id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-314350-kcatqzgs	Zhang, Xinyu	An emerging novel goose astrovirus associated with gosling gout disease, China	2018-09-05		.txt	text/plain	3534	181	47	Through in vitro and in vivo experiments, we identified and isolated a novel goose astrovirus different from the CAstVas a causative agent of the gout disease recently circulating in gosling flocks in China. To isolate the novel goose astrovirus, the homogenates of the pooled liver and kidney from the diseased gosling were inoculated into chicken liver cell line LMH. To further confirm the isolation of the virus, the infected LMH cells were analyzed through indirect fluorescent assay (IFA) using the convalescent sera from the survival geese with gout symptom and the mouse sera against the capsid P2 of GD (generated in our laboratory) respectively. Different from those recently reported by other groups, we efficiently isolate such novel goose astrovirus in vitro using the cell culture system (LMH cells) and reproduce the associated gout disease by inoculating the cell cultured virus GD.	./cache/cord-314350-kcatqzgs.txt	./txt/cord-314350-kcatqzgs.txt