id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-353640-giznbcpd	Barza, Ruby	Use of a Simplified Sample Processing step without RNA Extraction for direct SARS-CoV-2 RT-PCR Detection	2020-08-11		.txt	text/plain	1204	59	55	RT-PCR results using the ChromaCode HDPCR™ SARS-CoV-2 were compared using the heat-RNA release method and an automated RNA extraction system (EMAG). We compared the diagnostic sensitivity of the heat-RNA release method for detection of SARS-CoV-2 using NP swabs in viral transport media (VTM) to results obtained using an automated RNA extraction system (EMAG ® , bioMerieux, Durham, NC). A total of 174 COVID-19 positive samples were used for the study comprising 87 unique COVID-19 NP including Ct values using the heat-RNA release method were compared to the results obtained using the automated RNA-extraction method with both the LDT and ChromaCode SARS-CoV-2 assays. Comparison of the heat-RNA release method to EMAG ® using the ChromaCode SARS-CoV-2 RUO alone yielded a 94% agreement (81/86 positive samples). We found that the direct detection of SARS-CoV-2 using a 65°C heat inactivation and release step for 20minutes without RNA extraction and purification performed very well compared to the use of an automated RNA extraction instrument.	./cache/cord-353640-giznbcpd.txt	./txt/cord-353640-giznbcpd.txt