id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-289139-5ljqnc39	Mengelle, C.	The use of a multiplex real-time PCR assay for diagnosing acute respiratory viral infections in children attending an emergency unit	2014-09-03		.txt	text/plain	2574	146	51	title: The use of a multiplex real-time PCR assay for diagnosing acute respiratory viral infections in children attending an emergency unit BACKGROUND: The use of a multiplex molecular technique to identify the etiological pathogen of respiratory viral infections might be a support as clinical signs are not characteristic. OBJECTIVES: The aim of the study was to evaluate a multiplex molecular real-time assay for the routine diagnosis of respiratory viruses, to analyze the symptoms associated with the pathogens detected and to determine the spread of virus during the period. This assay can detect 15 viruses: influenza viruses (IV) types A and B, parainfluenza viruses 1 to 4 (PiV), respiratory syncytial viruses A and B (RSV), rhinovirus (RV), coronaviruses 229E, OC43 and NL63 (CoV), human metapneumovirus (MPV) and adenovirus (ADV). RSV and RV were the most prevalent pathogens, particularly in the youngest children, and co-infections were associated with more severe respiratory symptoms.	./cache/cord-289139-5ljqnc39.txt	./txt/cord-289139-5ljqnc39.txt