id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-346250-9kiekksx	Khare, Sangeeta	Vaccination with attenuated Salmonella enterica Dublin expressing E coli O157:H7 outer membrane protein Intimin induces transient reduction of fecal shedding of E coli O157:H7 in cattle	2010-07-07		.txt	text/plain	5565	335	51	title: Vaccination with attenuated Salmonella enterica Dublin expressing E coli O157:H7 outer membrane protein Intimin induces transient reduction of fecal shedding of E coli O157:H7 in cattle The present study was undertaken to test the hypothesis that an adaptive mucosal immune response directed against intimin will reduce or prevent enteric colonization and fecal shedding of E. During the first 28 days, vaccinated calves shed both the vector strain and the intimin-expressing S. Calves challenged with intimin-deficient mutant bacteria do not develop diarrhea or attaching/ effacing lesions, nor are colonized to the same extent as animals infected with wild type or complemented mutant strains [20] . Fecal samples from all the calves were collected daily from day 0 to day 42 post-inoculation to determine the level of vaccine strain shedding. Immunization of cattle with a combination of purified intimin-531, EspA and Tir significantly reduces shedding of Escherichia coli O157:H7 following oral challenge	./cache/cord-346250-9kiekksx.txt	./txt/cord-346250-9kiekksx.txt