id author title date pages extension mime words sentences flesch summary cache txt cord-263118-6sf41rsj Landry, Marie L. Real-time PCR compared to Binax NOW and cytospin-immunofluorescence for detection of influenza in hospitalized patients 2008-07-18 .txt text/plain 2586 137 56 STUDY DESIGN: Binax NOW, cytospin-enhanced direct immunofluoroescence (DFA), and influenza A and B multiplex TaqMan RT-PCR were performed on 237 clinical samples. RESULTS: Binax NOW detected 70 (53.0%), cytospin-DFA detected 127 (96.2%), and TaqMan RT-PCR detected 132 (100%) influenza-positive samples. CONCLUSIONS: The accuracy of real-time RT-PCR should greatly improve the diagnosis of influenza in hospitals using simple rapid flu tests, but may have a more modest impact in hospitals with expertise in cytospin-DFA. In our hospital, cytospin-enhanced direct immunofluorescence (DFA) is performed on respiratory samples when Virology is open, and a rapid influenza test, Binax NOW, is used in the Core Laboratory when Virology is closed (Landry and Ferguson, 2000; Landry et al., 2004) . During the study period, reflex cultures were performed on 683 DFA-negative samples, but only three influenza A positives were detected. ./cache/cord-263118-6sf41rsj.txt ./txt/cord-263118-6sf41rsj.txt