id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-329148-zs18ez5q	Geng, Yunyun	Development of real-time recombinase polymerase amplification assay for rapid and sensitive detection of canine parvovirus 2	2017-11-06		.txt	text/plain	3889	193	55	title: Development of real-time recombinase polymerase amplification assay for rapid and sensitive detection of canine parvovirus 2 With the advances in molecular detection techniques, a substantial number of gene amplification-based assays have been described for CPV-2 diagnosis such as polymerase chain reaction (PCR), nested PCR, real-time PCR, reverse-transcription loop-mediated isothermal amplification (RT-LAMP) and insulated isothermal PCR (iiPCR) [9] [10] [11] [12] [13] [14] [15] . Based on our previous study, we developed here a real-time RPA assay for simple, rapid, convenient and POC detection of CPV-2, which utilizes an exo probe and a portable, userfriendly POC tube scanner. A probit regression analysis using the results of eight complete molecular standard runs calculated that the limit of detection (LOD) of the real-time RPA was 10 1 copies per reaction in 95% of cases (Fig. 2c) , which was the same as that of the real-time PCR applied in the study (data not shown).	./cache/cord-329148-zs18ez5q.txt	./txt/cord-329148-zs18ez5q.txt