id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-313749-f2ct57em	Brittain-Long, Robin	Multiplex real-time PCR for detection of respiratory tract infections	2007-12-26		.txt	text/plain	1566	94	50	title: Multiplex real-time PCR for detection of respiratory tract infections STUDY DESIGN: An assay targeting influenza virus A (IfA) and B (IfB), parainfluenza 1-3 (PIV), human metapneumovirus (MPV), respiratory syncytial virus (RSV), rhinovirus (RV), enterovirus (EV), adenovirus (AdV), human coronaviruses (229E, OC43, NL63), M. We developed a real-time PCR procedure, based on automated specimen extraction and multiplex amplification, at a relatively low cost (D 33). We believe that a combination of low cost, high accuracy and prompt result delivery is the key to achieving a wide clinical use of molecular diagnostics of respiratory infections. Further studies of respiratory infection aetiologies in different patient categories, and of the clinical utility of this and similar multiplex assays, need to be carried out. Real-time RT-PCR detection of 12 respiratory viral infections in four triplex reactions Rapid and sensitive method using multiplex real-time PCR for diagnosis of infections by influenza A and influenza B viruses, respiratory syncytial virus, and parainfluenza viruses 1, 2, 3, and 4	./cache/cord-313749-f2ct57em.txt	./txt/cord-313749-f2ct57em.txt