id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-336237-hmczy0am	Kitagawa, Yutaro	Evaluation of rapid diagnosis of novel coronavirus disease (COVID-19) using loop-mediated isothermal amplification	2020-05-21		.txt	text/plain	1199	73	54	authors: Kitagawa, Yutaro; Orihara, Yuta; Kawamura, Rieko; Imai, Kazuo; Sakai, Jun; Tarumoto, Norihito; Matsuoka, Masaru; Takeuchi, Shinichi; Maesaki, Shigefumi; Maeda, Takuya title: Evaluation of rapid diagnosis of novel coronavirus disease (COVID-19) using loop-mediated isothermal amplification In this study, we evaluated the effectiveness of the Loopamp® 2019-SARSCoV-2 Detection Reagent Kit, which uses loop-mediated isothermal amplification (LAMP) technology. The RT-LAMP test kit amplified the targeted RNA of a SARS-CoV-2 isolate with a detection limit of 1.0 × 101 copies/μL, which was comparable to the detection sensitivity of quantitative reverse transcription PCR (RT-qPCR). To evaluate the analytical sensitivity of the RT-LAMP method, we used purified and quantified total RNA of SARS-CoV-2, which was provided by the National Institute of Infectious Diseases, Japan, as a standard specimen for the molecular diagnosis of COVID-19. Rapid and visual detection of 2019 novel coronavirus (SARS-CoV-2) by a reverse transcription loop-mediated isothermal amplification assay	./cache/cord-336237-hmczy0am.txt	./txt/cord-336237-hmczy0am.txt