)	-RRB-
cord-277909-rn1dow26	174	13	.	.	.
cord-277909-rn1dow26	175	1	Syndrome	syndrome	NN
cord-277909-rn1dow26	175	2	based	base	VBN
cord-277909-rn1dow26	175	3	testing	testing	NN
cord-277909-rn1dow26	175	4	policies	policy	NNS
cord-277909-rn1dow26	175	5	are	be	VBP
cord-277909-rn1dow26	175	6	ideal	ideal	JJ
cord-277909-rn1dow26	175	7	for	for	IN
cord-277909-rn1dow26	175	8	rapid	rapid	JJ
cord-277909-rn1dow26	175	9	,	,	,
cord-277909-rn1dow26	175	10	high	high	JJ
cord-277909-rn1dow26	175	11	throughput	throughput	NN
cord-277909-rn1dow26	175	12	testing	testing	NN
cord-277909-rn1dow26	175	13	.	.	.
cord-277909-rn1dow26	176	1	In	in	IN
cord-277909-rn1dow26	176	2	our	-PRON-	PRP$
cord-277909-rn1dow26	176	3	laboratory	laboratory	NN
cord-277909-rn1dow26	176	4	we	-PRON-	PRP
cord-277909-rn1dow26	176	5	offer	offer	VBP
cord-277909-rn1dow26	176	6	a	a	DT
cord-277909-rn1dow26	176	7	number	number	NN
cord-277909-rn1dow26	176	8	of	of	IN
cord-277909-rn1dow26	176	9	such	such	JJ
cord-277909-rn1dow26	176	10	"	"	``
cord-277909-rn1dow26	176	11	menus	menu	NNS
cord-277909-rn1dow26	176	12	"	"	''
cord-277909-rn1dow26	176	13	,	,	,
cord-277909-rn1dow26	176	14	which	which	WDT
cord-277909-rn1dow26	176	15	negate	negate	VBP
cord-277909-rn1dow26	176	16	the	the	DT
cord-277909-rn1dow26	176	17	need	need	NN
cord-277909-rn1dow26	176	18	for	for	IN
cord-277909-rn1dow26	176	19	clinical	clinical	JJ
cord-277909-rn1dow26	176	20	coding	coding	NN
cord-277909-rn1dow26	176	21	and	and	CC
cord-277909-rn1dow26	176	22	allow	allow	VB
cord-277909-rn1dow26	176	23	samples	sample	NNS
cord-277909-rn1dow26	176	24	to	to	TO
cord-277909-rn1dow26	176	25	be	be	VB
cord-277909-rn1dow26	176	26	tested	test	VBN
cord-277909-rn1dow26	176	27	immediately	immediately	RB
cord-277909-rn1dow26	176	28	upon	upon	IN
cord-277909-rn1dow26	176	29	receipt	receipt	NN
cord-277909-rn1dow26	176	30	(	(	-LRB-
cord-277909-rn1dow26	176	31	Table	Table	NNP
cord-277909-rn1dow26	176	32	3	3	CD
cord-277909-rn1dow26	176	33	)	)	-RRB-
cord-277909-rn1dow26	176	34	.	.	.
cord-277909-rn1dow26	177	1	For	for	IN
cord-277909-rn1dow26	177	2	example	example	NN
cord-277909-rn1dow26	177	3	,	,	,
cord-277909-rn1dow26	177	4	all	all	DT
cord-277909-rn1dow26	177	5	CSF	CSF	NNP
cord-277909-rn1dow26	177	6	samples	sample	NNS
cord-277909-rn1dow26	177	7	from	from	IN
cord-277909-rn1dow26	177	8	patients	patient	NNS
cord-277909-rn1dow26	177	9	with	with	IN
cord-277909-rn1dow26	177	10	neurological	neurological	JJ
cord-277909-rn1dow26	177	11	illnesses	illness	NNS
cord-277909-rn1dow26	177	12	such	such	JJ
cord-277909-rn1dow26	177	13	as	as	IN
cord-277909-rn1dow26	177	14	encephalitis	encephalitis	NN
cord-277909-rn1dow26	177	15	or	or	CC
cord-277909-rn1dow26	177	16	meningitis	meningitis	NN
cord-277909-rn1dow26	177	17	are	be	VBP
cord-277909-rn1dow26	177	18	tested	test	VBN
cord-277909-rn1dow26	177	19	for	for	IN
cord-277909-rn1dow26	177	20	enterovirus	enterovirus	NN
cord-277909-rn1dow26	177	21	,	,	,
cord-277909-rn1dow26	177	22	HSV	HSV	NNP
cord-277909-rn1dow26	177	23	(	(	-LRB-
cord-277909-rn1dow26	177	24	1	1	CD
cord-277909-rn1dow26	177	25	and	and	CC
cord-277909-rn1dow26	177	26	2	2	CD
cord-277909-rn1dow26	177	27	)	)	-RRB-
cord-277909-rn1dow26	177	28	,	,	,
cord-277909-rn1dow26	177	29	VZV	VZV	NNP
cord-277909-rn1dow26	177	30	,	,	,
cord-277909-rn1dow26	177	31	EBV	EBV	NNP
cord-277909-rn1dow26	177	32	,	,	,
cord-277909-rn1dow26	177	33	CMV	CMV	NNP
cord-277909-rn1dow26	177	34	and	and	CC
cord-277909-rn1dow26	177	35	HHV-6	HHV-6	NNP
cord-277909-rn1dow26	177	36	regardless	regardless	RB
cord-277909-rn1dow26	177	37	of	of	IN
cord-277909-rn1dow26	177	38	patient	patient	JJ
cord-277909-rn1dow26	177	39	or	or	CC
cord-277909-rn1dow26	177	40	clinical	clinical	JJ
cord-277909-rn1dow26	177	41	details	detail	NNS
cord-277909-rn1dow26	177	42	.	.	.
cord-277909-rn1dow26	178	1	Similar	similar	JJ
cord-277909-rn1dow26	178	2	testing	testing	NN
cord-277909-rn1dow26	178	3	protocols	protocol	NNS
cord-277909-rn1dow26	178	4	are	be	VBP
cord-277909-rn1dow26	178	5	in	in	IN
cord-277909-rn1dow26	178	6	place	place	NN
cord-277909-rn1dow26	178	7	for	for	IN
cord-277909-rn1dow26	178	8	urethritis	urethritis	NN
cord-277909-rn1dow26	178	9	,	,	,
cord-277909-rn1dow26	178	10	gastroenteritis	gastroenteritis	NN
cord-277909-rn1dow26	178	11	,	,	,
cord-277909-rn1dow26	178	12	respiratory	respiratory	JJ
cord-277909-rn1dow26	178	13	illness	illness	NN
cord-277909-rn1dow26	178	14	and	and	CC
cord-277909-rn1dow26	178	15	eye	eye	NN
cord-277909-rn1dow26	178	16	infections	infection	NNS
cord-277909-rn1dow26	178	17	.	.	.
cord-277909-rn1dow26	179	1	However	however	RB
cord-277909-rn1dow26	179	2	,	,	,
cord-277909-rn1dow26	179	3	although	although	IN
cord-277909-rn1dow26	179	4	such	such	JJ
cord-277909-rn1dow26	179	5	policies	policy	NNS
cord-277909-rn1dow26	179	6	aid	aid	VBP
cord-277909-rn1dow26	179	7	high	high	JJ
cord-277909-rn1dow26	179	8	throughput	throughput	NN
cord-277909-rn1dow26	179	9	and	and	CC
cord-277909-rn1dow26	179	10	reduce	reduce	VB
cord-277909-rn1dow26	179	11	turn	turn	NN
cord-277909-rn1dow26	179	12	around	around	RP
cord-277909-rn1dow26	179	13	times	time	NNS
cord-277909-rn1dow26	179	14	(	(	-LRB-
cord-277909-rn1dow26	179	15	sample	sample	NN
cord-277909-rn1dow26	179	16	receipt	receipt	NN
cord-277909-rn1dow26	179	17	until	until	IN
cord-277909-rn1dow26	179	18	when	when	WRB
cord-277909-rn1dow26	179	19	result	result	NN
cord-277909-rn1dow26	179	20	is	be	VBZ
cord-277909-rn1dow26	179	21	ready	ready	JJ
cord-277909-rn1dow26	179	22	)	)	-RRB-
cord-277909-rn1dow26	179	23	,	,	,
cord-277909-rn1dow26	179	24	it	-PRON-	PRP
cord-277909-rn1dow26	179	25	should	should	MD
cord-277909-rn1dow26	179	26	be	be	VB
cord-277909-rn1dow26	179	27	noted	note	VBN
cord-277909-rn1dow26	179	28	that	that	IN
cord-277909-rn1dow26	179	29	they	-PRON-	PRP
cord-277909-rn1dow26	179	30	may	may	MD
cord-277909-rn1dow26	179	31	be	be	VB
cord-277909-rn1dow26	179	32	more	more	RBR
cord-277909-rn1dow26	179	33	expensive	expensive	JJ
cord-277909-rn1dow26	179	34	and	and	CC
cord-277909-rn1dow26	179	35	will	will	MD
cord-277909-rn1dow26	179	36	occasionally	occasionally	RB
cord-277909-rn1dow26	179	37	produce	produce	VB
cord-277909-rn1dow26	179	38	results	result	NNS
cord-277909-rn1dow26	179	39	that	that	WDT
cord-277909-rn1dow26	179	40	are	be	VBP
cord-277909-rn1dow26	179	41	difficult	difficult	JJ
cord-277909-rn1dow26	179	42	to	to	TO
cord-277909-rn1dow26	179	43	interpret	interpret	VB
cord-277909-rn1dow26	179	44	,	,	,
cord-277909-rn1dow26	179	45	e.g.	e.g.	IN
cord-277909-rn1dow26	179	46	herpes	herpes	NN
cord-277909-rn1dow26	179	47	viruses	virus	NNS
cord-277909-rn1dow26	179	48	in	in	IN
cord-277909-rn1dow26	179	49	respiratory	respiratory	JJ
cord-277909-rn1dow26	179	50	samples	sample	NNS
cord-277909-rn1dow26	179	51	(	(	-LRB-
cord-277909-rn1dow26	179	52	see	see	VB
cord-277909-rn1dow26	179	53	below	below	RB
cord-277909-rn1dow26	179	54	)	)	-RRB-
cord-277909-rn1dow26	179	55	.	.	.
cord-277909-rn1dow26	179	56	 	 	_SP
cord-277909-rn1dow26	180	1	Automation	automation	NN
cord-277909-rn1dow26	180	2	of	of	IN
cord-277909-rn1dow26	180	3	the	the	DT
cord-277909-rn1dow26	180	4	extraction	extraction	NN
cord-277909-rn1dow26	180	5	and	and	CC
cord-277909-rn1dow26	180	6	liquid	liquid	NN
cord-277909-rn1dow26	180	7	handling	handling	NN
cord-277909-rn1dow26	180	8	process	process	NN
cord-277909-rn1dow26	180	9	has	have	VBZ
cord-277909-rn1dow26	180	10	led	lead	VBN
cord-277909-rn1dow26	180	11	to	to	IN
cord-277909-rn1dow26	180	12	significant	significant	JJ
cord-277909-rn1dow26	180	13	improvements	improvement	NNS
cord-277909-rn1dow26	180	14	in	in	IN
cord-277909-rn1dow26	180	15	turn	turn	NN
cord-277909-rn1dow26	180	16	around	around	RP
cord-277909-rn1dow26	180	17	times	time	NNS
cord-277909-rn1dow26	180	18	and	and	CC
cord-277909-rn1dow26	180	19	allows	allow	VBZ
cord-277909-rn1dow26	180	20	high	high	JJ
cord-277909-rn1dow26	180	21	throughput	throughput	NN
cord-277909-rn1dow26	180	22	with	with	IN
cord-277909-rn1dow26	180	23	a	a	DT
cord-277909-rn1dow26	180	24	reduced	reduce	VBN
cord-277909-rn1dow26	180	25	risk	risk	NN
cord-277909-rn1dow26	180	26	of	of	IN
cord-277909-rn1dow26	180	27	user	user	NN
cord-277909-rn1dow26	180	28	error	error	NN
cord-277909-rn1dow26	180	29	.	.	.
cord-277909-rn1dow26	181	1	Many	many	JJ
cord-277909-rn1dow26	181	2	manufacturers	manufacturer	NNS
cord-277909-rn1dow26	181	3	now	now	RB
cord-277909-rn1dow26	181	4	supply	supply	VBP
cord-277909-rn1dow26	181	5	automated	automate	VBN
cord-277909-rn1dow26	181	6	equipment	equipment	NN
cord-277909-rn1dow26	181	7	for	for	IN
cord-277909-rn1dow26	181	8	the	the	DT
cord-277909-rn1dow26	181	9	extraction	extraction	NN
cord-277909-rn1dow26	181	10	of	of	IN
cord-277909-rn1dow26	181	11	nucleic	nucleic	JJ
cord-277909-rn1dow26	181	12	acid	acid	NN
cord-277909-rn1dow26	181	13	from	from	IN
cord-277909-rn1dow26	181	14	diagnostic	diagnostic	JJ
cord-277909-rn1dow26	181	15	samples	sample	NNS
cord-277909-rn1dow26	181	16	(	(	-LRB-
cord-277909-rn1dow26	181	17	Table	Table	NNP
cord-277909-rn1dow26	181	18	4	4	CD
cord-277909-rn1dow26	181	19	)	)	-RRB-
cord-277909-rn1dow26	181	20	.	.	.
cord-277909-rn1dow26	182	1	Some	some	DT
cord-277909-rn1dow26	182	2	manufacturers	manufacturer	NNS
cord-277909-rn1dow26	182	3	provide	provide	VBP
cord-277909-rn1dow26	182	4	open	open	JJ
cord-277909-rn1dow26	182	5	platforms	platform	NNS
cord-277909-rn1dow26	182	6	,	,	,
cord-277909-rn1dow26	182	7	which	which	WDT
cord-277909-rn1dow26	182	8	can	can	MD
cord-277909-rn1dow26	182	9	be	be	VB
cord-277909-rn1dow26	182	10	used	use	VBN
cord-277909-rn1dow26	182	11	with	with	IN
cord-277909-rn1dow26	182	12	other	other	JJ
cord-277909-rn1dow26	182	13	suppliers	supplier	NNS
cord-277909-rn1dow26	182	14	'	'	POS
cord-277909-rn1dow26	182	15	kits	kit	NNS
cord-277909-rn1dow26	182	16	and	and	CC
cord-277909-rn1dow26	182	17	reagents	reagent	NNS
cord-277909-rn1dow26	182	18	,	,	,
cord-277909-rn1dow26	182	19	while	while	IN
cord-277909-rn1dow26	182	20	others	other	NNS
cord-277909-rn1dow26	182	21	provide	provide	VBP
cord-277909-rn1dow26	182	22	complete	complete	JJ
cord-277909-rn1dow26	182	23	extraction	extraction	NN
cord-277909-rn1dow26	182	24	solutions	solution	NNS
cord-277909-rn1dow26	182	25	.	.	.
cord-277909-rn1dow26	183	1	Although	although	IN
cord-277909-rn1dow26	183	2	universal	universal	JJ
cord-277909-rn1dow26	183	3	extraction	extraction	NN
cord-277909-rn1dow26	183	4	kits	kit	NNS
cord-277909-rn1dow26	183	5	(	(	-LRB-
cord-277909-rn1dow26	183	6	DNA	DNA	NNP
cord-277909-rn1dow26	183	7	and	and	CC
cord-277909-rn1dow26	183	8	RNA	RNA	NNP
cord-277909-rn1dow26	183	9	pathogens	pathogen	NNS
cord-277909-rn1dow26	183	10	and	and	CC
cord-277909-rn1dow26	183	11	most	most	JJS
cord-277909-rn1dow26	183	12	specimen	specimen	JJ
cord-277909-rn1dow26	183	13	types	type	NNS
cord-277909-rn1dow26	183	14	)	)	-RRB-
cord-277909-rn1dow26	183	15	are	be	VBP
cord-277909-rn1dow26	183	16	available	available	JJ
cord-277909-rn1dow26	183	17	,	,	,
cord-277909-rn1dow26	183	18	it	-PRON-	PRP
cord-277909-rn1dow26	183	19	should	should	MD
cord-277909-rn1dow26	183	20	also	also	RB
cord-277909-rn1dow26	183	21	be	be	VB
cord-277909-rn1dow26	183	22	noted	note	VBN
cord-277909-rn1dow26	183	23	that	that	IN
cord-277909-rn1dow26	183	24	different	different	JJ
cord-277909-rn1dow26	183	25	kits	kit	NNS
cord-277909-rn1dow26	183	26	can	can	MD
cord-277909-rn1dow26	183	27	be	be	VB
cord-277909-rn1dow26	183	28	used	use	VBN
cord-277909-rn1dow26	183	29	for	for	IN
cord-277909-rn1dow26	183	30	particular	particular	JJ
cord-277909-rn1dow26	183	31	samples	sample	NNS
cord-277909-rn1dow26	183	32	types	type	NNS
cord-277909-rn1dow26	183	33	and	and	CC
cord-277909-rn1dow26	183	34	pathogens	pathogen	NNS
cord-277909-rn1dow26	183	35	(	(	-LRB-
cord-277909-rn1dow26	183	36	e.g.	e.g.	RB
cord-277909-rn1dow26	183	37	,	,	,
cord-277909-rn1dow26	183	38	RNA	RNA	NNP
cord-277909-rn1dow26	183	39	or	or	CC
cord-277909-rn1dow26	183	40	DNA	DNA	NNP
cord-277909-rn1dow26	183	41	)	)	-RRB-
cord-277909-rn1dow26	183	42	and	and	CC
cord-277909-rn1dow26	183	43	may	may	MD
cord-277909-rn1dow26	183	44	be	be	VB
cord-277909-rn1dow26	183	45	more	more	RBR
cord-277909-rn1dow26	183	46	sensitive	sensitive	JJ
cord-277909-rn1dow26	183	47	for	for	IN
cord-277909-rn1dow26	183	48	a	a	DT
cord-277909-rn1dow26	183	49	particular	particular	JJ
cord-277909-rn1dow26	183	50	application	application	NN
cord-277909-rn1dow26	183	51	.	.	.
cord-277909-rn1dow26	184	1	Although	although	IN
cord-277909-rn1dow26	184	2	automated	automated	JJ
cord-277909-rn1dow26	184	3	extraction	extraction	NN
cord-277909-rn1dow26	184	4	has	have	VBZ
cord-277909-rn1dow26	184	5	many	many	JJ
cord-277909-rn1dow26	184	6	advantages	advantage	NNS
cord-277909-rn1dow26	184	7	,	,	,
cord-277909-rn1dow26	184	8	laboratories	laboratory	NNS
cord-277909-rn1dow26	184	9	should	should	MD
cord-277909-rn1dow26	184	10	also	also	RB
cord-277909-rn1dow26	184	11	consider	consider	VB
cord-277909-rn1dow26	184	12	complementing	complement	VBG
cord-277909-rn1dow26	184	13	this	this	DT
cord-277909-rn1dow26	184	14	service	service	NN
cord-277909-rn1dow26	184	15	with	with	IN
cord-277909-rn1dow26	184	16	a	a	DT
cord-277909-rn1dow26	184	17	manual	manual	JJ
cord-277909-rn1dow26	184	18	extraction	extraction	NN
cord-277909-rn1dow26	184	19	system	system	NN
cord-277909-rn1dow26	184	20	.	.	.
cord-277909-rn1dow26	185	1	This	this	DT
cord-277909-rn1dow26	185	2	can	can	MD
cord-277909-rn1dow26	185	3	be	be	VB
cord-277909-rn1dow26	185	4	used	use	VBN
cord-277909-rn1dow26	185	5	for	for	IN
cord-277909-rn1dow26	185	6	testing	test	VBG
cord-277909-rn1dow26	185	7	emergency	emergency	NN
cord-277909-rn1dow26	185	8	samples	sample	NNS
cord-277909-rn1dow26	185	9	that	that	WDT
cord-277909-rn1dow26	185	10	have	have	VBP
cord-277909-rn1dow26	185	11	arrived	arrive	VBN
cord-277909-rn1dow26	185	12	in	in	IN
cord-277909-rn1dow26	185	13	the	the	DT
cord-277909-rn1dow26	185	14	laboratory	laboratory	NN
cord-277909-rn1dow26	185	15	after	after	IN
cord-277909-rn1dow26	185	16	an	an	DT
cord-277909-rn1dow26	185	17	automated	automate	VBN
cord-277909-rn1dow26	185	18	extraction	extraction	NN
cord-277909-rn1dow26	185	19	has	have	VBZ
cord-277909-rn1dow26	185	20	begun	begin	VBN
cord-277909-rn1dow26	185	21	or	or	CC
cord-277909-rn1dow26	185	22	for	for	IN
cord-277909-rn1dow26	185	23	samples	sample	NNS
cord-277909-rn1dow26	185	24	requiring	require	VBG
cord-277909-rn1dow26	185	25	special	special	JJ
cord-277909-rn1dow26	185	26	processing	processing	NN
cord-277909-rn1dow26	185	27	,	,	,
cord-277909-rn1dow26	185	28	not	not	RB
cord-277909-rn1dow26	185	29	suited	suit	VBN
cord-277909-rn1dow26	185	30	for	for	IN
cord-277909-rn1dow26	185	31	automation	automation	NN
cord-277909-rn1dow26	185	32	,	,	,
cord-277909-rn1dow26	185	33	e.g.	e.g.	RB
cord-277909-rn1dow26	185	34	tissue	tissue	NN
cord-277909-rn1dow26	185	35	.	.	.
cord-277909-rn1dow26	186	1	Many	many	JJ
cord-277909-rn1dow26	186	2	suppliers	supplier	NNS
cord-277909-rn1dow26	186	3	also	also	RB
cord-277909-rn1dow26	186	4	supply	supply	VBP
cord-277909-rn1dow26	186	5	automated	automate	VBN
cord-277909-rn1dow26	186	6	liquid	liquid	NN
cord-277909-rn1dow26	186	7	handling	handling	NN
cord-277909-rn1dow26	186	8	equipment	equipment	NN
cord-277909-rn1dow26	186	9	,	,	,
cord-277909-rn1dow26	186	10	which	which	WDT
cord-277909-rn1dow26	186	11	can	can	MD
cord-277909-rn1dow26	186	12	facilitate	facilitate	VB
cord-277909-rn1dow26	186	13	the	the	DT
cord-277909-rn1dow26	186	14	set	set	NN
cord-277909-rn1dow26	186	15	up	up	RP
cord-277909-rn1dow26	186	16	of	of	IN
cord-277909-rn1dow26	186	17	large	large	JJ
cord-277909-rn1dow26	186	18	numbers	number	NNS
cord-277909-rn1dow26	186	19	of	of	IN
cord-277909-rn1dow26	186	20	PCR	PCR	NNP
cord-277909-rn1dow26	186	21	reactions	reaction	NNS
cord-277909-rn1dow26	186	22	.	.	.
cord-277909-rn1dow26	187	1	Traditionally	traditionally	RB
cord-277909-rn1dow26	187	2	most	most	RBS
cord-277909-rn1dow26	187	3	published	publish	VBN
cord-277909-rn1dow26	187	4	and	and	CC
cord-277909-rn1dow26	187	5	in	in	IN
cord-277909-rn1dow26	187	6	-	-	HYPH
cord-277909-rn1dow26	187	7	house	house	NN
cord-277909-rn1dow26	187	8	developed	develop	VBD
cord-277909-rn1dow26	187	9	real	real	JJ
cord-277909-rn1dow26	187	10	time	time	NN
cord-277909-rn1dow26	187	11	PCR	pcr	NN
cord-277909-rn1dow26	187	12	methods	method	NNS
cord-277909-rn1dow26	187	13	consist	consist	VBP
cord-277909-rn1dow26	187	14	of	of	IN
cord-277909-rn1dow26	187	15	the	the	DT
cord-277909-rn1dow26	187	16	following	following	JJ
cord-277909-rn1dow26	187	17	standard	standard	JJ
cord-277909-rn1dow26	187	18	parame	parame	NN
cord-277909-rn1dow26	187	19	-	-	HYPH
cord-277909-rn1dow26	187	20	ters	ter	NNS
cord-277909-rn1dow26	187	21	:	:	:
cord-277909-rn1dow26	187	22	a	a	DT
cord-277909-rn1dow26	187	23	Taq	Taq	NNP
cord-277909-rn1dow26	187	24	DNA	dna	NN
cord-277909-rn1dow26	187	25	polymerase	polymerase	NN
cord-277909-rn1dow26	187	26	activation	activation	NN
cord-277909-rn1dow26	187	27	step	step	NN
cord-277909-rn1dow26	187	28	(	(	-LRB-
cord-277909-rn1dow26	187	29	usually	usually	RB
cord-277909-rn1dow26	187	30	95	95	CD
cord-277909-rn1dow26	187	31	•	•	NN
cord-277909-rn1dow26	187	32	C	c	NN
cord-277909-rn1dow26	187	33	for	for	IN
cord-277909-rn1dow26	187	34	2	2	CD
cord-277909-rn1dow26	187	35	-	-	SYM
cord-277909-rn1dow26	187	36	15	15	CD
cord-277909-rn1dow26	187	37	min	min	NN
cord-277909-rn1dow26	187	38	depending	depend	VBG
cord-277909-rn1dow26	187	39	upon	upon	IN
cord-277909-rn1dow26	187	40	PCR	PCR	NNP
cord-277909-rn1dow26	187	41	kit	kit	NN
cord-277909-rn1dow26	187	42	manufacturer	manufacturer	NN
cord-277909-rn1dow26	187	43	)	)	-RRB-
cord-277909-rn1dow26	187	44	followed	follow	VBN
cord-277909-rn1dow26	187	45	by	by	IN
cord-277909-rn1dow26	187	46	40	40	CD
cord-277909-rn1dow26	187	47	-	-	SYM
cord-277909-rn1dow26	187	48	50	50	CD
cord-277909-rn1dow26	187	49	cycles	cycle	NNS
cord-277909-rn1dow26	187	50	of	of	IN
cord-277909-rn1dow26	187	51	95	95	CD
cord-277909-rn1dow26	188	1	•	•	NNP
cord-277909-rn1dow26	189	1	C	C	NNP
cord-277909-rn1dow26	189	2	denaturation	denaturation	NN
cord-277909-rn1dow26	189	3	for	for	IN
cord-277909-rn1dow26	189	4	15	15	CD
cord-277909-rn1dow26	189	5	-	-	SYM
cord-277909-rn1dow26	189	6	30	30	CD
cord-277909-rn1dow26	189	7	s	s	NNPS
cord-277909-rn1dow26	189	8	and	and	CC
cord-277909-rn1dow26	189	9	an	an	DT
cord-277909-rn1dow26	189	10	annealing	anneal	VBG
cord-277909-rn1dow26	189	11	/	/	SYM
cord-277909-rn1dow26	189	12	extension	extension	NN
cord-277909-rn1dow26	189	13	cycle	cycle	NN
cord-277909-rn1dow26	189	14	of	of	IN
cord-277909-rn1dow26	189	15	60	60	CD
cord-277909-rn1dow26	189	16	•	•	NNS
cord-277909-rn1dow26	189	17	C	C	NNP
cord-277909-rn1dow26	189	18	for	for	IN
cord-277909-rn1dow26	189	19	60	60	CD
cord-277909-rn1dow26	189	20	s.	s.	NN
cord-277909-rn1dow26	190	1	If	if	IN
cord-277909-rn1dow26	190	2	an	an	DT
cord-277909-rn1dow26	190	3	RNA	RNA	NNP
cord-277909-rn1dow26	190	4	virus	virus	NN
cord-277909-rn1dow26	190	5	is	be	VBZ
cord-277909-rn1dow26	190	6	to	to	TO
cord-277909-rn1dow26	190	7	be	be	VB
cord-277909-rn1dow26	190	8	detected	detect	VBN
cord-277909-rn1dow26	190	9	,	,	,
cord-277909-rn1dow26	190	10	an	an	DT
cord-277909-rn1dow26	190	11	additional	additional	JJ
cord-277909-rn1dow26	190	12	30	30	CD
cord-277909-rn1dow26	190	13	min	min	NN
cord-277909-rn1dow26	190	14	reverse	reverse	NN
cord-277909-rn1dow26	190	15	transcription	transcription	NN
cord-277909-rn1dow26	190	16	step	step	NN
cord-277909-rn1dow26	190	17	is	be	VBZ
cord-277909-rn1dow26	190	18	required	require	VBN
cord-277909-rn1dow26	190	19	before	before	IN
cord-277909-rn1dow26	190	20	the	the	DT
cord-277909-rn1dow26	190	21	Taq	Taq	NNP
cord-277909-rn1dow26	190	22	DNA	dna	NN
cord-277909-rn1dow26	190	23	polymerase	polymerase	NN
cord-277909-rn1dow26	190	24	activation	activation	NN
cord-277909-rn1dow26	190	25	.	.	.
cord-277909-rn1dow26	191	1	Overall	overall	RB
cord-277909-rn1dow26	191	2	the	the	DT
cord-277909-rn1dow26	191	3	reaction	reaction	NN
cord-277909-rn1dow26	191	4	run	run	VBN
cord-277909-rn1dow26	191	5	time	time	NN
cord-277909-rn1dow26	191	6	for	for	IN
cord-277909-rn1dow26	191	7	a	a	DT
cord-277909-rn1dow26	191	8	real	real	JJ
cord-277909-rn1dow26	191	9	time	time	NN
cord-277909-rn1dow26	191	10	PCR	PCR	NNP
cord-277909-rn1dow26	191	11	is	be	VBZ
cord-277909-rn1dow26	191	12	between	between	IN
cord-277909-rn1dow26	191	13	80	80	CD
cord-277909-rn1dow26	191	14	and	and	CC
cord-277909-rn1dow26	191	15	100	100	CD
cord-277909-rn1dow26	191	16	min	min	NN
cord-277909-rn1dow26	191	17	.	.	.
cord-277909-rn1dow26	192	1	We	-PRON-	PRP
cord-277909-rn1dow26	192	2	have	have	VBP
cord-277909-rn1dow26	192	3	repeatedly	repeatedly	RB
cord-277909-rn1dow26	192	4	shown	show	VBN
cord-277909-rn1dow26	192	5	using	use	VBG
cord-277909-rn1dow26	192	6	dilution	dilution	NN
cord-277909-rn1dow26	192	7	series	series	NN
cord-277909-rn1dow26	192	8	of	of	IN
cord-277909-rn1dow26	192	9	a	a	DT
cord-277909-rn1dow26	192	10	number	number	NN
cord-277909-rn1dow26	192	11	of	of	IN
cord-277909-rn1dow26	192	12	DNA	DNA	NNP
cord-277909-rn1dow26	192	13	and	and	CC
cord-277909-rn1dow26	192	14	RNA	RNA	NNP
cord-277909-rn1dow26	192	15	viral	viral	JJ
cord-277909-rn1dow26	192	16	pathogens	pathogen	NNS
cord-277909-rn1dow26	192	17	that	that	WDT
cord-277909-rn1dow26	192	18	reducing	reduce	VBG
cord-277909-rn1dow26	192	19	the	the	DT
cord-277909-rn1dow26	192	20	duration	duration	NN
cord-277909-rn1dow26	192	21	of	of	IN
cord-277909-rn1dow26	192	22	the	the	DT
cord-277909-rn1dow26	192	23	reverse	reverse	JJ
cord-277909-rn1dow26	192	24	transcription	transcription	NN
cord-277909-rn1dow26	192	25	step	step	NN
cord-277909-rn1dow26	192	26	,	,	,
cord-277909-rn1dow26	192	27	the	the	DT
cord-277909-rn1dow26	192	28	denaturing	denature	VBG
cord-277909-rn1dow26	192	29	and	and	CC
cord-277909-rn1dow26	192	30	annealing	annealing	NN
cord-277909-rn1dow26	192	31	/	/	SYM
cord-277909-rn1dow26	192	32	extension	extension	NN
cord-277909-rn1dow26	192	33	step	step	NN
cord-277909-rn1dow26	192	34	by	by	IN
cord-277909-rn1dow26	192	35	50	50	CD
cord-277909-rn1dow26	192	36	%	%	NN
cord-277909-rn1dow26	192	37	can	can	MD
cord-277909-rn1dow26	192	38	reduce	reduce	VB
cord-277909-rn1dow26	192	39	the	the	DT
cord-277909-rn1dow26	192	40	reaction	reaction	NN
cord-277909-rn1dow26	192	41	run	run	VBN
cord-277909-rn1dow26	192	42	time	time	NN
cord-277909-rn1dow26	192	43	of	of	IN
cord-277909-rn1dow26	192	44	the	the	DT
cord-277909-rn1dow26	192	45	assay	assay	NN
cord-277909-rn1dow26	192	46	significantly	significantly	RB
cord-277909-rn1dow26	192	47	without	without	IN
cord-277909-rn1dow26	192	48	any	any	DT
cord-277909-rn1dow26	192	49	concurrent	concurrent	JJ
cord-277909-rn1dow26	192	50	loss	loss	NN
cord-277909-rn1dow26	192	51	in	in	IN
cord-277909-rn1dow26	192	52	sensitivity	sensitivity	NN
cord-277909-rn1dow26	192	53	(	(	-LRB-
cord-277909-rn1dow26	192	54	Table	table	NN
cord-277909-rn1dow26	192	55	5	5	CD
cord-277909-rn1dow26	192	56	)	)	-RRB-
cord-277909-rn1dow26	192	57	.	.	.
cord-277909-rn1dow26	193	1	Overall	overall	JJ
cord-277909-rn1dow26	193	2	our	-PRON-	PRP$
cord-277909-rn1dow26	193	3	reaction	reaction	NN
cord-277909-rn1dow26	193	4	run	run	NN
cord-277909-rn1dow26	193	5	time	time	NN
cord-277909-rn1dow26	193	6	has	have	VBZ
cord-277909-rn1dow26	193	7	reduced	reduce	VBN
cord-277909-rn1dow26	193	8	to	to	IN
cord-277909-rn1dow26	193	9	approximately	approximately	RB
cord-277909-rn1dow26	193	10	60	60	CD
cord-277909-rn1dow26	193	11	min	min	NN
cord-277909-rn1dow26	193	12	(	(	-LRB-
cord-277909-rn1dow26	193	13	70	70	CD
cord-277909-rn1dow26	193	14	min	min	NN
cord-277909-rn1dow26	193	15	for	for	IN
cord-277909-rn1dow26	193	16	RT	RT	NNP
cord-277909-rn1dow26	193	17	-	-	HYPH
cord-277909-rn1dow26	193	18	PCR	PCR	NNP
cord-277909-rn1dow26	193	19	)	)	-RRB-
cord-277909-rn1dow26	193	20	,	,	,
cord-277909-rn1dow26	193	21	freeing	free	VBG
cord-277909-rn1dow26	193	22	up	up	RP
cord-277909-rn1dow26	193	23	PCR	PCR	NNP
cord-277909-rn1dow26	193	24	machines	machine	NNS
cord-277909-rn1dow26	193	25	for	for	IN
cord-277909-rn1dow26	193	26	further	further	JJ
cord-277909-rn1dow26	193	27	tests	test	NNS
cord-277909-rn1dow26	193	28	and	and	CC
cord-277909-rn1dow26	193	29	allowing	allow	VBG
cord-277909-rn1dow26	193	30	more	more	JJR
cord-277909-rn1dow26	193	31	testing	testing	NN
cord-277909-rn1dow26	193	32	within	within	IN
cord-277909-rn1dow26	193	33	the	the	DT
cord-277909-rn1dow26	193	34	working	work	VBG
cord-277909-rn1dow26	193	35	day	day	NN
cord-277909-rn1dow26	193	36	.	.	.
cord-277909-rn1dow26	194	1	Most	most	RBS
cord-277909-rn1dow26	194	2	dual	dual	RB
cord-277909-rn1dow26	194	3	labelled	label	VBN
cord-277909-rn1dow26	194	4	probe	probe	NN
cord-277909-rn1dow26	194	5	real	real	JJ
cord-277909-rn1dow26	194	6	time	time	NN
cord-277909-rn1dow26	194	7	PCR	PCR	NNP
cord-277909-rn1dow26	194	8	assays	assay	NNS
cord-277909-rn1dow26	194	9	are	be	VBP
cord-277909-rn1dow26	194	10	designed	design	VBN
cord-277909-rn1dow26	194	11	to	to	TO
cord-277909-rn1dow26	194	12	utilise	utilise	VB
cord-277909-rn1dow26	194	13	the	the	DT
cord-277909-rn1dow26	194	14	same	same	JJ
cord-277909-rn1dow26	194	15	PCR	PCR	NNP
cord-277909-rn1dow26	194	16	parameters	parameter	NNS
cord-277909-rn1dow26	194	17	(	(	-LRB-
cord-277909-rn1dow26	194	18	i.e.	i.e.	FW
cord-277909-rn1dow26	194	19	,	,	,
cord-277909-rn1dow26	194	20	denaturation	denaturation	NN
cord-277909-rn1dow26	194	21	step	step	NN
cord-277909-rn1dow26	194	22	of	of	IN
cord-277909-rn1dow26	194	23	95	95	CD
cord-277909-rn1dow26	195	1	•	•	NNP
cord-277909-rn1dow26	196	1	C	C	NNP
cord-277909-rn1dow26	196	2	for	for	IN
cord-277909-rn1dow26	196	3	30	30	CD
cord-277909-rn1dow26	196	4	s	s	NNS
cord-277909-rn1dow26	196	5	and	and	CC
cord-277909-rn1dow26	196	6	an	an	DT
cord-277909-rn1dow26	196	7	annealing	annealing	NN
cord-277909-rn1dow26	196	8	and	and	CC
cord-277909-rn1dow26	196	9	extention	extention	NN
cord-277909-rn1dow26	196	10	step	step	NN
cord-277909-rn1dow26	196	11	of	of	IN
cord-277909-rn1dow26	196	12	60	60	CD
cord-277909-rn1dow26	196	13	•	•	NNS
cord-277909-rn1dow26	196	14	C	C	NNP
cord-277909-rn1dow26	196	15	for	for	IN
cord-277909-rn1dow26	196	16	60	60	CD
cord-277909-rn1dow26	196	17	s	s	NNS
cord-277909-rn1dow26	196	18	)	)	-RRB-
cord-277909-rn1dow26	196	19	.	.	.
cord-277909-rn1dow26	197	1	Theoretically	theoretically	RB
cord-277909-rn1dow26	197	2	,	,	,
cord-277909-rn1dow26	197	3	multiple	multiple	JJ
cord-277909-rn1dow26	197	4	different	different	JJ
cord-277909-rn1dow26	197	5	real	real	JJ
cord-277909-rn1dow26	197	6	time	time	NN
cord-277909-rn1dow26	197	7	PCR	pcr	NN
cord-277909-rn1dow26	197	8	assays	assay	NNS
cord-277909-rn1dow26	197	9	can	can	MD
cord-277909-rn1dow26	197	10	be	be	VB
cord-277909-rn1dow26	197	11	carried	carry	VBN
cord-277909-rn1dow26	197	12	out	out	RP
cord-277909-rn1dow26	197	13	at	at	IN
cord-277909-rn1dow26	197	14	the	the	DT
cord-277909-rn1dow26	197	15	same	same	JJ
cord-277909-rn1dow26	197	16	time	time	NN
cord-277909-rn1dow26	197	17	on	on	IN
cord-277909-rn1dow26	197	18	the	the	DT
cord-277909-rn1dow26	197	19	sample	sample	NN
cord-277909-rn1dow26	197	20	plate	plate	NN
cord-277909-rn1dow26	197	21	.	.	.
cord-277909-rn1dow26	198	1	We	-PRON-	PRP
cord-277909-rn1dow26	198	2	have	have	VBP
cord-277909-rn1dow26	198	3	also	also	RB
cord-277909-rn1dow26	198	4	shown	show	VBN
cord-277909-rn1dow26	198	5	that	that	IN
cord-277909-rn1dow26	198	6	,	,	,
cord-277909-rn1dow26	198	7	where	where	WRB
cord-277909-rn1dow26	198	8	DNA	DNA	NNP
cord-277909-rn1dow26	198	9	and	and	CC
cord-277909-rn1dow26	198	10	RNA	RNA	NNP
cord-277909-rn1dow26	198	11	reagents	reagent	NNS
cord-277909-rn1dow26	198	12	are	be	VBP
cord-277909-rn1dow26	198	13	purchased	purchase	VBN
cord-277909-rn1dow26	198	14	from	from	IN
cord-277909-rn1dow26	198	15	the	the	DT
cord-277909-rn1dow26	198	16	same	same	JJ
cord-277909-rn1dow26	198	17	supplier	supplier	NN
cord-277909-rn1dow26	198	18	,	,	,
cord-277909-rn1dow26	198	19	and	and	CC
cord-277909-rn1dow26	198	20	therefore	therefore	RB
cord-277909-rn1dow26	198	21	have	have	VBP
cord-277909-rn1dow26	198	22	identical	identical	JJ
cord-277909-rn1dow26	198	23	Taq	Taq	NNP
cord-277909-rn1dow26	198	24	activation	activation	NN
cord-277909-rn1dow26	198	25	requirements	requirement	NNS
cord-277909-rn1dow26	198	26	,	,	,
cord-277909-rn1dow26	198	27	DNA	dna	NN
cord-277909-rn1dow26	198	28	assays	assay	NNS
cord-277909-rn1dow26	198	29	do	do	VBP
cord-277909-rn1dow26	198	30	not	not	RB
cord-277909-rn1dow26	198	31	suffer	suffer	VB
cord-277909-rn1dow26	198	32	any	any	DT
cord-277909-rn1dow26	198	33	loss	loss	NN
cord-277909-rn1dow26	198	34	in	in	IN
cord-277909-rn1dow26	198	35	performance	performance	NN
cord-277909-rn1dow26	198	36	when	when	WRB
cord-277909-rn1dow26	198	37	run	run	VBN
cord-277909-rn1dow26	198	38	through	through	IN
cord-277909-rn1dow26	198	39	RT	RT	NNP
cord-277909-rn1dow26	198	40	-	-	HYPH
cord-277909-rn1dow26	198	41	PCR	PCR	NNP
cord-277909-rn1dow26	198	42	cycling	cycling	NN
cord-277909-rn1dow26	198	43	conditions	condition	NNS
cord-277909-rn1dow26	198	44	.	.	.
cord-277909-rn1dow26	199	1	This	this	DT
cord-277909-rn1dow26	199	2	will	will	MD
cord-277909-rn1dow26	199	3	allow	allow	VB
cord-277909-rn1dow26	199	4	laboratories	laboratory	NNS
cord-277909-rn1dow26	199	5	greater	great	JJR
cord-277909-rn1dow26	199	6	flexibility	flexibility	NN
cord-277909-rn1dow26	199	7	and	and	CC
cord-277909-rn1dow26	199	8	provide	provide	VB
cord-277909-rn1dow26	199	9	a	a	DT
cord-277909-rn1dow26	199	10	rapid	rapid	JJ
cord-277909-rn1dow26	199	11	service	service	NN
cord-277909-rn1dow26	199	12	.	.	.
cord-277909-rn1dow26	200	1	Pre	pre	JJ
cord-277909-rn1dow26	200	2	-	-	JJ
cord-277909-rn1dow26	200	3	prepared	prepared	JJ
cord-277909-rn1dow26	200	4	,	,	,
cord-277909-rn1dow26	200	5	frozen	frozen	JJ
cord-277909-rn1dow26	200	6	real	real	JJ
cord-277909-rn1dow26	200	7	time	time	NN
cord-277909-rn1dow26	200	8	PCR	pcr	NN
cord-277909-rn1dow26	200	9	reagents	reagent	NNS
cord-277909-rn1dow26	200	10	are	be	VBP
cord-277909-rn1dow26	200	11	user	user	NN
cord-277909-rn1dow26	200	12	friendly	friendly	JJ
cord-277909-rn1dow26	200	13	and	and	CC
cord-277909-rn1dow26	200	14	lead	lead	VB
cord-277909-rn1dow26	200	15	to	to	IN
cord-277909-rn1dow26	200	16	reduced	reduce	VBN
cord-277909-rn1dow26	200	17	TRT	TRT	NNP
cord-277909-rn1dow26	200	18	and	and	CC
cord-277909-rn1dow26	200	19	improved	improve	VBN
cord-277909-rn1dow26	200	20	quality	quality	NN
cord-277909-rn1dow26	200	21	control	control	NN
cord-277909-rn1dow26	200	22	(	(	-LRB-
cord-277909-rn1dow26	200	23	QC	QC	NNP
cord-277909-rn1dow26	200	24	)	)	-RRB-
cord-277909-rn1dow26	200	25	when	when	WRB
cord-277909-rn1dow26	200	26	compared	compare	VBN
cord-277909-rn1dow26	200	27	to	to	IN
cord-277909-rn1dow26	200	28	the	the	DT
cord-277909-rn1dow26	200	29	preparation	preparation	NN
cord-277909-rn1dow26	200	30	of	of	IN
cord-277909-rn1dow26	200	31	PCR	PCR	NNP
cord-277909-rn1dow26	200	32	mixes	mix	NNS
cord-277909-rn1dow26	200	33	from	from	IN
cord-277909-rn1dow26	200	34	separate	separate	JJ
cord-277909-rn1dow26	200	35	reagents	reagent	NNS
cord-277909-rn1dow26	200	36	.	.	.
cord-277909-rn1dow26	201	1	We	-PRON-	PRP
cord-277909-rn1dow26	201	2	have	have	VBP
cord-277909-rn1dow26	201	3	assessed	assess	VBN
cord-277909-rn1dow26	201	4	two	two	CD
cord-277909-rn1dow26	201	5	different	different	JJ
cord-277909-rn1dow26	201	6	methods	method	NNS
cord-277909-rn1dow26	201	7	of	of	IN
cord-277909-rn1dow26	201	8	pre	pre	JJ
cord-277909-rn1dow26	201	9	-	-	JJ
cord-277909-rn1dow26	201	10	prepared	prepared	JJ
cord-277909-rn1dow26	201	11	real	real	JJ
cord-277909-rn1dow26	201	12	time	time	NN
cord-277909-rn1dow26	201	13	PCR	PCR	NNP
cord-277909-rn1dow26	201	14	reagents	reagent	NNS
cord-277909-rn1dow26	201	15	:	:	:
cord-277909-rn1dow26	201	16	frozen	frozen	JJ
cord-277909-rn1dow26	201	17	aliquots	aliquot	NNS
cord-277909-rn1dow26	201	18	of	of	IN
cord-277909-rn1dow26	201	19	pooled	pool	VBN
cord-277909-rn1dow26	201	20	primers	primer	NNS
cord-277909-rn1dow26	201	21	and	and	CC
cord-277909-rn1dow26	201	22	probes	probe	NNS
cord-277909-rn1dow26	201	23	,	,	,
cord-277909-rn1dow26	201	24	and	and	CC
cord-277909-rn1dow26	201	25	frozen	frozen	JJ
cord-277909-rn1dow26	201	26	aliquots	aliquot	NNS
cord-277909-rn1dow26	201	27	containing	contain	VBG
cord-277909-rn1dow26	201	28	all	all	DT
cord-277909-rn1dow26	201	29	real	real	JJ
cord-277909-rn1dow26	201	30	time	time	NN
cord-277909-rn1dow26	201	31	PCR	pcr	NN
cord-277909-rn1dow26	201	32	reagents	reagent	NNS
cord-277909-rn1dow26	201	33	.	.	.
cord-277909-rn1dow26	202	1	Both	both	DT
cord-277909-rn1dow26	202	2	systems	system	NNS
cord-277909-rn1dow26	202	3	have	have	VBP
cord-277909-rn1dow26	202	4	been	be	VBN
cord-277909-rn1dow26	202	5	assessed	assess	VBN
cord-277909-rn1dow26	202	6	over	over	IN
cord-277909-rn1dow26	202	7	relatively	relatively	RB
cord-277909-rn1dow26	202	8	short	short	JJ
cord-277909-rn1dow26	202	9	time	time	NN
cord-277909-rn1dow26	202	10	period	period	NN
cord-277909-rn1dow26	202	11	(	(	-LRB-
cord-277909-rn1dow26	202	12	up	up	IN
cord-277909-rn1dow26	202	13	to	to	IN
cord-277909-rn1dow26	202	14	a	a	DT
cord-277909-rn1dow26	202	15	maximum	maximum	NN
cord-277909-rn1dow26	202	16	of	of	IN
cord-277909-rn1dow26	202	17	10	10	CD
cord-277909-rn1dow26	202	18	weeks	week	NNS
cord-277909-rn1dow26	202	19	,	,	,
cord-277909-rn1dow26	202	20	which	which	WDT
cord-277909-rn1dow26	202	21	corresponds	correspond	VBZ
cord-277909-rn1dow26	202	22	to	to	IN
cord-277909-rn1dow26	202	23	the	the	DT
cord-277909-rn1dow26	202	24	maximum	maximum	JJ
cord-277909-rn1dow26	202	25	period	period	NN
cord-277909-rn1dow26	202	26	of	of	IN
cord-277909-rn1dow26	202	27	time	time	NN
cord-277909-rn1dow26	202	28	a	a	DT
cord-277909-rn1dow26	202	29	pool	pool	NN
cord-277909-rn1dow26	202	30	would	would	MD
cord-277909-rn1dow26	202	31	last	last	VB
cord-277909-rn1dow26	202	32	before	before	IN
cord-277909-rn1dow26	202	33	running	run	VBG
cord-277909-rn1dow26	202	34	out	out	RB
cord-277909-rn1dow26	202	35	)	)	-RRB-
cord-277909-rn1dow26	202	36	.	.	.
cord-277909-rn1dow26	203	1	Ideally	ideally	RB
cord-277909-rn1dow26	203	2	these	these	DT
cord-277909-rn1dow26	203	3	would	would	MD
cord-277909-rn1dow26	203	4	have	have	VB
cord-277909-rn1dow26	203	5	been	be	VBN
cord-277909-rn1dow26	203	6	assessed	assess	VBN
cord-277909-rn1dow26	203	7	over	over	IN
cord-277909-rn1dow26	203	8	a	a	DT
cord-277909-rn1dow26	203	9	longer	long	JJR
cord-277909-rn1dow26	203	10	period	period	NN
cord-277909-rn1dow26	203	11	.	.	.
cord-277909-rn1dow26	204	1	We	-PRON-	PRP
cord-277909-rn1dow26	204	2	find	find	VBP
cord-277909-rn1dow26	204	3	that	that	IN
cord-277909-rn1dow26	204	4	the	the	DT
cord-277909-rn1dow26	204	5	pooled	pool	VBN
cord-277909-rn1dow26	204	6	primer	primer	NN
cord-277909-rn1dow26	204	7	and	and	CC
cord-277909-rn1dow26	204	8	probe	probe	NN
cord-277909-rn1dow26	204	9	approach	approach	NN
cord-277909-rn1dow26	204	10	best	good	JJS
cord-277909-rn1dow26	204	11	suits	suit	NNS
cord-277909-rn1dow26	204	12	seasonal	seasonal	JJ
cord-277909-rn1dow26	204	13	assays	assay	NNS
cord-277909-rn1dow26	204	14	such	such	JJ
cord-277909-rn1dow26	204	15	as	as	IN
cord-277909-rn1dow26	204	16	those	those	DT
cord-277909-rn1dow26	204	17	for	for	IN
cord-277909-rn1dow26	204	18	respiratory	respiratory	JJ
cord-277909-rn1dow26	204	19	pathogens	pathogen	NNS
cord-277909-rn1dow26	204	20	,	,	,
cord-277909-rn1dow26	204	21	whereas	whereas	IN
cord-277909-rn1dow26	204	22	the	the	DT
cord-277909-rn1dow26	204	23	latter	latter	JJ
cord-277909-rn1dow26	204	24	approach	approach	NN
cord-277909-rn1dow26	204	25	is	be	VBZ
cord-277909-rn1dow26	204	26	more	more	RBR
cord-277909-rn1dow26	204	27	suited	suited	JJ
cord-277909-rn1dow26	204	28	to	to	IN
cord-277909-rn1dow26	204	29	assays	assay	NNS
cord-277909-rn1dow26	204	30	which	which	WDT
cord-277909-rn1dow26	204	31	are	be	VBP
cord-277909-rn1dow26	204	32	performed	perform	VBN
cord-277909-rn1dow26	204	33	regularly	regularly	RB
cord-277909-rn1dow26	204	34	throughout	throughout	IN
cord-277909-rn1dow26	204	35	the	the	DT
cord-277909-rn1dow26	204	36	year	year	NN
cord-277909-rn1dow26	204	37	on	on	IN
cord-277909-rn1dow26	204	38	a	a	DT
cord-277909-rn1dow26	204	39	standard	standard	JJ
cord-277909-rn1dow26	204	40	number	number	NN
cord-277909-rn1dow26	204	41	of	of	IN
cord-277909-rn1dow26	204	42	samples	sample	NNS
cord-277909-rn1dow26	204	43	.	.	.
cord-277909-rn1dow26	205	1	The	the	DT
cord-277909-rn1dow26	205	2	advantages	advantage	NNS
cord-277909-rn1dow26	205	3	and	and	CC
cord-277909-rn1dow26	205	4	disadvantages	disadvantage	NNS
cord-277909-rn1dow26	205	5	are	be	VBP
cord-277909-rn1dow26	205	6	listed	list	VBN
cord-277909-rn1dow26	205	7	in	in	IN
cord-277909-rn1dow26	205	8	Table	Table	NNP
cord-277909-rn1dow26	205	9	6	6	CD
cord-277909-rn1dow26	205	10	.	.	.
cord-277909-rn1dow26	206	1	We	-PRON-	PRP
cord-277909-rn1dow26	206	2	have	have	VBP
cord-277909-rn1dow26	206	3	introduced	introduce	VBN
cord-277909-rn1dow26	206	4	pooled	pool	VBN
cord-277909-rn1dow26	206	5	primer	primer	NN
cord-277909-rn1dow26	206	6	and	and	CC
cord-277909-rn1dow26	206	7	probes	probe	NNS
cord-277909-rn1dow26	206	8	for	for	IN
cord-277909-rn1dow26	206	9	the	the	DT
cord-277909-rn1dow26	206	10	majority	majority	NN
cord-277909-rn1dow26	206	11	of	of	IN
cord-277909-rn1dow26	206	12	our	-PRON-	PRP$
cord-277909-rn1dow26	206	13	routine	routine	JJ
cord-277909-rn1dow26	206	14	DNA	dna	NN
cord-277909-rn1dow26	206	15	and	and	CC
cord-277909-rn1dow26	206	16	RNA	RNA	NNP
cord-277909-rn1dow26	206	17	tests	test	NNS
cord-277909-rn1dow26	206	18	.	.	.
cord-277909-rn1dow26	207	1	This	this	DT
cord-277909-rn1dow26	207	2	has	have	VBZ
cord-277909-rn1dow26	207	3	proved	prove	VBN
cord-277909-rn1dow26	207	4	especially	especially	RB
cord-277909-rn1dow26	207	5	useful	useful	JJ
cord-277909-rn1dow26	207	6	for	for	IN
cord-277909-rn1dow26	207	7	our	-PRON-	PRP$
cord-277909-rn1dow26	207	8	high	high	JJ
cord-277909-rn1dow26	207	9	throughput	throughput	NN
cord-277909-rn1dow26	207	10	assays	assay	NNS
cord-277909-rn1dow26	207	11	such	such	JJ
cord-277909-rn1dow26	207	12	as	as	IN
cord-277909-rn1dow26	207	13	the	the	DT
cord-277909-rn1dow26	207	14	'	'	``
cord-277909-rn1dow26	207	15	Respiratory	Respiratory	NNP
cord-277909-rn1dow26	207	16	Screen	Screen	NNP
cord-277909-rn1dow26	207	17	'	'	''
cord-277909-rn1dow26	207	18	,	,	,
cord-277909-rn1dow26	207	19	which	which	WDT
cord-277909-rn1dow26	207	20	consists	consist	VBZ
cord-277909-rn1dow26	207	21	of	of	IN
cord-277909-rn1dow26	207	22	five	five	CD
cord-277909-rn1dow26	207	23	triplex	triplex	JJ
cord-277909-rn1dow26	207	24	real	real	JJ
cord-277909-rn1dow26	207	25	time	time	NN
cord-277909-rn1dow26	207	26	RT	RT	NNP
cord-277909-rn1dow26	207	27	-	-	HYPH
cord-277909-rn1dow26	207	28	PCR	PCR	NNP
cord-277909-rn1dow26	207	29	assays	assay	NNS
cord-277909-rn1dow26	207	30	.	.	.
cord-277909-rn1dow26	208	1	For	for	IN
cord-277909-rn1dow26	208	2	each	each	DT
cord-277909-rn1dow26	208	3	multiplex	multiplex	JJ
cord-277909-rn1dow26	208	4	assay	assay	NN
cord-277909-rn1dow26	208	5	,	,	,
cord-277909-rn1dow26	208	6	the	the	DT
cord-277909-rn1dow26	208	7	operator	operator	NN
cord-277909-rn1dow26	208	8	needs	need	VBZ
cord-277909-rn1dow26	208	9	only	only	RB
cord-277909-rn1dow26	208	10	to	to	TO
cord-277909-rn1dow26	208	11	mix	mix	VB
cord-277909-rn1dow26	208	12	three	three	CD
cord-277909-rn1dow26	208	13	tubes	tube	NNS
cord-277909-rn1dow26	208	14	containing	contain	VBG
cord-277909-rn1dow26	208	15	pre	pre	JJ
cord-277909-rn1dow26	208	16	-	-	JJ
cord-277909-rn1dow26	208	17	aliquotted	aliquotte	VBN
cord-277909-rn1dow26	208	18	reagents	reagent	NNS
cord-277909-rn1dow26	208	19	:	:	:
cord-277909-rn1dow26	208	20	an	an	DT
cord-277909-rn1dow26	208	21	aliquot	aliquot	NN
cord-277909-rn1dow26	208	22	of	of	IN
cord-277909-rn1dow26	208	23	mastermix	mastermix	NNP
cord-277909-rn1dow26	208	24	containing	contain	VBG
cord-277909-rn1dow26	208	25	ROX	ROX	NNP
cord-277909-rn1dow26	208	26	reference	reference	NN
cord-277909-rn1dow26	208	27	dye	dye	NN
cord-277909-rn1dow26	208	28	,	,	,
cord-277909-rn1dow26	208	29	one	one	CD
cord-277909-rn1dow26	208	30	containing	contain	VBG
cord-277909-rn1dow26	208	31	enzyme	enzyme	NN
cord-277909-rn1dow26	208	32	mix	mix	NN
cord-277909-rn1dow26	208	33	(	(	-LRB-
cord-277909-rn1dow26	208	34	RT	rt	NN
cord-277909-rn1dow26	208	35	+	+	CC
cord-277909-rn1dow26	208	36	Taq	Taq	NNP
cord-277909-rn1dow26	208	37	)	)	-RRB-
cord-277909-rn1dow26	208	38	,	,	,
cord-277909-rn1dow26	208	39	and	and	CC
cord-277909-rn1dow26	208	40	an	an	DT
cord-277909-rn1dow26	208	41	aliquot	aliquot	NN
cord-277909-rn1dow26	208	42	of	of	IN
cord-277909-rn1dow26	208	43	primer	primer	NN
cord-277909-rn1dow26	208	44	probe	probe	NN
cord-277909-rn1dow26	208	45	pool	pool	NN
cord-277909-rn1dow26	208	46	(	(	-LRB-
cord-277909-rn1dow26	208	47	containing	contain	VBG
cord-277909-rn1dow26	208	48	three	three	CD
cord-277909-rn1dow26	208	49	sets	set	NNS
cord-277909-rn1dow26	208	50	of	of	IN
cord-277909-rn1dow26	208	51	primers	primer	NNS
cord-277909-rn1dow26	208	52	and	and	CC
cord-277909-rn1dow26	208	53	probes	probe	NNS
cord-277909-rn1dow26	208	54	,	,	,
cord-277909-rn1dow26	208	55	and	and	CC
cord-277909-rn1dow26	208	56	sufficient	sufficient	JJ
cord-277909-rn1dow26	208	57	water	water	NN
cord-277909-rn1dow26	208	58	)	)	-RRB-
cord-277909-rn1dow26	208	59	.	.	.
cord-277909-rn1dow26	209	1	In	in	IN
cord-277909-rn1dow26	209	2	this	this	DT
cord-277909-rn1dow26	209	3	way	way	NN
cord-277909-rn1dow26	209	4	,	,	,
cord-277909-rn1dow26	209	5	mastermix	mastermix	NNP
cord-277909-rn1dow26	209	6	can	can	MD
cord-277909-rn1dow26	209	7	be	be	VB
cord-277909-rn1dow26	209	8	prepared	prepare	VBN
cord-277909-rn1dow26	209	9	rapidly	rapidly	RB
cord-277909-rn1dow26	209	10	.	.	.
cord-277909-rn1dow26	210	1	The	the	DT
cord-277909-rn1dow26	210	2	reagents	reagent	NNS
cord-277909-rn1dow26	210	3	have	have	VBP
cord-277909-rn1dow26	210	4	been	be	VBN
cord-277909-rn1dow26	210	5	carefully	carefully	RB
cord-277909-rn1dow26	210	6	quality	quality	NN
cord-277909-rn1dow26	210	7	controlled	control	VBN
cord-277909-rn1dow26	210	8	and	and	CC
cord-277909-rn1dow26	210	9	the	the	DT
cord-277909-rn1dow26	210	10	possibility	possibility	NN
cord-277909-rn1dow26	210	11	of	of	IN
cord-277909-rn1dow26	210	12	pipetting	pipetting	NN
cord-277909-rn1dow26	210	13	or	or	CC
cord-277909-rn1dow26	210	14	calculation	calculation	NN
cord-277909-rn1dow26	210	15	errors	error	NNS
cord-277909-rn1dow26	210	16	at	at	IN
cord-277909-rn1dow26	210	17	the	the	DT
cord-277909-rn1dow26	210	18	time	time	NN
cord-277909-rn1dow26	210	19	of	of	IN
cord-277909-rn1dow26	210	20	preparation	preparation	NN
cord-277909-rn1dow26	210	21	is	be	VBZ
cord-277909-rn1dow26	210	22	reduced	reduce	VBN
cord-277909-rn1dow26	210	23	.	.	.
cord-277909-rn1dow26	211	1	The	the	DT
cord-277909-rn1dow26	211	2	production	production	NN
cord-277909-rn1dow26	211	3	of	of	IN
cord-277909-rn1dow26	211	4	a	a	DT
cord-277909-rn1dow26	211	5	large	large	JJ
cord-277909-rn1dow26	211	6	number	number	NN
cord-277909-rn1dow26	211	7	of	of	IN
cord-277909-rn1dow26	211	8	aliquots	aliquot	NNS
cord-277909-rn1dow26	211	9	at	at	IN
cord-277909-rn1dow26	211	10	the	the	DT
cord-277909-rn1dow26	211	11	same	same	JJ
cord-277909-rn1dow26	211	12	time	time	NN
cord-277909-rn1dow26	211	13	(	(	-LRB-
cord-277909-rn1dow26	211	14	sufficient	sufficient	JJ
cord-277909-rn1dow26	211	15	for	for	IN
cord-277909-rn1dow26	211	16	approximately	approximately	RB
cord-277909-rn1dow26	211	17	3000	3000	CD
cord-277909-rn1dow26	211	18	tests	test	NNS
cord-277909-rn1dow26	211	19	)	)	-RRB-
cord-277909-rn1dow26	211	20	also	also	RB
cord-277909-rn1dow26	211	21	facilitates	facilitate	VBZ
cord-277909-rn1dow26	211	22	inter	inter	JJ
cord-277909-rn1dow26	211	23	-	-	JJ
cord-277909-rn1dow26	211	24	run	run	JJ
cord-277909-rn1dow26	211	25	reproducibility	reproducibility	NN
cord-277909-rn1dow26	211	26	and	and	CC
cord-277909-rn1dow26	211	27	assists	assist	VBZ
cord-277909-rn1dow26	211	28	in	in	IN
cord-277909-rn1dow26	211	29	maintaining	maintain	VBG
cord-277909-rn1dow26	211	30	the	the	DT
cord-277909-rn1dow26	211	31	quality	quality	NN
cord-277909-rn1dow26	211	32	of	of	IN
cord-277909-rn1dow26	211	33	the	the	DT
cord-277909-rn1dow26	211	34	results	result	NNS
cord-277909-rn1dow26	211	35	.	.	.
cord-277909-rn1dow26	212	1	While	while	IN
cord-277909-rn1dow26	212	2	some	some	DT
cord-277909-rn1dow26	212	3	mix	mix	NN
cord-277909-rn1dow26	212	4	is	be	VBZ
cord-277909-rn1dow26	212	5	unavoidably	unavoidably	RB
cord-277909-rn1dow26	212	6	wasted	waste	VBN
cord-277909-rn1dow26	212	7	,	,	,
cord-277909-rn1dow26	212	8	the	the	DT
cord-277909-rn1dow26	212	9	time	time	NN
cord-277909-rn1dow26	212	10	saved	save	VBD
cord-277909-rn1dow26	212	11	and	and	CC
cord-277909-rn1dow26	212	12	the	the	DT
cord-277909-rn1dow26	212	13	reduced	reduced	JJ
cord-277909-rn1dow26	212	14	number	number	NN
cord-277909-rn1dow26	212	15	of	of	IN
cord-277909-rn1dow26	212	16	failed	fail	VBN
cord-277909-rn1dow26	212	17	runs	run	NNS
cord-277909-rn1dow26	212	18	compensates	compensate	VBZ
cord-277909-rn1dow26	212	19	for	for	IN
cord-277909-rn1dow26	212	20	this	this	DT
cord-277909-rn1dow26	212	21	cost	cost	NN
cord-277909-rn1dow26	212	22	,	,	,
cord-277909-rn1dow26	212	23	and	and	CC
cord-277909-rn1dow26	212	24	during	during	IN
cord-277909-rn1dow26	212	25	the	the	DT
cord-277909-rn1dow26	212	26	summer	summer	NN
cord-277909-rn1dow26	212	27	months	month	NNS
cord-277909-rn1dow26	212	28	when	when	WRB
cord-277909-rn1dow26	212	29	sample	sample	NN
cord-277909-rn1dow26	212	30	numbers	number	NNS
cord-277909-rn1dow26	212	31	are	be	VBP
cord-277909-rn1dow26	212	32	much	much	RB
cord-277909-rn1dow26	212	33	reduced	reduce	VBN
cord-277909-rn1dow26	212	34	,	,	,
cord-277909-rn1dow26	212	35	smaller	small	JJR
cord-277909-rn1dow26	212	36	aliquots	aliquot	NNS
cord-277909-rn1dow26	212	37	can	can	MD
cord-277909-rn1dow26	212	38	be	be	VB
cord-277909-rn1dow26	212	39	prepared	prepare	VBN
cord-277909-rn1dow26	212	40	.	.	.
cord-277909-rn1dow26	213	1	Table	table	NN
cord-277909-rn1dow26	213	2	7	7	CD
cord-277909-rn1dow26	213	3	shows	show	VBZ
cord-277909-rn1dow26	213	4	the	the	DT
cord-277909-rn1dow26	213	5	CT	CT	NNP
cord-277909-rn1dow26	213	6	values	value	NNS
cord-277909-rn1dow26	213	7	obtained	obtain	VBN
cord-277909-rn1dow26	213	8	from	from	IN
cord-277909-rn1dow26	213	9	the	the	DT
cord-277909-rn1dow26	213	10	coronavirus	coronavirus	NN
cord-277909-rn1dow26	213	11	triplex	triplex	NN
cord-277909-rn1dow26	213	12	assay	assay	NN
cord-277909-rn1dow26	213	13	using	use	VBG
cord-277909-rn1dow26	213	14	pooled	pool	VBN
cord-277909-rn1dow26	213	15	primers	primer	NNS
cord-277909-rn1dow26	213	16	and	and	CC
cord-277909-rn1dow26	213	17	probed	probe	VBN
cord-277909-rn1dow26	213	18	stored	store	VBN
cord-277909-rn1dow26	213	19	for	for	IN
cord-277909-rn1dow26	213	20	up	up	IN
cord-277909-rn1dow26	213	21	to	to	TO
cord-277909-rn1dow26	213	22	6	6	CD
cord-277909-rn1dow26	213	23	weeks	week	NNS
cord-277909-rn1dow26	213	24	,	,	,
cord-277909-rn1dow26	213	25	demonstrating	demonstrate	VBG
cord-277909-rn1dow26	213	26	the	the	DT
cord-277909-rn1dow26	213	27	stability	stability	NN
cord-277909-rn1dow26	213	28	of	of	IN
cord-277909-rn1dow26	213	29	the	the	DT
cord-277909-rn1dow26	213	30	reagents	reagent	NNS
cord-277909-rn1dow26	213	31	when	when	WRB
cord-277909-rn1dow26	213	32	stored	store	VBN
cord-277909-rn1dow26	213	33	at	at	IN
cord-277909-rn1dow26	213	34	−20	−20	NNP
cord-277909-rn1dow26	213	35	•	•	NNP
cord-277909-rn1dow26	213	36	C.	C.	NNP
cord-277909-rn1dow26	214	1	We	-PRON-	PRP
cord-277909-rn1dow26	214	2	have	have	VBP
cord-277909-rn1dow26	214	3	now	now	RB
cord-277909-rn1dow26	214	4	been	be	VBN
cord-277909-rn1dow26	214	5	using	use	VBG
cord-277909-rn1dow26	214	6	the	the	DT
cord-277909-rn1dow26	214	7	same	same	JJ
cord-277909-rn1dow26	214	8	lot	lot	NN
cord-277909-rn1dow26	214	9	number	number	NN
cord-277909-rn1dow26	214	10	of	of	IN
cord-277909-rn1dow26	214	11	pooled	pool	VBN
cord-277909-rn1dow26	214	12	primer	primer	NN
cord-277909-rn1dow26	214	13	-	-	HYPH
cord-277909-rn1dow26	214	14	probe	probe	NN
cord-277909-rn1dow26	214	15	for	for	IN
cord-277909-rn1dow26	214	16	the	the	DT
cord-277909-rn1dow26	214	17	coronavirus	coronavirus	NN
cord-277909-rn1dow26	214	18	assay	assay	NN
cord-277909-rn1dow26	214	19	for	for	IN
cord-277909-rn1dow26	214	20	in	in	IN
cord-277909-rn1dow26	214	21	excess	excess	NN
cord-277909-rn1dow26	214	22	of	of	IN
cord-277909-rn1dow26	214	23	10	10	CD
cord-277909-rn1dow26	214	24	weeks	week	NNS
cord-277909-rn1dow26	214	25	without	without	IN
cord-277909-rn1dow26	214	26	loss	loss	NN
cord-277909-rn1dow26	214	27	of	of	IN
cord-277909-rn1dow26	214	28	performance	performance	NN
cord-277909-rn1dow26	214	29	.	.	.
cord-277909-rn1dow26	215	1	With	with	IN
cord-277909-rn1dow26	215	2	this	this	DT
cord-277909-rn1dow26	215	3	system	system	NN
cord-277909-rn1dow26	215	4	and	and	CC
cord-277909-rn1dow26	215	5	pooled	pool	VBN
cord-277909-rn1dow26	215	6	controls	control	NNS
cord-277909-rn1dow26	215	7	(	(	-LRB-
cord-277909-rn1dow26	215	8	see	see	VB
cord-277909-rn1dow26	215	9	below	below	RB
cord-277909-rn1dow26	215	10	)	)	-RRB-
cord-277909-rn1dow26	215	11	in	in	IN
cord-277909-rn1dow26	215	12	use	use	NN
cord-277909-rn1dow26	215	13	,	,	,
cord-277909-rn1dow26	215	14	we	-PRON-	PRP
cord-277909-rn1dow26	215	15	are	be	VBP
cord-277909-rn1dow26	215	16	now	now	RB
cord-277909-rn1dow26	215	17	able	able	JJ
cord-277909-rn1dow26	215	18	to	to	TO
cord-277909-rn1dow26	215	19	provide	provide	VB
cord-277909-rn1dow26	215	20	an	an	DT
cord-277909-rn1dow26	215	21	efficient	efficient	JJ
cord-277909-rn1dow26	215	22	and	and	CC
cord-277909-rn1dow26	215	23	reliable	reliable	JJ
cord-277909-rn1dow26	215	24	.	.	.
cord-277909-rn1dow26	216	1	3.6.2	3.6.2	CD
cord-277909-rn1dow26	216	2	.	.	.
cord-277909-rn1dow26	217	1	Frozen	frozen	JJ
cord-277909-rn1dow26	217	2	pools	pool	NNS
cord-277909-rn1dow26	217	3	of	of	IN
cord-277909-rn1dow26	217	4	primers	primer	NNS
cord-277909-rn1dow26	217	5	,	,	,
cord-277909-rn1dow26	217	6	probes	probe	NNS
cord-277909-rn1dow26	217	7	,	,	,
cord-277909-rn1dow26	217	8	mastermix	mastermix	NNP
cord-277909-rn1dow26	217	9	and	and	CC
cord-277909-rn1dow26	217	10	enzyme	enzyme	NNP
cord-277909-rn1dow26	218	1	The	the	DT
cord-277909-rn1dow26	218	2	use	use	NN
cord-277909-rn1dow26	218	3	of	of	IN
cord-277909-rn1dow26	218	4	aliquots	aliquot	NNS
cord-277909-rn1dow26	218	5	of	of	IN
cord-277909-rn1dow26	218	6	frozen	frozen	JJ
cord-277909-rn1dow26	218	7	mastermix	mastermix	NN
cord-277909-rn1dow26	218	8	(	(	-LRB-
cord-277909-rn1dow26	218	9	containing	contain	VBG
cord-277909-rn1dow26	218	10	all	all	DT
cord-277909-rn1dow26	218	11	PCR	PCR	NNP
cord-277909-rn1dow26	218	12	reagents	reagent	NNS
cord-277909-rn1dow26	218	13	except	except	IN
cord-277909-rn1dow26	218	14	template	template	NN
cord-277909-rn1dow26	218	15	)	)	-RRB-
cord-277909-rn1dow26	218	16	is	be	VBZ
cord-277909-rn1dow26	218	17	an	an	DT
cord-277909-rn1dow26	218	18	alternative	alternative	NN
cord-277909-rn1dow26	218	19	to	to	IN
cord-277909-rn1dow26	218	20	frozen	frozen	JJ
cord-277909-rn1dow26	218	21	primer	primer	NN
cord-277909-rn1dow26	218	22	and	and	CC
cord-277909-rn1dow26	218	23	probe	probe	NN
cord-277909-rn1dow26	218	24	aliquots	aliquot	NNS
cord-277909-rn1dow26	218	25	described	describe	VBN
cord-277909-rn1dow26	218	26	above	above	RB
cord-277909-rn1dow26	218	27	.	.	.
cord-277909-rn1dow26	219	1	The	the	DT
cord-277909-rn1dow26	219	2	laboratory	laboratory	NN
cord-277909-rn1dow26	219	3	user	user	NN
cord-277909-rn1dow26	219	4	need	nee	MD
cord-277909-rn1dow26	219	5	only	only	RB
cord-277909-rn1dow26	219	6	remove	remove	VB
cord-277909-rn1dow26	219	7	the	the	DT
cord-277909-rn1dow26	219	8	desired	desire	VBN
cord-277909-rn1dow26	219	9	number	number	NN
cord-277909-rn1dow26	219	10	of	of	IN
cord-277909-rn1dow26	219	11	aliquots	aliquot	NNS
cord-277909-rn1dow26	219	12	(	(	-LRB-
cord-277909-rn1dow26	219	13	or	or	CC
cord-277909-rn1dow26	219	14	plates	plate	NNS
cord-277909-rn1dow26	219	15	if	if	IN
cord-277909-rn1dow26	219	16	frozen	freeze	VBN
cord-277909-rn1dow26	219	17	in	in	IN
cord-277909-rn1dow26	219	18	this	this	DT
cord-277909-rn1dow26	219	19	format	format	NN
cord-277909-rn1dow26	219	20	)	)	-RRB-
cord-277909-rn1dow26	219	21	,	,	,
cord-277909-rn1dow26	219	22	defrost	defrost	VB
cord-277909-rn1dow26	219	23	and	and	CC
cord-277909-rn1dow26	219	24	then	then	RB
cord-277909-rn1dow26	219	25	add	add	VB
cord-277909-rn1dow26	219	26	the	the	DT
cord-277909-rn1dow26	219	27	template	template	NN
cord-277909-rn1dow26	219	28	to	to	TO
cord-277909-rn1dow26	219	29	be	be	VB
cord-277909-rn1dow26	219	30	tested	test	VBN
cord-277909-rn1dow26	219	31	.	.	.
cord-277909-rn1dow26	220	1	Frozen	frozen	JJ
cord-277909-rn1dow26	220	2	aliquots	aliquot	NNS
cord-277909-rn1dow26	220	3	are	be	VBP
cord-277909-rn1dow26	220	4	easier	easy	JJR
cord-277909-rn1dow26	220	5	to	to	TO
cord-277909-rn1dow26	220	6	use	use	VB
cord-277909-rn1dow26	220	7	than	than	IN
cord-277909-rn1dow26	220	8	the	the	DT
cord-277909-rn1dow26	220	9	pooled	pool	VBN
cord-277909-rn1dow26	220	10	primer	primer	NN
cord-277909-rn1dow26	220	11	and	and	CC
cord-277909-rn1dow26	220	12	probe	probe	NN
cord-277909-rn1dow26	220	13	method	method	NN
cord-277909-rn1dow26	220	14	,	,	,
cord-277909-rn1dow26	220	15	facilitate	facilitate	VBP
cord-277909-rn1dow26	220	16	rigorous	rigorous	JJ
cord-277909-rn1dow26	220	17	quality	quality	NN
cord-277909-rn1dow26	220	18	control	control	NN
cord-277909-rn1dow26	220	19	and	and	CC
cord-277909-rn1dow26	220	20	reduce	reduce	VB
cord-277909-rn1dow26	220	21	the	the	DT
cord-277909-rn1dow26	220	22	overall	overall	JJ
cord-277909-rn1dow26	220	23	turn	turn	NN
cord-277909-rn1dow26	220	24	around	around	IN
cord-277909-rn1dow26	220	25	times	time	NNS
cord-277909-rn1dow26	220	26	.	.	.
cord-277909-rn1dow26	221	1	However	however	RB
cord-277909-rn1dow26	221	2	,	,	,
cord-277909-rn1dow26	221	3	they	-PRON-	PRP
cord-277909-rn1dow26	221	4	are	be	VBP
cord-277909-rn1dow26	221	5	less	less	RBR
cord-277909-rn1dow26	221	6	flexible	flexible	JJ
cord-277909-rn1dow26	221	7	than	than	IN
cord-277909-rn1dow26	221	8	the	the	DT
cord-277909-rn1dow26	221	9	primer	primer	NN
cord-277909-rn1dow26	221	10	probe	probe	NN
cord-277909-rn1dow26	221	11	aliquot	aliquot	NN
cord-277909-rn1dow26	221	12	system	system	NN
cord-277909-rn1dow26	221	13	and	and	CC
cord-277909-rn1dow26	221	14	wastage	wastage	NN
cord-277909-rn1dow26	221	15	will	will	MD
cord-277909-rn1dow26	221	16	be	be	VB
cord-277909-rn1dow26	221	17	more	more	RBR
cord-277909-rn1dow26	221	18	expensive	expensive	JJ
cord-277909-rn1dow26	221	19	as	as	IN
cord-277909-rn1dow26	221	20	it	-PRON-	PRP
cord-277909-rn1dow26	221	21	includes	include	VBZ
cord-277909-rn1dow26	221	22	enzyme	enzyme	NN
cord-277909-rn1dow26	221	23	.	.	.
cord-277909-rn1dow26	222	1	Furthermore	furthermore	RB
cord-277909-rn1dow26	222	2	,	,	,
cord-277909-rn1dow26	222	3	any	any	DT
cord-277909-rn1dow26	222	4	mistakes	mistake	NNS
cord-277909-rn1dow26	222	5	in	in	IN
cord-277909-rn1dow26	222	6	the	the	DT
cord-277909-rn1dow26	222	7	making	making	NN
cord-277909-rn1dow26	222	8	up	up	RP
cord-277909-rn1dow26	222	9	of	of	IN
cord-277909-rn1dow26	222	10	the	the	DT
cord-277909-rn1dow26	222	11	aliquots	aliquot	NNS
cord-277909-rn1dow26	222	12	will	will	MD
cord-277909-rn1dow26	222	13	result	result	VB
cord-277909-rn1dow26	222	14	in	in	IN
cord-277909-rn1dow26	222	15	the	the	DT
cord-277909-rn1dow26	222	16	loss	loss	NN
cord-277909-rn1dow26	222	17	of	of	IN
cord-277909-rn1dow26	222	18	primers	primer	NNS
cord-277909-rn1dow26	222	19	and	and	CC
cord-277909-rn1dow26	222	20	probe	probe	NN
cord-277909-rn1dow26	222	21	and	and	CC
cord-277909-rn1dow26	222	22	expensive	expensive	JJ
cord-277909-rn1dow26	222	23	mastermix	mastermix	NNS
cord-277909-rn1dow26	222	24	.	.	.
cord-277909-rn1dow26	223	1	We	-PRON-	PRP
cord-277909-rn1dow26	223	2	have	have	VBP
cord-277909-rn1dow26	223	3	shown	show	VBN
cord-277909-rn1dow26	223	4	,	,	,
cord-277909-rn1dow26	223	5	using	use	VBG
cord-277909-rn1dow26	223	6	positive	positive	JJ
cord-277909-rn1dow26	223	7	controls	control	NNS
cord-277909-rn1dow26	223	8	,	,	,
cord-277909-rn1dow26	223	9	that	that	IN
cord-277909-rn1dow26	223	10	both	both	CC
cord-277909-rn1dow26	223	11	RNA	RNA	NNP
cord-277909-rn1dow26	223	12	and	and	CC
cord-277909-rn1dow26	223	13	DNA	DNA	NNP
cord-277909-rn1dow26	223	14	mastermix	mastermix	NN
cord-277909-rn1dow26	223	15	from	from	IN
cord-277909-rn1dow26	223	16	a	a	DT
cord-277909-rn1dow26	223	17	number	number	NN
cord-277909-rn1dow26	223	18	of	of	IN
cord-277909-rn1dow26	223	19	companies	company	NNS
cord-277909-rn1dow26	223	20	(	(	-LRB-
cord-277909-rn1dow26	223	21	Applied	Applied	NNP
cord-277909-rn1dow26	223	22	Biosystems	Biosystems	NNP
cord-277909-rn1dow26	223	23	,	,	,
cord-277909-rn1dow26	223	24	Invitrogen	Invitrogen	NNP
cord-277909-rn1dow26	223	25	,	,	,
cord-277909-rn1dow26	223	26	and	and	CC
cord-277909-rn1dow26	223	27	Qiagen	Qiagen	NNP
cord-277909-rn1dow26	223	28	)	)	-RRB-
cord-277909-rn1dow26	223	29	can	can	MD
cord-277909-rn1dow26	223	30	be	be	VB
cord-277909-rn1dow26	223	31	frozen	freeze	VBN
cord-277909-rn1dow26	223	32	for	for	IN
cord-277909-rn1dow26	223	33	at	at	RB
cord-277909-rn1dow26	223	34	least	least	RBS
cord-277909-rn1dow26	223	35	1	1	CD
cord-277909-rn1dow26	223	36	month	month	NN
cord-277909-rn1dow26	223	37	with	with	IN
cord-277909-rn1dow26	223	38	no	no	DT
cord-277909-rn1dow26	223	39	loss	loss	NN
cord-277909-rn1dow26	223	40	of	of	IN
cord-277909-rn1dow26	223	41	sensitivity	sensitivity	NN
cord-277909-rn1dow26	223	42	.	.	.
cord-277909-rn1dow26	224	1	Positive	positive	JJ
cord-277909-rn1dow26	224	2	and	and	CC
cord-277909-rn1dow26	224	3	negative	negative	JJ
cord-277909-rn1dow26	224	4	controls	control	NNS
cord-277909-rn1dow26	224	5	are	be	VBP
cord-277909-rn1dow26	224	6	an	an	DT
cord-277909-rn1dow26	224	7	essential	essential	JJ
cord-277909-rn1dow26	224	8	part	part	NN
cord-277909-rn1dow26	224	9	of	of	IN
cord-277909-rn1dow26	224	10	any	any	DT
cord-277909-rn1dow26	224	11	diagnostic	diagnostic	JJ
cord-277909-rn1dow26	224	12	PCR	PCR	NNP
cord-277909-rn1dow26	224	13	service	service	NN
cord-277909-rn1dow26	224	14	.	.	.
cord-277909-rn1dow26	225	1	Until	until	IN
cord-277909-rn1dow26	225	2	recently	recently	RB
cord-277909-rn1dow26	225	3	,	,	,
cord-277909-rn1dow26	225	4	we	-PRON-	PRP
cord-277909-rn1dow26	225	5	,	,	,
cord-277909-rn1dow26	225	6	and	and	CC
cord-277909-rn1dow26	225	7	many	many	JJ
cord-277909-rn1dow26	225	8	other	other	JJ
cord-277909-rn1dow26	225	9	laboratories	laboratory	NNS
cord-277909-rn1dow26	225	10	,	,	,
cord-277909-rn1dow26	225	11	utilised	utilise	VBN
cord-277909-rn1dow26	225	12	two	two	CD
cord-277909-rn1dow26	225	13	dilutions	dilution	NNS
cord-277909-rn1dow26	225	14	of	of	IN
cord-277909-rn1dow26	225	15	a	a	DT
cord-277909-rn1dow26	225	16	positive	positive	JJ
cord-277909-rn1dow26	225	17	control	control	NN
cord-277909-rn1dow26	225	18	for	for	IN
cord-277909-rn1dow26	225	19	each	each	DT
cord-277909-rn1dow26	225	20	virus	virus	NN
cord-277909-rn1dow26	225	21	to	to	TO
cord-277909-rn1dow26	225	22	be	be	VB
cord-277909-rn1dow26	225	23	tested	test	VBN
cord-277909-rn1dow26	225	24	(	(	-LRB-
cord-277909-rn1dow26	225	25	the	the	DT
cord-277909-rn1dow26	225	26	end	end	NN
cord-277909-rn1dow26	225	27	-	-	HYPH
cord-277909-rn1dow26	225	28	point	point	NN
cord-277909-rn1dow26	225	29	of	of	IN
cord-277909-rn1dow26	225	30	a	a	DT
cord-277909-rn1dow26	225	31	dilution	dilution	NN
cord-277909-rn1dow26	225	32	series	series	NN
cord-277909-rn1dow26	225	33	of	of	IN
cord-277909-rn1dow26	225	34	cultured	cultured	JJ
cord-277909-rn1dow26	225	35	virus	virus	NN
cord-277909-rn1dow26	225	36	tested	test	VBN
cord-277909-rn1dow26	225	37	in	in	IN
cord-277909-rn1dow26	225	38	the	the	DT
cord-277909-rn1dow26	225	39	relevant	relevant	JJ
cord-277909-rn1dow26	225	40	assay	assay	NN
cord-277909-rn1dow26	225	41	(	(	-LRB-
cord-277909-rn1dow26	225	42	acting	act	VBG
cord-277909-rn1dow26	225	43	as	as	IN
cord-277909-rn1dow26	225	44	a	a	DT
cord-277909-rn1dow26	225	45	sensitivity	sensitivity	NN
cord-277909-rn1dow26	225	46	control	control	NN
cord-277909-rn1dow26	225	47	)	)	-RRB-
cord-277909-rn1dow26	225	48	and	and	CC
cord-277909-rn1dow26	225	49	the	the	DT
cord-277909-rn1dow26	225	50	dilution	dilution	NN
cord-277909-rn1dow26	225	51	1	1	CD
cord-277909-rn1dow26	225	52	log	log	NN
cord-277909-rn1dow26	225	53	less	less	RBR
cord-277909-rn1dow26	225	54	dilute	dilute	NN
cord-277909-rn1dow26	225	55	)	)	-RRB-
cord-277909-rn1dow26	225	56	.	.	.
cord-277909-rn1dow26	226	1	As	as	IN
cord-277909-rn1dow26	226	2	a	a	DT
cord-277909-rn1dow26	226	3	result	result	NN
cord-277909-rn1dow26	226	4	,	,	,
cord-277909-rn1dow26	226	5	for	for	IN
cord-277909-rn1dow26	226	6	each	each	DT
cord-277909-rn1dow26	226	7	robot	robot	NN
cord-277909-rn1dow26	226	8	extraction	extraction	NN
cord-277909-rn1dow26	226	9	run	run	NN
cord-277909-rn1dow26	226	10	of	of	IN
cord-277909-rn1dow26	226	11	96	96	CD
cord-277909-rn1dow26	226	12	wells	well	NNS
cord-277909-rn1dow26	226	13	,	,	,
cord-277909-rn1dow26	226	14	a	a	DT
cord-277909-rn1dow26	226	15	substantial	substantial	JJ
cord-277909-rn1dow26	226	16	number	number	NN
cord-277909-rn1dow26	226	17	of	of	IN
cord-277909-rn1dow26	226	18	wells	well	NNS
cord-277909-rn1dow26	226	19	were	be	VBD
cord-277909-rn1dow26	226	20	required	require	VBN
cord-277909-rn1dow26	226	21	for	for	IN
cord-277909-rn1dow26	226	22	the	the	DT
cord-277909-rn1dow26	226	23	positive	positive	JJ
cord-277909-rn1dow26	226	24	controls	control	NNS
cord-277909-rn1dow26	226	25	alone	alone	RB
cord-277909-rn1dow26	226	26	.	.	.
cord-277909-rn1dow26	227	1	The	the	DT
cord-277909-rn1dow26	227	2	inclusion	inclusion	NN
cord-277909-rn1dow26	227	3	of	of	IN
cord-277909-rn1dow26	227	4	negative	negative	JJ
cord-277909-rn1dow26	227	5	extraction	extraction	NN
cord-277909-rn1dow26	227	6	controls	control	NNS
cord-277909-rn1dow26	227	7	further	further	RB
cord-277909-rn1dow26	227	8	reduces	reduce	VBZ
cord-277909-rn1dow26	227	9	the	the	DT
cord-277909-rn1dow26	227	10	possible	possible	JJ
cord-277909-rn1dow26	227	11	number	number	NN
cord-277909-rn1dow26	227	12	of	of	IN
cord-277909-rn1dow26	227	13	extractions	extraction	NNS
cord-277909-rn1dow26	227	14	available	available	JJ
cord-277909-rn1dow26	227	15	for	for	IN
cord-277909-rn1dow26	227	16	samples	sample	NNS
cord-277909-rn1dow26	227	17	.	.	.
cord-277909-rn1dow26	228	1	The	the	DT
cord-277909-rn1dow26	228	2	use	use	NN
cord-277909-rn1dow26	228	3	of	of	IN
cord-277909-rn1dow26	228	4	numerous	numerous	JJ
cord-277909-rn1dow26	228	5	controls	control	NNS
cord-277909-rn1dow26	228	6	increases	increase	VBZ
cord-277909-rn1dow26	228	7	the	the	DT
cord-277909-rn1dow26	228	8	cost	cost	NN
cord-277909-rn1dow26	228	9	per	per	IN
cord-277909-rn1dow26	228	10	sample	sample	NN
cord-277909-rn1dow26	228	11	and	and	CC
cord-277909-rn1dow26	228	12	the	the	DT
cord-277909-rn1dow26	228	13	turnaround	turnaround	NN
cord-277909-rn1dow26	228	14	times	time	NNS
cord-277909-rn1dow26	228	15	of	of	IN
cord-277909-rn1dow26	228	16	the	the	DT
cord-277909-rn1dow26	228	17	service	service	NN
cord-277909-rn1dow26	228	18	.	.	.
cord-277909-rn1dow26	229	1	Pooled	pool	VBN
cord-277909-rn1dow26	229	2	controls	control	NNS
cord-277909-rn1dow26	229	3	are	be	VBP
cord-277909-rn1dow26	229	4	a	a	DT
cord-277909-rn1dow26	229	5	significant	significant	JJ
cord-277909-rn1dow26	229	6	improvement	improvement	NN
cord-277909-rn1dow26	229	7	on	on	IN
cord-277909-rn1dow26	229	8	the	the	DT
cord-277909-rn1dow26	229	9	previous	previous	JJ
cord-277909-rn1dow26	229	10	method	method	NN
cord-277909-rn1dow26	229	11	and	and	CC
cord-277909-rn1dow26	229	12	we	-PRON-	PRP
cord-277909-rn1dow26	229	13	now	now	RB
cord-277909-rn1dow26	229	14	use	use	VBP
cord-277909-rn1dow26	229	15	separate	separate	JJ
cord-277909-rn1dow26	229	16	pools	pool	NNS
cord-277909-rn1dow26	229	17	containing	contain	VBG
cord-277909-rn1dow26	229	18	12	12	CD
cord-277909-rn1dow26	229	19	respiratory	respiratory	JJ
cord-277909-rn1dow26	229	20	viruses	virus	NNS
cord-277909-rn1dow26	229	21	,	,	,
cord-277909-rn1dow26	229	22	and	and	CC
cord-277909-rn1dow26	229	23	6	6	CD
cord-277909-rn1dow26	229	24	gastrointestinal	gastrointestinal	JJ
cord-277909-rn1dow26	229	25	pathogens	pathogen	NNS
cord-277909-rn1dow26	229	26	.	.	.
cord-277909-rn1dow26	230	1	In	in	IN
cord-277909-rn1dow26	230	2	order	order	NN
cord-277909-rn1dow26	230	3	to	to	TO
cord-277909-rn1dow26	230	4	develop	develop	VB
cord-277909-rn1dow26	230	5	a	a	DT
cord-277909-rn1dow26	230	6	pooled	pool	VBN
cord-277909-rn1dow26	230	7	respiratory	respiratory	JJ
cord-277909-rn1dow26	230	8	or	or	CC
cord-277909-rn1dow26	230	9	gastrointestinal	gastrointestinal	JJ
cord-277909-rn1dow26	230	10	control	control	NN
cord-277909-rn1dow26	230	11	,	,	,
cord-277909-rn1dow26	230	12	each	each	DT
cord-277909-rn1dow26	230	13	virus	virus	NN
cord-277909-rn1dow26	230	14	culture	culture	NN
cord-277909-rn1dow26	230	15	or	or	CC
cord-277909-rn1dow26	230	16	stool	stool	NN
cord-277909-rn1dow26	230	17	extract	extract	NN
cord-277909-rn1dow26	230	18	was	be	VBD
cord-277909-rn1dow26	230	19	serially	serially	RB
cord-277909-rn1dow26	230	20	diluted	dilute	VBN
cord-277909-rn1dow26	230	21	and	and	CC
cord-277909-rn1dow26	230	22	Table	Table	NNP
cord-277909-rn1dow26	230	23	7	7	CD
cord-277909-rn1dow26	230	24	Ct	Ct	NNP
cord-277909-rn1dow26	230	25	of	of	IN
cord-277909-rn1dow26	230	26	positive	positive	JJ
cord-277909-rn1dow26	230	27	control	control	NN
cord-277909-rn1dow26	230	28	on	on	IN
cord-277909-rn1dow26	230	29	frozen	frozen	JJ
cord-277909-rn1dow26	230	30	primer	primer	NN
cord-277909-rn1dow26	230	31	/	/	SYM
cord-277909-rn1dow26	230	32	probe	probe	NN
cord-277909-rn1dow26	230	33	pools	pool	NNS
cord-277909-rn1dow26	230	34	at	at	IN
cord-277909-rn1dow26	230	35	4	4	CD
cord-277909-rn1dow26	230	36	,	,	,
cord-277909-rn1dow26	230	37	6	6	CD
cord-277909-rn1dow26	230	38	and	and	CC
cord-277909-rn1dow26	230	39	8	8	CD
cord-277909-rn1dow26	230	40	weeks	week	NNS
cord-277909-rn1dow26	230	41	an	an	DT
cord-277909-rn1dow26	230	42	end	end	NN
cord-277909-rn1dow26	230	43	point	point	NN
cord-277909-rn1dow26	230	44	established	establish	VBN
cord-277909-rn1dow26	230	45	.	.	.
cord-277909-rn1dow26	231	1	For	for	IN
cord-277909-rn1dow26	231	2	the	the	DT
cord-277909-rn1dow26	231	3	respiratory	respiratory	JJ
cord-277909-rn1dow26	231	4	virus	virus	NN
cord-277909-rn1dow26	231	5	control	control	VBP
cord-277909-rn1dow26	231	6	a	a	DT
cord-277909-rn1dow26	231	7	'	'	``
cord-277909-rn1dow26	231	8	High	high	JJ
cord-277909-rn1dow26	231	9	'	'	''
cord-277909-rn1dow26	231	10	positive	positive	JJ
cord-277909-rn1dow26	231	11	control	control	NN
cord-277909-rn1dow26	231	12	pool	pool	NN
cord-277909-rn1dow26	231	13	was	be	VBD
cord-277909-rn1dow26	231	14	prepared	prepare	VBN
cord-277909-rn1dow26	231	15	by	by	IN
cord-277909-rn1dow26	231	16	adding	add	VBG
cord-277909-rn1dow26	231	17	an	an	DT
cord-277909-rn1dow26	231	18	equal	equal	JJ
cord-277909-rn1dow26	231	19	volume	volume	NN
cord-277909-rn1dow26	231	20	of	of	IN
cord-277909-rn1dow26	231	21	the	the	DT
cord-277909-rn1dow26	231	22	dilution	dilution	NN
cord-277909-rn1dow26	231	23	2	2	CD
cord-277909-rn1dow26	231	24	logs	log	NNS
cord-277909-rn1dow26	231	25	above	above	IN
cord-277909-rn1dow26	231	26	the	the	DT
cord-277909-rn1dow26	231	27	end	end	NN
cord-277909-rn1dow26	231	28	point	point	NN
cord-277909-rn1dow26	231	29	for	for	IN
cord-277909-rn1dow26	231	30	each	each	DT
cord-277909-rn1dow26	231	31	of	of	IN
cord-277909-rn1dow26	231	32	the	the	DT
cord-277909-rn1dow26	231	33	12	12	CD
cord-277909-rn1dow26	231	34	culture	culture	NN
cord-277909-rn1dow26	231	35	fluids	fluid	NNS
cord-277909-rn1dow26	231	36	.	.	.
cord-277909-rn1dow26	232	1	A	a	DT
cord-277909-rn1dow26	232	2	further	further	JJ
cord-277909-rn1dow26	232	3	1	1	CD
cord-277909-rn1dow26	232	4	in	in	IN
cord-277909-rn1dow26	232	5	10	10	CD
cord-277909-rn1dow26	232	6	dilution	dilution	NN
cord-277909-rn1dow26	232	7	of	of	IN
cord-277909-rn1dow26	232	8	this	this	DT
cord-277909-rn1dow26	232	9	'	'	``
cord-277909-rn1dow26	232	10	High	high	JJ
cord-277909-rn1dow26	232	11	'	'	''
cord-277909-rn1dow26	232	12	positive	positive	JJ
cord-277909-rn1dow26	232	13	control	control	NN
cord-277909-rn1dow26	232	14	was	be	VBD
cord-277909-rn1dow26	232	15	prepared	prepared	JJ
cord-277909-rn1dow26	232	16	to	to	TO
cord-277909-rn1dow26	232	17	produce	produce	VB
cord-277909-rn1dow26	232	18	the	the	DT
cord-277909-rn1dow26	232	19	'	'	``
cord-277909-rn1dow26	232	20	Low	low	JJ
cord-277909-rn1dow26	232	21	'	'	''
cord-277909-rn1dow26	232	22	positive	positive	JJ
cord-277909-rn1dow26	232	23	control	control	NN
cord-277909-rn1dow26	232	24	.	.	.
cord-277909-rn1dow26	233	1	We	-PRON-	PRP
cord-277909-rn1dow26	233	2	now	now	RB
cord-277909-rn1dow26	233	3	include	include	VBP
cord-277909-rn1dow26	233	4	just	just	RB
cord-277909-rn1dow26	233	5	two	two	CD
cord-277909-rn1dow26	233	6	respiratory	respiratory	JJ
cord-277909-rn1dow26	233	7	controls	control	NNS
cord-277909-rn1dow26	233	8	on	on	IN
cord-277909-rn1dow26	233	9	our	-PRON-	PRP$
cord-277909-rn1dow26	233	10	robot	robot	NN
cord-277909-rn1dow26	233	11	extractions	extraction	NNS
cord-277909-rn1dow26	233	12	,	,	,
cord-277909-rn1dow26	233	13	freeing	free	VBG
cord-277909-rn1dow26	233	14	up	up	RP
cord-277909-rn1dow26	233	15	an	an	DT
cord-277909-rn1dow26	233	16	additional	additional	JJ
cord-277909-rn1dow26	233	17	22	22	CD
cord-277909-rn1dow26	233	18	wells	well	NNS
cord-277909-rn1dow26	233	19	for	for	IN
cord-277909-rn1dow26	233	20	other	other	JJ
cord-277909-rn1dow26	233	21	samples	sample	NNS
cord-277909-rn1dow26	233	22	.	.	.
cord-277909-rn1dow26	234	1	The	the	DT
cord-277909-rn1dow26	234	2	preparation	preparation	NN
cord-277909-rn1dow26	234	3	of	of	IN
cord-277909-rn1dow26	234	4	a	a	DT
cord-277909-rn1dow26	234	5	large	large	JJ
cord-277909-rn1dow26	234	6	volume	volume	NN
cord-277909-rn1dow26	234	7	of	of	IN
cord-277909-rn1dow26	234	8	control	control	NN
cord-277909-rn1dow26	234	9	at	at	IN
cord-277909-rn1dow26	234	10	the	the	DT
cord-277909-rn1dow26	234	11	one	one	CD
cord-277909-rn1dow26	234	12	time	time	NN
cord-277909-rn1dow26	234	13	allowed	allow	VBN
cord-277909-rn1dow26	234	14	better	well	JJR
cord-277909-rn1dow26	234	15	QC	QC	NNP
cord-277909-rn1dow26	234	16	and	and	CC
cord-277909-rn1dow26	234	17	reproducibility	reproducibility	NN
cord-277909-rn1dow26	234	18	to	to	TO
cord-277909-rn1dow26	234	19	be	be	VB
cord-277909-rn1dow26	234	20	achieved	achieve	VBN
cord-277909-rn1dow26	234	21	.	.	.
cord-277909-rn1dow26	235	1	Aliquots	Aliquots	NNP
cord-277909-rn1dow26	235	2	of	of	IN
cord-277909-rn1dow26	235	3	control	control	NN
cord-277909-rn1dow26	235	4	are	be	VBP
cord-277909-rn1dow26	235	5	stored	store	VBN
cord-277909-rn1dow26	235	6	at	at	IN
cord-277909-rn1dow26	235	7	−70	−70	NNP
cord-277909-rn1dow26	235	8	•	•	NN
cord-277909-rn1dow26	235	9	C	C	NNP
cord-277909-rn1dow26	235	10	and	and	CC
cord-277909-rn1dow26	235	11	have	have	VBP
cord-277909-rn1dow26	235	12	been	be	VBN
cord-277909-rn1dow26	235	13	found	find	VBN
cord-277909-rn1dow26	235	14	to	to	TO
cord-277909-rn1dow26	235	15	be	be	VB
cord-277909-rn1dow26	235	16	stable	stable	JJ
cord-277909-rn1dow26	235	17	for	for	IN
cord-277909-rn1dow26	235	18	up	up	IN
cord-277909-rn1dow26	235	19	to	to	TO
cord-277909-rn1dow26	235	20	3	3	CD
cord-277909-rn1dow26	235	21	months	month	NNS
cord-277909-rn1dow26	235	22	so	so	RB
cord-277909-rn1dow26	235	23	far	far	RB
cord-277909-rn1dow26	235	24	.	.	.
cord-277909-rn1dow26	236	1	We	-PRON-	PRP
cord-277909-rn1dow26	236	2	have	have	VBP
cord-277909-rn1dow26	236	3	previously	previously	RB
cord-277909-rn1dow26	236	4	experienced	experience	VBN
cord-277909-rn1dow26	236	5	lot	lot	NN
cord-277909-rn1dow26	236	6	-	-	HYPH
cord-277909-rn1dow26	236	7	to	to	IN
cord-277909-rn1dow26	236	8	-	-	HYPH
cord-277909-rn1dow26	236	9	lot	lot	NN
cord-277909-rn1dow26	236	10	variation	variation	NN
cord-277909-rn1dow26	236	11	of	of	IN
cord-277909-rn1dow26	236	12	both	both	DT
cord-277909-rn1dow26	236	13	primers	primer	NNS
cord-277909-rn1dow26	236	14	and	and	CC
cord-277909-rn1dow26	236	15	probes	probe	NNS
cord-277909-rn1dow26	236	16	resulting	result	VBG
cord-277909-rn1dow26	236	17	in	in	IN
cord-277909-rn1dow26	236	18	reductions	reduction	NNS
cord-277909-rn1dow26	236	19	in	in	IN
cord-277909-rn1dow26	236	20	test	test	NN
cord-277909-rn1dow26	236	21	sensitivity	sensitivity	NN
cord-277909-rn1dow26	236	22	.	.	.
cord-277909-rn1dow26	237	1	When	when	WRB
cord-277909-rn1dow26	237	2	a	a	DT
cord-277909-rn1dow26	237	3	new	new	JJ
cord-277909-rn1dow26	237	4	batch	batch	NN
cord-277909-rn1dow26	237	5	of	of	IN
cord-277909-rn1dow26	237	6	reagents	reagent	NNS
cord-277909-rn1dow26	237	7	is	be	VBZ
cord-277909-rn1dow26	237	8	purchased	purchase	VBN
cord-277909-rn1dow26	237	9	we	-PRON-	PRP
cord-277909-rn1dow26	237	10	now	now	RB
cord-277909-rn1dow26	237	11	run	run	VBP
cord-277909-rn1dow26	237	12	a	a	DT
cord-277909-rn1dow26	237	13	performance	performance	NN
cord-277909-rn1dow26	237	14	test	test	NN
cord-277909-rn1dow26	237	15	(	(	-LRB-
cord-277909-rn1dow26	237	16	using	use	VBG
cord-277909-rn1dow26	237	17	the	the	DT
cord-277909-rn1dow26	237	18	new	new	JJ
cord-277909-rn1dow26	237	19	reagents	reagent	NNS
cord-277909-rn1dow26	237	20	at	at	IN
cord-277909-rn1dow26	237	21	the	the	DT
cord-277909-rn1dow26	237	22	same	same	JJ
cord-277909-rn1dow26	237	23	concentrations	concentration	NNS
cord-277909-rn1dow26	237	24	previously	previously	RB
cord-277909-rn1dow26	237	25	determined	determine	VBN
cord-277909-rn1dow26	237	26	as	as	IN
cord-277909-rn1dow26	237	27	optimal	optimal	JJ
cord-277909-rn1dow26	237	28	)	)	-RRB-
cord-277909-rn1dow26	237	29	by	by	IN
cord-277909-rn1dow26	237	30	testing	test	VBG
cord-277909-rn1dow26	237	31	the	the	DT
cord-277909-rn1dow26	237	32	'	'	``
cord-277909-rn1dow26	237	33	old	old	JJ
cord-277909-rn1dow26	237	34	'	'	''
cord-277909-rn1dow26	237	35	and	and	CC
cord-277909-rn1dow26	237	36	'	'	``
cord-277909-rn1dow26	237	37	new	new	JJ
cord-277909-rn1dow26	237	38	'	'	''
cord-277909-rn1dow26	237	39	primer	primer	NN
cord-277909-rn1dow26	237	40	probe	probe	NN
cord-277909-rn1dow26	237	41	sets	set	NNS
cord-277909-rn1dow26	237	42	in	in	IN
cord-277909-rn1dow26	237	43	parallel	parallel	NN
cord-277909-rn1dow26	237	44	with	with	IN
cord-277909-rn1dow26	237	45	the	the	DT
cord-277909-rn1dow26	237	46	same	same	JJ
cord-277909-rn1dow26	237	47	positive	positive	JJ
cord-277909-rn1dow26	237	48	control	control	NN
cord-277909-rn1dow26	237	49	on	on	IN
cord-277909-rn1dow26	237	50	the	the	DT
cord-277909-rn1dow26	237	51	same	same	JJ
cord-277909-rn1dow26	237	52	PCR	PCR	NNP
cord-277909-rn1dow26	237	53	run	run	NN
cord-277909-rn1dow26	237	54	.	.	.
cord-277909-rn1dow26	238	1	If	if	IN
cord-277909-rn1dow26	238	2	the	the	DT
cord-277909-rn1dow26	238	3	C	C	NNP
cord-277909-rn1dow26	238	4	T	T	NNP
cord-277909-rn1dow26	238	5	and	and	CC
cord-277909-rn1dow26	238	6	R	r	NN
cord-277909-rn1dow26	238	7	n	n	CD
cord-277909-rn1dow26	238	8	values	value	NNS
cord-277909-rn1dow26	238	9	observed	observe	VBN
cord-277909-rn1dow26	238	10	are	be	VBP
cord-277909-rn1dow26	238	11	comparable	comparable	JJ
cord-277909-rn1dow26	238	12	(	(	-LRB-
cord-277909-rn1dow26	238	13	newly	newly	RB
cord-277909-rn1dow26	238	14	prepared	prepare	VBN
cord-277909-rn1dow26	238	15	reagents	reagent	NNS
cord-277909-rn1dow26	238	16	must	must	MD
cord-277909-rn1dow26	238	17	produce	produce	VB
cord-277909-rn1dow26	238	18	C	C	NNP
cord-277909-rn1dow26	238	19	T	t	NN
cord-277909-rn1dow26	238	20	values	value	NNS
cord-277909-rn1dow26	238	21	falling	fall	VBG
cord-277909-rn1dow26	238	22	within	within	IN
cord-277909-rn1dow26	238	23	two	two	CD
cord-277909-rn1dow26	238	24	standard	standard	JJ
cord-277909-rn1dow26	238	25	deviations	deviation	NNS
cord-277909-rn1dow26	238	26	of	of	IN
cord-277909-rn1dow26	238	27	the	the	DT
cord-277909-rn1dow26	238	28	mean	mean	JJ
cord-277909-rn1dow26	238	29	value	value	NN
cord-277909-rn1dow26	238	30	determined	determine	VBN
cord-277909-rn1dow26	238	31	for	for	IN
cord-277909-rn1dow26	238	32	the	the	DT
cord-277909-rn1dow26	238	33	reagents	reagent	NNS
cord-277909-rn1dow26	238	34	previously	previously	RB
cord-277909-rn1dow26	238	35	in	in	IN
cord-277909-rn1dow26	238	36	use	use	NN
cord-277909-rn1dow26	238	37	(	(	-LRB-
cord-277909-rn1dow26	238	38	when	when	WRB
cord-277909-rn1dow26	238	39	testing	test	VBG
cord-277909-rn1dow26	238	40	identical	identical	JJ
cord-277909-rn1dow26	238	41	positive	positive	JJ
cord-277909-rn1dow26	238	42	controls	control	NNS
cord-277909-rn1dow26	238	43	)	)	-RRB-
cord-277909-rn1dow26	238	44	,	,	,
cord-277909-rn1dow26	238	45	the	the	DT
cord-277909-rn1dow26	238	46	new	new	JJ
cord-277909-rn1dow26	238	47	reagents	reagent	NNS
cord-277909-rn1dow26	238	48	are	be	VBP
cord-277909-rn1dow26	238	49	released	release	VBN
cord-277909-rn1dow26	238	50	for	for	IN
cord-277909-rn1dow26	238	51	routine	routine	JJ
cord-277909-rn1dow26	238	52	use	use	NN
cord-277909-rn1dow26	238	53	.	.	.
cord-277909-rn1dow26	239	1	If	if	IN
cord-277909-rn1dow26	239	2	the	the	DT
cord-277909-rn1dow26	239	3	assay	assay	NN
cord-277909-rn1dow26	239	4	is	be	VBZ
cord-277909-rn1dow26	239	5	less	less	RBR
cord-277909-rn1dow26	239	6	sensitive	sensitive	JJ
cord-277909-rn1dow26	239	7	than	than	IN
cord-277909-rn1dow26	239	8	the	the	DT
cord-277909-rn1dow26	239	9	previous	previous	JJ
cord-277909-rn1dow26	239	10	assay	assay	NN
cord-277909-rn1dow26	239	11	then	then	RB
cord-277909-rn1dow26	239	12	primer	primer	VB
cord-277909-rn1dow26	239	13	and/or	and/or	CC
cord-277909-rn1dow26	239	14	probe	probe	NN
cord-277909-rn1dow26	239	15	optimisation	optimisation	NN
cord-277909-rn1dow26	239	16	should	should	MD
cord-277909-rn1dow26	239	17	take	take	VB
cord-277909-rn1dow26	239	18	place	place	NN
cord-277909-rn1dow26	239	19	.	.	.
cord-277909-rn1dow26	240	1	Ideally	ideally	RB
cord-277909-rn1dow26	240	2	this	this	DT
cord-277909-rn1dow26	240	3	should	should	MD
cord-277909-rn1dow26	240	4	be	be	VB
cord-277909-rn1dow26	240	5	done	do	VBN
cord-277909-rn1dow26	240	6	several	several	JJ
cord-277909-rn1dow26	240	7	weeks	week	NNS
cord-277909-rn1dow26	240	8	before	before	IN
cord-277909-rn1dow26	240	9	the	the	DT
cord-277909-rn1dow26	240	10	next	next	JJ
cord-277909-rn1dow26	240	11	batch	batch	NN
cord-277909-rn1dow26	240	12	is	be	VBZ
cord-277909-rn1dow26	240	13	required	require	VBN
cord-277909-rn1dow26	240	14	for	for	IN
cord-277909-rn1dow26	240	15	routine	routine	JJ
cord-277909-rn1dow26	240	16	use	use	NN
cord-277909-rn1dow26	240	17	as	as	IN
cord-277909-rn1dow26	240	18	new	new	JJ
cord-277909-rn1dow26	240	19	probes	probe	NNS
cord-277909-rn1dow26	240	20	or	or	CC
cord-277909-rn1dow26	240	21	primers	primer	NNS
cord-277909-rn1dow26	240	22	may	may	MD
cord-277909-rn1dow26	240	23	need	need	VB
cord-277909-rn1dow26	240	24	to	to	TO
cord-277909-rn1dow26	240	25	be	be	VB
cord-277909-rn1dow26	240	26	re	re	VBN
cord-277909-rn1dow26	240	27	-	-	VBN
cord-277909-rn1dow26	240	28	ordered	order	VBN
cord-277909-rn1dow26	240	29	.	.	.
cord-277909-rn1dow26	241	1	Our	-PRON-	PRP$
cord-277909-rn1dow26	241	2	experience	experience	NN
cord-277909-rn1dow26	241	3	over	over	IN
cord-277909-rn1dow26	241	4	the	the	DT
cord-277909-rn1dow26	241	5	winter	winter	NN
cord-277909-rn1dow26	241	6	of	of	IN
cord-277909-rn1dow26	241	7	2004	2004	CD
cord-277909-rn1dow26	241	8	-	-	SYM
cord-277909-rn1dow26	241	9	2005	2005	CD
cord-277909-rn1dow26	241	10	is	be	VBZ
cord-277909-rn1dow26	241	11	that	that	IN
cord-277909-rn1dow26	241	12	re	re	NN
cord-277909-rn1dow26	241	13	-	-	NN
cord-277909-rn1dow26	241	14	optimisation	optimisation	NN
cord-277909-rn1dow26	241	15	has	have	VBZ
cord-277909-rn1dow26	241	16	not	not	RB
cord-277909-rn1dow26	241	17	been	be	VBN
cord-277909-rn1dow26	241	18	required	require	VBN
cord-277909-rn1dow26	241	19	for	for	IN
cord-277909-rn1dow26	241	20	any	any	DT
cord-277909-rn1dow26	241	21	of	of	IN
cord-277909-rn1dow26	241	22	the	the	DT
cord-277909-rn1dow26	241	23	respiratory	respiratory	JJ
cord-277909-rn1dow26	241	24	assays	assay	NNS
cord-277909-rn1dow26	241	25	.	.	.
cord-277909-rn1dow26	242	1	For	for	IN
cord-277909-rn1dow26	242	2	validation	validation	NN
cord-277909-rn1dow26	242	3	of	of	IN
cord-277909-rn1dow26	242	4	each	each	DT
cord-277909-rn1dow26	242	5	real	real	JJ
cord-277909-rn1dow26	242	6	time	time	NN
cord-277909-rn1dow26	242	7	PCR	PCR	NNP
cord-277909-rn1dow26	242	8	run	run	VBN
cord-277909-rn1dow26	242	9	we	-PRON-	PRP
cord-277909-rn1dow26	242	10	recommend	recommend	VBP
cord-277909-rn1dow26	242	11	the	the	DT
cord-277909-rn1dow26	242	12	following	following	NN
cord-277909-rn1dow26	242	13	.	.	.
cord-277909-rn1dow26	243	1	The	the	DT
cord-277909-rn1dow26	243	2	C	C	NNP
cord-277909-rn1dow26	243	3	T	t	NN
cord-277909-rn1dow26	243	4	of	of	IN
cord-277909-rn1dow26	243	5	the	the	DT
cord-277909-rn1dow26	243	6	positive	positive	JJ
cord-277909-rn1dow26	243	7	control	control	NN
cord-277909-rn1dow26	243	8	should	should	MD
cord-277909-rn1dow26	243	9	be	be	VB
cord-277909-rn1dow26	243	10	documented	document	VBN
cord-277909-rn1dow26	243	11	with	with	IN
cord-277909-rn1dow26	243	12	each	each	DT
cord-277909-rn1dow26	243	13	run	run	NN
cord-277909-rn1dow26	243	14	and	and	CC
cord-277909-rn1dow26	243	15	compared	compare	VBN
cord-277909-rn1dow26	243	16	to	to	IN
cord-277909-rn1dow26	243	17	the	the	DT
cord-277909-rn1dow26	243	18	value	value	NN
cord-277909-rn1dow26	243	19	derived	derive	VBN
cord-277909-rn1dow26	243	20	from	from	IN
cord-277909-rn1dow26	243	21	previous	previous	JJ
cord-277909-rn1dow26	243	22	runs	run	NNS
cord-277909-rn1dow26	243	23	.	.	.
cord-277909-rn1dow26	244	1	This	this	DT
cord-277909-rn1dow26	244	2	should	should	MD
cord-277909-rn1dow26	244	3	help	help	VB
cord-277909-rn1dow26	244	4	identify	identify	VB
cord-277909-rn1dow26	244	5	any	any	DT
cord-277909-rn1dow26	244	6	loss	loss	NN
cord-277909-rn1dow26	244	7	in	in	IN
cord-277909-rn1dow26	244	8	sensitivity	sensitivity	NN
cord-277909-rn1dow26	244	9	that	that	WDT
cord-277909-rn1dow26	244	10	can	can	MD
cord-277909-rn1dow26	244	11	be	be	VB
cord-277909-rn1dow26	244	12	seen	see	VBN
cord-277909-rn1dow26	244	13	due	due	IN
cord-277909-rn1dow26	244	14	to	to	IN
cord-277909-rn1dow26	244	15	user	user	NN
cord-277909-rn1dow26	244	16	error	error	NN
cord-277909-rn1dow26	244	17	or	or	CC
cord-277909-rn1dow26	244	18	degradation	degradation	NN
cord-277909-rn1dow26	244	19	of	of	IN
cord-277909-rn1dow26	244	20	PCR	PCR	NNP
cord-277909-rn1dow26	244	21	reagents	reagent	NNS
cord-277909-rn1dow26	244	22	.	.	.
cord-277909-rn1dow26	245	1	If	if	IN
cord-277909-rn1dow26	245	2	the	the	DT
cord-277909-rn1dow26	245	3	C	c	NN
cord-277909-rn1dow26	245	4	T	T	NNP
cord-277909-rn1dow26	245	5	falls	fall	VBZ
cord-277909-rn1dow26	245	6	significantly	significantly	RB
cord-277909-rn1dow26	245	7	below	below	IN
cord-277909-rn1dow26	245	8	the	the	DT
cord-277909-rn1dow26	245	9	expected	expect	VBN
cord-277909-rn1dow26	245	10	value	value	NN
cord-277909-rn1dow26	245	11	the	the	DT
cord-277909-rn1dow26	245	12	run	run	NN
cord-277909-rn1dow26	245	13	should	should	MD
cord-277909-rn1dow26	245	14	be	be	VB
cord-277909-rn1dow26	245	15	repeated	repeat	VBN
cord-277909-rn1dow26	245	16	(	(	-LRB-
cord-277909-rn1dow26	245	17	outwith	outwith	IN
cord-277909-rn1dow26	245	18	two	two	CD
cord-277909-rn1dow26	245	19	standard	standard	JJ
cord-277909-rn1dow26	245	20	deviations	deviation	NNS
cord-277909-rn1dow26	245	21	of	of	IN
cord-277909-rn1dow26	245	22	the	the	DT
cord-277909-rn1dow26	245	23	mean	mean	JJ
cord-277909-rn1dow26	245	24	value	value	NN
cord-277909-rn1dow26	245	25	determined	determine	VBN
cord-277909-rn1dow26	245	26	by	by	IN
cord-277909-rn1dow26	245	27	previous	previous	JJ
cord-277909-rn1dow26	245	28	runs	run	NNS
cord-277909-rn1dow26	245	29	(	(	-LRB-
cord-277909-rn1dow26	245	30	when	when	WRB
cord-277909-rn1dow26	245	31	testing	test	VBG
cord-277909-rn1dow26	245	32	identical	identical	JJ
cord-277909-rn1dow26	245	33	positive	positive	JJ
cord-277909-rn1dow26	245	34	controls	control	NNS
cord-277909-rn1dow26	245	35	)	)	-RRB-
cord-277909-rn1dow26	245	36	.	.	.
cord-277909-rn1dow26	246	1	If	if	IN
cord-277909-rn1dow26	246	2	the	the	DT
cord-277909-rn1dow26	246	3	C	c	NN
cord-277909-rn1dow26	246	4	T	t	NN
cord-277909-rn1dow26	246	5	remains	remain	VBZ
cord-277909-rn1dow26	246	6	low	low	JJ
cord-277909-rn1dow26	246	7	or	or	CC
cord-277909-rn1dow26	246	8	reduces	reduce	VBZ
cord-277909-rn1dow26	246	9	further	further	RB
cord-277909-rn1dow26	246	10	,	,	,
cord-277909-rn1dow26	246	11	new	new	JJ
cord-277909-rn1dow26	246	12	controls	control	NNS
cord-277909-rn1dow26	246	13	and	and	CC
cord-277909-rn1dow26	246	14	PCR	PCR	NNP
cord-277909-rn1dow26	246	15	reagents	reagent	NNS
cord-277909-rn1dow26	246	16	may	may	MD
cord-277909-rn1dow26	246	17	be	be	VB
cord-277909-rn1dow26	246	18	required	require	VBN
cord-277909-rn1dow26	246	19	.	.	.
cord-277909-rn1dow26	247	1	In	in	IN
cord-277909-rn1dow26	247	2	addition	addition	NN
cord-277909-rn1dow26	247	3	to	to	IN
cord-277909-rn1dow26	247	4	this	this	DT
cord-277909-rn1dow26	247	5	the	the	DT
cord-277909-rn1dow26	247	6	overall	overall	JJ
cord-277909-rn1dow26	247	7	fluorescence	fluorescence	NN
cord-277909-rn1dow26	247	8	change	change	NN
cord-277909-rn1dow26	247	9	should	should	MD
cord-277909-rn1dow26	247	10	also	also	RB
cord-277909-rn1dow26	247	11	be	be	VB
cord-277909-rn1dow26	247	12	monitored	monitor	VBN
cord-277909-rn1dow26	247	13	with	with	IN
cord-277909-rn1dow26	247	14	each	each	DT
cord-277909-rn1dow26	247	15	run	run	NN
cord-277909-rn1dow26	247	16	.	.	.
cord-277909-rn1dow26	248	1	Reductions	reduction	NNS
cord-277909-rn1dow26	248	2	in	in	IN
cord-277909-rn1dow26	248	3	fluorescence	fluorescence	NN
cord-277909-rn1dow26	248	4	may	may	MD
cord-277909-rn1dow26	248	5	cause	cause	VB
cord-277909-rn1dow26	248	6	interpretation	interpretation	NN
cord-277909-rn1dow26	248	7	difficulties	difficulty	NNS
cord-277909-rn1dow26	248	8	and	and	CC
cord-277909-rn1dow26	248	9	may	may	MD
cord-277909-rn1dow26	248	10	also	also	RB
cord-277909-rn1dow26	248	11	highlight	highlight	VB
cord-277909-rn1dow26	248	12	a	a	DT
cord-277909-rn1dow26	248	13	problem	problem	NN
cord-277909-rn1dow26	248	14	in	in	IN
cord-277909-rn1dow26	248	15	the	the	DT
cord-277909-rn1dow26	248	16	PCR	PCR	NNP
cord-277909-rn1dow26	248	17	reaction	reaction	NN
cord-277909-rn1dow26	248	18	.	.	.
cord-277909-rn1dow26	249	1	As	as	IN
cord-277909-rn1dow26	249	2	with	with	IN
cord-277909-rn1dow26	249	3	changes	change	NNS
cord-277909-rn1dow26	249	4	in	in	IN
cord-277909-rn1dow26	249	5	C	C	NNP
cord-277909-rn1dow26	249	6	T	T	NNP
cord-277909-rn1dow26	249	7	,	,	,
cord-277909-rn1dow26	249	8	large	large	JJ
cord-277909-rn1dow26	249	9	reductions	reduction	NNS
cord-277909-rn1dow26	249	10	in	in	IN
cord-277909-rn1dow26	249	11	fluorescence	fluorescence	NN
cord-277909-rn1dow26	249	12	may	may	MD
cord-277909-rn1dow26	249	13	result	result	VB
cord-277909-rn1dow26	249	14	in	in	IN
cord-277909-rn1dow26	249	15	the	the	DT
cord-277909-rn1dow26	249	16	need	need	NN
cord-277909-rn1dow26	249	17	to	to	TO
cord-277909-rn1dow26	249	18	repeat	repeat	VB
cord-277909-rn1dow26	249	19	the	the	DT
cord-277909-rn1dow26	249	20	PCR	PCR	NNP
cord-277909-rn1dow26	249	21	or	or	CC
cord-277909-rn1dow26	249	22	introduce	introduce	VB
cord-277909-rn1dow26	249	23	a	a	DT
cord-277909-rn1dow26	249	24	new	new	JJ
cord-277909-rn1dow26	249	25	batch	batch	NN
cord-277909-rn1dow26	249	26	of	of	IN
cord-277909-rn1dow26	249	27	controls	control	NNS
cord-277909-rn1dow26	249	28	and	and	CC
cord-277909-rn1dow26	249	29	PCR	PCR	NNP
cord-277909-rn1dow26	249	30	reagents	reagent	NNS
cord-277909-rn1dow26	249	31	.	.	.
cord-277909-rn1dow26	250	1	Ideally	ideally	RB
cord-277909-rn1dow26	250	2	real	real	JJ
cord-277909-rn1dow26	250	3	time	time	NN
cord-277909-rn1dow26	250	4	PCR	PCR	NNP
cord-277909-rn1dow26	250	5	tests	test	NNS
cord-277909-rn1dow26	250	6	should	should	MD
cord-277909-rn1dow26	250	7	include	include	VB
cord-277909-rn1dow26	250	8	an	an	DT
cord-277909-rn1dow26	250	9	internal	internal	JJ
cord-277909-rn1dow26	250	10	control	control	NN
cord-277909-rn1dow26	250	11	in	in	IN
cord-277909-rn1dow26	250	12	order	order	NN
cord-277909-rn1dow26	250	13	to	to	TO
cord-277909-rn1dow26	250	14	ensure	ensure	VB
cord-277909-rn1dow26	250	15	confidence	confidence	NN
cord-277909-rn1dow26	250	16	in	in	IN
cord-277909-rn1dow26	250	17	negative	negative	JJ
cord-277909-rn1dow26	250	18	results	result	NNS
cord-277909-rn1dow26	250	19	.	.	.
cord-277909-rn1dow26	251	1	There	there	EX
cord-277909-rn1dow26	251	2	are	be	VBP
cord-277909-rn1dow26	251	3	many	many	JJ
cord-277909-rn1dow26	251	4	internal	internal	JJ
cord-277909-rn1dow26	251	5	control	control	NN
cord-277909-rn1dow26	251	6	PCR	PCR	NNP
cord-277909-rn1dow26	251	7	tests	test	NNS
cord-277909-rn1dow26	251	8	available	available	JJ
cord-277909-rn1dow26	251	9	targeting	target	VBG
cord-277909-rn1dow26	251	10	animal	animal	NN
cord-277909-rn1dow26	251	11	viruses	virus	NNS
cord-277909-rn1dow26	251	12	(	(	-LRB-
cord-277909-rn1dow26	251	13	added	add	VBN
cord-277909-rn1dow26	251	14	to	to	IN
cord-277909-rn1dow26	251	15	the	the	DT
cord-277909-rn1dow26	251	16	sample	sample	NN
cord-277909-rn1dow26	251	17	before	before	IN
cord-277909-rn1dow26	251	18	extraction	extraction	NN
cord-277909-rn1dow26	251	19	)	)	-RRB-
cord-277909-rn1dow26	251	20	and	and	CC
cord-277909-rn1dow26	251	21	synthetic	synthetic	JJ
cord-277909-rn1dow26	251	22	controls	control	NNS
cord-277909-rn1dow26	251	23	(	(	-LRB-
cord-277909-rn1dow26	251	24	which	which	WDT
cord-277909-rn1dow26	251	25	are	be	VBP
cord-277909-rn1dow26	251	26	added	add	VBN
cord-277909-rn1dow26	251	27	to	to	IN
cord-277909-rn1dow26	251	28	the	the	DT
cord-277909-rn1dow26	251	29	mastremix	mastremix	NNP
cord-277909-rn1dow26	251	30	)	)	-RRB-
cord-277909-rn1dow26	251	31	,	,	,
cord-277909-rn1dow26	251	32	and	and	CC
cord-277909-rn1dow26	251	33	human	human	JJ
cord-277909-rn1dow26	251	34	genes	gene	NNS
cord-277909-rn1dow26	251	35	.	.	.
cord-277909-rn1dow26	252	1	However	however	RB
cord-277909-rn1dow26	252	2	,	,	,
cord-277909-rn1dow26	252	3	the	the	DT
cord-277909-rn1dow26	252	4	inclusion	inclusion	NN
cord-277909-rn1dow26	252	5	of	of	IN
cord-277909-rn1dow26	252	6	such	such	JJ
cord-277909-rn1dow26	252	7	controls	control	NNS
cord-277909-rn1dow26	252	8	can	can	MD
cord-277909-rn1dow26	252	9	be	be	VB
cord-277909-rn1dow26	252	10	expensive	expensive	JJ
cord-277909-rn1dow26	252	11	as	as	IN
cord-277909-rn1dow26	252	12	they	-PRON-	PRP
cord-277909-rn1dow26	252	13	may	may	MD
cord-277909-rn1dow26	252	14	have	have	VB
cord-277909-rn1dow26	252	15	to	to	TO
cord-277909-rn1dow26	252	16	be	be	VB
cord-277909-rn1dow26	252	17	carried	carry	VBN
cord-277909-rn1dow26	252	18	out	out	RP
cord-277909-rn1dow26	252	19	separately	separately	RB
cord-277909-rn1dow26	252	20	from	from	IN
cord-277909-rn1dow26	252	21	the	the	DT
cord-277909-rn1dow26	252	22	diagnostic	diagnostic	JJ
cord-277909-rn1dow26	252	23	assay	assay	NN
cord-277909-rn1dow26	252	24	.	.	.
cord-277909-rn1dow26	253	1	As	as	IN
cord-277909-rn1dow26	253	2	a	a	DT
cord-277909-rn1dow26	253	3	result	result	NN
cord-277909-rn1dow26	253	4	many	many	JJ
cord-277909-rn1dow26	253	5	laboratories	laboratory	NNS
cord-277909-rn1dow26	253	6	(	(	-LRB-
cord-277909-rn1dow26	253	7	including	include	VBG
cord-277909-rn1dow26	253	8	ourselves	-PRON-	PRP
cord-277909-rn1dow26	253	9	)	)	-RRB-
cord-277909-rn1dow26	253	10	do	do	VBP
cord-277909-rn1dow26	253	11	not	not	RB
cord-277909-rn1dow26	253	12	use	use	VB
cord-277909-rn1dow26	253	13	such	such	JJ
cord-277909-rn1dow26	253	14	controls	control	NNS
cord-277909-rn1dow26	253	15	on	on	IN
cord-277909-rn1dow26	253	16	all	all	DT
cord-277909-rn1dow26	253	17	tests	test	NNS
cord-277909-rn1dow26	253	18	.	.	.
cord-277909-rn1dow26	254	1	Any	any	DT
cord-277909-rn1dow26	254	2	laboratories	laboratory	NNS
cord-277909-rn1dow26	254	3	performing	perform	VBG
cord-277909-rn1dow26	254	4	real	real	JJ
cord-277909-rn1dow26	254	5	time	time	NN
cord-277909-rn1dow26	254	6	PCR	pcr	NN
cord-277909-rn1dow26	254	7	assays	assay	NNS
cord-277909-rn1dow26	254	8	can	can	MD
cord-277909-rn1dow26	254	9	perform	perform	VB
cord-277909-rn1dow26	254	10	quantitative	quantitative	JJ
cord-277909-rn1dow26	254	11	assays	assay	NNS
cord-277909-rn1dow26	254	12	with	with	IN
cord-277909-rn1dow26	254	13	the	the	DT
cord-277909-rn1dow26	254	14	addition	addition	NN
cord-277909-rn1dow26	254	15	of	of	IN
cord-277909-rn1dow26	254	16	suitable	suitable	JJ
cord-277909-rn1dow26	254	17	standard	standard	JJ
cord-277909-rn1dow26	254	18	quantitative	quantitative	JJ
cord-277909-rn1dow26	254	19	controls	control	NNS
cord-277909-rn1dow26	254	20	to	to	IN
cord-277909-rn1dow26	254	21	the	the	DT
cord-277909-rn1dow26	254	22	assay	assay	NN
cord-277909-rn1dow26	254	23	,	,	,
cord-277909-rn1dow26	254	24	although	although	IN
cord-277909-rn1dow26	254	25	a	a	DT
cord-277909-rn1dow26	254	26	uniform	uniform	JJ
cord-277909-rn1dow26	254	27	sample	sample	NN
cord-277909-rn1dow26	254	28	type	type	NN
cord-277909-rn1dow26	254	29	is	be	VBZ
cord-277909-rn1dow26	254	30	required	require	VBN
cord-277909-rn1dow26	254	31	to	to	TO
cord-277909-rn1dow26	254	32	obtain	obtain	VB
cord-277909-rn1dow26	254	33	meaningful	meaningful	JJ
cord-277909-rn1dow26	254	34	results	result	NNS
cord-277909-rn1dow26	254	35	(	(	-LRB-
cord-277909-rn1dow26	254	36	e.g.	e.g.	RB
cord-277909-rn1dow26	254	37	,	,	,
cord-277909-rn1dow26	254	38	blood	blood	NN
cord-277909-rn1dow26	254	39	,	,	,
cord-277909-rn1dow26	254	40	urine	urine	NN
cord-277909-rn1dow26	254	41	)	)	-RRB-
cord-277909-rn1dow26	254	42	.	.	.
cord-277909-rn1dow26	255	1	Attempting	attempt	VBG
cord-277909-rn1dow26	255	2	to	to	TO
cord-277909-rn1dow26	255	3	quantify	quantify	VB
cord-277909-rn1dow26	255	4	virus	virus	NN
cord-277909-rn1dow26	255	5	in	in	IN
cord-277909-rn1dow26	255	6	nonuniform	nonuniform	JJ
cord-277909-rn1dow26	255	7	sample	sample	NN
cord-277909-rn1dow26	255	8	types	type	NNS
cord-277909-rn1dow26	255	9	(	(	-LRB-
cord-277909-rn1dow26	255	10	such	such	JJ
cord-277909-rn1dow26	255	11	as	as	IN
cord-277909-rn1dow26	255	12	respiratory	respiratory	JJ
cord-277909-rn1dow26	255	13	samples	sample	NNS
cord-277909-rn1dow26	255	14	or	or	CC
cord-277909-rn1dow26	255	15	stool	stool	NN
cord-277909-rn1dow26	255	16	)	)	-RRB-
cord-277909-rn1dow26	255	17	is	be	VBZ
cord-277909-rn1dow26	255	18	not	not	RB
cord-277909-rn1dow26	255	19	recommended	recommend	VBN
cord-277909-rn1dow26	255	20	without	without	IN
cord-277909-rn1dow26	255	21	thorough	thorough	JJ
cord-277909-rn1dow26	255	22	assessment	assessment	NN
cord-277909-rn1dow26	255	23	of	of	IN
cord-277909-rn1dow26	255	24	sampling	sampling	NN
cord-277909-rn1dow26	255	25	reproducibility	reproducibility	NN
cord-277909-rn1dow26	255	26	.	.	.
cord-277909-rn1dow26	256	1	In	in	IN
cord-277909-rn1dow26	256	2	common	common	JJ
cord-277909-rn1dow26	256	3	with	with	IN
cord-277909-rn1dow26	256	4	many	many	JJ
cord-277909-rn1dow26	256	5	laboratories	laboratory	NNS
cord-277909-rn1dow26	256	6	,	,	,
cord-277909-rn1dow26	256	7	we	-PRON-	PRP
cord-277909-rn1dow26	256	8	prepare	prepare	VBP
cord-277909-rn1dow26	256	9	our	-PRON-	PRP$
cord-277909-rn1dow26	256	10	quantitative	quantitative	JJ
cord-277909-rn1dow26	256	11	standards	standard	NNS
cord-277909-rn1dow26	256	12	(	(	-LRB-
cord-277909-rn1dow26	256	13	oligos	oligo	NNS
cord-277909-rn1dow26	256	14	or	or	CC
cord-277909-rn1dow26	256	15	plasmids	plasmid	NNS
cord-277909-rn1dow26	256	16	)	)	-RRB-
cord-277909-rn1dow26	256	17	in	in	IN
cord-277909-rn1dow26	256	18	bulk	bulk	NN
cord-277909-rn1dow26	256	19	,	,	,
cord-277909-rn1dow26	256	20	test	test	VB
cord-277909-rn1dow26	256	21	these	these	DT
cord-277909-rn1dow26	256	22	for	for	IN
cord-277909-rn1dow26	256	23	acceptable	acceptable	JJ
cord-277909-rn1dow26	256	24	linearity	linearity	NN
cord-277909-rn1dow26	256	25	and	and	CC
cord-277909-rn1dow26	256	26	slope	slope	NN
cord-277909-rn1dow26	256	27	(	(	-LRB-
cord-277909-rn1dow26	256	28	−3.33	−3.33	NNP
cord-277909-rn1dow26	256	29	)	)	-RRB-
cord-277909-rn1dow26	256	30	for	for	IN
cord-277909-rn1dow26	256	31	a	a	DT
cord-277909-rn1dow26	256	32	good	good	JJ
cord-277909-rn1dow26	256	33	10-fold	10-fold	CD
cord-277909-rn1dow26	256	34	dilution	dilution	NN
cord-277909-rn1dow26	256	35	series	series	NN
cord-277909-rn1dow26	256	36	(	(	-LRB-
cord-277909-rn1dow26	256	37	we	-PRON-	PRP
cord-277909-rn1dow26	256	38	allow	allow	VBP
cord-277909-rn1dow26	256	39	a	a	DT
cord-277909-rn1dow26	256	40	range	range	NN
cord-277909-rn1dow26	256	41	of	of	IN
cord-277909-rn1dow26	256	42	3.18	3.18	CD
cord-277909-rn1dow26	256	43	-	-	SYM
cord-277909-rn1dow26	256	44	3.45	3.45	CD
cord-277909-rn1dow26	256	45	which	which	WDT
cord-277909-rn1dow26	256	46	equates	equate	VBZ
cord-277909-rn1dow26	256	47	to	to	IN
cord-277909-rn1dow26	256	48	a	a	DT
cord-277909-rn1dow26	256	49	variation	variation	NN
cord-277909-rn1dow26	256	50	of	of	IN
cord-277909-rn1dow26	256	51	±5	±5	CD
cord-277909-rn1dow26	256	52	%	%	NN
cord-277909-rn1dow26	256	53	in	in	IN
cord-277909-rn1dow26	256	54	the	the	DT
cord-277909-rn1dow26	256	55	efficiency	efficiency	NN
cord-277909-rn1dow26	256	56	of	of	IN
cord-277909-rn1dow26	256	57	the	the	DT
cord-277909-rn1dow26	256	58	reaction	reaction	NN
cord-277909-rn1dow26	256	59	)	)	-RRB-
cord-277909-rn1dow26	256	60	,	,	,
cord-277909-rn1dow26	256	61	and	and	CC
cord-277909-rn1dow26	256	62	then	then	RB
cord-277909-rn1dow26	256	63	aliquot	aliquot	VB
cord-277909-rn1dow26	256	64	this	this	DT
cord-277909-rn1dow26	256	65	into	into	IN
cord-277909-rn1dow26	256	66	volumes	volume	NNS
cord-277909-rn1dow26	256	67	sufficient	sufficient	JJ
cord-277909-rn1dow26	256	68	to	to	TO
cord-277909-rn1dow26	256	69	last	last	VB
cord-277909-rn1dow26	256	70	1	1	CD
cord-277909-rn1dow26	256	71	week	week	NN
cord-277909-rn1dow26	256	72	at	at	IN
cord-277909-rn1dow26	256	73	4	4	CD
cord-277909-rn1dow26	256	74	•	•	NN
cord-277909-rn1dow26	257	1	C.	C.	NNP
cord-277909-rn1dow26	257	2	Aliquots	Aliquots	NNP
cord-277909-rn1dow26	257	3	are	be	VBP
cord-277909-rn1dow26	257	4	stored	store	VBN
cord-277909-rn1dow26	257	5	frozen	frozen	JJ
cord-277909-rn1dow26	257	6	at	at	IN
cord-277909-rn1dow26	257	7	−20	−20	NNP
cord-277909-rn1dow26	257	8	•	•	NNP
cord-277909-rn1dow26	257	9	C	C	NNP
cord-277909-rn1dow26	257	10	until	until	IN
cord-277909-rn1dow26	257	11	required	require	VBN
cord-277909-rn1dow26	257	12	.	.	.
cord-277909-rn1dow26	258	1	It	-PRON-	PRP
cord-277909-rn1dow26	258	2	is	be	VBZ
cord-277909-rn1dow26	258	3	essential	essential	JJ
cord-277909-rn1dow26	258	4	to	to	TO
cord-277909-rn1dow26	258	5	track	track	VB
cord-277909-rn1dow26	258	6	the	the	DT
cord-277909-rn1dow26	258	7	C	c	NN
cord-277909-rn1dow26	258	8	T	T	NNP
cord-277909-rn1dow26	258	9	values	value	NNS
cord-277909-rn1dow26	258	10	of	of	IN
cord-277909-rn1dow26	258	11	the	the	DT
cord-277909-rn1dow26	258	12	controls	control	NNS
cord-277909-rn1dow26	258	13	to	to	TO
cord-277909-rn1dow26	258	14	check	check	VB
cord-277909-rn1dow26	258	15	that	that	IN
cord-277909-rn1dow26	258	16	the	the	DT
cord-277909-rn1dow26	258	17	assay	assay	NN
cord-277909-rn1dow26	258	18	is	be	VBZ
cord-277909-rn1dow26	258	19	performing	perform	VBG
cord-277909-rn1dow26	258	20	satisfactorily	satisfactorily	RB
cord-277909-rn1dow26	258	21	,	,	,
cord-277909-rn1dow26	258	22	and	and	CC
cord-277909-rn1dow26	258	23	to	to	TO
cord-277909-rn1dow26	258	24	enable	enable	VB
cord-277909-rn1dow26	258	25	a	a	DT
cord-277909-rn1dow26	258	26	smooth	smooth	JJ
cord-277909-rn1dow26	258	27	transition	transition	NN
cord-277909-rn1dow26	258	28	to	to	IN
cord-277909-rn1dow26	258	29	a	a	DT
cord-277909-rn1dow26	258	30	new	new	JJ
cord-277909-rn1dow26	258	31	control	control	NN
cord-277909-rn1dow26	258	32	set	set	VBN
cord-277909-rn1dow26	258	33	when	when	WRB
cord-277909-rn1dow26	258	34	required	require	VBN
cord-277909-rn1dow26	258	35	(	(	-LRB-
cord-277909-rn1dow26	258	36	Fig	Fig	NNP
cord-277909-rn1dow26	258	37	.	.	NNP
cord-277909-rn1dow26	258	38	7	7	CD
cord-277909-rn1dow26	258	39	)	)	-RRB-
cord-277909-rn1dow26	258	40	.	.	.
cord-277909-rn1dow26	259	1	We	-PRON-	PRP
cord-277909-rn1dow26	259	2	record	record	VBP
cord-277909-rn1dow26	259	3	our	-PRON-	PRP$
cord-277909-rn1dow26	259	4	C	c	NN
cord-277909-rn1dow26	259	5	T	t	NN
cord-277909-rn1dow26	259	6	values	value	NNS
cord-277909-rn1dow26	259	7	in	in	IN
cord-277909-rn1dow26	259	8	the	the	DT
cord-277909-rn1dow26	259	9	form	form	NN
cord-277909-rn1dow26	259	10	of	of	IN
cord-277909-rn1dow26	259	11	a	a	DT
cord-277909-rn1dow26	259	12	Shewart	Shewart	NNP
cord-277909-rn1dow26	259	13	Control	Control	NNP
cord-277909-rn1dow26	259	14	Chart	Chart	NNP
cord-277909-rn1dow26	259	15	(	(	-LRB-
cord-277909-rn1dow26	259	16	Davies	Davies	NNP
cord-277909-rn1dow26	259	17	,	,	,
cord-277909-rn1dow26	259	18	2003	2003	CD
cord-277909-rn1dow26	259	19	)	)	-RRB-
cord-277909-rn1dow26	259	20	.	.	.
cord-277909-rn1dow26	260	1	Newly	newly	RB
cord-277909-rn1dow26	260	2	prepared	prepare	VBN
cord-277909-rn1dow26	260	3	standards	standard	NNS
cord-277909-rn1dow26	260	4	(	(	-LRB-
cord-277909-rn1dow26	260	5	produced	produce	VBN
cord-277909-rn1dow26	260	6	annually	annually	RB
cord-277909-rn1dow26	260	7	)	)	-RRB-
cord-277909-rn1dow26	260	8	must	must	MD
cord-277909-rn1dow26	260	9	have	have	VB
cord-277909-rn1dow26	260	10	C	C	NNP
cord-277909-rn1dow26	260	11	T	t	NN
cord-277909-rn1dow26	260	12	values	value	NNS
cord-277909-rn1dow26	260	13	falling	fall	VBG
cord-277909-rn1dow26	260	14	within	within	IN
cord-277909-rn1dow26	260	15	two	two	CD
cord-277909-rn1dow26	260	16	standard	standard	JJ
cord-277909-rn1dow26	260	17	deviation	deviation	NN
cord-277909-rn1dow26	260	18	of	of	IN
cord-277909-rn1dow26	260	19	the	the	DT
cord-277909-rn1dow26	260	20	mean	mean	JJ
cord-277909-rn1dow26	260	21	value	value	NN
cord-277909-rn1dow26	260	22	determined	determine	VBN
cord-277909-rn1dow26	260	23	for	for	IN
cord-277909-rn1dow26	260	24	the	the	DT
cord-277909-rn1dow26	260	25	standards	standard	NNS
cord-277909-rn1dow26	260	26	previously	previously	RB
cord-277909-rn1dow26	260	27	in	in	IN
cord-277909-rn1dow26	260	28	use	use	NN
cord-277909-rn1dow26	260	29	.	.	.
cord-277909-rn1dow26	261	1	A	a	DT
cord-277909-rn1dow26	261	2	second	second	JJ
cord-277909-rn1dow26	261	3	issue	issue	NN
cord-277909-rn1dow26	261	4	with	with	IN
cord-277909-rn1dow26	261	5	quantitative	quantitative	JJ
cord-277909-rn1dow26	261	6	assays	assay	NNS
cord-277909-rn1dow26	261	7	that	that	WDT
cord-277909-rn1dow26	261	8	do	do	VBP
cord-277909-rn1dow26	261	9	not	not	RB
cord-277909-rn1dow26	261	10	use	use	VB
cord-277909-rn1dow26	261	11	extracted	extract	VBN
cord-277909-rn1dow26	261	12	material	material	NN
cord-277909-rn1dow26	261	13	as	as	IN
cord-277909-rn1dow26	261	14	quantitative	quantitative	JJ
cord-277909-rn1dow26	261	15	controls	control	NNS
cord-277909-rn1dow26	261	16	is	be	VBZ
cord-277909-rn1dow26	261	17	that	that	IN
cord-277909-rn1dow26	261	18	these	these	DT
cord-277909-rn1dow26	261	19	assays	assay	NNS
cord-277909-rn1dow26	261	20	are	be	VBP
cord-277909-rn1dow26	261	21	sensitive	sensitive	JJ
cord-277909-rn1dow26	261	22	to	to	IN
cord-277909-rn1dow26	261	23	changes	change	NNS
cord-277909-rn1dow26	261	24	in	in	IN
cord-277909-rn1dow26	261	25	extraction	extraction	NN
cord-277909-rn1dow26	261	26	methodology	methodology	NN
cord-277909-rn1dow26	261	27	or	or	CC
cord-277909-rn1dow26	261	28	efficiency	efficiency	NN
cord-277909-rn1dow26	261	29	.	.	.
cord-277909-rn1dow26	262	1	We	-PRON-	PRP
cord-277909-rn1dow26	262	2	have	have	VBP
cord-277909-rn1dow26	262	3	recently	recently	RB
cord-277909-rn1dow26	262	4	moved	move	VBN
cord-277909-rn1dow26	262	5	to	to	IN
cord-277909-rn1dow26	262	6	a	a	DT
cord-277909-rn1dow26	262	7	more	more	RBR
cord-277909-rn1dow26	262	8	efficient	efficient	JJ
cord-277909-rn1dow26	262	9	extraction	extraction	NN
cord-277909-rn1dow26	262	10	kit	kit	NN
cord-277909-rn1dow26	262	11	(	(	-LRB-
cord-277909-rn1dow26	262	12	QIAamp	QIAamp	NNP
cord-277909-rn1dow26	262	13	Virus	Virus	NNP
cord-277909-rn1dow26	262	14	Robot	Robot	NNP
cord-277909-rn1dow26	262	15	kit	kit	NN
cord-277909-rn1dow26	262	16	)	)	-RRB-
cord-277909-rn1dow26	262	17	,	,	,
cord-277909-rn1dow26	262	18	but	but	CC
cord-277909-rn1dow26	262	19	as	as	IN
cord-277909-rn1dow26	262	20	our	-PRON-	PRP$
cord-277909-rn1dow26	262	21	standards	standard	NNS
cord-277909-rn1dow26	262	22	are	be	VBP
cord-277909-rn1dow26	262	23	plasmid	plasmid	JJ
cord-277909-rn1dow26	262	24	or	or	CC
cord-277909-rn1dow26	262	25	cellular	cellular	JJ
cord-277909-rn1dow26	262	26	DNA	dna	NN
cord-277909-rn1dow26	262	27	based	base	VBN
cord-277909-rn1dow26	262	28	and	and	CC
cord-277909-rn1dow26	262	29	are	be	VBP
cord-277909-rn1dow26	262	30	not	not	RB
cord-277909-rn1dow26	262	31	extracted	extract	VBN
cord-277909-rn1dow26	262	32	alongside	alongside	IN
cord-277909-rn1dow26	262	33	the	the	DT
cord-277909-rn1dow26	262	34	specimens	specimen	NNS
cord-277909-rn1dow26	262	35	,	,	,
cord-277909-rn1dow26	262	36	we	-PRON-	PRP
cord-277909-rn1dow26	262	37	are	be	VBP
cord-277909-rn1dow26	262	38	now	now	RB
cord-277909-rn1dow26	262	39	reporting	report	VBG
cord-277909-rn1dow26	262	40	higher	high	JJR
cord-277909-rn1dow26	262	41	viral	viral	JJ
cord-277909-rn1dow26	262	42	loads	load	NNS
cord-277909-rn1dow26	262	43	for	for	IN
cord-277909-rn1dow26	262	44	the	the	DT
cord-277909-rn1dow26	262	45	same	same	JJ
cord-277909-rn1dow26	262	46	sample	sample	NN
cord-277909-rn1dow26	262	47	than	than	IN
cord-277909-rn1dow26	262	48	with	with	IN
cord-277909-rn1dow26	262	49	previous	previous	JJ
cord-277909-rn1dow26	262	50	extraction	extraction	NN
cord-277909-rn1dow26	262	51	procedures	procedure	NNS
cord-277909-rn1dow26	262	52	.	.	.
cord-277909-rn1dow26	263	1	This	this	DT
cord-277909-rn1dow26	263	2	observed	observed	JJ
cord-277909-rn1dow26	263	3	change	change	NN
cord-277909-rn1dow26	263	4	in	in	IN
cord-277909-rn1dow26	263	5	viral	viral	JJ
cord-277909-rn1dow26	263	6	loads	load	NNS
cord-277909-rn1dow26	263	7	is	be	VBZ
cord-277909-rn1dow26	263	8	only	only	RB
cord-277909-rn1dow26	263	9	a	a	DT
cord-277909-rn1dow26	263	10	problem	problem	NN
cord-277909-rn1dow26	263	11	during	during	IN
cord-277909-rn1dow26	263	12	the	the	DT
cord-277909-rn1dow26	263	13	crossover	crossover	NN
cord-277909-rn1dow26	263	14	period	period	NN
cord-277909-rn1dow26	263	15	from	from	IN
cord-277909-rn1dow26	263	16	one	one	CD
cord-277909-rn1dow26	263	17	extraction	extraction	NN
cord-277909-rn1dow26	263	18	procedure	procedure	NN
cord-277909-rn1dow26	263	19	to	to	IN
cord-277909-rn1dow26	263	20	another	another	DT
cord-277909-rn1dow26	263	21	,	,	,
cord-277909-rn1dow26	263	22	as	as	IN
cord-277909-rn1dow26	263	23	subsequent	subsequent	JJ
cord-277909-rn1dow26	263	24	samples	sample	NNS
cord-277909-rn1dow26	263	25	will	will	MD
cord-277909-rn1dow26	263	26	be	be	VB
cord-277909-rn1dow26	263	27	analysed	analyse	VBN
cord-277909-rn1dow26	263	28	in	in	IN
cord-277909-rn1dow26	263	29	the	the	DT
cord-277909-rn1dow26	263	30	light	light	NN
cord-277909-rn1dow26	263	31	of	of	IN
cord-277909-rn1dow26	263	32	the	the	DT
cord-277909-rn1dow26	263	33	new	new	JJ
cord-277909-rn1dow26	263	34	baseline	baseline	JJ
cord-277909-rn1dow26	263	35	level	level	NN
cord-277909-rn1dow26	263	36	.	.	.
cord-277909-rn1dow26	264	1	To	to	TO
cord-277909-rn1dow26	264	2	ensure	ensure	VB
cord-277909-rn1dow26	264	3	intra	intra	JJ
cord-277909-rn1dow26	264	4	-	-	JJ
cord-277909-rn1dow26	264	5	run	run	JJ
cord-277909-rn1dow26	264	6	extraction	extraction	NN
cord-277909-rn1dow26	264	7	consistency	consistency	NN
cord-277909-rn1dow26	264	8	,	,	,
cord-277909-rn1dow26	264	9	a	a	DT
cord-277909-rn1dow26	264	10	positive	positive	JJ
cord-277909-rn1dow26	264	11	or	or	CC
cord-277909-rn1dow26	264	12	an	an	DT
cord-277909-rn1dow26	264	13	internal	internal	JJ
cord-277909-rn1dow26	264	14	control	control	NN
cord-277909-rn1dow26	264	15	(	(	-LRB-
cord-277909-rn1dow26	264	16	of	of	IN
cord-277909-rn1dow26	264	17	known	know	VBN
cord-277909-rn1dow26	264	18	quantity	quantity	NN
cord-277909-rn1dow26	264	19	)	)	-RRB-
cord-277909-rn1dow26	264	20	should	should	MD
cord-277909-rn1dow26	264	21	be	be	VB
cord-277909-rn1dow26	264	22	extracted	extract	VBN
cord-277909-rn1dow26	264	23	and	and	CC
cord-277909-rn1dow26	264	24	run	run	VBN
cord-277909-rn1dow26	264	25	at	at	IN
cord-277909-rn1dow26	264	26	the	the	DT
cord-277909-rn1dow26	264	27	same	same	JJ
cord-277909-rn1dow26	264	28	time	time	NN
cord-277909-rn1dow26	264	29	as	as	IN
cord-277909-rn1dow26	264	30	the	the	DT
cord-277909-rn1dow26	264	31	samples	sample	NNS
cord-277909-rn1dow26	264	32	to	to	TO
cord-277909-rn1dow26	264	33	be	be	VB
cord-277909-rn1dow26	264	34	tested	test	VBN
cord-277909-rn1dow26	264	35	.	.	.
cord-277909-rn1dow26	265	1	This	this	DT
cord-277909-rn1dow26	265	2	control	control	NN
cord-277909-rn1dow26	265	3	should	should	MD
cord-277909-rn1dow26	265	4	be	be	VB
cord-277909-rn1dow26	265	5	monitored	monitor	VBN
cord-277909-rn1dow26	265	6	in	in	IN
cord-277909-rn1dow26	265	7	the	the	DT
cord-277909-rn1dow26	265	8	same	same	JJ
cord-277909-rn1dow26	265	9	way	way	NN
cord-277909-rn1dow26	265	10	as	as	IN
cord-277909-rn1dow26	265	11	outlined	outline	VBN
cord-277909-rn1dow26	265	12	above	above	RB
cord-277909-rn1dow26	265	13	(	(	-LRB-
cord-277909-rn1dow26	265	14	see	see	VB
cord-277909-rn1dow26	265	15	routine	routine	JJ
cord-277909-rn1dow26	265	16	validation	validation	NN
cord-277909-rn1dow26	265	17	of	of	IN
cord-277909-rn1dow26	265	18	each	each	DT
cord-277909-rn1dow26	265	19	real	real	JJ
cord-277909-rn1dow26	265	20	time	time	NN
cord-277909-rn1dow26	265	21	PCR	PCR	NNP
cord-277909-rn1dow26	265	22	run	run	NN
cord-277909-rn1dow26	265	23	)	)	-RRB-
cord-277909-rn1dow26	265	24	.	.	.
cord-277909-rn1dow26	266	1	Once	once	RB
cord-277909-rn1dow26	266	2	an	an	DT
cord-277909-rn1dow26	266	3	assay	assay	NN
cord-277909-rn1dow26	266	4	(	(	-LRB-
cord-277909-rn1dow26	266	5	or	or	CC
cord-277909-rn1dow26	266	6	a	a	DT
cord-277909-rn1dow26	266	7	number	number	NN
cord-277909-rn1dow26	266	8	of	of	IN
cord-277909-rn1dow26	266	9	assays	assay	NNS
cord-277909-rn1dow26	266	10	)	)	-RRB-
cord-277909-rn1dow26	266	11	has	have	VBZ
cord-277909-rn1dow26	266	12	been	be	VBN
cord-277909-rn1dow26	266	13	introduced	introduce	VBN
cord-277909-rn1dow26	266	14	into	into	IN
cord-277909-rn1dow26	266	15	routine	routine	JJ
cord-277909-rn1dow26	266	16	service	service	NN
cord-277909-rn1dow26	266	17	it	-PRON-	PRP
cord-277909-rn1dow26	266	18	is	be	VBZ
cord-277909-rn1dow26	266	19	important	important	JJ
cord-277909-rn1dow26	267	1	to	to	TO
cord-277909-rn1dow26	267	2	re	re	VB
cord-277909-rn1dow26	267	3	-	-	VB
cord-277909-rn1dow26	267	4	assess	assess	VB
cord-277909-rn1dow26	267	5	the	the	DT
cord-277909-rn1dow26	267	6	sensitivity	sensitivity	NN
cord-277909-rn1dow26	267	7	of	of	IN
cord-277909-rn1dow26	267	8	the	the	DT
cord-277909-rn1dow26	267	9	assay	assay	NN
cord-277909-rn1dow26	267	10	in	in	IN
cord-277909-rn1dow26	267	11	relation	relation	NN
cord-277909-rn1dow26	267	12	to	to	IN
cord-277909-rn1dow26	267	13	current	current	JJ
cord-277909-rn1dow26	267	14	circulating	circulate	VBG
cord-277909-rn1dow26	267	15	viruses	virus	NNS
cord-277909-rn1dow26	267	16	.	.	.
cord-277909-rn1dow26	268	1	This	this	DT
cord-277909-rn1dow26	268	2	can	can	MD
cord-277909-rn1dow26	268	3	Fig	Fig	NNP
cord-277909-rn1dow26	268	4	.	.	NNP
cord-277909-rn1dow26	268	5	7	7	CD
cord-277909-rn1dow26	268	6	.	.	.
cord-277909-rn1dow26	269	1	Application	application	NN
cord-277909-rn1dow26	269	2	of	of	IN
cord-277909-rn1dow26	269	3	Shewart	Shewart	NNP
cord-277909-rn1dow26	269	4	Control	Control	NNP
cord-277909-rn1dow26	269	5	Chart	Chart	NNP
cord-277909-rn1dow26	269	6	to	to	TO
cord-277909-rn1dow26	269	7	track	track	VB
cord-277909-rn1dow26	269	8	potential	potential	JJ
cord-277909-rn1dow26	269	9	changes	change	NNS
cord-277909-rn1dow26	269	10	in	in	IN
cord-277909-rn1dow26	269	11	assay	assay	NN
cord-277909-rn1dow26	269	12	performance	performance	NN
cord-277909-rn1dow26	269	13	.	.	.
cord-277909-rn1dow26	270	1	The	the	DT
cord-277909-rn1dow26	270	2	mean	mean	JJ
cord-277909-rn1dow26	270	3	CT	CT	NNP
cord-277909-rn1dow26	270	4	values	value	NNS
cord-277909-rn1dow26	270	5	obtained	obtain	VBN
cord-277909-rn1dow26	270	6	for	for	IN
cord-277909-rn1dow26	270	7	the	the	DT
cord-277909-rn1dow26	270	8	1	1	CD
cord-277909-rn1dow26	270	9	×	×	NNP
cord-277909-rn1dow26	270	10	10	10	CD
cord-277909-rn1dow26	270	11	7	7	CD
cord-277909-rn1dow26	270	12	copies	copy	NNS
cord-277909-rn1dow26	270	13	per	per	IN
cord-277909-rn1dow26	270	14	ml	ml	NN
cord-277909-rn1dow26	270	15	standard	standard	NN
cord-277909-rn1dow26	270	16	were	be	VBD
cord-277909-rn1dow26	270	17	plotted	plot	VBN
cord-277909-rn1dow26	270	18	over	over	IN
cord-277909-rn1dow26	270	19	time	time	NN
cord-277909-rn1dow26	270	20	.	.	.
cord-277909-rn1dow26	271	1	The	the	DT
cord-277909-rn1dow26	271	2	average	average	JJ
cord-277909-rn1dow26	271	3	value	value	NN
cord-277909-rn1dow26	271	4	of	of	IN
cord-277909-rn1dow26	271	5	these	these	DT
cord-277909-rn1dow26	271	6	CT	CT	NNP
cord-277909-rn1dow26	271	7	values	value	NNS
cord-277909-rn1dow26	271	8	was	be	VBD
cord-277909-rn1dow26	271	9	calculated	calculate	VBN
cord-277909-rn1dow26	271	10	and	and	CC
cord-277909-rn1dow26	271	11	plotted	plot	VBN
cord-277909-rn1dow26	271	12	(	(	-LRB-
cord-277909-rn1dow26	271	13	red	red	JJ
cord-277909-rn1dow26	271	14	line	line	NN
cord-277909-rn1dow26	271	15	)	)	-RRB-
cord-277909-rn1dow26	271	16	for	for	IN
cord-277909-rn1dow26	271	17	each	each	DT
cord-277909-rn1dow26	271	18	data	data	NN
cord-277909-rn1dow26	271	19	set	set	VBN
cord-277909-rn1dow26	271	20	(	(	-LRB-
cord-277909-rn1dow26	271	21	along	along	IN
cord-277909-rn1dow26	271	22	with	with	IN
cord-277909-rn1dow26	271	23	two	two	CD
cord-277909-rn1dow26	271	24	standard	standard	JJ
cord-277909-rn1dow26	271	25	deviations	deviation	NNS
cord-277909-rn1dow26	271	26	above	above	IN
cord-277909-rn1dow26	271	27	(	(	-LRB-
cord-277909-rn1dow26	271	28	pink	pink	JJ
cord-277909-rn1dow26	271	29	line	line	NN
cord-277909-rn1dow26	271	30	)	)	-RRB-
cord-277909-rn1dow26	271	31	and	and	CC
cord-277909-rn1dow26	271	32	below	below	IN
cord-277909-rn1dow26	271	33	(	(	-LRB-
cord-277909-rn1dow26	271	34	blue	blue	JJ
cord-277909-rn1dow26	271	35	line	line	NN
cord-277909-rn1dow26	271	36	)	)	-RRB-
cord-277909-rn1dow26	271	37	the	the	DT
cord-277909-rn1dow26	271	38	average	average	JJ
cord-277909-rn1dow26	271	39	value	value	NN
cord-277909-rn1dow26	271	40	)	)	-RRB-
cord-277909-rn1dow26	271	41	.	.	.
cord-277909-rn1dow26	272	1	Two	two	CD
cord-277909-rn1dow26	272	2	standard	standard	JJ
cord-277909-rn1dow26	272	3	deviations	deviation	NNS
cord-277909-rn1dow26	272	4	are	be	VBP
cord-277909-rn1dow26	272	5	generally	generally	RB
cord-277909-rn1dow26	272	6	accepted	accept	VBN
cord-277909-rn1dow26	272	7	as	as	IN
cord-277909-rn1dow26	272	8	the	the	DT
cord-277909-rn1dow26	272	9	warning	warning	NN
cord-277909-rn1dow26	272	10	level	level	NN
cord-277909-rn1dow26	272	11	in	in	IN
cord-277909-rn1dow26	272	12	such	such	JJ
cord-277909-rn1dow26	272	13	analyses	analysis	NNS
cord-277909-rn1dow26	272	14	.	.	.
cord-277909-rn1dow26	273	1	The	the	DT
cord-277909-rn1dow26	273	2	first	first	JJ
cord-277909-rn1dow26	273	3	'	'	``
cord-277909-rn1dow26	273	4	jump	jump	NN
cord-277909-rn1dow26	273	5	'	'	''
cord-277909-rn1dow26	273	6	(	(	-LRB-
cord-277909-rn1dow26	273	7	A	a	NN
cord-277909-rn1dow26	273	8	)	)	-RRB-
cord-277909-rn1dow26	273	9	represents	represent	VBZ
cord-277909-rn1dow26	273	10	a	a	DT
cord-277909-rn1dow26	273	11	change	change	NN
cord-277909-rn1dow26	273	12	in	in	IN
cord-277909-rn1dow26	273	13	the	the	DT
cord-277909-rn1dow26	273	14	set	set	NN
cord-277909-rn1dow26	273	15	of	of	IN
cord-277909-rn1dow26	273	16	standards	standard	NNS
cord-277909-rn1dow26	273	17	used	use	VBN
cord-277909-rn1dow26	273	18	,	,	,
cord-277909-rn1dow26	273	19	and	and	CC
cord-277909-rn1dow26	273	20	while	while	IN
cord-277909-rn1dow26	273	21	this	this	DT
cord-277909-rn1dow26	273	22	is	be	VBZ
cord-277909-rn1dow26	273	23	not	not	RB
cord-277909-rn1dow26	273	24	ideal	ideal	JJ
cord-277909-rn1dow26	273	25	,	,	,
cord-277909-rn1dow26	273	26	results	result	VBZ
cord-277909-rn1dow26	273	27	in	in	IN
cord-277909-rn1dow26	273	28	a	a	DT
cord-277909-rn1dow26	273	29	much	much	RB
cord-277909-rn1dow26	273	30	more	more	RBR
cord-277909-rn1dow26	273	31	reproducible	reproducible	JJ
cord-277909-rn1dow26	273	32	assay	assay	NN
cord-277909-rn1dow26	273	33	.	.	.
cord-277909-rn1dow26	274	1	The	the	DT
cord-277909-rn1dow26	274	2	second	second	JJ
cord-277909-rn1dow26	274	3	jump	jump	NN
cord-277909-rn1dow26	274	4	(	(	-LRB-
cord-277909-rn1dow26	274	5	B	b	NN
cord-277909-rn1dow26	274	6	)	)	-RRB-
cord-277909-rn1dow26	274	7	is	be	VBZ
cord-277909-rn1dow26	274	8	caused	cause	VBN
cord-277909-rn1dow26	274	9	by	by	IN
cord-277909-rn1dow26	274	10	a	a	DT
cord-277909-rn1dow26	274	11	change	change	NN
cord-277909-rn1dow26	274	12	in	in	IN
cord-277909-rn1dow26	274	13	the	the	DT
cord-277909-rn1dow26	274	14	primer	primer	NN
cord-277909-rn1dow26	274	15	-	-	HYPH
cord-277909-rn1dow26	274	16	probe	probe	JJ
cord-277909-rn1dow26	274	17	pool	pool	NN
cord-277909-rn1dow26	274	18	in	in	IN
cord-277909-rn1dow26	274	19	use	use	NN
cord-277909-rn1dow26	274	20	,	,	,
cord-277909-rn1dow26	274	21	and	and	CC
cord-277909-rn1dow26	274	22	shows	show	VBZ
cord-277909-rn1dow26	274	23	a	a	DT
cord-277909-rn1dow26	274	24	significant	significant	JJ
cord-277909-rn1dow26	274	25	change	change	NN
cord-277909-rn1dow26	274	26	in	in	IN
cord-277909-rn1dow26	274	27	the	the	DT
cord-277909-rn1dow26	274	28	sensitivity	sensitivity	NN
cord-277909-rn1dow26	274	29	of	of	IN
cord-277909-rn1dow26	274	30	the	the	DT
cord-277909-rn1dow26	274	31	assay	assay	NN
cord-277909-rn1dow26	274	32	.	.	.
cord-277909-rn1dow26	275	1	As	as	IN
cord-277909-rn1dow26	275	2	a	a	DT
cord-277909-rn1dow26	275	3	result	result	NN
cord-277909-rn1dow26	275	4	of	of	IN
cord-277909-rn1dow26	275	5	this	this	DT
cord-277909-rn1dow26	275	6	analysis	analysis	NN
cord-277909-rn1dow26	275	7	another	another	DT
cord-277909-rn1dow26	275	8	batch	batch	NN
cord-277909-rn1dow26	275	9	of	of	IN
cord-277909-rn1dow26	275	10	primer	primer	NN
cord-277909-rn1dow26	275	11	-	-	HYPH
cord-277909-rn1dow26	275	12	probe	probe	NN
cord-277909-rn1dow26	275	13	pool	pool	NN
cord-277909-rn1dow26	275	14	was	be	VBD
cord-277909-rn1dow26	275	15	prepared	prepare	VBN
cord-277909-rn1dow26	275	16	and	and	CC
cord-277909-rn1dow26	275	17	the	the	DT
cord-277909-rn1dow26	275	18	results	result	NNS
cord-277909-rn1dow26	275	19	obtained	obtain	VBN
cord-277909-rn1dow26	275	20	returned	return	VBN
cord-277909-rn1dow26	275	21	to	to	IN
cord-277909-rn1dow26	275	22	the	the	DT
cord-277909-rn1dow26	275	23	acceptable	acceptable	JJ
cord-277909-rn1dow26	275	24	range	range	NN
cord-277909-rn1dow26	275	25	.	.	.
cord-277909-rn1dow26	276	1	For	for	IN
cord-277909-rn1dow26	276	2	interpretation	interpretation	NN
cord-277909-rn1dow26	276	3	of	of	IN
cord-277909-rn1dow26	276	4	the	the	DT
cord-277909-rn1dow26	276	5	references	reference	NNS
cord-277909-rn1dow26	276	6	to	to	TO
cord-277909-rn1dow26	276	7	colour	colour	VB
cord-277909-rn1dow26	276	8	in	in	IN
cord-277909-rn1dow26	276	9	this	this	DT
cord-277909-rn1dow26	276	10	figure	figure	NN
cord-277909-rn1dow26	276	11	legend	legend	NN
cord-277909-rn1dow26	276	12	,	,	,
cord-277909-rn1dow26	276	13	the	the	DT
cord-277909-rn1dow26	276	14	reader	reader	NN
cord-277909-rn1dow26	276	15	is	be	VBZ
cord-277909-rn1dow26	276	16	referred	refer	VBN
cord-277909-rn1dow26	276	17	to	to	IN
cord-277909-rn1dow26	276	18	the	the	DT
cord-277909-rn1dow26	276	19	web	web	NN
cord-277909-rn1dow26	276	20	version	version	NN
cord-277909-rn1dow26	276	21	of	of	IN
cord-277909-rn1dow26	276	22	the	the	DT
cord-277909-rn1dow26	276	23	article	article	NN
cord-277909-rn1dow26	276	24	.	.	.
cord-277909-rn1dow26	277	1	be	be	VB
cord-277909-rn1dow26	277	2	carried	carry	VBN
cord-277909-rn1dow26	277	3	out	out	RP
cord-277909-rn1dow26	277	4	using	use	VBG
cord-277909-rn1dow26	277	5	positive	positive	JJ
cord-277909-rn1dow26	277	6	samples	sample	NNS
cord-277909-rn1dow26	277	7	detected	detect	VBN
cord-277909-rn1dow26	277	8	by	by	IN
cord-277909-rn1dow26	277	9	an	an	DT
cord-277909-rn1dow26	277	10	alternative	alternative	JJ
cord-277909-rn1dow26	277	11	test	test	NN
cord-277909-rn1dow26	277	12	or	or	CC
cord-277909-rn1dow26	277	13	by	by	IN
cord-277909-rn1dow26	277	14	comparing	compare	VBG
cord-277909-rn1dow26	277	15	primers	primer	NNS
cord-277909-rn1dow26	277	16	and	and	CC
cord-277909-rn1dow26	277	17	probe	probe	VB
cord-277909-rn1dow26	277	18	to	to	IN
cord-277909-rn1dow26	277	19	new	new	JJ
cord-277909-rn1dow26	277	20	sequences	sequence	NNS
cord-277909-rn1dow26	277	21	stored	store	VBN
cord-277909-rn1dow26	277	22	in	in	IN
cord-277909-rn1dow26	277	23	surveillance	surveillance	NN
cord-277909-rn1dow26	277	24	databases	database	NNS
cord-277909-rn1dow26	277	25	.	.	.
cord-277909-rn1dow26	278	1	Although	although	IN
cord-277909-rn1dow26	278	2	most	most	JJS
cord-277909-rn1dow26	278	3	assays	assay	NNS
cord-277909-rn1dow26	278	4	target	target	VBP
cord-277909-rn1dow26	278	5	a	a	DT
cord-277909-rn1dow26	278	6	conserved	conserved	JJ
cord-277909-rn1dow26	278	7	region	region	NN
cord-277909-rn1dow26	278	8	of	of	IN
cord-277909-rn1dow26	278	9	the	the	DT
cord-277909-rn1dow26	278	10	viral	viral	JJ
cord-277909-rn1dow26	278	11	genome	genome	NN
cord-277909-rn1dow26	278	12	,	,	,
cord-277909-rn1dow26	278	13	small	small	JJ
cord-277909-rn1dow26	278	14	changes	change	NNS
cord-277909-rn1dow26	278	15	in	in	IN
cord-277909-rn1dow26	278	16	the	the	DT
cord-277909-rn1dow26	278	17	target	target	NN
cord-277909-rn1dow26	278	18	can	can	MD
cord-277909-rn1dow26	278	19	result	result	VB
cord-277909-rn1dow26	278	20	in	in	IN
cord-277909-rn1dow26	278	21	false	false	JJ
cord-277909-rn1dow26	278	22	negative	negative	JJ
cord-277909-rn1dow26	278	23	reactions	reaction	NNS
cord-277909-rn1dow26	278	24	due	due	JJ
cord-277909-rn1dow26	278	25	to	to	TO
cord-277909-rn1dow26	278	26	primer	primer	VB
cord-277909-rn1dow26	278	27	and/or	and/or	CC
cord-277909-rn1dow26	278	28	probe	probe	NN
cord-277909-rn1dow26	278	29	mismatches	mismatch	NNS
cord-277909-rn1dow26	278	30	.	.	.
cord-277909-rn1dow26	279	1	If	if	IN
cord-277909-rn1dow26	279	2	a	a	DT
cord-277909-rn1dow26	279	3	loss	loss	NN
cord-277909-rn1dow26	279	4	in	in	IN
cord-277909-rn1dow26	279	5	sensitivity	sensitivity	NN
cord-277909-rn1dow26	279	6	occurs	occur	VBZ
cord-277909-rn1dow26	279	7	primer	primer	NN
cord-277909-rn1dow26	279	8	or	or	CC
cord-277909-rn1dow26	279	9	probe	probe	NN
cord-277909-rn1dow26	279	10	sequences	sequence	NNS
cord-277909-rn1dow26	279	11	may	may	MD
cord-277909-rn1dow26	279	12	need	need	VB
cord-277909-rn1dow26	279	13	to	to	TO
cord-277909-rn1dow26	279	14	be	be	VB
cord-277909-rn1dow26	279	15	updated	update	VBN
cord-277909-rn1dow26	279	16	or	or	CC
cord-277909-rn1dow26	279	17	a	a	DT
cord-277909-rn1dow26	279	18	new	new	JJ
cord-277909-rn1dow26	279	19	assay	assay	NN
cord-277909-rn1dow26	279	20	may	may	MD
cord-277909-rn1dow26	279	21	have	have	VB
cord-277909-rn1dow26	279	22	to	to	TO
cord-277909-rn1dow26	279	23	be	be	VB
cord-277909-rn1dow26	279	24	developed	develop	VBN
cord-277909-rn1dow26	279	25	.	.	.
cord-277909-rn1dow26	280	1	Interpreting	interpret	VBG
cord-277909-rn1dow26	280	2	real	real	JJ
cord-277909-rn1dow26	280	3	time	time	NN
cord-277909-rn1dow26	280	4	PCR	PCR	NNP
cord-277909-rn1dow26	280	5	results	result	NNS
cord-277909-rn1dow26	280	6	is	be	VBZ
cord-277909-rn1dow26	280	7	a	a	DT
cord-277909-rn1dow26	280	8	relatively	relatively	RB
cord-277909-rn1dow26	280	9	straightforward	straightforward	JJ
cord-277909-rn1dow26	280	10	process	process	NN
cord-277909-rn1dow26	280	11	.	.	.
cord-277909-rn1dow26	281	1	In	in	IN
cord-277909-rn1dow26	281	2	a	a	DT
cord-277909-rn1dow26	281	3	fully	fully	RB
cord-277909-rn1dow26	281	4	optimised	optimise	VBN
cord-277909-rn1dow26	281	5	assay	assay	NN
cord-277909-rn1dow26	281	6	all	all	DT
cord-277909-rn1dow26	281	7	positive	positive	JJ
cord-277909-rn1dow26	281	8	results	result	NNS
cord-277909-rn1dow26	281	9	should	should	MD
cord-277909-rn1dow26	281	10	show	show	VB
cord-277909-rn1dow26	281	11	increases	increase	NNS
cord-277909-rn1dow26	281	12	in	in	IN
cord-277909-rn1dow26	281	13	fluorescence	fluorescence	NN
cord-277909-rn1dow26	281	14	in	in	IN
cord-277909-rn1dow26	281	15	a	a	DT
cord-277909-rn1dow26	281	16	characteristic	characteristic	JJ
cord-277909-rn1dow26	281	17	exponential	exponential	JJ
cord-277909-rn1dow26	281	18	curve	curve	NN
cord-277909-rn1dow26	281	19	.	.	.
cord-277909-rn1dow26	282	1	However	however	RB
cord-277909-rn1dow26	282	2	there	there	EX
cord-277909-rn1dow26	282	3	are	be	VBP
cord-277909-rn1dow26	282	4	still	still	RB
cord-277909-rn1dow26	282	5	pitfalls	pitfall	NNS
cord-277909-rn1dow26	282	6	that	that	WDT
cord-277909-rn1dow26	282	7	we	-PRON-	PRP
cord-277909-rn1dow26	282	8	feel	feel	VBP
cord-277909-rn1dow26	282	9	users	user	NNS
cord-277909-rn1dow26	282	10	should	should	MD
cord-277909-rn1dow26	282	11	be	be	VB
cord-277909-rn1dow26	282	12	made	make	VBN
cord-277909-rn1dow26	282	13	aware	aware	JJ
cord-277909-rn1dow26	282	14	of	of	IN
cord-277909-rn1dow26	282	15	when	when	WRB
cord-277909-rn1dow26	282	16	interpreting	interpret	VBG
cord-277909-rn1dow26	282	17	data	datum	NNS
cord-277909-rn1dow26	282	18	.	.	.
cord-277909-rn1dow26	283	1	Occasionally	occasionally	RB
cord-277909-rn1dow26	283	2	samples	sample	NNS
cord-277909-rn1dow26	283	3	may	may	MD
cord-277909-rn1dow26	283	4	show	show	VB
cord-277909-rn1dow26	283	5	"	"	``
cord-277909-rn1dow26	283	6	signal	signal	NN
cord-277909-rn1dow26	283	7	drift	drift	NN
cord-277909-rn1dow26	283	8	"	"	''
cord-277909-rn1dow26	283	9	(	(	-LRB-
cord-277909-rn1dow26	283	10	traces	trace	NNS
cord-277909-rn1dow26	283	11	that	that	WDT
cord-277909-rn1dow26	283	12	increase	increase	VBP
cord-277909-rn1dow26	283	13	in	in	IN
cord-277909-rn1dow26	283	14	fluorescence	fluorescence	NN
cord-277909-rn1dow26	283	15	as	as	IN
cord-277909-rn1dow26	283	16	the	the	DT
cord-277909-rn1dow26	283	17	PCR	PCR	NNP
cord-277909-rn1dow26	283	18	progresses	progress	VBZ
cord-277909-rn1dow26	283	19	but	but	CC
cord-277909-rn1dow26	283	20	are	be	VBP
cord-277909-rn1dow26	283	21	not	not	RB
cord-277909-rn1dow26	283	22	exponential	exponential	JJ
cord-277909-rn1dow26	283	23	)	)	-RRB-
cord-277909-rn1dow26	283	24	(	(	-LRB-
cord-277909-rn1dow26	283	25	Fig	Fig	NNP
cord-277909-rn1dow26	283	26	.	.	.
cord-277909-rn1dow26	283	27	8)	8)	NNP
cord-277909-rn1dow26	283	28	.	.	.
cord-277909-rn1dow26	284	1	Signal	signal	NN
cord-277909-rn1dow26	284	2	drift	drift	NN
cord-277909-rn1dow26	284	3	can	can	MD
cord-277909-rn1dow26	284	4	be	be	VB
cord-277909-rn1dow26	284	5	produced	produce	VBN
cord-277909-rn1dow26	284	6	for	for	IN
cord-277909-rn1dow26	284	7	a	a	DT
cord-277909-rn1dow26	284	8	number	number	NN
cord-277909-rn1dow26	284	9	of	of	IN
cord-277909-rn1dow26	284	10	reasons	reason	NNS
cord-277909-rn1dow26	284	11	.	.	.
cord-277909-rn1dow26	285	1	True	true	JJ
cord-277909-rn1dow26	285	2	positive	positive	JJ
cord-277909-rn1dow26	285	3	samples	sample	NNS
cord-277909-rn1dow26	285	4	may	may	MD
cord-277909-rn1dow26	285	5	show	show	VB
cord-277909-rn1dow26	285	6	signal	signal	NN
cord-277909-rn1dow26	285	7	drift	drift	NN
cord-277909-rn1dow26	285	8	because	because	IN
cord-277909-rn1dow26	285	9	of	of	IN
cord-277909-rn1dow26	285	10	sub	sub	NN
cord-277909-rn1dow26	285	11	optimal	optimal	JJ
cord-277909-rn1dow26	285	12	PCR	PCR	NNP
cord-277909-rn1dow26	285	13	conditions	condition	NNS
cord-277909-rn1dow26	285	14	,	,	,
cord-277909-rn1dow26	285	15	inhibition	inhibition	NN
cord-277909-rn1dow26	285	16	and	and	CC
cord-277909-rn1dow26	285	17	primer	primer	NN
cord-277909-rn1dow26	285	18	mismatches	mismatch	NNS
cord-277909-rn1dow26	285	19	.	.	.
cord-277909-rn1dow26	286	1	Occasionally	occasionally	RB
cord-277909-rn1dow26	286	2	negative	negative	JJ
cord-277909-rn1dow26	286	3	samples	sample	NNS
cord-277909-rn1dow26	286	4	may	may	MD
cord-277909-rn1dow26	286	5	also	also	RB
cord-277909-rn1dow26	286	6	show	show	VB
cord-277909-rn1dow26	286	7	signal	signal	NN
cord-277909-rn1dow26	286	8	drift	drift	NN
cord-277909-rn1dow26	286	9	.	.	.
cord-277909-rn1dow26	287	1	This	this	DT
cord-277909-rn1dow26	287	2	may	may	MD
cord-277909-rn1dow26	287	3	be	be	VB
cord-277909-rn1dow26	287	4	due	due	JJ
cord-277909-rn1dow26	287	5	to	to	TO
cord-277909-rn1dow26	287	6	probe	probe	VB
cord-277909-rn1dow26	287	7	breakdown	breakdown	NN
cord-277909-rn1dow26	287	8	resulting	result	VBG
cord-277909-rn1dow26	287	9	in	in	IN
cord-277909-rn1dow26	287	10	a	a	DT
cord-277909-rn1dow26	287	11	fluorescence	fluorescence	NN
cord-277909-rn1dow26	287	12	increase	increase	NN
cord-277909-rn1dow26	287	13	.	.	.
cord-277909-rn1dow26	288	1	Signal	signal	NN
cord-277909-rn1dow26	288	2	drift	drift	NN
cord-277909-rn1dow26	288	3	often	often	RB
cord-277909-rn1dow26	288	4	occurs	occur	VBZ
cord-277909-rn1dow26	288	5	towards	towards	IN
cord-277909-rn1dow26	288	6	the	the	DT
cord-277909-rn1dow26	288	7	end	end	NN
cord-277909-rn1dow26	288	8	of	of	IN
cord-277909-rn1dow26	288	9	the	the	DT
cord-277909-rn1dow26	288	10	PCR	PCR	NNP
cord-277909-rn1dow26	288	11	reaction	reaction	NN
cord-277909-rn1dow26	288	12	.	.	.
cord-277909-rn1dow26	289	1	Some	some	DT
cord-277909-rn1dow26	289	2	platforms	platform	NNS
cord-277909-rn1dow26	289	3	allow	allow	VBP
cord-277909-rn1dow26	289	4	multicomponent	multicomponent	JJ
cord-277909-rn1dow26	289	5	analysis	analysis	NN
cord-277909-rn1dow26	289	6	of	of	IN
cord-277909-rn1dow26	289	7	weak	weak	JJ
cord-277909-rn1dow26	289	8	positive	positive	JJ
cord-277909-rn1dow26	289	9	traces	trace	NNS
cord-277909-rn1dow26	289	10	.	.	.
cord-277909-rn1dow26	290	1	This	this	DT
cord-277909-rn1dow26	290	2	allows	allow	VBZ
cord-277909-rn1dow26	290	3	users	user	NNS
cord-277909-rn1dow26	290	4	to	to	TO
cord-277909-rn1dow26	290	5	assess	assess	VB
cord-277909-rn1dow26	290	6	the	the	DT
cord-277909-rn1dow26	290	7	changes	change	NNS
cord-277909-rn1dow26	290	8	of	of	IN
cord-277909-rn1dow26	290	9	each	each	DT
cord-277909-rn1dow26	290	10	fluorescent	fluorescent	JJ
cord-277909-rn1dow26	290	11	label	label	NN
cord-277909-rn1dow26	290	12	in	in	IN
cord-277909-rn1dow26	290	13	the	the	DT
cord-277909-rn1dow26	290	14	reaction	reaction	NN
cord-277909-rn1dow26	290	15	.	.	.
cord-277909-rn1dow26	291	1	Genuine	genuine	JJ
cord-277909-rn1dow26	291	2	positive	positive	JJ
cord-277909-rn1dow26	291	3	traces	trace	NNS
cord-277909-rn1dow26	291	4	will	will	MD
cord-277909-rn1dow26	291	5	show	show	VB
cord-277909-rn1dow26	291	6	an	an	DT
cord-277909-rn1dow26	291	7	exponential	exponential	JJ
cord-277909-rn1dow26	291	8	increase	increase	NN
cord-277909-rn1dow26	291	9	in	in	IN
cord-277909-rn1dow26	291	10	the	the	DT
cord-277909-rn1dow26	291	11	fluorescent	fluorescent	JJ
cord-277909-rn1dow26	291	12	signal	signal	NN
cord-277909-rn1dow26	291	13	whereas	whereas	IN
cord-277909-rn1dow26	291	14	signal	signal	NN
cord-277909-rn1dow26	291	15	drift	drift	NN
cord-277909-rn1dow26	291	16	is	be	VBZ
cord-277909-rn1dow26	291	17	often	often	RB
cord-277909-rn1dow26	291	18	due	due	JJ
cord-277909-rn1dow26	291	19	to	to	IN
cord-277909-rn1dow26	291	20	a	a	DT
cord-277909-rn1dow26	291	21	change	change	NN
cord-277909-rn1dow26	291	22	in	in	IN
cord-277909-rn1dow26	291	23	the	the	DT
cord-277909-rn1dow26	291	24	normalisation	normalisation	NN
cord-277909-rn1dow26	291	25	dye	dye	NN
cord-277909-rn1dow26	291	26	(	(	-LRB-
cord-277909-rn1dow26	291	27	e.g.	e.g.	RB
cord-277909-rn1dow26	291	28	,	,	,
cord-277909-rn1dow26	291	29	ROX	ROX	NNP
cord-277909-rn1dow26	291	30	)	)	-RRB-
cord-277909-rn1dow26	291	31	.	.	.
cord-277909-rn1dow26	292	1	We	-PRON-	PRP
cord-277909-rn1dow26	292	2	currently	currently	RB
cord-277909-rn1dow26	292	3	repeat	repeat	VBP
cord-277909-rn1dow26	292	4	all	all	DT
cord-277909-rn1dow26	292	5	positive	positive	JJ
cord-277909-rn1dow26	292	6	samples	sample	NNS
cord-277909-rn1dow26	292	7	with	with	IN
cord-277909-rn1dow26	292	8	Ct	Ct	NNP
cord-277909-rn1dow26	292	9	's	's	POS
cord-277909-rn1dow26	292	10	greater	great	JJR
cord-277909-rn1dow26	292	11	than	than	IN
cord-277909-rn1dow26	292	12	35	35	CD
cord-277909-rn1dow26	292	13	cycles	cycle	NNS
cord-277909-rn1dow26	292	14	as	as	IN
cord-277909-rn1dow26	292	15	we	-PRON-	PRP
cord-277909-rn1dow26	292	16	feel	feel	VBP
cord-277909-rn1dow26	292	17	these	these	DT
cord-277909-rn1dow26	292	18	may	may	MD
cord-277909-rn1dow26	292	19	be	be	VB
cord-277909-rn1dow26	292	20	either	either	CC
cord-277909-rn1dow26	292	21	low	low	JJ
cord-277909-rn1dow26	292	22	copy	copy	NN
cord-277909-rn1dow26	292	23	number	number	NN
cord-277909-rn1dow26	292	24	positive	positive	JJ
cord-277909-rn1dow26	292	25	samples	sample	NNS
cord-277909-rn1dow26	292	26	or	or	CC
cord-277909-rn1dow26	292	27	non	non	JJ
cord-277909-rn1dow26	292	28	-	-	JJ
cord-277909-rn1dow26	292	29	specific	specific	JJ
cord-277909-rn1dow26	292	30	reactions	reaction	NNS
cord-277909-rn1dow26	292	31	.	.	.
cord-277909-rn1dow26	293	1	Some	some	DT
cord-277909-rn1dow26	293	2	96	96	CD
cord-277909-rn1dow26	293	3	well	well	UH
cord-277909-rn1dow26	293	4	real	real	JJ
cord-277909-rn1dow26	293	5	time	time	NN
cord-277909-rn1dow26	293	6	plates	plate	NNS
cord-277909-rn1dow26	293	7	require	require	VBP
cord-277909-rn1dow26	293	8	sealing	seal	VBG
cord-277909-rn1dow26	293	9	with	with	IN
cord-277909-rn1dow26	293	10	optically	optically	RB
cord-277909-rn1dow26	293	11	clear	clear	JJ
cord-277909-rn1dow26	293	12	plate	plate	NN
cord-277909-rn1dow26	293	13	seals	seal	NNS
cord-277909-rn1dow26	293	14	before	before	IN
cord-277909-rn1dow26	293	15	PCR	PCR	NNP
cord-277909-rn1dow26	293	16	can	can	MD
cord-277909-rn1dow26	293	17	take	take	VB
cord-277909-rn1dow26	293	18	place	place	NN
cord-277909-rn1dow26	293	19	.	.	.
cord-277909-rn1dow26	294	1	On	on	IN
cord-277909-rn1dow26	294	2	occasion	occasion	NN
cord-277909-rn1dow26	294	3	these	these	DT
cord-277909-rn1dow26	294	4	may	may	MD
cord-277909-rn1dow26	294	5	not	not	RB
cord-277909-rn1dow26	294	6	seal	seal	VB
cord-277909-rn1dow26	294	7	properly	properly	RB
cord-277909-rn1dow26	294	8	and	and	CC
cord-277909-rn1dow26	294	9	PCR	PCR	NNP
cord-277909-rn1dow26	294	10	reagents	reagent	NNS
cord-277909-rn1dow26	294	11	evaporate	evaporate	VBP
cord-277909-rn1dow26	294	12	during	during	IN
cord-277909-rn1dow26	294	13	cycling	cycling	NN
cord-277909-rn1dow26	294	14	.	.	.
cord-277909-rn1dow26	295	1	As	as	IN
cord-277909-rn1dow26	295	2	a	a	DT
cord-277909-rn1dow26	295	3	result	result	NN
cord-277909-rn1dow26	295	4	of	of	IN
cord-277909-rn1dow26	295	5	this	this	DT
cord-277909-rn1dow26	295	6	a	a	DT
cord-277909-rn1dow26	295	7	curve	curve	NN
cord-277909-rn1dow26	295	8	may	may	MD
cord-277909-rn1dow26	295	9	be	be	VB
cord-277909-rn1dow26	295	10	produced	produce	VBN
cord-277909-rn1dow26	295	11	mimicking	mimic	VBG
cord-277909-rn1dow26	295	12	a	a	DT
cord-277909-rn1dow26	295	13	positive	positive	JJ
cord-277909-rn1dow26	295	14	PCR	PCR	NNP
cord-277909-rn1dow26	295	15	reaction	reaction	NN
cord-277909-rn1dow26	295	16	.	.	.
cord-277909-rn1dow26	296	1	The	the	DT
cord-277909-rn1dow26	296	2	correct	correct	JJ
cord-277909-rn1dow26	296	3	placing	placing	NN
cord-277909-rn1dow26	296	4	of	of	IN
cord-277909-rn1dow26	296	5	the	the	DT
cord-277909-rn1dow26	296	6	threshold	threshold	NN
cord-277909-rn1dow26	296	7	line	line	NN
cord-277909-rn1dow26	296	8	is	be	VBZ
cord-277909-rn1dow26	296	9	essential	essential	JJ
cord-277909-rn1dow26	296	10	to	to	TO
cord-277909-rn1dow26	296	11	allow	allow	VB
cord-277909-rn1dow26	296	12	accurate	accurate	JJ
cord-277909-rn1dow26	296	13	C	c	NN
cord-277909-rn1dow26	296	14	T	t	NN
cord-277909-rn1dow26	296	15	measurement	measurement	NN
cord-277909-rn1dow26	296	16	.	.	.
cord-277909-rn1dow26	297	1	Some	some	DT
cord-277909-rn1dow26	297	2	of	of	IN
cord-277909-rn1dow26	297	3	the	the	DT
cord-277909-rn1dow26	297	4	computer	computer	NN
cord-277909-rn1dow26	297	5	software	software	NN
cord-277909-rn1dow26	297	6	available	available	JJ
cord-277909-rn1dow26	297	7	with	with	IN
cord-277909-rn1dow26	297	8	current	current	JJ
cord-277909-rn1dow26	297	9	real	real	JJ
cord-277909-rn1dow26	297	10	time	time	NN
cord-277909-rn1dow26	297	11	PCR	pcr	NN
cord-277909-rn1dow26	297	12	formats	format	NNS
cord-277909-rn1dow26	297	13	can	can	MD
cord-277909-rn1dow26	297	14	automatically	automatically	RB
cord-277909-rn1dow26	297	15	place	place	VB
cord-277909-rn1dow26	297	16	the	the	DT
cord-277909-rn1dow26	297	17	threshold	threshold	NN
cord-277909-rn1dow26	297	18	line	line	NN
cord-277909-rn1dow26	297	19	during	during	IN
cord-277909-rn1dow26	297	20	result	result	NN
cord-277909-rn1dow26	297	21	analysis	analysis	NN
cord-277909-rn1dow26	297	22	.	.	.
cord-277909-rn1dow26	298	1	Any	any	DT
cord-277909-rn1dow26	298	2	sample	sample	NN
cord-277909-rn1dow26	298	3	with	with	IN
cord-277909-rn1dow26	298	4	fluorescence	fluorescence	NN
cord-277909-rn1dow26	298	5	above	above	IN
cord-277909-rn1dow26	298	6	this	this	DT
cord-277909-rn1dow26	298	7	line	line	NN
cord-277909-rn1dow26	298	8	will	will	MD
cord-277909-rn1dow26	298	9	be	be	VB
cord-277909-rn1dow26	298	10	regarded	regard	VBN
cord-277909-rn1dow26	298	11	as	as	IN
cord-277909-rn1dow26	298	12	positive	positive	JJ
cord-277909-rn1dow26	298	13	by	by	IN
cord-277909-rn1dow26	298	14	the	the	DT
cord-277909-rn1dow26	298	15	computer	computer	NN
cord-277909-rn1dow26	298	16	.	.	.
cord-277909-rn1dow26	299	1	Always	always	RB
cord-277909-rn1dow26	299	2	check	check	VB
cord-277909-rn1dow26	299	3	the	the	DT
cord-277909-rn1dow26	299	4	automatic	automatic	JJ
cord-277909-rn1dow26	299	5	placement	placement	NN
cord-277909-rn1dow26	299	6	of	of	IN
cord-277909-rn1dow26	299	7	the	the	DT
cord-277909-rn1dow26	299	8	threshold	threshold	NN
cord-277909-rn1dow26	299	9	line	line	NN
cord-277909-rn1dow26	299	10	as	as	IN
cord-277909-rn1dow26	299	11	we	-PRON-	PRP
cord-277909-rn1dow26	299	12	have	have	VBP
cord-277909-rn1dow26	299	13	found	find	VBN
cord-277909-rn1dow26	299	14	that	that	IN
cord-277909-rn1dow26	299	15	sometimes	sometimes	RB
cord-277909-rn1dow26	299	16	the	the	DT
cord-277909-rn1dow26	299	17	computer	computer	NN
cord-277909-rn1dow26	299	18	will	will	MD
cord-277909-rn1dow26	299	19	place	place	VB
cord-277909-rn1dow26	299	20	it	-PRON-	PRP
cord-277909-rn1dow26	299	21	wrongly	wrongly	RB
cord-277909-rn1dow26	299	22	resulting	result	VBG
cord-277909-rn1dow26	299	23	in	in	IN
cord-277909-rn1dow26	299	24	both	both	DT
cord-277909-rn1dow26	299	25	false	false	JJ
cord-277909-rn1dow26	299	26	positive	positive	JJ
cord-277909-rn1dow26	299	27	and	and	CC
cord-277909-rn1dow26	299	28	negative	negative	JJ
cord-277909-rn1dow26	299	29	results	result	NNS
cord-277909-rn1dow26	299	30	(	(	-LRB-
cord-277909-rn1dow26	299	31	Fig	fig	NN
cord-277909-rn1dow26	299	32	.	.	.
cord-277909-rn1dow26	299	33	9	9	CD
cord-277909-rn1dow26	299	34	)	)	-RRB-
cord-277909-rn1dow26	299	35	.	.	.
cord-277909-rn1dow26	300	1	An	an	DT
cord-277909-rn1dow26	300	2	alternative	alternative	NN
cord-277909-rn1dow26	300	3	is	be	VBZ
cord-277909-rn1dow26	300	4	to	to	TO
cord-277909-rn1dow26	300	5	use	use	VB
cord-277909-rn1dow26	300	6	a	a	DT
cord-277909-rn1dow26	300	7	fixed	fix	VBN
cord-277909-rn1dow26	300	8	threshold	threshold	NN
cord-277909-rn1dow26	300	9	line	line	NN
cord-277909-rn1dow26	300	10	.	.	.
cord-277909-rn1dow26	301	1	The	the	DT
cord-277909-rn1dow26	301	2	use	use	NN
cord-277909-rn1dow26	301	3	of	of	IN
cord-277909-rn1dow26	301	4	such	such	PDT
cord-277909-rn1dow26	301	5	a	a	DT
cord-277909-rn1dow26	301	6	system	system	NN
cord-277909-rn1dow26	301	7	will	will	MD
cord-277909-rn1dow26	301	8	ensure	ensure	VB
cord-277909-rn1dow26	301	9	the	the	DT
cord-277909-rn1dow26	301	10	real	real	JJ
cord-277909-rn1dow26	301	11	time	time	NN
cord-277909-rn1dow26	301	12	assay	assay	NN
cord-277909-rn1dow26	301	13	is	be	VBZ
cord-277909-rn1dow26	301	14	directly	directly	RB
cord-277909-rn1dow26	301	15	comparable	comparable	JJ
cord-277909-rn1dow26	301	16	to	to	IN
cord-277909-rn1dow26	301	17	previous	previous	JJ
cord-277909-rn1dow26	301	18	runs	run	NNS
cord-277909-rn1dow26	302	1	This	this	DT
cord-277909-rn1dow26	302	2	should	should	MD
cord-277909-rn1dow26	302	3	not	not	RB
cord-277909-rn1dow26	302	4	preclude	preclude	VB
cord-277909-rn1dow26	302	5	careful	careful	JJ
cord-277909-rn1dow26	302	6	analysis	analysis	NN
cord-277909-rn1dow26	302	7	of	of	IN
cord-277909-rn1dow26	302	8	the	the	DT
cord-277909-rn1dow26	302	9	data	datum	NNS
cord-277909-rn1dow26	302	10	.	.	.
cord-277909-rn1dow26	303	1	The	the	DT
cord-277909-rn1dow26	303	2	increased	increase	VBN
cord-277909-rn1dow26	303	3	sensitivity	sensitivity	NN
cord-277909-rn1dow26	303	4	of	of	IN
cord-277909-rn1dow26	303	5	real	real	JJ
cord-277909-rn1dow26	303	6	time	time	NN
cord-277909-rn1dow26	303	7	PCR	PCR	NNP
cord-277909-rn1dow26	303	8	means	mean	VBZ
cord-277909-rn1dow26	303	9	that	that	IN
cord-277909-rn1dow26	303	10	,	,	,
cord-277909-rn1dow26	303	11	like	like	IN
cord-277909-rn1dow26	303	12	nested	nest	VBN
cord-277909-rn1dow26	303	13	PCR	PCR	NNP
cord-277909-rn1dow26	303	14	,	,	,
cord-277909-rn1dow26	303	15	occasionally	occasionally	RB
cord-277909-rn1dow26	303	16	positive	positive	JJ
cord-277909-rn1dow26	303	17	results	result	NNS
cord-277909-rn1dow26	303	18	will	will	MD
cord-277909-rn1dow26	303	19	be	be	VB
cord-277909-rn1dow26	303	20	obtained	obtain	VBN
cord-277909-rn1dow26	303	21	that	that	IN
cord-277909-rn1dow26	303	22	Fig	Fig	NNP
cord-277909-rn1dow26	303	23	.	.	.
cord-277909-rn1dow26	304	1	8	8	CD
cord-277909-rn1dow26	304	2	.	.	.
cord-277909-rn1dow26	305	1	Example	example	NN
cord-277909-rn1dow26	305	2	of	of	IN
cord-277909-rn1dow26	305	3	negative	negative	JJ
cord-277909-rn1dow26	305	4	samples	sample	NNS
cord-277909-rn1dow26	305	5	showing	show	VBG
cord-277909-rn1dow26	305	6	signal	signal	NN
cord-277909-rn1dow26	305	7	drift	drift	NN
cord-277909-rn1dow26	305	8	.	.	.
cord-277909-rn1dow26	306	1	The	the	DT
cord-277909-rn1dow26	306	2	two	two	CD
cord-277909-rn1dow26	306	3	sample	sample	NN
cord-277909-rn1dow26	306	4	shown	show	VBN
cord-277909-rn1dow26	306	5	in	in	IN
cord-277909-rn1dow26	306	6	blue	blue	JJ
cord-277909-rn1dow26	306	7	are	be	VBP
cord-277909-rn1dow26	306	8	showing	show	VBG
cord-277909-rn1dow26	306	9	an	an	DT
cord-277909-rn1dow26	306	10	increases	increase	NNS
cord-277909-rn1dow26	306	11	in	in	IN
cord-277909-rn1dow26	306	12	flourescence	flourescence	NN
cord-277909-rn1dow26	306	13	when	when	WRB
cord-277909-rn1dow26	306	14	examined	examine	VBN
cord-277909-rn1dow26	306	15	using	use	VBG
cord-277909-rn1dow26	306	16	the	the	DT
cord-277909-rn1dow26	306	17	quantification	quantification	NN
cord-277909-rn1dow26	306	18	option	option	NN
cord-277909-rn1dow26	306	19	(	(	-LRB-
cord-277909-rn1dow26	306	20	shown	show	VBN
cord-277909-rn1dow26	306	21	above	above	IN
cord-277909-rn1dow26	306	22	left	left	RB
cord-277909-rn1dow26	306	23	)	)	-RRB-
cord-277909-rn1dow26	306	24	.	.	.
cord-277909-rn1dow26	307	1	Analysis	analysis	NN
cord-277909-rn1dow26	307	2	of	of	IN
cord-277909-rn1dow26	307	3	the	the	DT
cord-277909-rn1dow26	307	4	raw	raw	JJ
cord-277909-rn1dow26	307	5	cycling	cycling	NN
cord-277909-rn1dow26	307	6	data	datum	NNS
cord-277909-rn1dow26	307	7	(	(	-LRB-
cord-277909-rn1dow26	307	8	shown	show	VBN
cord-277909-rn1dow26	307	9	above	above	IN
cord-277909-rn1dow26	307	10	right	right	NN
cord-277909-rn1dow26	307	11	)	)	-RRB-
cord-277909-rn1dow26	307	12	shows	show	VBZ
cord-277909-rn1dow26	307	13	that	that	IN
cord-277909-rn1dow26	307	14	there	there	EX
cord-277909-rn1dow26	307	15	is	be	VBZ
cord-277909-rn1dow26	307	16	no	no	DT
cord-277909-rn1dow26	307	17	increase	increase	NN
cord-277909-rn1dow26	307	18	in	in	IN
cord-277909-rn1dow26	307	19	flourescence	flourescence	NN
cord-277909-rn1dow26	307	20	usually	usually	RB
cord-277909-rn1dow26	307	21	associated	associate	VBN
cord-277909-rn1dow26	307	22	with	with	IN
cord-277909-rn1dow26	307	23	a	a	DT
cord-277909-rn1dow26	307	24	positive	positive	JJ
cord-277909-rn1dow26	307	25	sample	sample	NN
cord-277909-rn1dow26	307	26	.	.	.
cord-277909-rn1dow26	308	1	are	be	VBP
cord-277909-rn1dow26	308	2	not	not	RB
cord-277909-rn1dow26	308	3	in	in	IN
cord-277909-rn1dow26	308	4	keeping	keep	VBG
cord-277909-rn1dow26	308	5	with	with	IN
cord-277909-rn1dow26	308	6	accepted	accepted	JJ
cord-277909-rn1dow26	308	7	knowledge	knowledge	NN
cord-277909-rn1dow26	308	8	.	.	.
cord-277909-rn1dow26	309	1	For	for	IN
cord-277909-rn1dow26	309	2	example	example	NN
cord-277909-rn1dow26	309	3	,	,	,
cord-277909-rn1dow26	309	4	herpes	herpes	NN
cord-277909-rn1dow26	309	5	viruses	virus	NNS
cord-277909-rn1dow26	309	6	in	in	IN
cord-277909-rn1dow26	309	7	throat	throat	NN
cord-277909-rn1dow26	309	8	swabs	swab	NNS
cord-277909-rn1dow26	309	9	should	should	MD
cord-277909-rn1dow26	309	10	be	be	VB
cord-277909-rn1dow26	309	11	interpreted	interpret	VBN
cord-277909-rn1dow26	309	12	with	with	IN
cord-277909-rn1dow26	309	13	care	care	NN
cord-277909-rn1dow26	309	14	.	.	.
cord-277909-rn1dow26	310	1	We	-PRON-	PRP
cord-277909-rn1dow26	310	2	often	often	RB
cord-277909-rn1dow26	310	3	detect	detect	VBP
cord-277909-rn1dow26	310	4	low	low	JJ
cord-277909-rn1dow26	310	5	positive	positive	JJ
cord-277909-rn1dow26	310	6	EBV	EBV	NNP
cord-277909-rn1dow26	310	7	,	,	,
cord-277909-rn1dow26	310	8	HHV-6	HHV-6	NNP
cord-277909-rn1dow26	310	9	,	,	,
cord-277909-rn1dow26	310	10	HSV	HSV	NNP
cord-277909-rn1dow26	310	11	or	or	CC
cord-277909-rn1dow26	310	12	CMV	CMV	NNP
cord-277909-rn1dow26	310	13	in	in	IN
cord-277909-rn1dow26	310	14	throat	throat	NN
cord-277909-rn1dow26	310	15	swabs	swab	NNS
cord-277909-rn1dow26	310	16	in	in	IN
cord-277909-rn1dow26	310	17	cases	case	NNS
cord-277909-rn1dow26	310	18	of	of	IN
cord-277909-rn1dow26	310	19	respiratory	respiratory	JJ
cord-277909-rn1dow26	310	20	infection	infection	NN
cord-277909-rn1dow26	310	21	.	.	.
cord-277909-rn1dow26	311	1	Whether	whether	IN
cord-277909-rn1dow26	311	2	these	these	DT
cord-277909-rn1dow26	311	3	are	be	VBP
cord-277909-rn1dow26	311	4	the	the	DT
cord-277909-rn1dow26	311	5	cause	cause	NN
cord-277909-rn1dow26	311	6	of	of	IN
cord-277909-rn1dow26	311	7	disease	disease	NN
cord-277909-rn1dow26	311	8	or	or	CC
cord-277909-rn1dow26	311	9	unrelated	unrelated	JJ
cord-277909-rn1dow26	311	10	re	re	NNS
cord-277909-rn1dow26	311	11	-	-	NNS
cord-277909-rn1dow26	311	12	activations	activation	NNS
cord-277909-rn1dow26	311	13	are	be	VBP
cord-277909-rn1dow26	311	14	unclear	unclear	JJ
cord-277909-rn1dow26	311	15	.	.	.
cord-277909-rn1dow26	312	1	Although	although	IN
cord-277909-rn1dow26	312	2	these	these	DT
cord-277909-rn1dow26	312	3	findings	finding	NNS
cord-277909-rn1dow26	312	4	may	may	MD
cord-277909-rn1dow26	312	5	be	be	VB
cord-277909-rn1dow26	312	6	irrelevant	irrelevant	JJ
cord-277909-rn1dow26	312	7	to	to	IN
cord-277909-rn1dow26	312	8	the	the	DT
cord-277909-rn1dow26	312	9	clinical	clinical	JJ
cord-277909-rn1dow26	312	10	illness	illness	NN
cord-277909-rn1dow26	312	11	it	-PRON-	PRP
cord-277909-rn1dow26	312	12	is	be	VBZ
cord-277909-rn1dow26	312	13	important	important	JJ
cord-277909-rn1dow26	312	14	that	that	IN
cord-277909-rn1dow26	312	15	these	these	DT
cord-277909-rn1dow26	312	16	results	result	NNS
cord-277909-rn1dow26	312	17	are	be	VBP
cord-277909-rn1dow26	312	18	not	not	RB
cord-277909-rn1dow26	312	19	ignored	ignore	VBN
cord-277909-rn1dow26	312	20	.	.	.
cord-277909-rn1dow26	313	1	For	for	IN
cord-277909-rn1dow26	313	2	as	as	IN
cord-277909-rn1dow26	313	3	we	-PRON-	PRP
cord-277909-rn1dow26	313	4	gain	gain	VBP
cord-277909-rn1dow26	313	5	more	more	JJR
cord-277909-rn1dow26	313	6	experience	experience	NN
cord-277909-rn1dow26	313	7	with	with	IN
cord-277909-rn1dow26	313	8	these	these	DT
cord-277909-rn1dow26	313	9	sensitive	sensitive	JJ
cord-277909-rn1dow26	313	10	assays	assay	NNS
cord-277909-rn1dow26	313	11	we	-PRON-	PRP
cord-277909-rn1dow26	313	12	may	may	MD
cord-277909-rn1dow26	313	13	identify	identify	VB
cord-277909-rn1dow26	313	14	new	new	JJ
cord-277909-rn1dow26	313	15	,	,	,
cord-277909-rn1dow26	313	16	previously	previously	RB
cord-277909-rn1dow26	313	17	unrecognised	unrecognised	JJ
cord-277909-rn1dow26	313	18	syndromes	syndrome	NNS
cord-277909-rn1dow26	313	19	attributed	attribute	VBN
cord-277909-rn1dow26	313	20	to	to	IN
cord-277909-rn1dow26	313	21	particular	particular	JJ
cord-277909-rn1dow26	313	22	viral	viral	JJ
cord-277909-rn1dow26	313	23	pathogens	pathogen	NNS
cord-277909-rn1dow26	313	24	.	.	.
cord-277909-rn1dow26	314	1	There	there	EX
cord-277909-rn1dow26	314	2	is	be	VBZ
cord-277909-rn1dow26	314	3	no	no	DT
cord-277909-rn1dow26	314	4	doubt	doubt	NN
cord-277909-rn1dow26	314	5	that	that	IN
cord-277909-rn1dow26	314	6	in	in	IN
cord-277909-rn1dow26	314	7	the	the	DT
cord-277909-rn1dow26	314	8	coming	come	VBG
cord-277909-rn1dow26	314	9	years	year	NNS
cord-277909-rn1dow26	314	10	an	an	DT
cord-277909-rn1dow26	314	11	increasing	increase	VBG
cord-277909-rn1dow26	314	12	number	number	NN
cord-277909-rn1dow26	314	13	of	of	IN
cord-277909-rn1dow26	314	14	virology	virology	NN
cord-277909-rn1dow26	314	15	laboratories	laboratory	NNS
cord-277909-rn1dow26	314	16	will	will	MD
cord-277909-rn1dow26	314	17	utilise	utilise	VB
cord-277909-rn1dow26	314	18	real	real	JJ
cord-277909-rn1dow26	314	19	time	time	NN
cord-277909-rn1dow26	315	1	Fig	Fig	NNP
cord-277909-rn1dow26	315	2	.	.	.
cord-277909-rn1dow26	316	1	10	10	CD
cord-277909-rn1dow26	316	2	.	.	.
cord-277909-rn1dow26	317	1	The	the	DT
cord-277909-rn1dow26	317	2	turn	turn	NN
cord-277909-rn1dow26	317	3	around	around	IN
cord-277909-rn1dow26	317	4	time	time	NN
cord-277909-rn1dow26	317	5	of	of	IN
cord-277909-rn1dow26	317	6	respiratory	respiratory	JJ
cord-277909-rn1dow26	317	7	samples	sample	NNS
cord-277909-rn1dow26	317	8	in	in	IN
cord-277909-rn1dow26	317	9	2002	2002	CD
cord-277909-rn1dow26	317	10	-	-	SYM
cord-277909-rn1dow26	317	11	2003	2003	CD
cord-277909-rn1dow26	317	12	to	to	IN
cord-277909-rn1dow26	317	13	2004	2004	CD
cord-277909-rn1dow26	317	14	-	-	SYM
cord-277909-rn1dow26	317	15	2005	2005	CD
cord-277909-rn1dow26	317	16	.	.	.
cord-277909-rn1dow26	318	1	PCR	PCR	NNP
cord-277909-rn1dow26	318	2	assays	assay	NNS
cord-277909-rn1dow26	318	3	.	.	.
cord-277909-rn1dow26	319	1	As	as	IN
cord-277909-rn1dow26	319	2	a	a	DT
cord-277909-rn1dow26	319	3	result	result	NN
cord-277909-rn1dow26	319	4	virology	virology	NN
cord-277909-rn1dow26	319	5	laboratories	laboratory	NNS
cord-277909-rn1dow26	319	6	will	will	MD
cord-277909-rn1dow26	319	7	be	be	VB
cord-277909-rn1dow26	319	8	able	able	JJ
cord-277909-rn1dow26	319	9	to	to	TO
cord-277909-rn1dow26	319	10	offer	offer	VB
cord-277909-rn1dow26	319	11	more	more	JJR
cord-277909-rn1dow26	319	12	tests	test	NNS
cord-277909-rn1dow26	319	13	and	and	CC
cord-277909-rn1dow26	319	14	process	process	VBP
cord-277909-rn1dow26	319	15	more	more	JJR
cord-277909-rn1dow26	319	16	samples	sample	NNS
cord-277909-rn1dow26	319	17	while	while	IN
cord-277909-rn1dow26	319	18	reducing	reduce	VBG
cord-277909-rn1dow26	319	19	turn	turn	NN
cord-277909-rn1dow26	319	20	around	around	IN
cord-277909-rn1dow26	319	21	times	time	NNS
cord-277909-rn1dow26	319	22	.	.	.
cord-277909-rn1dow26	320	1	This	this	DT
cord-277909-rn1dow26	320	2	can	can	MD
cord-277909-rn1dow26	320	3	be	be	VB
cord-277909-rn1dow26	320	4	highlighted	highlight	VBN
cord-277909-rn1dow26	320	5	by	by	IN
cord-277909-rn1dow26	320	6	our	-PRON-	PRP$
cord-277909-rn1dow26	320	7	winter	winter	NN
cord-277909-rn1dow26	320	8	respiratory	respiratory	JJ
cord-277909-rn1dow26	320	9	surveillance	surveillance	NN
cord-277909-rn1dow26	320	10	service	service	NN
cord-277909-rn1dow26	320	11	(	(	-LRB-
cord-277909-rn1dow26	320	12	SERVIS	SERVIS	NNP
cord-277909-rn1dow26	320	13	)	)	-RRB-
cord-277909-rn1dow26	320	14	,	,	,
cord-277909-rn1dow26	320	15	which	which	WDT
cord-277909-rn1dow26	320	16	currently	currently	RB
cord-277909-rn1dow26	320	17	tests	test	VBZ
cord-277909-rn1dow26	320	18	for	for	IN
cord-277909-rn1dow26	320	19	12	12	CD
cord-277909-rn1dow26	320	20	pathogens	pathogen	NNS
cord-277909-rn1dow26	320	21	.	.	.
cord-277909-rn1dow26	321	1	During	during	IN
cord-277909-rn1dow26	321	2	the	the	DT
cord-277909-rn1dow26	321	3	2004	2004	CD
cord-277909-rn1dow26	321	4	-	-	SYM
cord-277909-rn1dow26	321	5	2005	2005	CD
cord-277909-rn1dow26	321	6	season	season	NN
cord-277909-rn1dow26	321	7	509	509	CD
cord-277909-rn1dow26	321	8	samples	sample	NNS
cord-277909-rn1dow26	321	9	were	be	VBD
cord-277909-rn1dow26	321	10	tested	test	VBN
cord-277909-rn1dow26	321	11	with	with	IN
cord-277909-rn1dow26	321	12	80	80	CD
cord-277909-rn1dow26	321	13	%	%	NN
cord-277909-rn1dow26	321	14	of	of	IN
cord-277909-rn1dow26	321	15	results	result	NNS
cord-277909-rn1dow26	321	16	reported	report	VBN
cord-277909-rn1dow26	321	17	within	within	IN
cord-277909-rn1dow26	321	18	3	3	CD
cord-277909-rn1dow26	321	19	days	day	NNS
cord-277909-rn1dow26	321	20	of	of	IN
cord-277909-rn1dow26	321	21	the	the	DT
cord-277909-rn1dow26	321	22	samples	sample	NNS
cord-277909-rn1dow26	321	23	arriving	arrive	VBG
cord-277909-rn1dow26	321	24	in	in	IN
cord-277909-rn1dow26	321	25	the	the	DT
cord-277909-rn1dow26	321	26	laboratory	laboratory	NN
cord-277909-rn1dow26	321	27	(	(	-LRB-
cord-277909-rn1dow26	321	28	Fig	Fig	NNP
cord-277909-rn1dow26	321	29	.	.	.
cord-277909-rn1dow26	321	30	10	10	CD
cord-277909-rn1dow26	321	31	)	)	-RRB-
cord-277909-rn1dow26	321	32	.	.	.
cord-277909-rn1dow26	322	1	For	for	IN
cord-277909-rn1dow26	322	2	2002	2002	CD
cord-277909-rn1dow26	322	3	-	-	SYM
cord-277909-rn1dow26	322	4	2003	2003	CD
cord-277909-rn1dow26	322	5	when	when	WRB
cord-277909-rn1dow26	322	6	gel	gel	NN
cord-277909-rn1dow26	322	7	based	base	VBN
cord-277909-rn1dow26	322	8	PCR	PCR	NNP
cord-277909-rn1dow26	322	9	was	be	VBD
cord-277909-rn1dow26	322	10	used	use	VBN
cord-277909-rn1dow26	322	11	to	to	TO
cord-277909-rn1dow26	322	12	detect	detect	VB
cord-277909-rn1dow26	322	13	Influenza	Influenza	NNP
cord-277909-rn1dow26	322	14	A	A	NNP
cord-277909-rn1dow26	322	15	and	and	CC
cord-277909-rn1dow26	322	16	B	b	NN
cord-277909-rn1dow26	322	17	,	,	,
cord-277909-rn1dow26	322	18	RSV	RSV	NNP
cord-277909-rn1dow26	322	19	and	and	CC
cord-277909-rn1dow26	322	20	picornavirus	picornavirus	NNP
cord-277909-rn1dow26	322	21	,	,	,
cord-277909-rn1dow26	322	22	554	554	CD
cord-277909-rn1dow26	322	23	samples	sample	NNS
cord-277909-rn1dow26	322	24	were	be	VBD
cord-277909-rn1dow26	322	25	tested	test	VBN
cord-277909-rn1dow26	322	26	in	in	IN
cord-277909-rn1dow26	322	27	total	total	NN
cord-277909-rn1dow26	322	28	.	.	.
cord-277909-rn1dow26	323	1	Only	only	RB
cord-277909-rn1dow26	323	2	3.6	3.6	CD
cord-277909-rn1dow26	323	3	%	%	NN
cord-277909-rn1dow26	323	4	of	of	IN
cord-277909-rn1dow26	323	5	results	result	NNS
cord-277909-rn1dow26	323	6	were	be	VBD
cord-277909-rn1dow26	323	7	available	available	JJ
cord-277909-rn1dow26	323	8	in	in	IN
cord-277909-rn1dow26	323	9	3	3	CD
cord-277909-rn1dow26	323	10	days	day	NNS
cord-277909-rn1dow26	323	11	with	with	IN
cord-277909-rn1dow26	323	12	most	most	JJS
cord-277909-rn1dow26	323	13	results	result	NNS
cord-277909-rn1dow26	323	14	returned	return	VBN
cord-277909-rn1dow26	323	15	to	to	IN
cord-277909-rn1dow26	323	16	users	user	NNS
cord-277909-rn1dow26	323	17	within	within	IN
cord-277909-rn1dow26	323	18	14	14	CD
cord-277909-rn1dow26	323	19	days	day	NNS
cord-277909-rn1dow26	323	20	.	.	.
cord-277909-rn1dow26	324	1	With	with	IN
cord-277909-rn1dow26	324	2	a	a	DT
cord-277909-rn1dow26	324	3	slightly	slightly	RB
cord-277909-rn1dow26	324	4	extended	extended	JJ
cord-277909-rn1dow26	324	5	working	working	NN
cord-277909-rn1dow26	324	6	day	day	NN
cord-277909-rn1dow26	324	7	,	,	,
cord-277909-rn1dow26	324	8	real	real	JJ
cord-277909-rn1dow26	324	9	time	time	NN
cord-277909-rn1dow26	324	10	PCR	PCR	NNP
cord-277909-rn1dow26	324	11	results	result	NNS
cord-277909-rn1dow26	324	12	ought	ought	MD
cord-277909-rn1dow26	324	13	to	to	TO
cord-277909-rn1dow26	324	14	be	be	VB
cord-277909-rn1dow26	324	15	reported	report	VBN
cord-277909-rn1dow26	324	16	within	within	IN
cord-277909-rn1dow26	324	17	36	36	CD
cord-277909-rn1dow26	324	18	h	h	NN
cord-277909-rn1dow26	324	19	of	of	IN
cord-277909-rn1dow26	324	20	receipt	receipt	NN
cord-277909-rn1dow26	324	21	.	.	.
cord-277909-rn1dow26	325	1	The	the	DT
cord-277909-rn1dow26	325	2	routine	routine	JJ
cord-277909-rn1dow26	325	3	use	use	NN
cord-277909-rn1dow26	325	4	of	of	IN
cord-277909-rn1dow26	325	5	real	real	JJ
cord-277909-rn1dow26	325	6	time	time	NN
cord-277909-rn1dow26	325	7	PCR	PCR	NNP
cord-277909-rn1dow26	325	8	will	will	MD
cord-277909-rn1dow26	325	9	have	have	VB
cord-277909-rn1dow26	325	10	several	several	JJ
cord-277909-rn1dow26	325	11	benefits	benefit	NNS
cord-277909-rn1dow26	325	12	.	.	.
cord-277909-rn1dow26	326	1	First	first	RB
cord-277909-rn1dow26	326	2	it	-PRON-	PRP
cord-277909-rn1dow26	326	3	will	will	MD
cord-277909-rn1dow26	326	4	aid	aid	VB
cord-277909-rn1dow26	326	5	patient	patient	JJ
cord-277909-rn1dow26	326	6	management	management	NN
cord-277909-rn1dow26	326	7	(	(	-LRB-
cord-277909-rn1dow26	326	8	prognosis	prognosis	NN
cord-277909-rn1dow26	326	9	,	,	,
cord-277909-rn1dow26	326	10	treatment	treatment	NN
cord-277909-rn1dow26	326	11	guidance	guidance	NN
cord-277909-rn1dow26	326	12	and	and	CC
cord-277909-rn1dow26	326	13	infection	infection	NN
cord-277909-rn1dow26	326	14	control	control	NN
cord-277909-rn1dow26	326	15	)	)	-RRB-
cord-277909-rn1dow26	326	16	and	and	CC
cord-277909-rn1dow26	326	17	may	may	MD
cord-277909-rn1dow26	326	18	assist	assist	VB
cord-277909-rn1dow26	326	19	in	in	IN
cord-277909-rn1dow26	326	20	the	the	DT
cord-277909-rn1dow26	326	21	development	development	NN
cord-277909-rn1dow26	326	22	of	of	IN
cord-277909-rn1dow26	326	23	new	new	JJ
cord-277909-rn1dow26	326	24	antiviral	antiviral	JJ
cord-277909-rn1dow26	326	25	therapies	therapy	NNS
cord-277909-rn1dow26	326	26	.	.	.
cord-277909-rn1dow26	327	1	Real	real	JJ
cord-277909-rn1dow26	327	2	time	time	NN
cord-277909-rn1dow26	327	3	PCR	PCR	NNP
cord-277909-rn1dow26	327	4	will	will	MD
cord-277909-rn1dow26	327	5	also	also	RB
cord-277909-rn1dow26	327	6	improve	improve	VB
cord-277909-rn1dow26	327	7	the	the	DT
cord-277909-rn1dow26	327	8	sensitivity	sensitivity	NN
cord-277909-rn1dow26	327	9	of	of	IN
cord-277909-rn1dow26	327	10	the	the	DT
cord-277909-rn1dow26	327	11	surveillance	surveillance	NN
cord-277909-rn1dow26	327	12	of	of	IN
cord-277909-rn1dow26	327	13	viral	viral	JJ
cord-277909-rn1dow26	327	14	pathogens	pathogen	NNS
cord-277909-rn1dow26	327	15	,	,	,
cord-277909-rn1dow26	327	16	increasing	increase	VBG
cord-277909-rn1dow26	327	17	our	-PRON-	PRP$
cord-277909-rn1dow26	327	18	understanding	understanding	NN
cord-277909-rn1dow26	327	19	of	of	IN
cord-277909-rn1dow26	327	20	these	these	DT
cord-277909-rn1dow26	327	21	important	important	JJ
cord-277909-rn1dow26	327	22	infections	infection	NNS
cord-277909-rn1dow26	327	23	,	,	,
cord-277909-rn1dow26	327	24	providing	provide	VBG
cord-277909-rn1dow26	327	25	accurate	accurate	JJ
cord-277909-rn1dow26	327	26	assessments	assessment	NNS
cord-277909-rn1dow26	327	27	of	of	IN
cord-277909-rn1dow26	327	28	the	the	DT
cord-277909-rn1dow26	327	29	morbidity	morbidity	NN
cord-277909-rn1dow26	327	30	and	and	CC
cord-277909-rn1dow26	327	31	economic	economic	JJ
cord-277909-rn1dow26	327	32	cost	cost	NN
cord-277909-rn1dow26	327	33	of	of	IN
cord-277909-rn1dow26	327	34	disease	disease	NN
cord-277909-rn1dow26	327	35	and	and	CC
cord-277909-rn1dow26	327	36	facilitating	facilitate	VBG
cord-277909-rn1dow26	327	37	the	the	DT
cord-277909-rn1dow26	327	38	implementation	implementation	NN
cord-277909-rn1dow26	327	39	of	of	IN
cord-277909-rn1dow26	327	40	public	public	JJ
cord-277909-rn1dow26	327	41	health	health	NN
cord-277909-rn1dow26	327	42	prevention	prevention	NN
cord-277909-rn1dow26	327	43	measures	measure	NNS
cord-277909-rn1dow26	327	44	.	.	.
cord-277909-rn1dow26	328	1	356	356	CD
cord-277909-rn1dow26	328	2	2.1	2.1	CD
cord-277909-rn1dow26	328	3	.	.	.
cord-277909-rn1dow26	329	1	Which	which	WDT
cord-277909-rn1dow26	329	2	real	real	JJ
cord-277909-rn1dow26	329	3	time	time	NN
cord-277909-rn1dow26	329	4	PCR	PCR	NNP
cord-277909-rn1dow26	329	5	chemistry	chemistry	NN
cord-277909-rn1dow26	329	6	is	be	VBZ
cord-277909-rn1dow26	329	7	best	good	JJS
cord-277909-rn1dow26	329	8	for	for	IN
cord-277909-rn1dow26	329	9	viral	viral	JJ
cord-277909-rn1dow26	329	10	diagnosis	diagnosis	NN
cord-277909-rn1dow26	329	11	?	?	.
cord-277909-rn1dow26	330	1	Automated	automate	VBN
cord-277909-rn1dow26	330	2	extraction	extraction	NN
cord-277909-rn1dow26	330	3	and	and	CC
cord-277909-rn1dow26	330	4	liquid	liquid	NN
cord-277909-rn1dow26	330	5	handling	handling	NN
cord-277909-rn1dow26	330	6	equipment	equipment	NN
cord-277909-rn1dow26	331	1	Continual	continual	JJ
cord-277909-rn1dow26	331	2	assessment	assessment	NN
cord-277909-rn1dow26	331	3	of	of	IN
cord-277909-rn1dow26	331	4	the	the	DT
cord-277909-rn1dow26	331	5	sensitivity	sensitivity	NN
cord-277909-rn1dow26	331	6	of	of	IN
cord-277909-rn1dow26	331	7	real	real	JJ
cord-277909-rn1dow26	331	8	time	time	NN
cord-277909-rn1dow26	331	9	PCR	PCR	NNP
cord-277909-rn1dow26	331	10	primers	primer	NNS
cord-277909-rn1dow26	331	11	and	and	CC
cord-277909-rn1dow26	331	12	probe	probe	VB
cord-277909-rn1dow26	331	13	Basic	basic	JJ
cord-277909-rn1dow26	331	14	principles	principle	NNS
cord-277909-rn1dow26	331	15	of	of	IN
cord-277909-rn1dow26	331	16	real	real	JJ
cord-277909-rn1dow26	331	17	time	time	NN
cord-277909-rn1dow26	331	18	quantitative	quantitative	JJ
cord-277909-rn1dow26	331	19	PCR	pcr	NN
cord-277909-rn1dow26	331	20	Preventing	prevent	VBG
cord-277909-rn1dow26	331	21	PCR	PCR	NNP
cord-277909-rn1dow26	331	22	amplification	amplification	NN
cord-277909-rn1dow26	331	23	carryover	carryover	NN
cord-277909-rn1dow26	331	24	contamination	contamination	NN
cord-277909-rn1dow26	331	25	in	in	IN
cord-277909-rn1dow26	331	26	a	a	DT
cord-277909-rn1dow26	331	27	clinical	clinical	JJ
cord-277909-rn1dow26	331	28	laboratory	laboratory	NN
cord-277909-rn1dow26	331	29	Pitfalls	pitfall	NNS
cord-277909-rn1dow26	331	30	of	of	IN
cord-277909-rn1dow26	331	31	quantitative	quantitative	JJ
cord-277909-rn1dow26	331	32	real	real	JJ
cord-277909-rn1dow26	331	33	time	time	NN
cord-277909-rn1dow26	331	34	reverse	reverse	NN
cord-277909-rn1dow26	331	35	-	-	HYPH
cord-277909-rn1dow26	331	36	transcription	transcription	NN
cord-277909-rn1dow26	331	37	polymerase	polymerase	NN
cord-277909-rn1dow26	331	38	chain	chain	NN
cord-277909-rn1dow26	331	39	reaction	reaction	NN
cord-277909-rn1dow26	331	40	A	a	DT
cord-277909-rn1dow26	331	41	coloured	coloured	JJ
cord-277909-rn1dow26	331	42	version	version	NN
cord-277909-rn1dow26	331	43	of	of	IN
cord-277909-rn1dow26	331	44	the	the	DT
cord-277909-rn1dow26	331	45	J	J	NNP
cord-277909-rn1dow26	331	46	chart	chart	NN
cord-277909-rn1dow26	331	47	or	or	CC
cord-277909-rn1dow26	331	48	the	the	DT
cord-277909-rn1dow26	331	49	amc	amc	NNP
cord-277909-rn1dow26	331	50	-	-	HYPH
cord-277909-rn1dow26	331	51	D	D	NNP
cord-277909-rn1dow26	331	52	J	J	NNP
cord-277909-rn1dow26	331	53	-	-	HYPH
cord-277909-rn1dow26	331	54	Chart	Chart	NNP
cord-277909-rn1dow26	331	55	Molecular	molecular	JJ
cord-277909-rn1dow26	331	56	techniques	technique	NNS
cord-277909-rn1dow26	331	57	for	for	IN
cord-277909-rn1dow26	331	58	detection	detection	NN
cord-277909-rn1dow26	331	59	,	,	,
cord-277909-rn1dow26	331	60	identification	identification	NN
cord-277909-rn1dow26	331	61	and	and	CC
cord-277909-rn1dow26	331	62	analysis	analysis	NN
cord-277909-rn1dow26	331	63	of	of	IN
cord-277909-rn1dow26	331	64	human	human	JJ
cord-277909-rn1dow26	331	65	papillomaviruses	papillomaviruse	NNS
cord-277909-rn1dow26	331	66	(	(	-LRB-
cord-277909-rn1dow26	331	67	HPVs	HPVs	NNP
cord-277909-rn1dow26	331	68	)	)	-RRB-
cord-277909-rn1dow26	332	1	My	-PRON-	PRP$
cord-277909-rn1dow26	332	2	favourite	favourite	JJ
cord-277909-rn1dow26	332	3	primers	primer	NNS
cord-277909-rn1dow26	332	4	:	:	:
cord-277909-rn1dow26	332	5	real	real	JJ
cord-277909-rn1dow26	332	6	time	time	NN
cord-277909-rn1dow26	332	7	RT	rt	NN
cord-277909-rn1dow26	332	8	-	-	HYPH
cord-277909-rn1dow26	332	9	PCR	PCR	NNP
cord-277909-rn1dow26	332	10	detection	detection	NN
cord-277909-rn1dow26	332	11	of	of	IN
cord-277909-rn1dow26	332	12	12	12	CD
cord-277909-rn1dow26	332	13	respiratory	respiratory	JJ
cord-277909-rn1dow26	332	14	viral	viral	JJ
cord-277909-rn1dow26	332	15	infections	infection	NNS
cord-277909-rn1dow26	332	16	in	in	IN
cord-277909-rn1dow26	332	17	four	four	CD
cord-277909-rn1dow26	332	18	triplex	triplex	NN
cord-277909-rn1dow26	332	19	reactions	reaction	NNS
cord-277909-rn1dow26	332	20	Optimisation	Optimisation	NNP
cord-277909-rn1dow26	332	21	of	of	IN
cord-277909-rn1dow26	332	22	PCR	PCR	NNP
cord-277909-rn1dow26	332	23	reactions	reaction	NNS
cord-277909-rn1dow26	332	24	using	use	VBG
cord-277909-rn1dow26	332	25	primer	primer	NN
cord-277909-rn1dow26	332	26	chessboarding	chessboarding	NNP
cord-277909-rn1dow26	332	27	Evaluation	Evaluation	NNP
cord-277909-rn1dow26	332	28	of	of	IN
cord-277909-rn1dow26	332	29	a	a	DT
cord-277909-rn1dow26	332	30	multiplex	multiplex	JJ
cord-277909-rn1dow26	332	31	real	real	JJ
cord-277909-rn1dow26	332	32	time	time	NN
cord-277909-rn1dow26	332	33	reverse	reverse	NN
cord-277909-rn1dow26	332	34	transcriptase	transcriptase	NN
cord-277909-rn1dow26	332	35	PCR	pcr	NN
cord-277909-rn1dow26	332	36	assay	assay	NN
cord-277909-rn1dow26	332	37	for	for	IN
cord-277909-rn1dow26	332	38	detection	detection	NN
cord-277909-rn1dow26	332	39	and	and	CC
cord-277909-rn1dow26	332	40	differentiation	differentiation	NN
cord-277909-rn1dow26	332	41	of	of	IN
cord-277909-rn1dow26	332	42	influenza	influenza	NN
cord-277909-rn1dow26	332	43	viruses	virus	NNS
cord-277909-rn1dow26	332	44	A	A	NNP
cord-277909-rn1dow26	332	45	and	and	CC
cord-277909-rn1dow26	332	46	B	b	NN
cord-277909-rn1dow26	332	47	during	during	IN
cord-277909-rn1dow26	332	48	the	the	DT
cord-277909-rn1dow26	332	49	2001	2001	CD
cord-277909-rn1dow26	332	50	-	-	SYM
cord-277909-rn1dow26	332	51	2002	2002	CD
cord-277909-rn1dow26	332	52	influenza	influenza	NN
cord-277909-rn1dow26	332	53	season	season	NN
cord-277909-rn1dow26	332	54	in	in	IN
cord-277909-rn1dow26	332	55	Israel	Israel	NNP
cord-277909-rn1dow26	332	56	Allelic	Allelic	NNP
cord-277909-rn1dow26	332	57	discrimination	discrimination	NN
cord-277909-rn1dow26	332	58	by	by	IN
cord-277909-rn1dow26	332	59	nick	nick	NNP
cord-277909-rn1dow26	332	60	-	-	HYPH
cord-277909-rn1dow26	332	61	translation	translation	NN
cord-277909-rn1dow26	332	62	PCR	PCR	NNP
cord-277909-rn1dow26	332	63	with	with	IN
cord-277909-rn1dow26	332	64	fluorogenic	fluorogenic	JJ
cord-277909-rn1dow26	332	65	probes	probe	NNS
cord-277909-rn1dow26	332	66	Real	real	JJ
cord-277909-rn1dow26	332	67	time	time	NN
cord-277909-rn1dow26	332	68	PCR	PCR	NNP
cord-277909-rn1dow26	332	69	in	in	IN
cord-277909-rn1dow26	332	70	the	the	DT
cord-277909-rn1dow26	332	71	microbiology	microbiology	NN
cord-277909-rn1dow26	332	72	laboratory	laboratory	NN
cord-277909-rn1dow26	333	1	Genogroup	Genogroup	NNP
cord-277909-rn1dow26	333	2	I	I	NNP
cord-277909-rn1dow26	333	3	and	and	CC
cord-277909-rn1dow26	333	4	II	II	NNP
cord-277909-rn1dow26	333	5	noroviruses	noroviruses	NNP
cord-277909-rn1dow26	333	6	detected	detect	VBD
cord-277909-rn1dow26	333	7	in	in	IN
cord-277909-rn1dow26	333	8	stool	stool	NN
cord-277909-rn1dow26	333	9	samples	sample	NNS
cord-277909-rn1dow26	333	10	by	by	IN
cord-277909-rn1dow26	333	11	real	real	JJ
cord-277909-rn1dow26	333	12	time	time	NN
cord-277909-rn1dow26	333	13	reverse	reverse	NN
cord-277909-rn1dow26	333	14	transcription	transcription	NN
cord-277909-rn1dow26	333	15	-	-	HYPH
cord-277909-rn1dow26	333	16	PCR	pcr	NN
cord-277909-rn1dow26	333	17	using	use	VBG
cord-277909-rn1dow26	333	18	highly	highly	RB
cord-277909-rn1dow26	333	19	degenerate	degenerate	JJ
cord-277909-rn1dow26	333	20	universal	universal	JJ
cord-277909-rn1dow26	333	21	primers	primer	NNS
cord-277909-rn1dow26	333	22	Diagnostic	diagnostic	JJ
cord-277909-rn1dow26	333	23	value	value	NN
cord-277909-rn1dow26	333	24	of	of	IN
cord-277909-rn1dow26	333	25	real	real	JJ
cord-277909-rn1dow26	333	26	time	time	NN
cord-277909-rn1dow26	333	27	capillary	capillary	JJ
cord-277909-rn1dow26	333	28	thermal	thermal	JJ
cord-277909-rn1dow26	333	29	cycler	cycler	NN
cord-277909-rn1dow26	333	30	in	in	IN
cord-277909-rn1dow26	333	31	virus	virus	NN
cord-277909-rn1dow26	333	32	detection	detection	NN
cord-277909-rn1dow26	333	33	Rapid	Rapid	NNP
cord-277909-rn1dow26	333	34	and	and	CC
cord-277909-rn1dow26	333	35	sensitive	sensitive	JJ
cord-277909-rn1dow26	333	36	method	method	NN
cord-277909-rn1dow26	333	37	using	use	VBG
cord-277909-rn1dow26	333	38	multiplex	multiplex	JJ
cord-277909-rn1dow26	333	39	real	real	JJ
cord-277909-rn1dow26	333	40	time	time	NN
cord-277909-rn1dow26	333	41	PCR	PCR	NNP
cord-277909-rn1dow26	333	42	for	for	IN
cord-277909-rn1dow26	333	43	diagnosis	diagnosis	NN
cord-277909-rn1dow26	333	44	of	of	IN
cord-277909-rn1dow26	333	45	infections	infection	NNS
cord-277909-rn1dow26	333	46	by	by	IN
cord-277909-rn1dow26	333	47	influenza	influenza	NN
cord-277909-rn1dow26	333	48	a	a	NN
cord-277909-rn1dow26	333	49	and	and	CC
cord-277909-rn1dow26	333	50	influenza	influenza	NN
cord-277909-rn1dow26	333	51	B	b	NN
cord-277909-rn1dow26	333	52	viruses	virus	NNS
cord-277909-rn1dow26	333	53	,	,	,
cord-277909-rn1dow26	333	54	respiratory	respiratory	JJ
cord-277909-rn1dow26	333	55	syncytial	syncytial	JJ
cord-277909-rn1dow26	333	56	virus	virus	NN
cord-277909-rn1dow26	333	57	,	,	,
cord-277909-rn1dow26	333	58	and	and	CC
cord-277909-rn1dow26	333	59	parainfluenza	parainfluenza	NNP
cord-277909-rn1dow26	333	60	viruses	virus	NNS
cord-277909-rn1dow26	333	61	1	1	CD
cord-277909-rn1dow26	333	62	,	,	,
cord-277909-rn1dow26	333	63	2	2	CD
cord-277909-rn1dow26	333	64	,	,	,
cord-277909-rn1dow26	333	65	3	3	CD
cord-277909-rn1dow26	333	66	and	and	CC
cord-277909-rn1dow26	333	67	4	4	CD
cord-277909-rn1dow26	333	68	Molecular	molecular	JJ
cord-277909-rn1dow26	333	69	beacons	beacon	NNS
cord-277909-rn1dow26	333	70	:	:	:
cord-277909-rn1dow26	334	1	probes	probe	NNS
cord-277909-rn1dow26	334	2	that	that	WDT
cord-277909-rn1dow26	334	3	fluoresce	fluoresce	VBP
cord-277909-rn1dow26	334	4	upon	upon	IN
cord-277909-rn1dow26	334	5	hybridization	hybridization	NN