id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-262045-r2iqpmmc	Smits, Saskia L.	Reliable typing of MERS-CoV variants with a small genome fragment	2014-12-15		.txt	text/plain	2151	110	49	RESULTS: A reverse-transcription PCR assay for MERS-CoV targeting a 615 bp spike fragment provides a phylogenetic clustering of MERS-CoV variants comparable to that of full-length genomes. In addition, the MERS-CoV variant typing assay was performed on camel samples from a slaughterhouse in Qatar [13] and sequences for 14 MERS-CoV positive animals with cycle threshold values ranging from 12.9 to 32.2 as determined by UpE real time RT-PCR [17, 18] were obtained (Fig. 2) . Subsequent analyses revealed a region in the open reading frame that encodes the spike protein with a number of positions in which nucleotide variation occurs between MERS-CoV variants with a strong phylogenetic signal regarding previously identified clusters of viruses based on full-length MERS-CoV genomes. Middle East respiratory syndrome coronavirus quasispecies that include homologues of human isolates revealed through whole-genome analysis and virus cultured from dromedary camels in Saudi Arabia	./cache/cord-262045-r2iqpmmc.txt	./txt/cord-262045-r2iqpmmc.txt