id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-314189-0rg6n7fr	He, Biao	Intranasal application of polyethyleneimine suppresses influenza virus infection in mice	2016-04-27		.txt	text/plain	1201	64	51	2 Therefore, we hypothesized that intranasal application of a mucosal stimulant that induces antiviral cytokines, except IL-17, may be effective against influenza virus infection. We then examined the viral titers in mouse lungs as previously described 5 and found that PEI significantly reduced lung viral titers on day 2 after H1N1 challenge, whereas the viral titers in the lungs of mice in the CTB-and PBS-pretreated groups showed no significant differences ( Figure 1C ). These results suggest that intranasal application of PEI has efficacy in protecting mice from challenge by influenza virus H1N1. To elucidate the mechanism of action of PEI, we examined the RNA levels of IFN-α4, IFN-β, IFN-γ, GM-CSF, IFITM3 and IL-17 in the lungs of mice pretreated with PEI, CTB, and PBS, respectively, before viral challenge using quantitative reverse transcription-PCR (qRT-PCR). In summary, we demonstrated that PEI, a mucosal stimulant for topical intranasal administration, is highly effective in preventing influenza virus infection.	./cache/cord-314189-0rg6n7fr.txt	./txt/cord-314189-0rg6n7fr.txt