id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-276857-i948aq4b	Chung, Grace TY	A simple and rapid approach for screening of SARS-coronavirus genotypes: an evaluation study	2005-10-18		.txt	text/plain	2331	129	50	We present here a simple and rapid assay for the screening of SARS-coronavirus genotypes based on the use of fluorogenic oligonucleotide probes for allelic discrimination. Genotyping of the SARS-coronavirus isolates obtained from these patients were carried out by the allelic discrimination assays and confirmed by direct sequencing. In-depth analysis of the available sequence data on SARS-CoV also revealed that the viral isolates could be readily subclassified into several major genotypes based on nucleotide variations at specific genomic positions [8, 12] . In this study, we demonstrate the feasibility of the adoption of allelic discrimination assays based on the use of fluorogenic oligonucleotide probes for the genotyping of SARS-CoV isolates. Our study has clearly demonstrated the feasibility of using allelic discrimination assays as a method for genetic characterization of SARS-CoV genotypes in patients. Genotype of SARS-CoV culture isolates from 30 patients determined by Taqman Allelic Discrimination assays Petric M, Skowronski DM	./cache/cord-276857-i948aq4b.txt	./txt/cord-276857-i948aq4b.txt