id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-269407-6i66zf0e	Rutvisuttinunt, Wiriya	Retrospective use of next-generation sequencing reveals the presence of Enteroviruses in acute influenza-like illness respiratory samples collected in South/South-East Asia during 2010–2013	2017-07-14		.txt	text/plain	3868	196	46	Three hundred and twenty-four samples which were found to be negative for influenza virus after screening with real-time RT-PCR and cell-based culture techniques demonstrated the potential for viral infection with evident cytopathic effect (CPE) in several cell lines. OBJECTIVE: To assess whether whole genome next-generation sequencing (WG-NGS) together with conventional molecular assays can be used to reveal the etiology of influenza negative, but CPE positive specimens. Individual samples in the positive pools were confirmed by qRT-PCR, RT-PCR, PCR and Sanger sequencing from the CPE culture and original clinical specimens. The 310 culture supernatants that made up the 29 pools were further tested by pathogen-specific PCR, RT-PCR or rRT-PCR to validate the WG-NGS findings and to quantify the percentage of samples within each pool where viruses were present ( Table 2) . In addition, standard molecular procedures were conducted in selected samples and results validated the presence of the identified pathogens in both clinical specimens and CPE culture.	./cache/cord-269407-6i66zf0e.txt	./txt/cord-269407-6i66zf0e.txt