id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-003573-ekxq4l9a	Yao, Yanfeng	Protection against homo and hetero-subtypic influenza A virus by optimized M2e DNA vaccine	2019-01-16		.txt	text/plain	5336	287	53	In this study, we used N-terminal extracellular region of the influenza virus M2 protein (M2e) as the target antigen and constructed two optimized M2e DNA vaccines (p-tPA-p3M2e and p-p3M2e) with increased antigenic epitope density and enhanced antigen secretion. Our results show that the serum of mice immunized with p-tPA-p3M2e and p-p3M2e specifically binds to H1N1-infected MDCK cells, indicating that the M2e-specific antibodies induced by p-tPA-p3M2e and p-p3M2e immunization recognize the M2 protein on the surface of cells infected with influenza virus ( Figure S1 ). The above results demonstrate that vaccination with p-tPA-p3M2e protects mice from lethal infection with heterologous avian influenza strains, especially the H9N2 virus. In the present study, high antibody titers were induced in the p-tPA-p3M2e and p-p3M2e immunization groups, both of which demonstrated increased protection against challenge with homologous virus (H1N1). Immunization with p-tPA-p3M2e, without any adjuvant, induced high level, M2e-specific antibody production, humoral/cellular immune responses, and protected BALB/c mice from lethal infections of homo-and hetero-subtypic viruses.	./cache/cord-003573-ekxq4l9a.txt	./txt/cord-003573-ekxq4l9a.txt