id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-003587-zminzrov	Wang, Xueyu	Visual detection of porcine epidemic diarrhea virus using a novel reverse transcription polymerase spiral reaction method	2019-04-15		.txt	text/plain	3633	173	48	title: Visual detection of porcine epidemic diarrhea virus using a novel reverse transcription polymerase spiral reaction method The aim of this study was to establish a novel reverse transcription polymerase spiral reaction (RT-PSR) assay for the rapid detection of porcine epidemic diarrhea virus (PEDV). This new method for PEDV detection was 10 times more sensitive than the conventional reverse transcription-polymerase chain reaction (RT-PCR) assay. To decrease the time required for PEDV detection, PCR-related methods focused on the amplification of viral nucleic acids have been developed, which have been shown to be more efficient, highly sensitive and specific, even at different stages of the disease, when compared to immunological diagnostic methods. The polymerase spiral reaction (PSR) [14] is a novel nucleic acid isothermal amplification method that has the advantages of simplicity, rapidity, accuracy, and low cost when compared to conventional PCR.	./cache/cord-003587-zminzrov.txt	./txt/cord-003587-zminzrov.txt