id	sid	tid	token	lemma	pos
cord-283109-ka3n9pft	1	1	key	key	JJ
cord-283109-ka3n9pft	1	2	:	:	:
cord-283109-ka3n9pft	1	3	cord-283109-ka3n9pft	cord-283109-ka3n9pft	NNS
cord-283109-ka3n9pft	1	4	authors	author	NNS
cord-283109-ka3n9pft	1	5	:	:	:
cord-283109-ka3n9pft	1	6	Arumugam	Arumugam	NNP
cord-283109-ka3n9pft	1	7	,	,	,
cord-283109-ka3n9pft	1	8	Arunkumar	Arunkumar	NNP
cord-283109-ka3n9pft	1	9	;	;	:
cord-283109-ka3n9pft	1	10	Wong	Wong	NNP
cord-283109-ka3n9pft	1	11	,	,	,
cord-283109-ka3n9pft	1	12	Season	Season	NNP
cord-283109-ka3n9pft	1	13	title	title	NN
cord-283109-ka3n9pft	1	14	:	:	:
cord-283109-ka3n9pft	1	15	The	the	DT
cord-283109-ka3n9pft	1	16	Potential	potential	JJ
cord-283109-ka3n9pft	1	17	Use	Use	NNP
cord-283109-ka3n9pft	1	18	of	of	IN
cord-283109-ka3n9pft	1	19	Unprocessed	unprocessed	JJ
cord-283109-ka3n9pft	1	20	Sample	Sample	NNP
cord-283109-ka3n9pft	1	21	for	for	IN
cord-283109-ka3n9pft	1	22	RT	RT	NNP
cord-283109-ka3n9pft	1	23	-	-	HYPH
cord-283109-ka3n9pft	1	24	qPCR	qPCR	NNP
cord-283109-ka3n9pft	1	25	Detection	Detection	NNP
cord-283109-ka3n9pft	1	26	of	of	IN
cord-283109-ka3n9pft	1	27	COVID-19	COVID-19	NNP
cord-283109-ka3n9pft	1	28	without	without	IN
cord-283109-ka3n9pft	1	29	an	an	DT
cord-283109-ka3n9pft	1	30	RNA	RNA	NNP
cord-283109-ka3n9pft	1	31	Extraction	Extraction	NNP
cord-283109-ka3n9pft	1	32	Step	Step	NNP
cord-283109-ka3n9pft	1	33	date	date	NN
cord-283109-ka3n9pft	1	34	:	:	:
cord-283109-ka3n9pft	1	35	2020	2020	CD
cord-283109-ka3n9pft	1	36	-	-	HYPH
cord-283109-ka3n9pft	1	37	04	04	CD
cord-283109-ka3n9pft	1	38	-	-	HYPH
cord-283109-ka3n9pft	1	39	08	08	CD
cord-283109-ka3n9pft	1	40	journal	journal	NN
cord-283109-ka3n9pft	1	41	:	:	:
cord-283109-ka3n9pft	2	1	bioRxiv	biorxiv	IN
cord-283109-ka3n9pft	2	2	DOI	DOI	NNP
cord-283109-ka3n9pft	2	3	:	:	:
cord-283109-ka3n9pft	3	1	10.1101/2020.04.06.028811	10.1101/2020.04.06.028811	CD
cord-283109-ka3n9pft	3	2	sha	sha	NN
cord-283109-ka3n9pft	3	3	:	:	:
cord-283109-ka3n9pft	3	4	637a3428061da47914f6dabed131e9a523f69851	637a3428061da47914f6dabed131e9a523f69851	CD
cord-283109-ka3n9pft	3	5	doc_id	doc_id	CD
cord-283109-ka3n9pft	3	6	:	:	:
cord-283109-ka3n9pft	3	7	283109	283109	CD
cord-283109-ka3n9pft	3	8	cord_uid	cord_uid	NNS
cord-283109-ka3n9pft	3	9	:	:	:
cord-283109-ka3n9pft	4	1	ka3n9pft	ka3n9pft	NNP
cord-283109-ka3n9pft	4	2	Quantitative	quantitative	JJ
cord-283109-ka3n9pft	4	3	reverse	reverse	NN
cord-283109-ka3n9pft	4	4	transcription	transcription	NN
cord-283109-ka3n9pft	4	5	polymerase	polymerase	NN
cord-283109-ka3n9pft	4	6	chain	chain	NN
cord-283109-ka3n9pft	4	7	reaction	reaction	NN
cord-283109-ka3n9pft	5	1	(	(	-LRB-
cord-283109-ka3n9pft	5	2	RT	RT	NNP
cord-283109-ka3n9pft	5	3	-	-	HYPH
cord-283109-ka3n9pft	5	4	qPCR	qPCR	NNP
cord-283109-ka3n9pft	5	5	)	)	-RRB-
cord-283109-ka3n9pft	5	6	assay	assay	NN
cord-283109-ka3n9pft	5	7	is	be	VBZ
cord-283109-ka3n9pft	5	8	the	the	DT
cord-283109-ka3n9pft	5	9	gold	gold	JJ
cord-283109-ka3n9pft	5	10	standard	standard	NN
cord-283109-ka3n9pft	5	11	recommended	recommend	VBN
cord-283109-ka3n9pft	5	12	to	to	TO
cord-283109-ka3n9pft	5	13	test	test	VB
cord-283109-ka3n9pft	5	14	for	for	IN
cord-283109-ka3n9pft	5	15	acute	acute	JJ
cord-283109-ka3n9pft	5	16	SARS	SARS	NNP
cord-283109-ka3n9pft	5	17	-	-	HYPH
cord-283109-ka3n9pft	5	18	CoV-2	CoV-2	NNP
cord-283109-ka3n9pft	5	19	infection.1–4	infection.1–4	NNP
cord-283109-ka3n9pft	6	1	It	-PRON-	PRP
cord-283109-ka3n9pft	6	2	has	have	VBZ
cord-283109-ka3n9pft	6	3	been	be	VBN
cord-283109-ka3n9pft	6	4	used	use	VBN
cord-283109-ka3n9pft	6	5	by	by	IN
cord-283109-ka3n9pft	6	6	the	the	DT
cord-283109-ka3n9pft	6	7	Centers	Centers	NNPS
cord-283109-ka3n9pft	6	8	for	for	IN
cord-283109-ka3n9pft	6	9	Disease	Disease	NNP
cord-283109-ka3n9pft	6	10	Control	Control	NNP
cord-283109-ka3n9pft	6	11	and	and	CC
cord-283109-ka3n9pft	6	12	Prevention	Prevention	NNP
cord-283109-ka3n9pft	6	13	(	(	-LRB-
cord-283109-ka3n9pft	6	14	CDC	CDC	NNP
cord-283109-ka3n9pft	6	15	)	)	-RRB-
cord-283109-ka3n9pft	6	16	and	and	CC
cord-283109-ka3n9pft	6	17	several	several	JJ
cord-283109-ka3n9pft	6	18	other	other	JJ
cord-283109-ka3n9pft	6	19	companies	company	NNS
cord-283109-ka3n9pft	6	20	in	in	IN
cord-283109-ka3n9pft	6	21	their	-PRON-	PRP$
cord-283109-ka3n9pft	6	22	Emergency	Emergency	NNP
cord-283109-ka3n9pft	6	23	Use	Use	NNP
cord-283109-ka3n9pft	6	24	Authorization	Authorization	NNP
cord-283109-ka3n9pft	6	25	(	(	-LRB-
cord-283109-ka3n9pft	6	26	EUA	EUA	NNP
cord-283109-ka3n9pft	6	27	)	)	-RRB-
cord-283109-ka3n9pft	6	28	assays	assay	NNS
cord-283109-ka3n9pft	6	29	.	.	.
cord-283109-ka3n9pft	7	1	With	with	IN
cord-283109-ka3n9pft	7	2	many	many	JJ
cord-283109-ka3n9pft	7	3	PCR	pcr	NN
cord-283109-ka3n9pft	7	4	-	-	HYPH
cord-283109-ka3n9pft	7	5	based	base	VBN
cord-283109-ka3n9pft	7	6	molecular	molecular	JJ
cord-283109-ka3n9pft	7	7	assays	assay	NNS
cord-283109-ka3n9pft	7	8	,	,	,
cord-283109-ka3n9pft	7	9	an	an	DT
cord-283109-ka3n9pft	7	10	extraction	extraction	NN
cord-283109-ka3n9pft	7	11	step	step	NN
cord-283109-ka3n9pft	7	12	is	be	VBZ
cord-283109-ka3n9pft	7	13	routinely	routinely	RB
cord-283109-ka3n9pft	7	14	used	use	VBN
cord-283109-ka3n9pft	7	15	as	as	IN
cord-283109-ka3n9pft	7	16	part	part	NN
cord-283109-ka3n9pft	7	17	of	of	IN
cord-283109-ka3n9pft	7	18	the	the	DT
cord-283109-ka3n9pft	7	19	protocol	protocol	NN
cord-283109-ka3n9pft	7	20	.	.	.
cord-283109-ka3n9pft	8	1	This	this	DT
cord-283109-ka3n9pft	8	2	step	step	NN
cord-283109-ka3n9pft	8	3	can	can	MD
cord-283109-ka3n9pft	8	4	take	take	VB
cord-283109-ka3n9pft	8	5	up	up	RP
cord-283109-ka3n9pft	8	6	a	a	DT
cord-283109-ka3n9pft	8	7	significant	significant	JJ
cord-283109-ka3n9pft	8	8	amount	amount	NN
cord-283109-ka3n9pft	8	9	of	of	IN
cord-283109-ka3n9pft	8	10	time	time	NN
cord-283109-ka3n9pft	8	11	and	and	CC
cord-283109-ka3n9pft	8	12	labor	labor	NN
cord-283109-ka3n9pft	8	13	,	,	,
cord-283109-ka3n9pft	8	14	especially	especially	RB
cord-283109-ka3n9pft	8	15	if	if	IN
cord-283109-ka3n9pft	8	16	the	the	DT
cord-283109-ka3n9pft	8	17	extraction	extraction	NN
cord-283109-ka3n9pft	8	18	is	be	VBZ
cord-283109-ka3n9pft	8	19	performed	perform	VBN
cord-283109-ka3n9pft	8	20	manually	manually	RB
cord-283109-ka3n9pft	8	21	.	.	.
cord-283109-ka3n9pft	9	1	Long	long	JJ
cord-283109-ka3n9pft	9	2	assay	assay	NN
cord-283109-ka3n9pft	9	3	time	time	NN
cord-283109-ka3n9pft	9	4	,	,	,
cord-283109-ka3n9pft	9	5	partly	partly	RB
cord-283109-ka3n9pft	9	6	caused	cause	VBN
cord-283109-ka3n9pft	9	7	by	by	IN
cord-283109-ka3n9pft	9	8	slow	slow	JJ
cord-283109-ka3n9pft	9	9	sample	sample	NN
cord-283109-ka3n9pft	9	10	preparation	preparation	NN
cord-283109-ka3n9pft	9	11	steps	step	NNS
cord-283109-ka3n9pft	9	12	,	,	,
cord-283109-ka3n9pft	9	13	has	have	VBZ
cord-283109-ka3n9pft	9	14	created	create	VBN
cord-283109-ka3n9pft	9	15	a	a	DT
cord-283109-ka3n9pft	9	16	large	large	JJ
cord-283109-ka3n9pft	9	17	backlog	backlog	NN
cord-283109-ka3n9pft	9	18	when	when	WRB
cord-283109-ka3n9pft	9	19	testing	test	VBG
cord-283109-ka3n9pft	9	20	patient	patient	NN
cord-283109-ka3n9pft	9	21	samples	sample	NNS
cord-283109-ka3n9pft	9	22	suspected	suspect	VBN
cord-283109-ka3n9pft	9	23	of	of	IN
cord-283109-ka3n9pft	9	24	COVID-19	COVID-19	NNP
cord-283109-ka3n9pft	9	25	.	.	.
cord-283109-ka3n9pft	10	1	Using	use	VBG
cord-283109-ka3n9pft	10	2	flu	flu	NN
cord-283109-ka3n9pft	10	3	and	and	CC
cord-283109-ka3n9pft	10	4	RSV	RSV	NNP
cord-283109-ka3n9pft	10	5	clinical	clinical	JJ
cord-283109-ka3n9pft	10	6	specimens	specimen	NNS
cord-283109-ka3n9pft	10	7	,	,	,
cord-283109-ka3n9pft	10	8	we	-PRON-	PRP
cord-283109-ka3n9pft	10	9	have	have	VBP
cord-283109-ka3n9pft	10	10	collected	collect	VBN
cord-283109-ka3n9pft	10	11	evidence	evidence	NN
cord-283109-ka3n9pft	10	12	that	that	IN
cord-283109-ka3n9pft	10	13	the	the	DT
cord-283109-ka3n9pft	10	14	RT	RT	NNP
cord-283109-ka3n9pft	10	15	-	-	HYPH
cord-283109-ka3n9pft	10	16	qPCR	qpcr	NN
cord-283109-ka3n9pft	10	17	assay	assay	NN
cord-283109-ka3n9pft	10	18	can	can	MD
cord-283109-ka3n9pft	10	19	be	be	VB
cord-283109-ka3n9pft	10	20	performed	perform	VBN
cord-283109-ka3n9pft	10	21	directly	directly	RB
cord-283109-ka3n9pft	10	22	on	on	IN
cord-283109-ka3n9pft	10	23	patient	patient	JJ
cord-283109-ka3n9pft	10	24	sample	sample	NN
cord-283109-ka3n9pft	10	25	material	material	NN
cord-283109-ka3n9pft	10	26	from	from	IN
cord-283109-ka3n9pft	10	27	a	a	DT
cord-283109-ka3n9pft	10	28	nasal	nasal	NN
cord-283109-ka3n9pft	10	29	swab	swab	NN
cord-283109-ka3n9pft	10	30	immersed	immerse	VBN
cord-283109-ka3n9pft	10	31	in	in	IN
cord-283109-ka3n9pft	10	32	virus	virus	NN
cord-283109-ka3n9pft	10	33	transport	transport	NN
cord-283109-ka3n9pft	10	34	medium	medium	NN
cord-283109-ka3n9pft	10	35	(	(	-LRB-
cord-283109-ka3n9pft	10	36	VTM	VTM	NNP
cord-283109-ka3n9pft	10	37	)	)	-RRB-
cord-283109-ka3n9pft	10	38	without	without	IN
cord-283109-ka3n9pft	10	39	an	an	DT
cord-283109-ka3n9pft	10	40	RNA	RNA	NNP
cord-283109-ka3n9pft	10	41	extraction	extraction	NN
cord-283109-ka3n9pft	10	42	step	step	NN
cord-283109-ka3n9pft	10	43	.	.	.
cord-283109-ka3n9pft	11	1	We	-PRON-	PRP
cord-283109-ka3n9pft	11	2	have	have	VBP
cord-283109-ka3n9pft	11	3	also	also	RB
cord-283109-ka3n9pft	11	4	used	use	VBN
cord-283109-ka3n9pft	11	5	this	this	DT
cord-283109-ka3n9pft	11	6	approach	approach	NN
cord-283109-ka3n9pft	11	7	to	to	TO
cord-283109-ka3n9pft	11	8	test	test	VB
cord-283109-ka3n9pft	11	9	for	for	IN
cord-283109-ka3n9pft	11	10	the	the	DT
cord-283109-ka3n9pft	11	11	direct	direct	JJ
cord-283109-ka3n9pft	11	12	detection	detection	NN
cord-283109-ka3n9pft	11	13	of	of	IN
cord-283109-ka3n9pft	11	14	SARS	SARS	NNP
cord-283109-ka3n9pft	11	15	-	-	HYPH
cord-283109-ka3n9pft	11	16	CoV-2	CoV-2	NNP
cord-283109-ka3n9pft	11	17	reference	reference	NN
cord-283109-ka3n9pft	11	18	materials	material	NNS
cord-283109-ka3n9pft	11	19	spiked	spike	VBN
cord-283109-ka3n9pft	11	20	in	in	IN
cord-283109-ka3n9pft	11	21	VTM	VTM	NNP
cord-283109-ka3n9pft	11	22	.	.	.
cord-283109-ka3n9pft	12	1	Our	-PRON-	PRP$
cord-283109-ka3n9pft	12	2	data	datum	NNS
cord-283109-ka3n9pft	12	3	,	,	,
cord-283109-ka3n9pft	12	4	while	while	IN
cord-283109-ka3n9pft	12	5	preliminary	preliminary	JJ
cord-283109-ka3n9pft	12	6	,	,	,
cord-283109-ka3n9pft	12	7	suggest	suggest	VBP
cord-283109-ka3n9pft	12	8	that	that	IN
cord-283109-ka3n9pft	12	9	using	use	VBG
cord-283109-ka3n9pft	12	10	a	a	DT
cord-283109-ka3n9pft	12	11	few	few	JJ
cord-283109-ka3n9pft	12	12	microliters	microliter	NNS
cord-283109-ka3n9pft	12	13	of	of	IN
cord-283109-ka3n9pft	12	14	these	these	DT
cord-283109-ka3n9pft	12	15	untreated	untreated	JJ
cord-283109-ka3n9pft	12	16	samples	sample	NNS
cord-283109-ka3n9pft	12	17	still	still	RB
cord-283109-ka3n9pft	12	18	can	can	MD
cord-283109-ka3n9pft	12	19	lead	lead	VB
cord-283109-ka3n9pft	12	20	to	to	IN
cord-283109-ka3n9pft	12	21	sensitive	sensitive	JJ
cord-283109-ka3n9pft	12	22	test	test	NN
cord-283109-ka3n9pft	12	23	results	result	NNS
cord-283109-ka3n9pft	12	24	.	.	.
cord-283109-ka3n9pft	13	1	If	if	IN
cord-283109-ka3n9pft	13	2	RNA	RNA	NNP
cord-283109-ka3n9pft	13	3	extraction	extraction	NN
cord-283109-ka3n9pft	13	4	steps	step	NNS
cord-283109-ka3n9pft	13	5	can	can	MD
cord-283109-ka3n9pft	13	6	be	be	VB
cord-283109-ka3n9pft	13	7	omitted	omit	VBN
cord-283109-ka3n9pft	13	8	without	without	IN
cord-283109-ka3n9pft	13	9	significantly	significantly	RB
cord-283109-ka3n9pft	13	10	affecting	affect	VBG
cord-283109-ka3n9pft	13	11	clinical	clinical	JJ
cord-283109-ka3n9pft	13	12	sensitivity	sensitivity	NN
cord-283109-ka3n9pft	13	13	,	,	,
cord-283109-ka3n9pft	13	14	the	the	DT
cord-283109-ka3n9pft	13	15	turn	turn	NN
cord-283109-ka3n9pft	13	16	-	-	HYPH
cord-283109-ka3n9pft	13	17	around	around	RP
cord-283109-ka3n9pft	13	18	time	time	NN
cord-283109-ka3n9pft	13	19	of	of	IN
cord-283109-ka3n9pft	13	20	COVID-19	covid-19	JJ
cord-283109-ka3n9pft	13	21	tests	test	NNS
cord-283109-ka3n9pft	13	22	and	and	CC
cord-283109-ka3n9pft	13	23	the	the	DT
cord-283109-ka3n9pft	13	24	backlog	backlog	NN
cord-283109-ka3n9pft	13	25	we	-PRON-	PRP
cord-283109-ka3n9pft	13	26	currently	currently	RB
cord-283109-ka3n9pft	13	27	experience	experience	VBP
cord-283109-ka3n9pft	13	28	can	can	MD
cord-283109-ka3n9pft	13	29	be	be	VB
cord-283109-ka3n9pft	13	30	reduced	reduce	VBN
cord-283109-ka3n9pft	13	31	drastically	drastically	RB
cord-283109-ka3n9pft	13	32	.	.	.
cord-283109-ka3n9pft	14	1	Next	next	RB
cord-283109-ka3n9pft	14	2	,	,	,
cord-283109-ka3n9pft	14	3	we	-PRON-	PRP
cord-283109-ka3n9pft	14	4	will	will	MD
cord-283109-ka3n9pft	14	5	confirm	confirm	VB
cord-283109-ka3n9pft	14	6	our	-PRON-	PRP$
cord-283109-ka3n9pft	14	7	findings	finding	NNS
cord-283109-ka3n9pft	14	8	using	use	VBG
cord-283109-ka3n9pft	14	9	patient	patient	JJ
cord-283109-ka3n9pft	14	10	samples	sample	NNS
cord-283109-ka3n9pft	14	11	.	.	.
cord-283109-ka3n9pft	15	1	If	if	IN
cord-283109-ka3n9pft	15	2	RNA	RNA	NNP
cord-283109-ka3n9pft	15	3	extraction	extraction	NN
cord-283109-ka3n9pft	15	4	steps	step	NNS
cord-283109-ka3n9pft	15	5	can	can	MD
cord-283109-ka3n9pft	15	6	be	be	VB
cord-283109-ka3n9pft	15	7	omitted	omit	VBN
cord-283109-ka3n9pft	15	8	without	without	IN
cord-283109-ka3n9pft	15	9	significantly	significantly	RB
cord-283109-ka3n9pft	15	10	affecting	affect	VBG
cord-283109-ka3n9pft	15	11	clinical	clinical	JJ
cord-283109-ka3n9pft	15	12	sensitivity	sensitivity	NN
cord-283109-ka3n9pft	15	13	,	,	,
cord-283109-ka3n9pft	15	14	the	the	DT
cord-283109-ka3n9pft	15	15	turnaround	turnaround	NN
cord-283109-ka3n9pft	15	16	time	time	NN
cord-283109-ka3n9pft	15	17	of	of	IN
cord-283109-ka3n9pft	15	18	COVID-19	covid-19	JJ
cord-283109-ka3n9pft	15	19	tests	test	NNS
cord-283109-ka3n9pft	15	20	and	and	CC
cord-283109-ka3n9pft	15	21	the	the	DT
cord-283109-ka3n9pft	15	22	backlog	backlog	NN
cord-283109-ka3n9pft	15	23	we	-PRON-	PRP
cord-283109-ka3n9pft	15	24	currently	currently	RB
cord-283109-ka3n9pft	15	25	experience	experience	VBP
cord-283109-ka3n9pft	15	26	can	can	MD
cord-283109-ka3n9pft	15	27	be	be	VB
cord-283109-ka3n9pft	15	28	reduced	reduce	VBN
cord-283109-ka3n9pft	15	29	drastically	drastically	RB
cord-283109-ka3n9pft	15	30	.	.	.
cord-283109-ka3n9pft	16	1	Next	next	RB
cord-283109-ka3n9pft	16	2	,	,	,
cord-283109-ka3n9pft	16	3	we	-PRON-	PRP
cord-283109-ka3n9pft	16	4	will	will	MD
cord-283109-ka3n9pft	16	5	confirm	confirm	VB
cord-283109-ka3n9pft	16	6	our	-PRON-	PRP$
cord-283109-ka3n9pft	16	7	findings	finding	NNS
cord-283109-ka3n9pft	16	8	using	use	VBG
cord-283109-ka3n9pft	16	9	patient	patient	JJ
cord-283109-ka3n9pft	16	10	samples	sample	NNS
cord-283109-ka3n9pft	16	11	.	.	.
cord-283109-ka3n9pft	17	1	The	the	DT
cord-283109-ka3n9pft	17	2	sample	sample	NN
cord-283109-ka3n9pft	17	3	preparation	preparation	NN
cord-283109-ka3n9pft	17	4	step	step	NN
cord-283109-ka3n9pft	17	5	is	be	VBZ
cord-283109-ka3n9pft	17	6	generally	generally	RB
cord-283109-ka3n9pft	17	7	time	time	NN
cord-283109-ka3n9pft	17	8	-	-	HYPH
cord-283109-ka3n9pft	17	9	consuming	consume	VBG
cord-283109-ka3n9pft	17	10	,	,	,
cord-283109-ka3n9pft	17	11	regardless	regardless	RB
cord-283109-ka3n9pft	17	12	of	of	IN
cord-283109-ka3n9pft	17	13	whether	whether	IN
cord-283109-ka3n9pft	17	14	it	-PRON-	PRP
cord-283109-ka3n9pft	17	15	is	be	VBZ
cord-283109-ka3n9pft	17	16	done	do	VBN
cord-283109-ka3n9pft	17	17	manually	manually	RB
cord-283109-ka3n9pft	17	18	or	or	CC
cord-283109-ka3n9pft	17	19	automated	automate	VBN
cord-283109-ka3n9pft	17	20	.	.	.
cord-283109-ka3n9pft	18	1	In	in	IN
cord-283109-ka3n9pft	18	2	addition	addition	NN
cord-283109-ka3n9pft	18	3	,	,	,
cord-283109-ka3n9pft	18	4	there	there	EX
cord-283109-ka3n9pft	18	5	is	be	VBZ
cord-283109-ka3n9pft	18	6	a	a	DT
cord-283109-ka3n9pft	18	7	current	current	JJ
cord-283109-ka3n9pft	18	8	shortage	shortage	NN
cord-283109-ka3n9pft	18	9	of	of	IN
cord-283109-ka3n9pft	18	10	the	the	DT
cord-283109-ka3n9pft	18	11	recommended	recommend	VBN
cord-283109-ka3n9pft	18	12	viral	viral	JJ
cord-283109-ka3n9pft	18	13	RNA	RNA	NNP
cord-283109-ka3n9pft	18	14	extraction	extraction	NN
cord-283109-ka3n9pft	18	15	kits	kit	NNS
cord-283109-ka3n9pft	18	16	needed	need	VBN
cord-283109-ka3n9pft	18	17	for	for	IN
cord-283109-ka3n9pft	18	18	the	the	DT
cord-283109-ka3n9pft	18	19	Centers	Centers	NNPS
cord-283109-ka3n9pft	18	20	for	for	IN
cord-283109-ka3n9pft	18	21	Disease	Disease	NNP
cord-283109-ka3n9pft	18	22	Control	Control	NNP
cord-283109-ka3n9pft	18	23	and	and	CC
cord-283109-ka3n9pft	18	24	Prevention	Prevention	NNP
cord-283109-ka3n9pft	18	25	(	(	-LRB-
cord-283109-ka3n9pft	18	26	CDC	CDC	NNP
cord-283109-ka3n9pft	18	27	)	)	-RRB-
cord-283109-ka3n9pft	18	28	RT	RT	NNP
cord-283109-ka3n9pft	18	29	-	-	HYPH
cord-283109-ka3n9pft	18	30	qPCR	qpcr	NN
cord-283109-ka3n9pft	18	31	assay	assay	NN
cord-283109-ka3n9pft	18	32	to	to	TO
cord-283109-ka3n9pft	18	33	diagnose	diagnose	VB
cord-283109-ka3n9pft	18	34	SARS	SARS	NNP
cord-283109-ka3n9pft	18	35	-	-	HYPH
cord-283109-ka3n9pft	18	36	CoV-2	CoV-2	NNP
cord-283109-ka3n9pft	18	37	.	.	.
cord-283109-ka3n9pft	19	1	During	during	IN
cord-283109-ka3n9pft	19	2	a	a	DT
cord-283109-ka3n9pft	19	3	study	study	NN
cord-283109-ka3n9pft	19	4	in	in	IN
cord-283109-ka3n9pft	19	5	developing	develop	VBG
cord-283109-ka3n9pft	19	6	a	a	DT
cord-283109-ka3n9pft	19	7	rapid	rapid	JJ
cord-283109-ka3n9pft	19	8	protocol	protocol	NN
cord-283109-ka3n9pft	19	9	for	for	IN
cord-283109-ka3n9pft	19	10	influenza	influenza	NN
cord-283109-ka3n9pft	19	11	(	(	-LRB-
cord-283109-ka3n9pft	19	12	Inf	Inf	NNP
cord-283109-ka3n9pft	19	13	)	)	-RRB-
cord-283109-ka3n9pft	19	14	and	and	CC
cord-283109-ka3n9pft	19	15	respiratory	respiratory	JJ
cord-283109-ka3n9pft	19	16	syncytial	syncytial	JJ
cord-283109-ka3n9pft	19	17	virus	virus	NN
cord-283109-ka3n9pft	19	18	(	(	-LRB-
cord-283109-ka3n9pft	19	19	RSV	RSV	NNP
cord-283109-ka3n9pft	19	20	)	)	-RRB-
cord-283109-ka3n9pft	19	21	diagnostics	diagnostic	NNS
cord-283109-ka3n9pft	19	22	,	,	,
cord-283109-ka3n9pft	19	23	we	-PRON-	PRP
cord-283109-ka3n9pft	19	24	investigated	investigate	VBD
cord-283109-ka3n9pft	19	25	the	the	DT
cord-283109-ka3n9pft	19	26	feasibility	feasibility	NN
cord-283109-ka3n9pft	19	27	of	of	IN
cord-283109-ka3n9pft	19	28	omitting	omit	VBG
cord-283109-ka3n9pft	19	29	the	the	DT
cord-283109-ka3n9pft	19	30	sample	sample	NN
cord-283109-ka3n9pft	19	31	preparation	preparation	NN
cord-283109-ka3n9pft	19	32	steps	step	NNS
cord-283109-ka3n9pft	19	33	to	to	TO
cord-283109-ka3n9pft	19	34	expedite	expedite	VB
cord-283109-ka3n9pft	19	35	the	the	DT
cord-283109-ka3n9pft	19	36	test	test	NN
cord-283109-ka3n9pft	19	37	without	without	IN
cord-283109-ka3n9pft	19	38	significantly	significantly	RB
cord-283109-ka3n9pft	19	39	impacting	impact	VBG
cord-283109-ka3n9pft	19	40	the	the	DT
cord-283109-ka3n9pft	19	41	test	test	NN
cord-283109-ka3n9pft	19	42	's	's	POS
cord-283109-ka3n9pft	19	43	sensitivity	sensitivity	NN
cord-283109-ka3n9pft	19	44	.	.	.
cord-283109-ka3n9pft	20	1	Using	use	VBG
cord-283109-ka3n9pft	20	2	Inf	Inf	NNP
cord-283109-ka3n9pft	20	3	and	and	CC
cord-283109-ka3n9pft	20	4	RSV	RSV	NNP
cord-283109-ka3n9pft	20	5	clinical	clinical	JJ
cord-283109-ka3n9pft	20	6	specimens	specimen	NNS
cord-283109-ka3n9pft	20	7	,	,	,
cord-283109-ka3n9pft	20	8	we	-PRON-	PRP
cord-283109-ka3n9pft	20	9	successfully	successfully	RB
cord-283109-ka3n9pft	20	10	performed	perform	VBD
cord-283109-ka3n9pft	20	11	RT	RT	NNP
cord-283109-ka3n9pft	20	12	-	-	HYPH
cord-283109-ka3n9pft	20	13	qPCR	qpcr	NN
cord-283109-ka3n9pft	20	14	reactions	reaction	NNS
cord-283109-ka3n9pft	20	15	by	by	IN
cord-283109-ka3n9pft	20	16	simply	simply	RB
cord-283109-ka3n9pft	20	17	adding	add	VBG
cord-283109-ka3n9pft	20	18	a	a	DT
cord-283109-ka3n9pft	20	19	few	few	JJ
cord-283109-ka3n9pft	20	20	microliters	microliter	NNS
cord-283109-ka3n9pft	20	21	of	of	IN
cord-283109-ka3n9pft	20	22	the	the	DT
cord-283109-ka3n9pft	20	23	unprocessed	unprocessed	JJ
cord-283109-ka3n9pft	20	24	sample	sample	NN
cord-283109-ka3n9pft	20	25	in	in	IN
cord-283109-ka3n9pft	20	26	viral	viral	JJ
cord-283109-ka3n9pft	20	27	transport	transport	NN
cord-283109-ka3n9pft	20	28	medium	medium	NN
cord-283109-ka3n9pft	20	29	(	(	-LRB-
cord-283109-ka3n9pft	20	30	VTM	VTM	NNP
cord-283109-ka3n9pft	20	31	)	)	-RRB-
cord-283109-ka3n9pft	20	32	directly	directly	RB
cord-283109-ka3n9pft	20	33	into	into	IN
cord-283109-ka3n9pft	20	34	the	the	DT
cord-283109-ka3n9pft	20	35	RT	RT	NNP
cord-283109-ka3n9pft	20	36	-	-	HYPH
cord-283109-ka3n9pft	20	37	qPCR	qpcr	NN
cord-283109-ka3n9pft	20	38	assay	assay	NN
cord-283109-ka3n9pft	20	39	master	master	NN
cord-283109-ka3n9pft	20	40	mix	mix	NN
cord-283109-ka3n9pft	20	41	.	.	.
cord-283109-ka3n9pft	21	1	We	-PRON-	PRP
cord-283109-ka3n9pft	21	2	then	then	RB
cord-283109-ka3n9pft	21	3	tested	test	VBD
cord-283109-ka3n9pft	21	4	the	the	DT
cord-283109-ka3n9pft	21	5	approach	approach	NN
cord-283109-ka3n9pft	21	6	using	use	VBG
cord-283109-ka3n9pft	21	7	SARS	SARS	NNP
cord-283109-ka3n9pft	21	8	-	-	HYPH
cord-283109-ka3n9pft	21	9	CoV-2	CoV-2	NNP
cord-283109-ka3n9pft	21	10	plasmid	plasmid	NN
cord-283109-ka3n9pft	21	11	and	and	CC
cord-283109-ka3n9pft	21	12	SeraCare	SeraCare	NNP
cord-283109-ka3n9pft	21	13	AccuPlex	AccuPlex	NNP
cord-283109-ka3n9pft	21	14	reference	reference	NN
cord-283109-ka3n9pft	21	15	materials	material	NNS
cord-283109-ka3n9pft	21	16	.	.	.
cord-283109-ka3n9pft	22	1	The	the	DT
cord-283109-ka3n9pft	22	2	data	datum	NNS
cord-283109-ka3n9pft	22	3	presented	present	VBN
cord-283109-ka3n9pft	22	4	below	below	RB
cord-283109-ka3n9pft	22	5	suggest	suggest	VBP
cord-283109-ka3n9pft	22	6	that	that	IN
cord-283109-ka3n9pft	22	7	it	-PRON-	PRP
cord-283109-ka3n9pft	22	8	is	be	VBZ
cord-283109-ka3n9pft	22	9	possible	possible	JJ
cord-283109-ka3n9pft	22	10	to	to	TO
cord-283109-ka3n9pft	22	11	skip	skip	VB
cord-283109-ka3n9pft	22	12	the	the	DT
cord-283109-ka3n9pft	22	13	RNA	RNA	NNP
cord-283109-ka3n9pft	22	14	extraction	extraction	NN
cord-283109-ka3n9pft	22	15	step	step	NN
cord-283109-ka3n9pft	22	16	in	in	IN
cord-283109-ka3n9pft	22	17	COVID-19	COVID-19	NNP
cord-283109-ka3n9pft	22	18	testing	testing	NN
cord-283109-ka3n9pft	22	19	without	without	IN
cord-283109-ka3n9pft	22	20	a	a	DT
cord-283109-ka3n9pft	22	21	significant	significant	JJ
cord-283109-ka3n9pft	22	22	drop	drop	NN
cord-283109-ka3n9pft	22	23	in	in	IN
cord-283109-ka3n9pft	22	24	assay	assay	NN
cord-283109-ka3n9pft	22	25	sensitivity	sensitivity	NN
cord-283109-ka3n9pft	22	26	.	.	.
cord-283109-ka3n9pft	23	1	We	-PRON-	PRP
cord-283109-ka3n9pft	23	2	first	first	RB
cord-283109-ka3n9pft	23	3	tested	test	VBD
cord-283109-ka3n9pft	23	4	the	the	DT
cord-283109-ka3n9pft	23	5	feasibility	feasibility	NN
cord-283109-ka3n9pft	23	6	using	use	VBG
cord-283109-ka3n9pft	23	7	a	a	DT
cord-283109-ka3n9pft	23	8	very	very	RB
cord-283109-ka3n9pft	23	9	small	small	JJ
cord-283109-ka3n9pft	23	10	amount	amount	NN
cord-283109-ka3n9pft	23	11	of	of	IN
cord-283109-ka3n9pft	23	12	sample	sample	NN
cord-283109-ka3n9pft	23	13	in	in	IN
cord-283109-ka3n9pft	23	14	RT	RT	NNP
cord-283109-ka3n9pft	23	15	-	-	HYPH
cord-283109-ka3n9pft	23	16	qPCR	qpcr	NN
cord-283109-ka3n9pft	23	17	reactions	reaction	NNS
cord-283109-ka3n9pft	23	18	by	by	IN
cord-283109-ka3n9pft	23	19	using	use	VBG
cord-283109-ka3n9pft	23	20	aerosol	aerosol	NN
cord-283109-ka3n9pft	23	21	generating	generate	VBG
cord-283109-ka3n9pft	23	22	vials	vial	NNS
cord-283109-ka3n9pft	23	23	to	to	TO
cord-283109-ka3n9pft	23	24	spray	spray	VB
cord-283109-ka3n9pft	23	25	the	the	DT
cord-283109-ka3n9pft	23	26	samples	sample	NNS
cord-283109-ka3n9pft	23	27	over	over	IN
cord-283109-ka3n9pft	23	28	the	the	DT
cord-283109-ka3n9pft	23	29	uncapped	uncapped	JJ
cord-283109-ka3n9pft	23	30	PCR	PCR	NNP
cord-283109-ka3n9pft	23	31	tubes	tube	NNS
cord-283109-ka3n9pft	23	32	.	.	.
cord-283109-ka3n9pft	24	1	The	the	DT
cord-283109-ka3n9pft	24	2	material	material	NN
cord-283109-ka3n9pft	24	3	in	in	IN
cord-283109-ka3n9pft	24	4	vials	vial	NNS
cord-283109-ka3n9pft	24	5	containing	contain	VBG
cord-283109-ka3n9pft	24	6	influenza	influenza	NN
cord-283109-ka3n9pft	24	7	A	a	NN
cord-283109-ka3n9pft	24	8	(	(	-LRB-
cord-283109-ka3n9pft	24	9	InfA	InfA	NNP
cord-283109-ka3n9pft	24	10	)	)	-RRB-
cord-283109-ka3n9pft	24	11	,	,	,
cord-283109-ka3n9pft	24	12	influenza	influenza	NN
cord-283109-ka3n9pft	24	13	B	b	NN
cord-283109-ka3n9pft	24	14	(	(	-LRB-
cord-283109-ka3n9pft	24	15	InfB	InfB	NNP
cord-283109-ka3n9pft	24	16	)	)	-RRB-
cord-283109-ka3n9pft	24	17	,	,	,
cord-283109-ka3n9pft	24	18	or	or	CC
cord-283109-ka3n9pft	24	19	RSV	RSV	NNP
cord-283109-ka3n9pft	24	20	clinical	clinical	JJ
cord-283109-ka3n9pft	24	21	specimens	specimen	NNS
cord-283109-ka3n9pft	24	22	(	(	-LRB-
cord-283109-ka3n9pft	24	23	swabs	swab	NNS
cord-283109-ka3n9pft	24	24	in	in	IN
cord-283109-ka3n9pft	24	25	VTM	VTM	NNP
cord-283109-ka3n9pft	24	26	)	)	-RRB-
cord-283109-ka3n9pft	24	27	were	be	VBD
cord-283109-ka3n9pft	24	28	sprayed	spray	VBN
cord-283109-ka3n9pft	24	29	into	into	IN
cord-283109-ka3n9pft	24	30	PCR	PCR	NNP
cord-283109-ka3n9pft	24	31	tubes	tube	NNS
cord-283109-ka3n9pft	24	32	containing	contain	VBG
cord-283109-ka3n9pft	24	33	primer	primer	NN
cord-283109-ka3n9pft	24	34	and	and	CC
cord-283109-ka3n9pft	24	35	probe	probe	NN
cord-283109-ka3n9pft	24	36	sets	set	NNS
cord-283109-ka3n9pft	24	37	targeting	target	VBG
cord-283109-ka3n9pft	24	38	InfA	InfA	NNP
cord-283109-ka3n9pft	24	39	,	,	,
cord-283109-ka3n9pft	24	40	InfB	InfB	NNP
cord-283109-ka3n9pft	24	41	,	,	,
cord-283109-ka3n9pft	24	42	RSV	RSV	NNP
cord-283109-ka3n9pft	24	43	and	and	CC
cord-283109-ka3n9pft	24	44	RNaseP	RNaseP	NNP
cord-283109-ka3n9pft	24	45	(	(	-LRB-
cord-283109-ka3n9pft	24	46	RP	RP	NNP
cord-283109-ka3n9pft	24	47	)	)	-RRB-
cord-283109-ka3n9pft	24	48	in	in	IN
cord-283109-ka3n9pft	24	49	master	master	NN
cord-283109-ka3n9pft	24	50	mix	mix	NN
cord-283109-ka3n9pft	24	51	prior	prior	RB
cord-283109-ka3n9pft	24	52	to	to	IN
cord-283109-ka3n9pft	24	53	capping	cap	VBG
cord-283109-ka3n9pft	24	54	the	the	DT
cord-283109-ka3n9pft	24	55	tubes	tube	NNS
cord-283109-ka3n9pft	24	56	and	and	CC
cord-283109-ka3n9pft	24	57	performing	perform	VBG
cord-283109-ka3n9pft	24	58	RT	rt	NN
cord-283109-ka3n9pft	24	59	-	-	HYPH
cord-283109-ka3n9pft	24	60	qPCR	qPCR	NNP
cord-283109-ka3n9pft	24	61	.	.	.
cord-283109-ka3n9pft	25	1	Fig	Fig	NNP
cord-283109-ka3n9pft	25	2	.	.	NNP
cord-283109-ka3n9pft	25	3	1	1	CD
cord-283109-ka3n9pft	25	4	shows	show	VBZ
cord-283109-ka3n9pft	25	5	that	that	IN
cord-283109-ka3n9pft	25	6	there	there	EX
cord-283109-ka3n9pft	25	7	were	be	VBD
cord-283109-ka3n9pft	25	8	positive	positive	JJ
cord-283109-ka3n9pft	25	9	PCR	PCR	NNP
cord-283109-ka3n9pft	25	10	signals	signal	NNS
cord-283109-ka3n9pft	25	11	in	in	IN
cord-283109-ka3n9pft	25	12	the	the	DT
cord-283109-ka3n9pft	25	13	respective	respective	JJ
cord-283109-ka3n9pft	25	14	tubes	tube	NNS
cord-283109-ka3n9pft	25	15	that	that	WDT
cord-283109-ka3n9pft	25	16	did	do	VBD
cord-283109-ka3n9pft	25	17	not	not	RB
cord-283109-ka3n9pft	25	18	have	have	VB
cord-283109-ka3n9pft	25	19	any	any	DT
cord-283109-ka3n9pft	25	20	non	non	JJ
cord-283109-ka3n9pft	25	21	-	-	JJ
cord-283109-ka3n9pft	25	22	specific	specific	JJ
cord-283109-ka3n9pft	25	23	amplification	amplification	NN
cord-283109-ka3n9pft	25	24	.	.	.
cord-283109-ka3n9pft	26	1	Though	though	IN
cord-283109-ka3n9pft	26	2	the	the	DT
cord-283109-ka3n9pft	26	3	unprocessed	unprocessed	JJ
cord-283109-ka3n9pft	26	4	,	,	,
cord-283109-ka3n9pft	26	5	sprayed	spray	VBN
cord-283109-ka3n9pft	26	6	samples	sample	NNS
cord-283109-ka3n9pft	26	7	have	have	VBP
cord-283109-ka3n9pft	26	8	higher	high	JJR
cord-283109-ka3n9pft	26	9	Ct	ct	JJ
cord-283109-ka3n9pft	26	10	values	value	NNS
cord-283109-ka3n9pft	26	11	(	(	-LRB-
cord-283109-ka3n9pft	26	12	36.5	36.5	CD
cord-283109-ka3n9pft	26	13	,	,	,
cord-283109-ka3n9pft	26	14	36.2	36.2	CD
cord-283109-ka3n9pft	26	15	and	and	CC
cord-283109-ka3n9pft	26	16	31	31	CD
cord-283109-ka3n9pft	26	17	for	for	IN
cord-283109-ka3n9pft	26	18	InfA	InfA	NNP
cord-283109-ka3n9pft	26	19	,	,	,
cord-283109-ka3n9pft	26	20	InfB	InfB	NNP
cord-283109-ka3n9pft	26	21	,	,	,
cord-283109-ka3n9pft	26	22	and	and	CC
cord-283109-ka3n9pft	26	23	RSV	RSV	NNP
cord-283109-ka3n9pft	26	24	,	,	,
cord-283109-ka3n9pft	26	25	respectively	respectively	RB
cord-283109-ka3n9pft	26	26	)	)	-RRB-
cord-283109-ka3n9pft	26	27	than	than	IN
cord-283109-ka3n9pft	26	28	the	the	DT
cord-283109-ka3n9pft	26	29	corresponding	correspond	VBG
cord-283109-ka3n9pft	26	30	extracted	extract	VBN
cord-283109-ka3n9pft	26	31	RNA	rna	NN
cord-283109-ka3n9pft	26	32	template	template	NN
cord-283109-ka3n9pft	26	33	samples	sample	NNS
cord-283109-ka3n9pft	26	34	(	(	-LRB-
cord-283109-ka3n9pft	26	35	30.4	30.4	CD
cord-283109-ka3n9pft	26	36	,	,	,
cord-283109-ka3n9pft	26	37	32.6	32.6	CD
cord-283109-ka3n9pft	26	38	,	,	,
cord-283109-ka3n9pft	26	39	29.6	29.6	CD
cord-283109-ka3n9pft	26	40	for	for	IN
cord-283109-ka3n9pft	26	41	InfA	InfA	NNP
cord-283109-ka3n9pft	26	42	,	,	,
cord-283109-ka3n9pft	26	43	InfB	InfB	NNP
cord-283109-ka3n9pft	26	44	,	,	,
cord-283109-ka3n9pft	26	45	and	and	CC
cord-283109-ka3n9pft	26	46	RSV	RSV	NNP
cord-283109-ka3n9pft	26	47	)	)	-RRB-
cord-283109-ka3n9pft	26	48	.	.	.
cord-283109-ka3n9pft	27	1	It	-PRON-	PRP
cord-283109-ka3n9pft	27	2	is	be	VBZ
cord-283109-ka3n9pft	27	3	important	important	JJ
cord-283109-ka3n9pft	27	4	to	to	TO
cord-283109-ka3n9pft	27	5	note	note	VB
cord-283109-ka3n9pft	27	6	that	that	IN
cord-283109-ka3n9pft	27	7	we	-PRON-	PRP
cord-283109-ka3n9pft	27	8	were	be	VBD
cord-283109-ka3n9pft	27	9	able	able	JJ
cord-283109-ka3n9pft	27	10	to	to	TO
cord-283109-ka3n9pft	27	11	detect	detect	VB
cord-283109-ka3n9pft	27	12	low	low	JJ
cord-283109-ka3n9pft	27	13	viral	viral	JJ
cord-283109-ka3n9pft	27	14	load	load	NN
cord-283109-ka3n9pft	27	15	samples	sample	NNS
cord-283109-ka3n9pft	27	16	(	(	-LRB-
cord-283109-ka3n9pft	27	17	Ct	Ct	NNP
cord-283109-ka3n9pft	27	18	>	>	XX
cord-283109-ka3n9pft	27	19	30	30	CD
cord-283109-ka3n9pft	27	20	)	)	-RRB-
cord-283109-ka3n9pft	27	21	when	when	WRB
cord-283109-ka3n9pft	27	22	less	less	JJR
cord-283109-ka3n9pft	27	23	than	than	IN
cord-283109-ka3n9pft	27	24	1	1	CD
cord-283109-ka3n9pft	27	25	µL	µl	CD
cord-283109-ka3n9pft	27	26	of	of	IN
cord-283109-ka3n9pft	27	27	sample	sample	NN
cord-283109-ka3n9pft	27	28	entered	enter	VBD
cord-283109-ka3n9pft	27	29	the	the	DT
cord-283109-ka3n9pft	27	30	tubes	tube	NNS
cord-283109-ka3n9pft	27	31	as	as	IN
cord-283109-ka3n9pft	27	32	measured	measure	VBN
cord-283109-ka3n9pft	27	33	by	by	IN
cord-283109-ka3n9pft	27	34	an	an	DT
cord-283109-ka3n9pft	27	35	analytical	analytical	JJ
cord-283109-ka3n9pft	27	36	balance	balance	NN
cord-283109-ka3n9pft	27	37	.	.	.
cord-283109-ka3n9pft	28	1	To	to	TO
cord-283109-ka3n9pft	28	2	test	test	VB
cord-283109-ka3n9pft	28	3	the	the	DT
cord-283109-ka3n9pft	28	4	extent	extent	NN
cord-283109-ka3n9pft	28	5	of	of	IN
cord-283109-ka3n9pft	28	6	PCR	PCR	NNP
cord-283109-ka3n9pft	28	7	inhibition	inhibition	NN
cord-283109-ka3n9pft	28	8	exerted	exert	VBN
cord-283109-ka3n9pft	28	9	by	by	IN
cord-283109-ka3n9pft	28	10	flu	flu	NN
cord-283109-ka3n9pft	28	11	specimens	specimen	NNS
cord-283109-ka3n9pft	28	12	in	in	IN
cord-283109-ka3n9pft	28	13	VTM	VTM	NNP
cord-283109-ka3n9pft	28	14	,	,	,
cord-283109-ka3n9pft	28	15	we	-PRON-	PRP
cord-283109-ka3n9pft	28	16	used	use	VBD
cord-283109-ka3n9pft	28	17	clinical	clinical	JJ
cord-283109-ka3n9pft	28	18	samples	sample	NNS
cord-283109-ka3n9pft	28	19	from	from	IN
cord-283109-ka3n9pft	28	20	Discovery	Discovery	NNP
cord-283109-ka3n9pft	28	21	Life	Life	NNP
cord-283109-ka3n9pft	28	22	Sciences	Sciences	NNPS
cord-283109-ka3n9pft	28	23	,	,	,
cord-283109-ka3n9pft	28	24	a	a	DT
cord-283109-ka3n9pft	28	25	Biospecimen	Biospecimen	NNP
cord-283109-ka3n9pft	28	26	repository	repository	NN
cord-283109-ka3n9pft	28	27	.	.	.
cord-283109-ka3n9pft	29	1	Between	between	IN
cord-283109-ka3n9pft	29	2	0.1	0.1	CD
cord-283109-ka3n9pft	29	3	µL	µl	CD
cord-283109-ka3n9pft	29	4	to	to	IN
cord-283109-ka3n9pft	29	5	6	6	CD
cord-283109-ka3n9pft	29	6	µL	µl	CD
cord-283109-ka3n9pft	29	7	of	of	IN
cord-283109-ka3n9pft	29	8	the	the	DT
cord-283109-ka3n9pft	29	9	unprocessed	unprocessed	JJ
cord-283109-ka3n9pft	29	10	samples	sample	NNS
cord-283109-ka3n9pft	29	11	were	be	VBD
cord-283109-ka3n9pft	29	12	directly	directly	RB
cord-283109-ka3n9pft	29	13	spiked	spike	VBN
cord-283109-ka3n9pft	29	14	into	into	IN
cord-283109-ka3n9pft	29	15	the	the	DT
cord-283109-ka3n9pft	29	16	master	master	NN
cord-283109-ka3n9pft	29	17	mix	mix	NN
cord-283109-ka3n9pft	29	18	with	with	IN
cord-283109-ka3n9pft	29	19	InfA	InfA	NNP
cord-283109-ka3n9pft	29	20	primers	primer	NNS
cord-283109-ka3n9pft	29	21	in	in	IN
cord-283109-ka3n9pft	29	22	a	a	DT
cord-283109-ka3n9pft	29	23	20	20	CD
cord-283109-ka3n9pft	29	24	µL	µl	IN
cord-283109-ka3n9pft	29	25	reaction	reaction	NN
cord-283109-ka3n9pft	29	26	mix	mix	NN
cord-283109-ka3n9pft	29	27	(	(	-LRB-
cord-283109-ka3n9pft	29	28	Fig	fig	NN
cord-283109-ka3n9pft	29	29	.	.	NNP
cord-283109-ka3n9pft	29	30	2	2	CD
cord-283109-ka3n9pft	29	31	)	)	-RRB-
cord-283109-ka3n9pft	29	32	.	.	.
cord-283109-ka3n9pft	30	1	An	an	DT
cord-283109-ka3n9pft	30	2	extracted	extract	VBN
cord-283109-ka3n9pft	30	3	template	template	NN
cord-283109-ka3n9pft	30	4	(	(	-LRB-
cord-283109-ka3n9pft	30	5	in	in	IN
cord-283109-ka3n9pft	30	6	Promega	Promega	NNP
cord-283109-ka3n9pft	30	7	Maxwell	Maxwell	NNP
cord-283109-ka3n9pft	30	8	device	device	NN
cord-283109-ka3n9pft	30	9	)	)	-RRB-
cord-283109-ka3n9pft	30	10	from	from	IN
cord-283109-ka3n9pft	30	11	the	the	DT
cord-283109-ka3n9pft	30	12	same	same	JJ
cord-283109-ka3n9pft	30	13	sample	sample	NN
cord-283109-ka3n9pft	30	14	was	be	VBD
cord-283109-ka3n9pft	30	15	also	also	RB
cord-283109-ka3n9pft	30	16	amplified	amplify	VBN
cord-283109-ka3n9pft	30	17	along	along	RP
cord-283109-ka3n9pft	30	18	with	with	IN
cord-283109-ka3n9pft	30	19	unprocessed	unprocessed	JJ
cord-283109-ka3n9pft	30	20	samples	sample	NNS
cord-283109-ka3n9pft	30	21	.	.	.
cord-283109-ka3n9pft	31	1	Adding	add	VBG
cord-283109-ka3n9pft	31	2	more	more	JJR
cord-283109-ka3n9pft	31	3	unprocessed	unprocessed	JJ
cord-283109-ka3n9pft	31	4	samples	sample	NNS
cord-283109-ka3n9pft	31	5	(	(	-LRB-
cord-283109-ka3n9pft	31	6	up	up	IN
cord-283109-ka3n9pft	31	7	to	to	TO
cord-283109-ka3n9pft	31	8	6	6	CD
cord-283109-ka3n9pft	31	9	µL	µl	CD
cord-283109-ka3n9pft	31	10	)	)	-RRB-
cord-283109-ka3n9pft	31	11	improved	improve	VBD
cord-283109-ka3n9pft	31	12	(	(	-LRB-
cord-283109-ka3n9pft	31	13	reduced	reduce	VBN
cord-283109-ka3n9pft	31	14	)	)	-RRB-
cord-283109-ka3n9pft	31	15	the	the	DT
cord-283109-ka3n9pft	31	16	Ct	ct	JJ
cord-283109-ka3n9pft	31	17	values	value	NNS
cord-283109-ka3n9pft	31	18	.	.	.
cord-283109-ka3n9pft	32	1	The	the	DT
cord-283109-ka3n9pft	32	2	Ct	Ct	NNP
cord-283109-ka3n9pft	32	3	difference	difference	NN
cord-283109-ka3n9pft	32	4	between	between	IN
cord-283109-ka3n9pft	32	5	the	the	DT
cord-283109-ka3n9pft	32	6	extracted	extract	VBN
cord-283109-ka3n9pft	32	7	template	template	NN
cord-283109-ka3n9pft	32	8	(	(	-LRB-
cord-283109-ka3n9pft	32	9	100	100	CD
cord-283109-ka3n9pft	32	10	µL	µl	CD
cord-283109-ka3n9pft	32	11	input	input	NN
cord-283109-ka3n9pft	32	12	and	and	CC
cord-283109-ka3n9pft	32	13	100	100	CD
cord-283109-ka3n9pft	32	14	µL	µl	IN
cord-283109-ka3n9pft	32	15	eluate	eluate	NN
cord-283109-ka3n9pft	32	16	)	)	-RRB-
cord-283109-ka3n9pft	32	17	and	and	CC
cord-283109-ka3n9pft	32	18	the	the	DT
cord-283109-ka3n9pft	32	19	unprocessed	unprocessed	JJ
cord-283109-ka3n9pft	32	20	sample	sample	NN
cord-283109-ka3n9pft	32	21	is	be	VBZ
cord-283109-ka3n9pft	32	22	minimal	minimal	JJ
cord-283109-ka3n9pft	32	23	(	(	-LRB-
cord-283109-ka3n9pft	32	24	29.3	29.3	CD
cord-283109-ka3n9pft	32	25	vs.	vs.	IN
cord-283109-ka3n9pft	32	26	30.3	30.3	CD
cord-283109-ka3n9pft	32	27	,	,	,
cord-283109-ka3n9pft	32	28	respectively	respectively	RB
cord-283109-ka3n9pft	32	29	)	)	-RRB-
cord-283109-ka3n9pft	32	30	.	.	.
cord-283109-ka3n9pft	33	1	Adding	add	VBG
cord-283109-ka3n9pft	33	2	more	more	JJR
cord-283109-ka3n9pft	33	3	than	than	IN
cord-283109-ka3n9pft	33	4	6	6	CD
cord-283109-ka3n9pft	33	5	µL	µl	CD
cord-283109-ka3n9pft	33	6	of	of	IN
cord-283109-ka3n9pft	33	7	untreated	untreated	JJ
cord-283109-ka3n9pft	33	8	samples	sample	NNS
cord-283109-ka3n9pft	33	9	has	have	VBZ
cord-283109-ka3n9pft	33	10	resulted	result	VBN
cord-283109-ka3n9pft	33	11	in	in	IN
cord-283109-ka3n9pft	33	12	high	high	JJ
cord-283109-ka3n9pft	33	13	Ct	Ct	NNP
cord-283109-ka3n9pft	33	14	(	(	-LRB-
cord-283109-ka3n9pft	33	15	data	datum	NNS
cord-283109-ka3n9pft	33	16	not	not	RB
cord-283109-ka3n9pft	33	17	shown	show	VBN
cord-283109-ka3n9pft	33	18	)	)	-RRB-
cord-283109-ka3n9pft	33	19	,	,	,
cord-283109-ka3n9pft	33	20	meaning	mean	VBG
cord-283109-ka3n9pft	33	21	the	the	DT
cord-283109-ka3n9pft	33	22	inhibitory	inhibitory	JJ
cord-283109-ka3n9pft	33	23	effect	effect	NN
cord-283109-ka3n9pft	33	24	outweighed	outweigh	VBD
cord-283109-ka3n9pft	33	25	the	the	DT
cord-283109-ka3n9pft	33	26	benefits	benefit	NNS
cord-283109-ka3n9pft	33	27	of	of	IN
cord-283109-ka3n9pft	33	28	having	have	VBG
cord-283109-ka3n9pft	33	29	more	more	JJR
cord-283109-ka3n9pft	33	30	copies	copy	NNS
cord-283109-ka3n9pft	33	31	of	of	IN
cord-283109-ka3n9pft	33	32	the	the	DT
cord-283109-ka3n9pft	33	33	target	target	NN
cord-283109-ka3n9pft	33	34	in	in	IN
cord-283109-ka3n9pft	33	35	a	a	DT
cord-283109-ka3n9pft	33	36	reaction	reaction	NN
cord-283109-ka3n9pft	33	37	.	.	.
cord-283109-ka3n9pft	34	1	We	-PRON-	PRP
cord-283109-ka3n9pft	34	2	next	next	RB
cord-283109-ka3n9pft	34	3	tested	test	VBD
cord-283109-ka3n9pft	34	4	whether	whether	IN
cord-283109-ka3n9pft	34	5	the	the	DT
cord-283109-ka3n9pft	34	6	RNA	RNA	NNP
cord-283109-ka3n9pft	34	7	from	from	IN
cord-283109-ka3n9pft	34	8	SARS	SARS	NNP
cord-283109-ka3n9pft	34	9	-	-	HYPH
cord-283109-ka3n9pft	34	10	CoV-2	CoV-2	NNP
cord-283109-ka3n9pft	34	11	can	can	MD
cord-283109-ka3n9pft	34	12	be	be	VB
cord-283109-ka3n9pft	34	13	detected	detect	VBN
cord-283109-ka3n9pft	34	14	by	by	IN
cord-283109-ka3n9pft	34	15	directly	directly	RB
cord-283109-ka3n9pft	34	16	spiking	spike	VBG
cord-283109-ka3n9pft	34	17	samples	sample	NNS
cord-283109-ka3n9pft	34	18	of	of	IN
cord-283109-ka3n9pft	34	19	the	the	DT
cord-283109-ka3n9pft	34	20	non	non	JJ
cord-283109-ka3n9pft	34	21	-	-	JJ
cord-283109-ka3n9pft	34	22	replicative	replicative	JJ
cord-283109-ka3n9pft	34	23	recombinant	recombinant	JJ
cord-283109-ka3n9pft	34	24	virus	virus	NN
cord-283109-ka3n9pft	34	25	particles	particle	NNS
cord-283109-ka3n9pft	34	26	(	(	-LRB-
cord-283109-ka3n9pft	34	27	SeraCare	SeraCare	NNP
cord-283109-ka3n9pft	34	28	AccuPlex	AccuPlex	NNP
cord-283109-ka3n9pft	34	29	SARS	SARS	NNP
cord-283109-ka3n9pft	34	30	-	-	HYPH
cord-283109-ka3n9pft	34	31	CoV-2	CoV-2	NNP
cord-283109-ka3n9pft	34	32	reference	reference	NN
cord-283109-ka3n9pft	34	33	material	material	NN
cord-283109-ka3n9pft	34	34	)	)	-RRB-
cord-283109-ka3n9pft	34	35	in	in	IN
cord-283109-ka3n9pft	34	36	VTM	VTM	NNP
cord-283109-ka3n9pft	34	37	to	to	TO
cord-283109-ka3n9pft	34	38	master	master	NN
cord-283109-ka3n9pft	34	39	mix	mix	NN
cord-283109-ka3n9pft	34	40	without	without	IN
cord-283109-ka3n9pft	34	41	an	an	DT
cord-283109-ka3n9pft	34	42	extraction	extraction	NN
cord-283109-ka3n9pft	34	43	step	step	NN
cord-283109-ka3n9pft	34	44	.	.	.
cord-283109-ka3n9pft	35	1	The	the	DT
cord-283109-ka3n9pft	35	2	SARS	SARS	NNP
cord-283109-ka3n9pft	35	3	-	-	HYPH
cord-283109-ka3n9pft	35	4	CoV-2	CoV-2	NNP
cord-283109-ka3n9pft	35	5	virus	virus	NN
cord-283109-ka3n9pft	35	6	particles	particle	NNS
cord-283109-ka3n9pft	35	7	were	be	VBD
cord-283109-ka3n9pft	35	8	mixed	mix	VBN
cord-283109-ka3n9pft	35	9	with	with	IN
cord-283109-ka3n9pft	35	10	VTM	VTM	NNP
cord-283109-ka3n9pft	35	11	to	to	TO
cord-283109-ka3n9pft	35	12	get	get	VB
cord-283109-ka3n9pft	35	13	a	a	DT
cord-283109-ka3n9pft	35	14	final	final	JJ
cord-283109-ka3n9pft	35	15	concentration	concentration	NN
cord-283109-ka3n9pft	35	16	of	of	IN
cord-283109-ka3n9pft	35	17	2,500	2,500	CD
cord-283109-ka3n9pft	35	18	copies	copy	NNS
cord-283109-ka3n9pft	35	19	per	per	IN
cord-283109-ka3n9pft	35	20	mL.	mL.	NNP
cord-283109-ka3n9pft	35	21	Different	different	JJ
cord-283109-ka3n9pft	35	22	amounts	amount	NNS
cord-283109-ka3n9pft	35	23	(	(	-LRB-
cord-283109-ka3n9pft	35	24	2	2	CD
cord-283109-ka3n9pft	35	25	,	,	,
cord-283109-ka3n9pft	35	26	4	4	CD
cord-283109-ka3n9pft	35	27	,	,	,
cord-283109-ka3n9pft	35	28	6	6	CD
cord-283109-ka3n9pft	35	29	and	and	CC
cord-283109-ka3n9pft	35	30	8	8	CD
cord-283109-ka3n9pft	35	31	µL	µl	CD
cord-283109-ka3n9pft	35	32	)	)	-RRB-
cord-283109-ka3n9pft	35	33	of	of	IN
cord-283109-ka3n9pft	35	34	these	these	DT
cord-283109-ka3n9pft	35	35	mock	mock	JJ
cord-283109-ka3n9pft	35	36	clinical	clinical	JJ
cord-283109-ka3n9pft	35	37	samples	sample	NNS
cord-283109-ka3n9pft	35	38	were	be	VBD
cord-283109-ka3n9pft	35	39	spiked	spike	VBN
cord-283109-ka3n9pft	35	40	into	into	IN
cord-283109-ka3n9pft	35	41	the	the	DT
cord-283109-ka3n9pft	35	42	master	master	NN
cord-283109-ka3n9pft	35	43	mix	mix	NN
cord-283109-ka3n9pft	35	44	containing	contain	VBG
cord-283109-ka3n9pft	35	45	CDC	CDC	NNP
cord-283109-ka3n9pft	35	46	recommended	recommend	VBD
cord-283109-ka3n9pft	35	47	SARS	SARS	NNP
cord-283109-ka3n9pft	35	48	-	-	HYPH
cord-283109-ka3n9pft	35	49	CoV-2	CoV-2	NNP
cord-283109-ka3n9pft	35	50	RT	RT	NNP
cord-283109-ka3n9pft	35	51	-	-	HYPH
cord-283109-ka3n9pft	35	52	qPCR	qPCR	NNP
cord-283109-ka3n9pft	35	53	diagnostic	diagnostic	JJ
cord-283109-ka3n9pft	35	54	panel	panel	NN
cord-283109-ka3n9pft	35	55	primers	primer	NNS
cord-283109-ka3n9pft	35	56	N1	N1	NNP
cord-283109-ka3n9pft	35	57	,	,	,
cord-283109-ka3n9pft	35	58	N2	N2	NNP
cord-283109-ka3n9pft	35	59	o	o	NN
cord-283109-ka3n9pft	35	60	N3	n3	NN
cord-283109-ka3n9pft	35	61	in	in	IN
cord-283109-ka3n9pft	35	62	20	20	CD
cord-283109-ka3n9pft	35	63	µL	µl	XX
cord-283109-ka3n9pft	36	1	PCR	PCR	NNP
cord-283109-ka3n9pft	36	2	reactions	reaction	NNS
cord-283109-ka3n9pft	36	3	,	,	,
cord-283109-ka3n9pft	36	4	though	though	IN
cord-283109-ka3n9pft	36	5	we	-PRON-	PRP
cord-283109-ka3n9pft	36	6	note	note	VBP
cord-283109-ka3n9pft	36	7	that	that	IN
cord-283109-ka3n9pft	36	8	the	the	DT
cord-283109-ka3n9pft	36	9	N3	n3	NN
cord-283109-ka3n9pft	36	10	primers	primer	NNS
cord-283109-ka3n9pft	36	11	were	be	VBD
cord-283109-ka3n9pft	36	12	recently	recently	RB
cord-283109-ka3n9pft	36	13	removed	remove	VBN
cord-283109-ka3n9pft	36	14	by	by	IN
cord-283109-ka3n9pft	36	15	the	the	DT
cord-283109-ka3n9pft	36	16	CDC	CDC	NNP
cord-283109-ka3n9pft	36	17	.	.	.
cord-283109-ka3n9pft	37	1	Purified	purify	VBN
cord-283109-ka3n9pft	37	2	nucleic	nucleic	JJ
cord-283109-ka3n9pft	37	3	acid	acid	NN
cord-283109-ka3n9pft	37	4	template	template	NN
cord-283109-ka3n9pft	37	5	(	(	-LRB-
cord-283109-ka3n9pft	37	6	4	4	CD
cord-283109-ka3n9pft	37	7	µL	µl	CD
cord-283109-ka3n9pft	37	8	)	)	-RRB-
cord-283109-ka3n9pft	37	9	isolated	isolate	VBN
cord-283109-ka3n9pft	37	10	from	from	IN
cord-283109-ka3n9pft	37	11	a	a	DT
cord-283109-ka3n9pft	37	12	Promega	Promega	NNP
cord-283109-ka3n9pft	37	13	Maxwell	Maxwell	NNP
cord-283109-ka3n9pft	37	14	device	device	NN
cord-283109-ka3n9pft	37	15	was	be	VBD
cord-283109-ka3n9pft	37	16	also	also	RB
cord-283109-ka3n9pft	37	17	amplified	amplify	VBN
cord-283109-ka3n9pft	37	18	.	.	.
cord-283109-ka3n9pft	38	1	The	the	DT
cord-283109-ka3n9pft	38	2	Ct	Ct	NNP
cord-283109-ka3n9pft	38	3	difference	difference	NN
cord-283109-ka3n9pft	38	4	between	between	IN
cord-283109-ka3n9pft	38	5	the	the	DT
cord-283109-ka3n9pft	38	6	purified	purify	VBN
cord-283109-ka3n9pft	38	7	template	template	NN
cord-283109-ka3n9pft	38	8	and	and	CC
cord-283109-ka3n9pft	38	9	the	the	DT
cord-283109-ka3n9pft	38	10	directly	directly	RB
cord-283109-ka3n9pft	38	11	spiked	spike	VBN
cord-283109-ka3n9pft	38	12	clinical	clinical	JJ
cord-283109-ka3n9pft	38	13	samples	sample	NNS
cord-283109-ka3n9pft	38	14	(	(	-LRB-
cord-283109-ka3n9pft	38	15	i.e.	i.e.	FW
cord-283109-ka3n9pft	38	16	,	,	,
cord-283109-ka3n9pft	38	17	4	4	CD
cord-283109-ka3n9pft	38	18	µL	µl	CD
cord-283109-ka3n9pft	38	19	,	,	,
cord-283109-ka3n9pft	39	1	5	5	CD
cord-283109-ka3n9pft	39	2	µL	µl	CD
cord-283109-ka3n9pft	39	3	and	and	CC
cord-283109-ka3n9pft	39	4	6	6	CD
cord-283109-ka3n9pft	39	5	µL	µl	NN
cord-283109-ka3n9pft	39	6	)	)	-RRB-
cord-283109-ka3n9pft	39	7	is	be	VBZ
cord-283109-ka3n9pft	39	8	minimal	minimal	JJ
cord-283109-ka3n9pft	39	9	.	.	.
cord-283109-ka3n9pft	40	1	Then	then	RB
cord-283109-ka3n9pft	40	2	,	,	,
cord-283109-ka3n9pft	40	3	100	100	CD
cord-283109-ka3n9pft	40	4	µL	µl	CD
cord-283109-ka3n9pft	40	5	of	of	IN
cord-283109-ka3n9pft	40	6	AccuPlex	AccuPlex	NNP
cord-283109-ka3n9pft	40	7	SARS	SARS	NNP
cord-283109-ka3n9pft	40	8	-	-	HYPH
cord-283109-ka3n9pft	40	9	CoV-2	CoV-2	NNP
cord-283109-ka3n9pft	40	10	reference	reference	NN
cord-283109-ka3n9pft	40	11	material	material	NN
cord-283109-ka3n9pft	40	12	was	be	VBD
cord-283109-ka3n9pft	40	13	processed	process	VBN
cord-283109-ka3n9pft	40	14	in	in	IN
cord-283109-ka3n9pft	40	15	Promega	Promega	NNP
cord-283109-ka3n9pft	40	16	Maxwell	Maxwell	NNP
cord-283109-ka3n9pft	40	17	device	device	NN
cord-283109-ka3n9pft	40	18	(	(	-LRB-
cord-283109-ka3n9pft	40	19	using	use	VBG
cord-283109-ka3n9pft	40	20	an	an	DT
cord-283109-ka3n9pft	40	21	AS1520	AS1520	NNP
cord-283109-ka3n9pft	40	22	cartridge	cartridge	NN
cord-283109-ka3n9pft	40	23	)	)	-RRB-
cord-283109-ka3n9pft	40	24	and	and	CC
cord-283109-ka3n9pft	40	25	eluted	elute	VBN
cord-283109-ka3n9pft	40	26	in	in	IN
cord-283109-ka3n9pft	40	27	100	100	CD
cord-283109-ka3n9pft	40	28	µL	µl	CD
cord-283109-ka3n9pft	40	29	of	of	IN
cord-283109-ka3n9pft	40	30	elution	elution	NN
cord-283109-ka3n9pft	40	31	buffer	buffer	NN
cord-283109-ka3n9pft	40	32	.	.	.
cord-283109-ka3n9pft	41	1	The	the	DT
cord-283109-ka3n9pft	41	2	extracted	extract	VBN
cord-283109-ka3n9pft	41	3	template	template	NN
cord-283109-ka3n9pft	41	4	(	(	-LRB-
cord-283109-ka3n9pft	41	5	4	4	CD
cord-283109-ka3n9pft	41	6	µL	µL	NNP
cord-283109-ka3n9pft	41	7	)	)	-RRB-
cord-283109-ka3n9pft	41	8	was	be	VBD
cord-283109-ka3n9pft	41	9	added	add	VBN
cord-283109-ka3n9pft	41	10	to	to	TO
cord-283109-ka3n9pft	41	11	master	master	NN
cord-283109-ka3n9pft	41	12	mix	mix	NN
cord-283109-ka3n9pft	41	13	,	,	,
cord-283109-ka3n9pft	41	14	and	and	CC
cord-283109-ka3n9pft	41	15	RT	rt	NN
cord-283109-ka3n9pft	41	16	-	-	HYPH
cord-283109-ka3n9pft	41	17	qPCR	qpcr	NN
cord-283109-ka3n9pft	41	18	amplification	amplification	NN
cord-283109-ka3n9pft	41	19	was	be	VBD
cord-283109-ka3n9pft	41	20	carried	carry	VBN
cord-283109-ka3n9pft	41	21	out	out	RP
cord-283109-ka3n9pft	41	22	in	in	IN
cord-283109-ka3n9pft	41	23	a	a	DT
cord-283109-ka3n9pft	41	24	Bio	Bio	NNP
cord-283109-ka3n9pft	41	25	-	-	HYPH
cord-283109-ka3n9pft	41	26	Rad	Rad	NNP
cord-283109-ka3n9pft	41	27	(	(	-LRB-
cord-283109-ka3n9pft	41	28	CFX-96	CFX-96	NNP
cord-283109-ka3n9pft	41	29	)	)	-RRB-
cord-283109-ka3n9pft	41	30	thermal	thermal	JJ
cord-283109-ka3n9pft	41	31	cycler	cycler	NN
cord-283109-ka3n9pft	41	32	along	along	IN
cord-283109-ka3n9pft	41	33	with	with	IN
cord-283109-ka3n9pft	41	34	the	the	DT
cord-283109-ka3n9pft	41	35	reactions	reaction	NNS
cord-283109-ka3n9pft	41	36	with	with	IN
cord-283109-ka3n9pft	41	37	unprocessed	unprocessed	JJ
cord-283109-ka3n9pft	41	38	samples	sample	NNS
cord-283109-ka3n9pft	41	39	.	.	.
cord-283109-ka3n9pft	42	1	As	as	IN
cord-283109-ka3n9pft	42	2	shown	show	VBN
cord-283109-ka3n9pft	42	3	in	in	IN
cord-283109-ka3n9pft	42	4	Fig	Fig	NNP
cord-283109-ka3n9pft	42	5	.	.	.
cord-283109-ka3n9pft	43	1	3	3	LS
cord-283109-ka3n9pft	43	2	,	,	,
cord-283109-ka3n9pft	43	3	the	the	DT
cord-283109-ka3n9pft	43	4	SARS	SARS	NNP
cord-283109-ka3n9pft	43	5	-	-	HYPH
cord-283109-ka3n9pft	43	6	CoV-2	CoV-2	NNP
cord-283109-ka3n9pft	43	7	RNA	RNA	NNP
cord-283109-ka3n9pft	43	8	from	from	IN
cord-283109-ka3n9pft	43	9	directly	directly	RB
cord-283109-ka3n9pft	43	10	spiked	spiked	JJ
cord-283109-ka3n9pft	43	11	samples	sample	NNS
cord-283109-ka3n9pft	43	12	was	be	VBD
cord-283109-ka3n9pft	43	13	successfully	successfully	RB
cord-283109-ka3n9pft	43	14	detected	detect	VBN
cord-283109-ka3n9pft	43	15	by	by	IN
cord-283109-ka3n9pft	43	16	the	the	DT
cord-283109-ka3n9pft	43	17	RT	RT	NNP
cord-283109-ka3n9pft	43	18	-	-	HYPH
cord-283109-ka3n9pft	43	19	qPCR	qpcr	NN
cord-283109-ka3n9pft	43	20	reaction	reaction	NN
cord-283109-ka3n9pft	43	21	without	without	IN
cord-283109-ka3n9pft	43	22	a	a	DT
cord-283109-ka3n9pft	43	23	nucleic	nucleic	JJ
cord-283109-ka3n9pft	43	24	acid	acid	NN
cord-283109-ka3n9pft	43	25	extraction	extraction	NN
cord-283109-ka3n9pft	43	26	step	step	NN
cord-283109-ka3n9pft	43	27	(	(	-LRB-
cord-283109-ka3n9pft	43	28	N1	N1	NNP
cord-283109-ka3n9pft	43	29	target	target	NN
cord-283109-ka3n9pft	43	30	shown	show	VBN
cord-283109-ka3n9pft	43	31	)	)	-RRB-
cord-283109-ka3n9pft	43	32	.	.	.
cord-283109-ka3n9pft	44	1	Up	up	IN
cord-283109-ka3n9pft	44	2	to	to	TO
cord-283109-ka3n9pft	44	3	8	8	CD
cord-283109-ka3n9pft	44	4	µL	µl	CD
cord-283109-ka3n9pft	44	5	of	of	IN
cord-283109-ka3n9pft	44	6	VTM	VTM	NNP
cord-283109-ka3n9pft	44	7	and	and	CC
cord-283109-ka3n9pft	44	8	AccuPlex	AccuPlex	NNP
cord-283109-ka3n9pft	44	9	SARS	SARS	NNP
cord-283109-ka3n9pft	44	10	-	-	HYPH
cord-283109-ka3n9pft	44	11	CoV-2	CoV-2	NNP
cord-283109-ka3n9pft	44	12	mix	mix	NN
cord-283109-ka3n9pft	44	13	was	be	VBD
cord-283109-ka3n9pft	44	14	used	use	VBN
cord-283109-ka3n9pft	44	15	in	in	IN
cord-283109-ka3n9pft	44	16	the	the	DT
cord-283109-ka3n9pft	44	17	reaction	reaction	NN
cord-283109-ka3n9pft	44	18	,	,	,
cord-283109-ka3n9pft	44	19	which	which	WDT
cord-283109-ka3n9pft	44	20	did	do	VBD
cord-283109-ka3n9pft	44	21	not	not	RB
cord-283109-ka3n9pft	44	22	exert	exert	VB
cord-283109-ka3n9pft	44	23	major	major	JJ
cord-283109-ka3n9pft	44	24	template	template	NN
cord-283109-ka3n9pft	44	25	(	(	-LRB-
cord-283109-ka3n9pft	44	26	20	20	CD
cord-283109-ka3n9pft	44	27	copies	copy	NNS
cord-283109-ka3n9pft	44	28	/	/	SYM
cord-283109-ka3n9pft	44	29	reaction	reaction	NN
cord-283109-ka3n9pft	44	30	)	)	-RRB-
cord-283109-ka3n9pft	44	31	is	be	VBZ
cord-283109-ka3n9pft	44	32	35.5	35.5	CD
cord-283109-ka3n9pft	44	33	,	,	,
cord-283109-ka3n9pft	44	34	which	which	WDT
cord-283109-ka3n9pft	44	35	is	be	VBZ
cord-283109-ka3n9pft	44	36	2	2	CD
cord-283109-ka3n9pft	44	37	cycles	cycle	NNS
cord-283109-ka3n9pft	44	38	lower	low	JJR
cord-283109-ka3n9pft	44	39	than	than	IN
cord-283109-ka3n9pft	44	40	the	the	DT
cord-283109-ka3n9pft	44	41	result	result	NN
cord-283109-ka3n9pft	44	42	from	from	IN
cord-283109-ka3n9pft	44	43	4	4	CD
cord-283109-ka3n9pft	44	44	µL	µl	CD
cord-283109-ka3n9pft	44	45	of	of	IN
cord-283109-ka3n9pft	44	46	untreated	untreated	JJ
cord-283109-ka3n9pft	44	47	sample	sample	NN
cord-283109-ka3n9pft	44	48	(	(	-LRB-
cord-283109-ka3n9pft	44	49	10	10	CD
cord-283109-ka3n9pft	44	50	copies	copy	NNS
cord-283109-ka3n9pft	44	51	)	)	-RRB-
cord-283109-ka3n9pft	44	52	in	in	IN
cord-283109-ka3n9pft	44	53	the	the	DT
cord-283109-ka3n9pft	44	54	reaction	reaction	NN
cord-283109-ka3n9pft	44	55	(	(	-LRB-
cord-283109-ka3n9pft	44	56	Fig	Fig	NNP
cord-283109-ka3n9pft	44	57	.	.	.
cord-283109-ka3n9pft	44	58	3	3	CD
cord-283109-ka3n9pft	44	59	)	)	-RRB-
cord-283109-ka3n9pft	44	60	.	.	.
cord-283109-ka3n9pft	45	1	We	-PRON-	PRP
cord-283109-ka3n9pft	45	2	speculate	speculate	VBP
cord-283109-ka3n9pft	45	3	that	that	IN
cord-283109-ka3n9pft	45	4	the	the	DT
cord-283109-ka3n9pft	45	5	SeraCare	SeraCare	NNP
cord-283109-ka3n9pft	45	6	matrix	matrix	NN
cord-283109-ka3n9pft	45	7	,	,	,
cord-283109-ka3n9pft	45	8	containing	contain	VBG
cord-283109-ka3n9pft	45	9	Trisbuffered	Trisbuffered	NNP
cord-283109-ka3n9pft	45	10	saline	saline	NN
cord-283109-ka3n9pft	45	11	,	,	,
cord-283109-ka3n9pft	45	12	glycerol	glycerol	NN
cord-283109-ka3n9pft	45	13	,	,	,
cord-283109-ka3n9pft	45	14	anti	anti	JJ
cord-283109-ka3n9pft	45	15	-	-	JJ
cord-283109-ka3n9pft	45	16	microbial	microbial	JJ
cord-283109-ka3n9pft	45	17	agents	agent	NNS
cord-283109-ka3n9pft	45	18	and	and	CC
cord-283109-ka3n9pft	45	19	human	human	JJ
cord-283109-ka3n9pft	45	20	proteins	protein	NNS
cord-283109-ka3n9pft	45	21	,	,	,
cord-283109-ka3n9pft	45	22	could	could	MD
cord-283109-ka3n9pft	45	23	be	be	VB
cord-283109-ka3n9pft	45	24	the	the	DT
cord-283109-ka3n9pft	45	25	reason	reason	NN
cord-283109-ka3n9pft	45	26	for	for	IN
cord-283109-ka3n9pft	45	27	the	the	DT
cord-283109-ka3n9pft	45	28	above	above	RB
cord-283109-ka3n9pft	45	29	noticed	notice	VBN
cord-283109-ka3n9pft	45	30	PCR	PCR	NNP
cord-283109-ka3n9pft	45	31	inhibition	inhibition	NN
cord-283109-ka3n9pft	45	32	.	.	.
cord-283109-ka3n9pft	46	1	We	-PRON-	PRP
cord-283109-ka3n9pft	46	2	also	also	RB
cord-283109-ka3n9pft	46	3	tested	test	VBD
cord-283109-ka3n9pft	46	4	VTM	VTM	NNP
cord-283109-ka3n9pft	46	5	mixed	mix	VBN
cord-283109-ka3n9pft	46	6	SARS	SARS	NNP
cord-283109-ka3n9pft	46	7	-	-	HYPH
cord-283109-ka3n9pft	46	8	CoV-2	CoV-2	NNP
cord-283109-ka3n9pft	46	9	plasmid	plasmid	NN
cord-283109-ka3n9pft	46	10	(	(	-LRB-
cord-283109-ka3n9pft	46	11	purchased	purchase	VBN
cord-283109-ka3n9pft	46	12	from	from	IN
cord-283109-ka3n9pft	46	13	Integrated	Integrated	NNP
cord-283109-ka3n9pft	46	14	DNA	DNA	NNP
cord-283109-ka3n9pft	46	15	Technologies	Technologies	NNP
cord-283109-ka3n9pft	46	16	,	,	,
cord-283109-ka3n9pft	46	17	Inc.	Inc.	NNP
cord-283109-ka3n9pft	46	18	)	)	-RRB-
cord-283109-ka3n9pft	46	19	using	use	VBG
cord-283109-ka3n9pft	46	20	RT	RT	NNP
cord-283109-ka3n9pft	46	21	-	-	HYPH
cord-283109-ka3n9pft	46	22	qPCR	qPCR	NNP
cord-283109-ka3n9pft	46	23	.	.	.
cord-283109-ka3n9pft	47	1	This	this	DT
cord-283109-ka3n9pft	47	2	plasmid	plasmid	NN
cord-283109-ka3n9pft	47	3	is	be	VBZ
cord-283109-ka3n9pft	47	4	used	use	VBN
cord-283109-ka3n9pft	47	5	as	as	IN
cord-283109-ka3n9pft	47	6	a	a	DT
cord-283109-ka3n9pft	47	7	positive	positive	JJ
cord-283109-ka3n9pft	47	8	control	control	NN
cord-283109-ka3n9pft	47	9	for	for	IN
cord-283109-ka3n9pft	47	10	the	the	DT
cord-283109-ka3n9pft	47	11	CDC	CDC	NNP
cord-283109-ka3n9pft	47	12	's	's	POS
cord-283109-ka3n9pft	47	13	SARS	SARS	NNP
cord-283109-ka3n9pft	47	14	-	-	HYPH
cord-283109-ka3n9pft	47	15	CoV-2	CoV-2	NNP
cord-283109-ka3n9pft	47	16	RT	RT	NNP
cord-283109-ka3n9pft	47	17	-	-	HYPH
cord-283109-ka3n9pft	47	18	qPCR	qpcr	NN
cord-283109-ka3n9pft	47	19	assay	assay	NN
cord-283109-ka3n9pft	47	20	.	.	.
cord-283109-ka3n9pft	48	1	The	the	DT
cord-283109-ka3n9pft	48	2	positive	positive	JJ
cord-283109-ka3n9pft	48	3	control	control	NN
cord-283109-ka3n9pft	48	4	plasmid	plasmid	NN
cord-283109-ka3n9pft	48	5	was	be	VBD
cord-283109-ka3n9pft	48	6	mixed	mix	VBN
cord-283109-ka3n9pft	48	7	with	with	IN
cord-283109-ka3n9pft	48	8	VTM	VTM	NNP
cord-283109-ka3n9pft	48	9	and	and	CC
cord-283109-ka3n9pft	48	10	4	4	CD
cord-283109-ka3n9pft	48	11	µL	µl	CD
cord-283109-ka3n9pft	48	12	of	of	IN
cord-283109-ka3n9pft	48	13	this	this	DT
cord-283109-ka3n9pft	48	14	mix	mix	NN
cord-283109-ka3n9pft	48	15	was	be	VBD
cord-283109-ka3n9pft	48	16	used	use	VBN
cord-283109-ka3n9pft	48	17	for	for	IN
cord-283109-ka3n9pft	48	18	the	the	DT
cord-283109-ka3n9pft	48	19	RT	RT	NNP
cord-283109-ka3n9pft	48	20	-	-	HYPH
cord-283109-ka3n9pft	48	21	qPCR	qpcr	NN
cord-283109-ka3n9pft	48	22	reaction	reaction	NN
cord-283109-ka3n9pft	48	23	.	.	.
cord-283109-ka3n9pft	49	1	The	the	DT
cord-283109-ka3n9pft	49	2	RT	RT	NNP
cord-283109-ka3n9pft	49	3	-	-	HYPH
cord-283109-ka3n9pft	49	4	qPCR	qpcr	NN
cord-283109-ka3n9pft	49	5	results	result	NNS
cord-283109-ka3n9pft	49	6	showed	show	VBD
cord-283109-ka3n9pft	49	7	that	that	IN
cord-283109-ka3n9pft	49	8	the	the	DT
cord-283109-ka3n9pft	49	9	Ct	ct	JJ
cord-283109-ka3n9pft	49	10	values	value	NNS
cord-283109-ka3n9pft	49	11	of	of	IN
cord-283109-ka3n9pft	49	12	control	control	NN
cord-283109-ka3n9pft	49	13	(	(	-LRB-
cord-283109-ka3n9pft	49	14	without	without	IN
cord-283109-ka3n9pft	49	15	VTM	VTM	NNP
cord-283109-ka3n9pft	49	16	)	)	-RRB-
cord-283109-ka3n9pft	49	17	and	and	CC
cord-283109-ka3n9pft	49	18	VTM	VTM	NNP
cord-283109-ka3n9pft	49	19	mixed	mixed	JJ
cord-283109-ka3n9pft	49	20	reactions	reaction	NNS
cord-283109-ka3n9pft	49	21	(	(	-LRB-
cord-283109-ka3n9pft	49	22	all	all	DT
cord-283109-ka3n9pft	49	23	containing	contain	VBG
cord-283109-ka3n9pft	49	24	400	400	CD
cord-283109-ka3n9pft	49	25	copies	copy	NNS
cord-283109-ka3n9pft	49	26	/	/	SYM
cord-283109-ka3n9pft	49	27	reaction	reaction	NN
cord-283109-ka3n9pft	49	28	)	)	-RRB-
cord-283109-ka3n9pft	49	29	were	be	VBD
cord-283109-ka3n9pft	49	30	very	very	RB
cord-283109-ka3n9pft	49	31	similar	similar	JJ
cord-283109-ka3n9pft	49	32	for	for	IN
cord-283109-ka3n9pft	49	33	all	all	DT
cord-283109-ka3n9pft	49	34	three	three	CD
cord-283109-ka3n9pft	49	35	(	(	-LRB-
cord-283109-ka3n9pft	49	36	N1	N1	NNP
cord-283109-ka3n9pft	49	37	,	,	,
cord-283109-ka3n9pft	49	38	N2	N2	NNP
cord-283109-ka3n9pft	49	39	and	and	CC
cord-283109-ka3n9pft	49	40	N3	n3	NN
cord-283109-ka3n9pft	49	41	)	)	-RRB-
cord-283109-ka3n9pft	50	1	SARS	SARS	NNP
cord-283109-ka3n9pft	50	2	-	-	HYPH
cord-283109-ka3n9pft	50	3	CoV-2	CoV-2	NNP
cord-283109-ka3n9pft	50	4	targets	target	NNS
cord-283109-ka3n9pft	50	5	(	(	-LRB-
cord-283109-ka3n9pft	50	6	Table	table	NN
cord-283109-ka3n9pft	50	7	3	3	CD
cord-283109-ka3n9pft	50	8	)	)	-RRB-
cord-283109-ka3n9pft	50	9	.	.	.
cord-283109-ka3n9pft	51	1	We	-PRON-	PRP
cord-283109-ka3n9pft	51	2	also	also	RB
cord-283109-ka3n9pft	51	3	tested	test	VBD
cord-283109-ka3n9pft	51	4	40	40	CD
cord-283109-ka3n9pft	51	5	copies	copy	NNS
cord-283109-ka3n9pft	51	6	of	of	IN
cord-283109-ka3n9pft	51	7	SARS	SARS	NNP
cord-283109-ka3n9pft	51	8	-	-	HYPH
cord-283109-ka3n9pft	51	9	CoV-2	CoV-2	NNP
cord-283109-ka3n9pft	51	10	plasmid	plasmid	NN
cord-283109-ka3n9pft	51	11	using	use	VBG
cord-283109-ka3n9pft	51	12	RT	RT	NNP
cord-283109-ka3n9pft	51	13	-	-	HYPH
cord-283109-ka3n9pft	51	14	qPCR	qPCR	NNP
cord-283109-ka3n9pft	51	15	,	,	,
cord-283109-ka3n9pft	51	16	which	which	WDT
cord-283109-ka3n9pft	51	17	gave	give	VBD
cord-283109-ka3n9pft	51	18	a	a	DT
cord-283109-ka3n9pft	51	19	Ct	Ct	NNP
cord-283109-ka3n9pft	51	20	value	value	NN
cord-283109-ka3n9pft	51	21	of	of	IN
cord-283109-ka3n9pft	51	22	~38	~38	NFP
cord-283109-ka3n9pft	51	23	(	(	-LRB-
cord-283109-ka3n9pft	51	24	data	datum	NNS
cord-283109-ka3n9pft	51	25	not	not	RB
cord-283109-ka3n9pft	51	26	shown	show	VBN
cord-283109-ka3n9pft	51	27	)	)	-RRB-
cord-283109-ka3n9pft	51	28	.	.	.
cord-283109-ka3n9pft	52	1	This	this	DT
cord-283109-ka3n9pft	52	2	means	mean	VBZ
cord-283109-ka3n9pft	52	3	direct	direct	JJ
cord-283109-ka3n9pft	52	4	spiking	spiking	NN
cord-283109-ka3n9pft	52	5	of	of	IN
cord-283109-ka3n9pft	52	6	a	a	DT
cord-283109-ka3n9pft	52	7	higher	high	JJR
cord-283109-ka3n9pft	52	8	concentration	concentration	NN
cord-283109-ka3n9pft	52	9	of	of	IN
cord-283109-ka3n9pft	52	10	SARS	SARS	NNP
cord-283109-ka3n9pft	52	11	-	-	HYPH
cord-283109-ka3n9pft	52	12	CoV-2	CoV-2	NNP
cord-283109-ka3n9pft	52	13	genome	genome	NN
cord-283109-ka3n9pft	52	14	copies	copy	NNS
cord-283109-ka3n9pft	52	15	in	in	IN
cord-283109-ka3n9pft	52	16	VTM	VTM	NNP
cord-283109-ka3n9pft	52	17	did	do	VBD
cord-283109-ka3n9pft	52	18	not	not	RB
cord-283109-ka3n9pft	52	19	have	have	VB
cord-283109-ka3n9pft	52	20	a	a	DT
cord-283109-ka3n9pft	52	21	major	major	JJ
cord-283109-ka3n9pft	52	22	PCR	PCR	NNP
cord-283109-ka3n9pft	52	23	inhibitory	inhibitory	JJ
cord-283109-ka3n9pft	52	24	effect	effect	NN
cord-283109-ka3n9pft	52	25	in	in	IN
cord-283109-ka3n9pft	52	26	the	the	DT
cord-283109-ka3n9pft	52	27	reactions	reaction	NNS
cord-283109-ka3n9pft	52	28	when	when	WRB
cord-283109-ka3n9pft	52	29	the	the	DT
cord-283109-ka3n9pft	52	30	target	target	NN
cord-283109-ka3n9pft	52	31	concentration	concentration	NN
cord-283109-ka3n9pft	52	32	is	be	VBZ
cord-283109-ka3n9pft	52	33	high	high	JJ
cord-283109-ka3n9pft	53	1	(	(	-LRB-
cord-283109-ka3n9pft	53	2	Table	table	NN
cord-283109-ka3n9pft	53	3	  	  	_SP
cord-283109-ka3n9pft	53	4	3	3	CD
cord-283109-ka3n9pft	53	5	)	)	-RRB-
cord-283109-ka3n9pft	53	6	.	.	.
cord-283109-ka3n9pft	54	1	Therefore	therefore	RB
cord-283109-ka3n9pft	54	2	,	,	,
cord-283109-ka3n9pft	54	3	it	-PRON-	PRP
cord-283109-ka3n9pft	54	4	is	be	VBZ
cord-283109-ka3n9pft	54	5	likely	likely	JJ
cord-283109-ka3n9pft	54	6	that	that	IN
cord-283109-ka3n9pft	54	7	specimens	specimen	NNS
cord-283109-ka3n9pft	54	8	with	with	IN
cord-283109-ka3n9pft	54	9	higher	high	JJR
cord-283109-ka3n9pft	54	10	viral	viral	JJ
cord-283109-ka3n9pft	54	11	load	load	NN
cord-283109-ka3n9pft	54	12	could	could	MD
cord-283109-ka3n9pft	54	13	also	also	RB
cord-283109-ka3n9pft	54	14	be	be	VB
cord-283109-ka3n9pft	54	15	detected	detect	VBN
cord-283109-ka3n9pft	54	16	by	by	IN
cord-283109-ka3n9pft	54	17	RT	RT	NNP
cord-283109-ka3n9pft	54	18	-	-	HYPH
cord-283109-ka3n9pft	54	19	qPCR	qPCR	NNP
cord-283109-ka3n9pft	54	20	without	without	IN
cord-283109-ka3n9pft	54	21	needing	need	VBG
cord-283109-ka3n9pft	54	22	an	an	DT
cord-283109-ka3n9pft	54	23	RNA	RNA	NNP
cord-283109-ka3n9pft	54	24	extraction	extraction	NN
cord-283109-ka3n9pft	54	25	step	step	NN
cord-283109-ka3n9pft	54	26	.	.	.
cord-283109-ka3n9pft	55	1	SARS	SARS	NNP
cord-283109-ka3n9pft	55	2	-	-	HYPH
cord-283109-ka3n9pft	55	3	CoV-2	CoV-2	NNP
cord-283109-ka3n9pft	55	4	plasmid	plasmid	NN
cord-283109-ka3n9pft	55	5	in	in	IN
cord-283109-ka3n9pft	55	6	VTM	VTM	NNP
cord-283109-ka3n9pft	55	7	did	do	VBD
cord-283109-ka3n9pft	55	8	not	not	RB
cord-283109-ka3n9pft	55	9	show	show	VB
cord-283109-ka3n9pft	55	10	any	any	DT
cord-283109-ka3n9pft	55	11	PCR	PCR	NNP
cord-283109-ka3n9pft	55	12	inhibition	inhibition	NN
cord-283109-ka3n9pft	55	13	when	when	WRB
cord-283109-ka3n9pft	55	14	compared	compare	VBN
cord-283109-ka3n9pft	55	15	to	to	IN
cord-283109-ka3n9pft	55	16	plasmid	plasmid	NN
cord-283109-ka3n9pft	55	17	alone	alone	RB
cord-283109-ka3n9pft	55	18	.	.	.
cord-283109-ka3n9pft	56	1	The	the	DT
cord-283109-ka3n9pft	56	2	CDC	CDC	NNP
cord-283109-ka3n9pft	56	3	positive	positive	JJ
cord-283109-ka3n9pft	56	4	control	control	NN
cord-283109-ka3n9pft	56	5	plasmid	plasmid	NN
cord-283109-ka3n9pft	56	6	for	for	IN
cord-283109-ka3n9pft	56	7	SARS	SARS	NNP
cord-283109-ka3n9pft	56	8	-	-	HYPH
cord-283109-ka3n9pft	56	9	CoV-2	CoV-2	NNP
cord-283109-ka3n9pft	56	10	(	(	-LRB-
cord-283109-ka3n9pft	56	11	from	from	IN
cord-283109-ka3n9pft	56	12	IDT	IDT	NNP
cord-283109-ka3n9pft	56	13	)	)	-RRB-
cord-283109-ka3n9pft	56	14	was	be	VBD
cord-283109-ka3n9pft	56	15	diluted	dilute	VBN
cord-283109-ka3n9pft	56	16	in	in	IN
cord-283109-ka3n9pft	56	17	VTM	VTM	NNP
cord-283109-ka3n9pft	56	18	or	or	CC
cord-283109-ka3n9pft	56	19	TE	TE	NNP
cord-283109-ka3n9pft	56	20	buffer	buffer	NN
cord-283109-ka3n9pft	56	21	(	(	-LRB-
cord-283109-ka3n9pft	56	22	control	control	NN
cord-283109-ka3n9pft	56	23	)	)	-RRB-
cord-283109-ka3n9pft	56	24	to	to	TO
cord-283109-ka3n9pft	56	25	get	get	VB
cord-283109-ka3n9pft	56	26	100,000	100,000	CD
cord-283109-ka3n9pft	56	27	copies	copy	NNS
cord-283109-ka3n9pft	56	28	per	per	IN
cord-283109-ka3n9pft	56	29	mL.	ml.	NN
cord-283109-ka3n9pft	56	30	4	4	CD
cord-283109-ka3n9pft	56	31	µL	µl	CD
cord-283109-ka3n9pft	56	32	of	of	IN
cord-283109-ka3n9pft	56	33	SARS	SARS	NNP
cord-283109-ka3n9pft	56	34	-	-	HYPH
cord-283109-ka3n9pft	56	35	CoV-2	CoV-2	NNP
cord-283109-ka3n9pft	56	36	plasmids	plasmid	NNS
cord-283109-ka3n9pft	56	37	in	in	IN
cord-283109-ka3n9pft	56	38	VTM	VTM	NNP
cord-283109-ka3n9pft	56	39	and	and	CC
cord-283109-ka3n9pft	56	40	in	in	IN
cord-283109-ka3n9pft	56	41	TE	TE	NNP
cord-283109-ka3n9pft	56	42	buffer	buffer	NN
cord-283109-ka3n9pft	56	43	were	be	VBD
cord-283109-ka3n9pft	56	44	added	add	VBN
cord-283109-ka3n9pft	56	45	to	to	IN
cord-283109-ka3n9pft	56	46	a	a	DT
cord-283109-ka3n9pft	56	47	20	20	CD
cord-283109-ka3n9pft	56	48	µL	µl	NN
cord-283109-ka3n9pft	56	49	PCR	PCR	NNP
cord-283109-ka3n9pft	56	50	reaction	reaction	NN
cord-283109-ka3n9pft	56	51	mix	mix	NN
cord-283109-ka3n9pft	56	52	targeting	target	VBG
cord-283109-ka3n9pft	56	53	N1	N1	NNP
cord-283109-ka3n9pft	56	54	,	,	,
cord-283109-ka3n9pft	56	55	N2	N2	NNP
cord-283109-ka3n9pft	56	56	and	and	CC
cord-283109-ka3n9pft	56	57	N3	n3	NN
cord-283109-ka3n9pft	56	58	genes	gene	NNS
cord-283109-ka3n9pft	56	59	for	for	IN
cord-283109-ka3n9pft	56	60	the	the	DT
cord-283109-ka3n9pft	56	61	detection	detection	NN
cord-283109-ka3n9pft	56	62	of	of	IN
cord-283109-ka3n9pft	56	63	SARS	SARS	NNP
cord-283109-ka3n9pft	56	64	-	-	HYPH
cord-283109-ka3n9pft	56	65	CoV-2	CoV-2	NNP
cord-283109-ka3n9pft	56	66	.	.	.
cord-283109-ka3n9pft	57	1	The	the	DT
cord-283109-ka3n9pft	57	2	Ct	ct	JJ
cord-283109-ka3n9pft	57	3	values	value	NNS
cord-283109-ka3n9pft	57	4	of	of	IN
cord-283109-ka3n9pft	57	5	both	both	CC
cord-283109-ka3n9pft	57	6	the	the	DT
cord-283109-ka3n9pft	57	7	control	control	NN
cord-283109-ka3n9pft	57	8	and	and	CC
cord-283109-ka3n9pft	57	9	plasmid	plasmid	NN
cord-283109-ka3n9pft	57	10	in	in	IN
cord-283109-ka3n9pft	57	11	VTM	VTM	NNP
cord-283109-ka3n9pft	57	12	are	be	VBP
cord-283109-ka3n9pft	57	13	very	very	RB
cord-283109-ka3n9pft	57	14	similar	similar	JJ
cord-283109-ka3n9pft	57	15	,	,	,
cord-283109-ka3n9pft	57	16	indicating	indicate	VBG
cord-283109-ka3n9pft	57	17	that	that	IN
cord-283109-ka3n9pft	57	18	the	the	DT
cord-283109-ka3n9pft	57	19	sample	sample	NN
cord-283109-ka3n9pft	57	20	preparation	preparation	NN
cord-283109-ka3n9pft	57	21	step	step	NN
cord-283109-ka3n9pft	57	22	can	can	MD
cord-283109-ka3n9pft	57	23	be	be	VB
cord-283109-ka3n9pft	57	24	omitted	omit	VBN
cord-283109-ka3n9pft	57	25	in	in	IN
cord-283109-ka3n9pft	57	26	high	high	JJ
cord-283109-ka3n9pft	57	27	viral	viral	JJ
cord-283109-ka3n9pft	57	28	load	load	NN
cord-283109-ka3n9pft	57	29	samples	sample	NNS
cord-283109-ka3n9pft	57	30	.	.	.
cord-283109-ka3n9pft	58	1	Our	-PRON-	PRP$
cord-283109-ka3n9pft	58	2	data	datum	NNS
cord-283109-ka3n9pft	58	3	using	use	VBG
cord-283109-ka3n9pft	58	4	both	both	CC
cord-283109-ka3n9pft	58	5	high	high	JJ
cord-283109-ka3n9pft	58	6	(	(	-LRB-
cord-283109-ka3n9pft	58	7	Ct	Ct	NNP
cord-283109-ka3n9pft	58	8	<	<	XX
cord-283109-ka3n9pft	58	9	35	35	CD
cord-283109-ka3n9pft	58	10	)	)	-RRB-
cord-283109-ka3n9pft	58	11	and	and	CC
cord-283109-ka3n9pft	58	12	low	low	JJ
cord-283109-ka3n9pft	58	13	(	(	-LRB-
cord-283109-ka3n9pft	58	14	Ct	Ct	NNP
cord-283109-ka3n9pft	58	15	>	>	XX
cord-283109-ka3n9pft	58	16	35	35	CD
cord-283109-ka3n9pft	58	17	)	)	-RRB-
cord-283109-ka3n9pft	58	18	target	target	NN
cord-283109-ka3n9pft	58	19	concentrations	concentration	NNS
cord-283109-ka3n9pft	58	20	suggest	suggest	VBP
cord-283109-ka3n9pft	58	21	that	that	IN
cord-283109-ka3n9pft	58	22	since	since	IN
cord-283109-ka3n9pft	58	23	RT	RT	NNP
cord-283109-ka3n9pft	58	24	-	-	HYPH
cord-283109-ka3n9pft	58	25	qPCR	qPCR	NNP
cord-283109-ka3n9pft	58	26	is	be	VBZ
cord-283109-ka3n9pft	58	27	highly	highly	RB
cord-283109-ka3n9pft	58	28	sensitive	sensitive	JJ
cord-283109-ka3n9pft	58	29	,	,	,
cord-283109-ka3n9pft	58	30	using	use	VBG
cord-283109-ka3n9pft	58	31	raw	raw	JJ
cord-283109-ka3n9pft	58	32	samples	sample	NNS
cord-283109-ka3n9pft	58	33	or	or	CC
cord-283109-ka3n9pft	58	34	minimal	minimal	JJ
cord-283109-ka3n9pft	58	35	sample	sample	NN
cord-283109-ka3n9pft	58	36	preparation	preparation	NN
cord-283109-ka3n9pft	58	37	steps	step	NNS
cord-283109-ka3n9pft	58	38	might	may	MD
cord-283109-ka3n9pft	58	39	not	not	RB
cord-283109-ka3n9pft	58	40	reduce	reduce	VB
cord-283109-ka3n9pft	58	41	the	the	DT
cord-283109-ka3n9pft	58	42	test	test	NN
cord-283109-ka3n9pft	58	43	sensitivity	sensitivity	NN
cord-283109-ka3n9pft	58	44	as	as	IN
cord-283109-ka3n9pft	58	45	most	most	JJS
cord-283109-ka3n9pft	58	46	patients	patient	NNS
cord-283109-ka3n9pft	58	47	tend	tend	VBP
cord-283109-ka3n9pft	58	48	to	to	TO
cord-283109-ka3n9pft	58	49	have	have	VB
cord-283109-ka3n9pft	58	50	a	a	DT
cord-283109-ka3n9pft	58	51	higher	high	JJR
cord-283109-ka3n9pft	58	52	viral	viral	JJ
cord-283109-ka3n9pft	58	53	load	load	NN
cord-283109-ka3n9pft	58	54	.	.	.
cord-283109-ka3n9pft	59	1	5	5	LS
cord-283109-ka3n9pft	60	1	Also	also	RB
cord-283109-ka3n9pft	60	2	,	,	,
cord-283109-ka3n9pft	60	3	the	the	DT
cord-283109-ka3n9pft	60	4	efficiency	efficiency	NN
cord-283109-ka3n9pft	60	5	of	of	IN
cord-283109-ka3n9pft	60	6	extraction	extraction	NN
cord-283109-ka3n9pft	60	7	methods	method	NNS
cord-283109-ka3n9pft	60	8	tends	tend	VBZ
cord-283109-ka3n9pft	60	9	to	to	TO
cord-283109-ka3n9pft	60	10	drop	drop	VB
cord-283109-ka3n9pft	60	11	significantly	significantly	RB
cord-283109-ka3n9pft	60	12	at	at	IN
cord-283109-ka3n9pft	60	13	very	very	RB
cord-283109-ka3n9pft	60	14	low	low	JJ
cord-283109-ka3n9pft	60	15	target	target	NN
cord-283109-ka3n9pft	60	16	concentrations	concentration	NNS
cord-283109-ka3n9pft	60	17	when	when	WRB
cord-283109-ka3n9pft	60	18	processed	process	VBN
cord-283109-ka3n9pft	60	19	through	through	IN
cord-283109-ka3n9pft	60	20	numerous	numerous	JJ
cord-283109-ka3n9pft	60	21	washing	washing	NN
cord-283109-ka3n9pft	60	22	steps	step	NNS
cord-283109-ka3n9pft	60	23	.	.	.
cord-283109-ka3n9pft	61	1	Therefore	therefore	RB
cord-283109-ka3n9pft	61	2	,	,	,
cord-283109-ka3n9pft	61	3	nucleic	nucleic	JJ
cord-283109-ka3n9pft	61	4	acid	acid	NN
cord-283109-ka3n9pft	61	5	loss	loss	NN
cord-283109-ka3n9pft	61	6	during	during	IN
cord-283109-ka3n9pft	61	7	extraction	extraction	NN
cord-283109-ka3n9pft	61	8	steps	step	NNS
cord-283109-ka3n9pft	61	9	may	may	MD
cord-283109-ka3n9pft	61	10	hurt	hurt	VB
cord-283109-ka3n9pft	61	11	the	the	DT
cord-283109-ka3n9pft	61	12	limit	limit	NN
cord-283109-ka3n9pft	61	13	of	of	IN
cord-283109-ka3n9pft	61	14	detection	detection	NN
cord-283109-ka3n9pft	61	15	.	.	.
cord-283109-ka3n9pft	62	1	While	while	IN
cord-283109-ka3n9pft	62	2	further	further	JJ
cord-283109-ka3n9pft	62	3	studies	study	NNS
cord-283109-ka3n9pft	62	4	with	with	IN
cord-283109-ka3n9pft	62	5	patient	patient	JJ
cord-283109-ka3n9pft	62	6	specimens	specimen	NNS
cord-283109-ka3n9pft	62	7	and	and	CC
cord-283109-ka3n9pft	62	8	a	a	DT
cord-283109-ka3n9pft	62	9	higher	high	JJR
cord-283109-ka3n9pft	62	10	number	number	NN
cord-283109-ka3n9pft	62	11	of	of	IN
cord-283109-ka3n9pft	62	12	samples	sample	NNS
cord-283109-ka3n9pft	62	13	are	be	VBP
cord-283109-ka3n9pft	62	14	needed	need	VBN
cord-283109-ka3n9pft	62	15	to	to	TO
cord-283109-ka3n9pft	62	16	confirm	confirm	VB
cord-283109-ka3n9pft	62	17	our	-PRON-	PRP$
cord-283109-ka3n9pft	62	18	preliminary	preliminary	JJ
cord-283109-ka3n9pft	62	19	results	result	NNS
cord-283109-ka3n9pft	62	20	,	,	,
cord-283109-ka3n9pft	62	21	we	-PRON-	PRP
cord-283109-ka3n9pft	62	22	report	report	VBP
cord-283109-ka3n9pft	62	23	that	that	IN
cord-283109-ka3n9pft	62	24	the	the	DT
cord-283109-ka3n9pft	62	25	use	use	NN
cord-283109-ka3n9pft	62	26	of	of	IN
cord-283109-ka3n9pft	62	27	untreated	untreated	JJ
cord-283109-ka3n9pft	62	28	samples	sample	NNS
cord-283109-ka3n9pft	62	29	can	can	MD
cord-283109-ka3n9pft	62	30	be	be	VB
cord-283109-ka3n9pft	62	31	a	a	DT
cord-283109-ka3n9pft	62	32	viable	viable	JJ
cord-283109-ka3n9pft	62	33	option	option	NN
cord-283109-ka3n9pft	62	34	during	during	IN
cord-283109-ka3n9pft	62	35	the	the	DT
cord-283109-ka3n9pft	62	36	COVID-19	COVID-19	NNP
cord-283109-ka3n9pft	62	37	pandemic	pandemic	NN
cord-283109-ka3n9pft	62	38	.	.	.
cord-283109-ka3n9pft	63	1	The	the	DT
cord-283109-ka3n9pft	63	2	 	 	_SP
cord-283109-ka3n9pft	63	3	AccuPlex	AccuPlex	NNP
cord-283109-ka3n9pft	63	4	SARS	SARS	NNP
cord-283109-ka3n9pft	63	5	-	-	HYPH
cord-283109-ka3n9pft	63	6	CoV-2	CoV-2	NNP
cord-283109-ka3n9pft	63	7	reference	reference	NN
cord-283109-ka3n9pft	63	8	material	material	NN
cord-283109-ka3n9pft	63	9	from	from	IN
cord-283109-ka3n9pft	63	10	SeraCare	SeraCare	NNP
cord-283109-ka3n9pft	63	11	containing	contain	VBG
cord-283109-ka3n9pft	63	12	con	con	JJ
cord-283109-ka3n9pft	63	13	-	-	HYPH
cord-283109-ka3n9pft	63	14	replicative	replicative	JJ
cord-283109-ka3n9pft	63	15	viral	viral	JJ
cord-283109-ka3n9pft	63	16	particles	particle	NNS
cord-283109-ka3n9pft	63	17	(	(	-LRB-
cord-283109-ka3n9pft	63	18	5,000	5,000	CD
cord-283109-ka3n9pft	63	19	viral	viral	JJ
cord-283109-ka3n9pft	63	20	particles	particle	NNS
cord-283109-ka3n9pft	63	21	per	per	IN
cord-283109-ka3n9pft	63	22	mL	ml	NN
cord-283109-ka3n9pft	63	23	)	)	-RRB-
cord-283109-ka3n9pft	63	24	was	be	VBD
cord-283109-ka3n9pft	63	25	mixed	mix	VBN
cord-283109-ka3n9pft	63	26	with	with	IN
cord-283109-ka3n9pft	63	27	equal	equal	JJ
cord-283109-ka3n9pft	63	28	volumes	volume	NNS
cord-283109-ka3n9pft	63	29	of	of	IN
cord-283109-ka3n9pft	63	30	VTM	VTM	NNP
cord-283109-ka3n9pft	63	31	to	to	TO
cord-283109-ka3n9pft	63	32	get	get	VB
cord-283109-ka3n9pft	63	33	a	a	DT
cord-283109-ka3n9pft	63	34	final	final	JJ
cord-283109-ka3n9pft	63	35	concentration	concentration	NN
cord-283109-ka3n9pft	63	36	of	of	IN
cord-283109-ka3n9pft	63	37	2,500	2,500	CD
cord-283109-ka3n9pft	63	38	viral	viral	JJ
cord-283109-ka3n9pft	63	39	particles	particle	NNS
cord-283109-ka3n9pft	63	40	per	per	IN
cord-283109-ka3n9pft	63	41	mL.	mL.	NNS
cord-283109-ka3n9pft	63	42	Each	each	DT
cord-283109-ka3n9pft	63	43	microliter	microliter	NN
cord-283109-ka3n9pft	63	44	contained	contain	VBD
cord-283109-ka3n9pft	63	45	~2.5	~2.5	CD
cord-283109-ka3n9pft	63	46	genomic	genomic	JJ
cord-283109-ka3n9pft	63	47	material	material	NN
cord-283109-ka3n9pft	63	48	equivalents	equivalent	NNS
cord-283109-ka3n9pft	63	49	of	of	IN
cord-283109-ka3n9pft	63	50	SARS	SARS	NNP
cord-283109-ka3n9pft	63	51	-	-	HYPH
cord-283109-ka3n9pft	63	52	CoV-2	CoV-2	NNP
cord-283109-ka3n9pft	63	53	.	.	.
cord-283109-ka3n9pft	64	1	SARS	SARS	NNP
cord-283109-ka3n9pft	64	2	-	-	HYPH
cord-283109-ka3n9pft	64	3	CoV-2	CoV-2	NNP
cord-283109-ka3n9pft	64	4	positive	positive	JJ
cord-283109-ka3n9pft	64	5	control	control	NN
cord-283109-ka3n9pft	64	6	plasmid	plasmid	NN
cord-283109-ka3n9pft	64	7	(	(	-LRB-
cord-283109-ka3n9pft	64	8	CDC	CDC	NNP
cord-283109-ka3n9pft	64	9	recommended	recommend	VBD
cord-283109-ka3n9pft	64	10	)	)	-RRB-
cord-283109-ka3n9pft	64	11	was	be	VBD
cord-283109-ka3n9pft	64	12	obtained	obtain	VBN
cord-283109-ka3n9pft	64	13	from	from	IN
cord-283109-ka3n9pft	64	14	Integrated	Integrated	NNP
cord-283109-ka3n9pft	64	15	DNA	DNA	NNP
cord-283109-ka3n9pft	64	16	Technologies	Technologies	NNP
cord-283109-ka3n9pft	64	17	(	(	-LRB-
cord-283109-ka3n9pft	64	18	200,000	200,000	CD
cord-283109-ka3n9pft	64	19	copies/µL	copies/µL	NNS
cord-283109-ka3n9pft	64	20	)	)	-RRB-
cord-283109-ka3n9pft	64	21	.	.	.
cord-283109-ka3n9pft	65	1	This	this	DT
cord-283109-ka3n9pft	65	2	was	be	VBD
cord-283109-ka3n9pft	65	3	diluted	dilute	VBN
cord-283109-ka3n9pft	65	4	to	to	IN
cord-283109-ka3n9pft	65	5	10,000	10,000	CD
cord-283109-ka3n9pft	65	6	copies/µL	copies/µL	NNS
cord-283109-ka3n9pft	65	7	in	in	IN
cord-283109-ka3n9pft	65	8	TE	TE	NNP
cord-283109-ka3n9pft	65	9	buffer	buffer	NN
cord-283109-ka3n9pft	65	10	and	and	CC
cord-283109-ka3n9pft	65	11	copies/	copies/	NN
cord-283109-ka3n9pft	66	1	mL.	mL.	NNP
cord-283109-ka3n9pft	66	2	A	a	DT
cord-283109-ka3n9pft	66	3	control	control	NN
cord-283109-ka3n9pft	66	4	was	be	VBD
cord-283109-ka3n9pft	66	5	prepared	prepare	VBN
cord-283109-ka3n9pft	66	6	by	by	IN
cord-283109-ka3n9pft	66	7	diluting	dilute	VBG
cord-283109-ka3n9pft	66	8	10	10	CD
cord-283109-ka3n9pft	66	9	µL	µl	CD
cord-283109-ka3n9pft	66	10	of	of	IN
cord-283109-ka3n9pft	66	11	plasmid	plasmid	NN
cord-283109-ka3n9pft	66	12	stock	stock	NN
cord-283109-ka3n9pft	66	13	solution	solution	NN
cord-283109-ka3n9pft	66	14	in	in	IN
cord-283109-ka3n9pft	66	15	990	990	CD
cord-283109-ka3n9pft	66	16	µL	µl	CD
cord-283109-ka3n9pft	66	17	of	of	IN
cord-283109-ka3n9pft	66	18	TE	TE	NNP
cord-283109-ka3n9pft	66	19	buffer	buffer	NN
cord-283109-ka3n9pft	66	20	.	.	.
cord-283109-ka3n9pft	67	1	Both	both	DT
cord-283109-ka3n9pft	67	2	the	the	DT
cord-283109-ka3n9pft	67	3	VTM	VTM	NNP
cord-283109-ka3n9pft	67	4	and	and	CC
cord-283109-ka3n9pft	67	5	TE	TE	NNP
cord-283109-ka3n9pft	67	6	buffer	buffer	NN
cord-283109-ka3n9pft	67	7	working	working	NN
cord-283109-ka3n9pft	67	8	solutions	solution	NNS
cord-283109-ka3n9pft	67	9	(	(	-LRB-
cord-283109-ka3n9pft	67	10	4	4	CD
cord-283109-ka3n9pft	67	11	µL	µl	CD
cord-283109-ka3n9pft	67	12	or	or	CC
cord-283109-ka3n9pft	67	13	400	400	CD
cord-283109-ka3n9pft	67	14	copies	copy	NNS
cord-283109-ka3n9pft	67	15	in	in	IN
cord-283109-ka3n9pft	67	16	each	each	DT
cord-283109-ka3n9pft	67	17	reaction	reaction	NN
cord-283109-ka3n9pft	67	18	)	)	-RRB-
cord-283109-ka3n9pft	67	19	were	be	VBD
cord-283109-ka3n9pft	67	20	added	add	VBN
cord-283109-ka3n9pft	67	21	into	into	IN
cord-283109-ka3n9pft	67	22	a	a	DT
cord-283109-ka3n9pft	67	23	20	20	CD
cord-283109-ka3n9pft	67	24	µL	µl	NN
cord-283109-ka3n9pft	67	25	PCR	PCR	NNP
cord-283109-ka3n9pft	67	26	reaction	reaction	NN
cord-283109-ka3n9pft	67	27	.	.	.
cord-283109-ka3n9pft	68	1	The	the	DT
cord-283109-ka3n9pft	68	2	Promega	Promega	NNP
cord-283109-ka3n9pft	68	3	Maxwell	Maxwell	NNP
cord-283109-ka3n9pft	68	4	extraction	extraction	NN
cord-283109-ka3n9pft	68	5	was	be	VBD
cord-283109-ka3n9pft	68	6	performed	perform	VBN
cord-283109-ka3n9pft	68	7	using	use	VBG
cord-283109-ka3n9pft	68	8	AS1520	AS1520	NNP
cord-283109-ka3n9pft	68	9	cartridge	cartridge	NN
cord-283109-ka3n9pft	68	10	(	(	-LRB-
cord-283109-ka3n9pft	68	11	Promega	Promega	NNP
cord-283109-ka3n9pft	68	12	Corporation	Corporation	NNP
cord-283109-ka3n9pft	68	13	,	,	,
cord-283109-ka3n9pft	68	14	Madison	Madison	NNP
cord-283109-ka3n9pft	68	15	,	,	,
cord-283109-ka3n9pft	68	16	WI	WI	NNP
cord-283109-ka3n9pft	68	17	)	)	-RRB-
cord-283109-ka3n9pft	68	18	.	.	.
cord-283109-ka3n9pft	69	1	100	100	CD
cord-283109-ka3n9pft	69	2	µL	µl	CD
cord-283109-ka3n9pft	69	3	of	of	IN
cord-283109-ka3n9pft	69	4	the	the	DT
cord-283109-ka3n9pft	69	5	sample	sample	NN
cord-283109-ka3n9pft	69	6	(	(	-LRB-
cord-283109-ka3n9pft	70	1	AccuPlex	AccuPlex	NNP
cord-283109-ka3n9pft	70	2	SARS	SARS	NNP
cord-283109-ka3n9pft	70	3	-	-	HYPH
cord-283109-ka3n9pft	70	4	CoV-2	CoV-2	NNP
cord-283109-ka3n9pft	70	5	or	or	CC
cord-283109-ka3n9pft	70	6	Influenza	Influenza	NNP
cord-283109-ka3n9pft	70	7	A	A	NNP
cord-283109-ka3n9pft	70	8	/	/	SYM
cord-283109-ka3n9pft	70	9	B	b	NN
cord-283109-ka3n9pft	70	10	or	or	CC
cord-283109-ka3n9pft	70	11	RSV	RSV	NNP
cord-283109-ka3n9pft	70	12	)	)	-RRB-
cord-283109-ka3n9pft	70	13	was	be	VBD
cord-283109-ka3n9pft	70	14	added	add	VBN
cord-283109-ka3n9pft	70	15	to	to	IN
cord-283109-ka3n9pft	70	16	the	the	DT
cord-283109-ka3n9pft	70	17	cartridge	cartridge	NN
cord-283109-ka3n9pft	70	18	and	and	CC
cord-283109-ka3n9pft	70	19	eluted	elute	VBD
cord-283109-ka3n9pft	70	20	in	in	IN
cord-283109-ka3n9pft	70	21	100	100	CD
cord-283109-ka3n9pft	70	22	µL	µl	IN
cord-283109-ka3n9pft	70	23	elution	elution	NN
cord-283109-ka3n9pft	70	24	buffer	buffer	NN
cord-283109-ka3n9pft	70	25	provided	provide	VBN
cord-283109-ka3n9pft	70	26	in	in	IN
cord-283109-ka3n9pft	70	27	the	the	DT
cord-283109-ka3n9pft	70	28	kit	kit	NN
cord-283109-ka3n9pft	70	29	.	.	.
cord-283109-ka3n9pft	70	30	 	 	_SP
cord-283109-ka3n9pft	71	1	The	the	DT
cord-283109-ka3n9pft	71	2	authors	author	NNS
cord-283109-ka3n9pft	71	3	declare	declare	VBP
cord-283109-ka3n9pft	71	4	no	no	DT
cord-283109-ka3n9pft	71	5	conflict	conflict	NN
cord-283109-ka3n9pft	71	6	of	of	IN
cord-283109-ka3n9pft	71	7	interest	interest	NN
cord-283109-ka3n9pft	71	8	.	.	.
cord-283109-ka3n9pft	71	9	 	 	_SP
cord-283109-ka3n9pft	72	1	Molecular	molecular	JJ
cord-283109-ka3n9pft	72	2	Diagnosis	Diagnosis	NNP
cord-283109-ka3n9pft	72	3	of	of	IN
cord-283109-ka3n9pft	72	4	a	a	DT
cord-283109-ka3n9pft	72	5	Novel	Novel	NNP
cord-283109-ka3n9pft	72	6	Coronavirus	Coronavirus	NNP
cord-283109-ka3n9pft	72	7	(	(	-LRB-
cord-283109-ka3n9pft	72	8	2019-nCoV	2019-ncov	CD
cord-283109-ka3n9pft	72	9	)	)	-RRB-
cord-283109-ka3n9pft	73	1	Causing	cause	VBG
cord-283109-ka3n9pft	73	2	an	an	DT
cord-283109-ka3n9pft	73	3	Outbreak	outbreak	NN
cord-283109-ka3n9pft	73	4	of	of	IN
cord-283109-ka3n9pft	73	5	Pneumonia	Pneumonia	NNP
cord-283109-ka3n9pft	73	6	A	A	NNP
cord-283109-ka3n9pft	73	7	Novel	Novel	NNP
cord-283109-ka3n9pft	73	8	Coronavirus	Coronavirus	NNP
cord-283109-ka3n9pft	73	9	from	from	IN
cord-283109-ka3n9pft	73	10	Patients	patient	NNS
cord-283109-ka3n9pft	73	11	with	with	IN
cord-283109-ka3n9pft	73	12	Pneumonia	Pneumonia	NNP
cord-283109-ka3n9pft	73	13	in	in	IN
cord-283109-ka3n9pft	73	14	China	China	NNP
cord-283109-ka3n9pft	74	1	Comparison	comparison	NN
cord-283109-ka3n9pft	74	2	of	of	IN
cord-283109-ka3n9pft	74	3	different	different	JJ
cord-283109-ka3n9pft	74	4	samples	sample	NNS
cord-283109-ka3n9pft	74	5	for	for	IN
cord-283109-ka3n9pft	74	6	2019	2019	CD
cord-283109-ka3n9pft	74	7	novel	novel	JJ
cord-283109-ka3n9pft	74	8	coronavirus	coronavirus	NN
cord-283109-ka3n9pft	74	9	detection	detection	NN
cord-283109-ka3n9pft	74	10	by	by	IN
cord-283109-ka3n9pft	74	11	nucleic	nucleic	JJ
cord-283109-ka3n9pft	74	12	acid	acid	NN
cord-283109-ka3n9pft	74	13	amplification	amplification	NN
cord-283109-ka3n9pft	74	14	tests	test	VBZ
cord-283109-ka3n9pft	74	15	Detection	detection	NN
cord-283109-ka3n9pft	74	16	of	of	IN
cord-283109-ka3n9pft	74	17	2019	2019	CD
cord-283109-ka3n9pft	74	18	novel	novel	JJ
cord-283109-ka3n9pft	74	19	coronavirus	coronavirus	NN
cord-283109-ka3n9pft	74	20	(	(	-LRB-
cord-283109-ka3n9pft	74	21	2019-nCoV	2019-ncov	CD
cord-283109-ka3n9pft	74	22	)	)	-RRB-
cord-283109-ka3n9pft	74	23	by	by	IN
cord-283109-ka3n9pft	74	24	real	real	JJ
cord-283109-ka3n9pft	74	25	-	-	HYPH
cord-283109-ka3n9pft	74	26	time	time	NN
cord-283109-ka3n9pft	74	27	RT	RT	NNP
cord-283109-ka3n9pft	74	28	-	-	HYPH
cord-283109-ka3n9pft	74	29	PCR	PCR	NNP
cord-283109-ka3n9pft	74	30	SARS	SARS	NNP
cord-283109-ka3n9pft	74	31	-	-	HYPH
cord-283109-ka3n9pft	74	32	CoV-2	CoV-2	NNP
cord-283109-ka3n9pft	74	33	Viral	Viral	NNP
cord-283109-ka3n9pft	74	34	Load	Load	NNP
cord-283109-ka3n9pft	74	35	in	in	IN
cord-283109-ka3n9pft	74	36	Upper	Upper	NNP
cord-283109-ka3n9pft	74	37	Respiratory	respiratory	JJ
cord-283109-ka3n9pft	74	38	Specimens	specimen	NNS
cord-283109-ka3n9pft	74	39	of	of	IN
cord-283109-ka3n9pft	74	40	Infected	Infected	NNP
cord-283109-ka3n9pft	74	41	Patients	Patients	NNPS
cord-283109-ka3n9pft	74	42	Evaluation	Evaluation	NNP
cord-283109-ka3n9pft	74	43	of	of	IN
cord-283109-ka3n9pft	74	44	Three	three	CD
cord-283109-ka3n9pft	74	45	Influenza	Influenza	NNP
cord-283109-ka3n9pft	74	46	A	A	NNP
cord-283109-ka3n9pft	74	47	and	and	CC
cord-283109-ka3n9pft	74	48	B	B	NNP
cord-283109-ka3n9pft	74	49	Real	real	JJ
cord-283109-ka3n9pft	74	50	-	-	HYPH
cord-283109-ka3n9pft	74	51	Time	Time	NNP
cord-283109-ka3n9pft	74	52	Reverse	Reverse	NNP
cord-283109-ka3n9pft	74	53	Transcription	Transcription	NNP
cord-283109-ka3n9pft	74	54	-	-	HYPH
cord-283109-ka3n9pft	74	55	PCR	PCR	NNP
cord-283109-ka3n9pft	74	56	Assays	Assays	NNPS
cord-283109-ka3n9pft	74	57	and	and	CC
cord-283109-ka3n9pft	74	58	a	a	DT
cord-283109-ka3n9pft	74	59	New	New	NNP
cord-283109-ka3n9pft	74	60	2009	2009	CD
cord-283109-ka3n9pft	74	61	H1N1	H1N1	NNP
cord-283109-ka3n9pft	74	62	Assay	Assay	NNP
cord-283109-ka3n9pft	74	63	for	for	IN
cord-283109-ka3n9pft	74	64	Detection	Detection	NNP
cord-283109-ka3n9pft	74	65	of	of	IN
cord-283109-ka3n9pft	74	66	Influenza	Influenza	NNP
cord-283109-ka3n9pft	74	67	Viruses	virus	NNS
cord-283109-ka3n9pft	75	1	The	the	DT
cord-283109-ka3n9pft	75	2	Burden	Burden	NNP
cord-283109-ka3n9pft	75	3	of	of	IN
cord-283109-ka3n9pft	75	4	Hospitalized	Hospitalized	NNP
cord-283109-ka3n9pft	75	5	Lower	Lower	NNP
cord-283109-ka3n9pft	75	6	Respiratory	respiratory	JJ
cord-283109-ka3n9pft	75	7	Tract	tract	NN
cord-283109-ka3n9pft	75	8	Infection	infection	NN
cord-283109-ka3n9pft	75	9	due	due	JJ
cord-283109-ka3n9pft	75	10	to	to	IN
cord-283109-ka3n9pft	75	11	Respiratory	respiratory	JJ
cord-283109-ka3n9pft	75	12	Syncytial	Syncytial	NNP
cord-283109-ka3n9pft	75	13	Virus	virus	NN
cord-283109-ka3n9pft	75	14	in	in	IN
cord-283109-ka3n9pft	75	15	Rural	Rural	NNP
cord-283109-ka3n9pft	75	16	Thailand	Thailand	NNP
cord-283109-ka3n9pft	75	17	Division	Division	NNP
cord-283109-ka3n9pft	75	18	of	of	IN
cord-283109-ka3n9pft	75	19	Viral	Viral	NNP
cord-283109-ka3n9pft	75	20	Diseases	Diseases	NNPS