id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-336671-vfq5ft08	Ai, Jing-Wen	Era of molecular diagnosis for pathogen identification of unexplained pneumonia, lessons to be learned	2020-03-16		.txt	text/plain	1883	108	50	Unexplained pneumonia (UP) caused by a novel coronavirus SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) emerged in China in late December 2019 and has infected more than 9000 cases by 31 January 2020. A combinative approach of real-time RT–PCR, CRISPR-based assay and metagenomic next-generation sequencing (mNGS) were used to diagnose this unexplained pneumonia patient. The reasons behind this outbreak are numerous, the highly infectious nature of SARS-CoV-2, limited pathogen detection method for unexplained pneumonia, the high population density of the Hubei Province and across China, etc. On 20 January, Shanghai reported the first imported case of SARS-CoV-2 infection, and through this case, we seek a combinative approach of selected techniques to improve pathogen identification of unexplained pneumonia in the future. We then performed real-time RT-PCR, CRISPR-based assay and metagenomic next-generation sequencing (mNGS) on her respiratory sample and finally diagnosed her with COVID-19.	./cache/cord-336671-vfq5ft08.txt	./txt/cord-336671-vfq5ft08.txt