id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-277909-rn1dow26	Gunson, R.N.	Practical experience of high throughput real time PCR in the routine diagnostic virology setting	2006-02-07		.txt	text/plain	6853	342	54	In comparison to traditional gel-based PCR assays, real time PCR offers increased sensitivity and specificity in a rapid format (turn around time from sample receipt to result <5 h). Most of the published real time probe based PCR assays for viral diagnosis utilise either molecular beacons or dual labelled probes although more recent publications tend to favour the use of dual labelled probes. In real time PCR, the signal is detected early in the amplification process, and therefore the end-point variation seen in gel-based assays does not affect the result. Despite this we still perform an initial optimisation of both primer and probe concentrations to ensure we are running our real time PCR assays at their most sensitive and efficient. Some manufacturers are now producing real time reaction mixes specifically designed for use with multiplex assays, and provide guidelines on the optimal primer and probe concentrations to use.	./cache/cord-277909-rn1dow26.txt	./txt/cord-277909-rn1dow26.txt