id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-324638-gwd8qin6	Chiu, Rossa WK	Automated extraction protocol for quantification of SARS-Coronavirus RNA in serum: an evaluation study	2006-02-09		.txt	text/plain	3360	152	49	We developed a modified protocol in compliance with the recommended biosafety guidelines from the World Health Organization based on the use of the MagNA Pure total nucleic acid large volume isolation kit for the extraction of SARS-coronavirus RNA. The main objective of this study was to compare the resultant analytical sensitivity and quantitative performance of the serum SARS-CoV RNA test when either the manual or automated extraction protocol was used. The modified large volume protocol with the external lysis step was further compared with the external lysis protocol of the total nucleic acid isolation kit using a transport medium mixture containing 10 6 copies/mL of inactivated SARS-CoV. Serially diluted inactivated SARS-CoV isolate in transport medium was extracted by both the column-based manual method and the MagNA Pure LC instrument using the modified large volume protocol with external lysis.	./cache/cord-324638-gwd8qin6.txt	./txt/cord-324638-gwd8qin6.txt