key: cord-256853-2s31fn04
authors: Jin, Cheng Cheng; Zhu, Li; Gao, Chun; Zhang, Sheng
title: Correlation between viral RNA shedding and serum antibodies in COVID-19 patients
date: 2020-05-23
journal: Clin Microbiol Infect
DOI: 10.1016/j.cmi.2020.05.022
sha: 
doc_id: 256853
cord_uid: 2s31fn04

nan

shedding.

[2] Based on currently available information, the relationship between the 6 dynamic of serum antibodies and viral replication is unclear. In this study, we 7 investigated the correlation between serum antibodies and duration of viral RNA 8 shedding. 9

We retrospectively enrolled 89 hospitalized patients (admission date from Jan 22 nd 10 to Feb 13 rd , 2020) with laboratory confirmed SARS-CoV-2 infection in Tongji Hospital 11 of Huazhong University of Science and Technology in Wuhan, China. We enrolled 12 patients who had blood tests intended for antibody detection during hospitalization. All 13 patients had mild to moderate illness, and did not required intubation or ICU admission. 14 Throat and/or nasal swabs collected upon admission and during hospitalizion were 15 analyzed by SARS-CoV-2 real-time reverse transcription polymerase chain reaction 16 (RT-PCR) according to the manufacturer's protocol (Shanghai Huirui Biotechnology 17

Co., Ltd). Specific antibodies IgM and IgG to SAR-CoV-2 were analyzed by 18 chemiluminescent immunoassay (CLIA) according to the manufacturer's protocol 19 (Shenzhen Yahuilong Biotechnology Co., Ltd). The kits had two antigens of SARS-20

CoV-2 coated on the magnetic beads (Nucleocapsid protein or N protein, Spike Protein 21 or S proteins). iFlash3000 fully automatic chemiluminescence immunoassay analyzer 22 (Shenzhen Yahuilong, Biotechnology Co., Ltd) was applied to analyze the samples. 23

Serum IgM and IgG titer (AU/ml) was calculated by the immunoassay analyzer. 13.2±4.0 AU/ml) declined almost to the reference level (10 AU/ml). In prolonged 39 group, serum IgG was slightly higher than that in the other group through week 4 to 40

week 8. However, the difference between two groups was not significant (p>0.05). 41 

Profile of Specific Antibodies to SARS-CoV-2: 98