id author title date pages extension mime words sentences flesch summary cache txt cord-327024-1k5jucae Zhang, Qingshui Isolation and characterization of an astrovirus causing fatal visceral gout in domestic goslings 2018-04-19 .txt text/plain 4167 213 47 18 reported the detection of avian nephritis virus infection in Croatian goose flocks and provided evidence that this AstV was associated with stunting and prehatching mortality of goose embryos. To determine the potential genetic mutation(s) that might occur during the goose embryo passage, the initial virus genome was sequenced using the total RNA extracted from the clinical case tissue homogenate. When the samples were tested by RT-PCR for virus shedding evaluation, the AAstV specific RNA was sequentially detected from the cloacal swabs of infected goslings from 2 to 12 dpi (Fig. 6 ). To evaluate the potential adaptive mutation (s) of the virus that might occur during the process of goose embryo passage, we sequenced the complete genome of initial virus using the total RNA extracted from the clinical case tissue homogenate of kidney, spleen, and liver using the method described above. ./cache/cord-327024-1k5jucae.txt ./txt/cord-327024-1k5jucae.txt