id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-282360-byqhzyzi	Zhang, Dingmei	Enterovirus 71 vaccine: close but still far	2010-04-18		.txt	text/plain	4404	248	43	To overcome the potential problem of reversion to virulence of attenuated strain vaccine, subunit vaccines consisting of only one or a few 'subunit' proteins of the pathogen that can stimulate immune responses directed at the intact virus have been developed using recombinant DNA technology. 21 have described a recombinant VP1 protein expressed in Escherichia coli BL21, showing that the VP1 protein with a complete adjuvant is able to elicit a neutralizing antibody response, enhance T helper cell proliferation, and induce high levels of interleukin (IL)-10 and interferon (IFN)-g in mice, providing direct evidence that the VP1 protein contains neutralizing epitopes independent of other viral capsid proteins; this paves the way for the use of VP1 as a backbone antigen for developing subunit vaccines against EV71. Protection against lethal enterovirus 71 infection in newborn mice by passive immunization with subunit VP1 vaccines and inactivated virus	./cache/cord-282360-byqhzyzi.txt	./txt/cord-282360-byqhzyzi.txt