id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-002136-mkl89qkt	Nunes, Sandro F.	An ex vivo swine tracheal organ culture for the study of influenza infection	2009-12-09		.txt	text/plain	4719	241	45	Objectives We aimed to develop an air interface EVOC using pig tracheas in the study of influenza infection demonstrating that tracheal explants can be effectively maintained in organ culture and support productive influenza infection. 1, 3 Influenza infection in humans and pigs is primarily restricted to the upper and lower respiratory tract with viral replication occurring in the epithelial cells present on the surface of the respiratory mucosa. Ex vivo organ cultures (EVOC) of tracheal explants with an air interface system have been successfully developed and used in the study of both human and animal respiratory pathogens. To determine if the swine tracheal explants supported productive viral replication, explants were infected with 2AE5 · 10 2 pfu of swine influenza virus and maintained in organ culture for 5 days. Cultures of equine respiratory epithelial cells and organ explants as tools for the study of equine influenza virus infection	./cache/cord-002136-mkl89qkt.txt	./txt/cord-002136-mkl89qkt.txt