id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-312307-0hqqheho	Ng, Kim Tien	Outbreaks of enterovirus D68 in Malaysia: genetic relatedness to the recent US outbreak strains	2015-08-05		.txt	text/plain	1132	75	53	Specimens positive for enteroviruses were further confirmed using standard molecular approaches that involved amplification and sequencing of the human enterovirus VP4/VP2 gene using primers described previously. Based on previously described EV-D68 classification, 11 the newly sequenced strains from Malaysia were found within clade A (MY-Cluster-1) and clade B (MY-Cluster-2). Phylogenetic analysis of the P1 region indicated that 91.7% (11/12) of the Malaysian EV-D68 formed clusters, suggesting the transient EV-D68 outbreaks were most likely caused by at least two viral lineages ( Figure 1) . Such observation suggests an ongoing ''clade shift'' or lineage replacement of circulating EV-D68 in causing new outbreak, as observed in other enterovirus-associated outbreaks. Our data suggest that the recent EV-D68 strains associated with unprecedented severe respiratory outbreaks in the USA in 2014 were probably descended from the recent EV-D68 lineages circulating in Thailand and Malaysia. Seven strains of enterovirus D68 detected in the United States during the 2014 severe respiratory disease outbreak	./cache/cord-312307-0hqqheho.txt	./txt/cord-312307-0hqqheho.txt