id author title date pages extension mime words sentences flesch summary cache txt cord-268480-fd5xi4q1 Rojas, Miguel A. Identification of two novel Rotavirus A genotypes, G35 and P[50], from Peruvian alpaca faeces 2017-09-01 .txt text/plain 1591 103 64 Rotavirus A (RVA) Alp11B was detected from a neonatal Peruvian alpaca presenting with diarrhea, and the Alp11B VP7, VP4, VP6, NSP4, and NSP5 genes were sequenced. Once RVA was detected, the sample was subjected to additional RT-PCR amplifications to identify the VP4, VP7, VP6, NSP4, and NSP5 genotypes using specific primers (Appendix) that were previously published or designed based on RVA sequences available in GenBank. Sequences of Alp11B strain were aligned and compared to that of each corresponding gene of RVA strains obtained from GenBank, by using MegAlign, which are available in the Lasergene software package (DNASTAR, Madison, WI). The nucleotide sequences of the VP4 and VP7 genes from Alp11B were not related to any RVA strain available in GenBank (Fig. 2) . The vicuña strain possesses the unique NSP4-E16 genotype, but the NSP5 gene was not characterized. Infection, Genetics and Evolution 55 (2017) 71-74 E16-H6 with high identity to camelid, bat, and human-like RVA strains. ./cache/cord-268480-fd5xi4q1.txt ./txt/cord-268480-fd5xi4q1.txt