id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-346467-a0r4xh1c	Cornelissen, Jan B. W. J.	Mycoplasma detection by triplex real-time PCR in bronchoalveolar lavage fluid from bovine respiratory disease complex cases	2017-04-08		.txt	text/plain	4082	188	50	title: Mycoplasma detection by triplex real-time PCR in bronchoalveolar lavage fluid from bovine respiratory disease complex cases BACKGROUND: In this study we evaluated the RespoCheck Mycoplasma triplex real-time PCR for the detection in bronchoalveolar lavage fluid (BALF) of Mycoplasma (M.) dispar, M. RESULTS: The analytical sensitivity of the RespoCheck triplex real-time PCR was, as determined by spiking experiments of the Mycoplasma strains in Phosphate Buffered Saline, 300 colony forming units (cfu)/mL for M. To enable testing of testing for BRD associated pathogens in a routine setting, real-time PCRs for detection of viral, bacterial and mycoplasma pathogens in bronchoalveolar lavage fluid (BALF) of calves have been set up by the Central Veterinary Institute (Lelystad, The Netherlands) under the name RespoCheck. In this study we used the highly conserved 16S rRNA sequence to set up the RespoCheck Mycoplasma triplex real-time PCR assay for the specific detection of M.	./cache/cord-346467-a0r4xh1c.txt	./txt/cord-346467-a0r4xh1c.txt