id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-336975-28mtmw2z	Sadeghi, Christine D	Twelve years' detection of respiratory viruses by immunofluorescence in hospitalised children: impact of the introduction of a new respiratory picornavirus assay	2011-02-07		.txt	text/plain	3032	161	41	PCR-based studies have suggested the important role of respiratory picornaviruses (rhinovirus and enterovirus) as a leading cause of lower respiratory tract infections in children [5] , in particular wheezing illnesses such as bronchiolitis [6, 7] , wheezy bronchitis [8] and asthma exacerbations [9] , but also pneumonia [2] . In addition, PCR has allowed for the detection of new respiratory viruses, such as hMPV [10] , which has been implicated in upper and lower respiratory tract infections in children [11] [12] [13] . A significant proportion of asymptomatic children test positive by PCR to respiratory viruses [14] [15] [16] , and picornavirus RNA can be detected by PCR up to 5 weeks after an acute infection [17] . In order to determine the value of DFA in conducting epidemiological studies on respiratory viruses now that assays for respiratory picornaviruses and hMPV are available, we retrospectively analysed the results of 12 years of DFA screening of viral pathogens in hospitalized children with respiratory disease.	./cache/cord-336975-28mtmw2z.txt	./txt/cord-336975-28mtmw2z.txt