id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-279716-kxfc4npg	Blachere, Francoise M.	Bioaerosol sampling for the detection of aerosolized influenza virus	2007-10-22		.txt	text/plain	4256	225	50	Background Influenza virus was used to characterize the efficacy of a cyclone‐based, two‐stage personal bioaerosol sampler for the collection and size fractionation of aerosolized viral particles. Results Based on qPCR results, we demonstrate that aerosolized viral particles were efficiently collected and separated according to aerodynamic size using the two‐stage bioaerosol sampler. In order to quantify the relative amount of viral particles or fungal spores collected at each stage of the bioaerosol sampler, qPCR was performed in parallel using either serial 10-fold dilutions of cDNA generated from a single dose of non-aerosolized FluMist Ò containing approximately 10 7 TCID 50 per influenza strain or genomic DNA isolated from 10 7 spores. In this study, by aerosolizing FluMist Ò , we demonstrate the recovery of aerosolized viral particles using the bioaerosol sampler and detection of influenza by qPCR.	./cache/cord-279716-kxfc4npg.txt	./txt/cord-279716-kxfc4npg.txt