id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-319635-kh99n7q2	Chiang, Wei-Wei	Cell Type-Dependent RNA Recombination Frequency in the Japanese Encephalitis Virus	2014-07-22		.txt	text/plain	5101	281	55	Such cleaved small RNA fragments may be further degraded through an RNA interference pathway triggered by viral double-stranded RNA during replication in mosquito cells, resulting in a lower frequency of RNA recombination in mosquito cells compared to that which occurs in mammalian cells. Yates' chi-square test was used to assess the frequency of RNA recombination in cells coinfected by two virus strains or transfected by viral RNA fragments. Two and one recombinant form(s) were, respectively, identified in selected samples from BHK-21 and C6/36 cells, when they were coinfected with the T1P1-S1 and CJN-S1 strains of the Japanese encephalitis virus. As in our previous report, different strains of the JEV can coinfect host cells derived from mosquitoes or mammals [25] , which actually generates recombinant forms of the virus [30] . In this study, we infected host cells with Nakayama strains of the JEV, followed by transfection of the (+)5 3 -UTR-I RNA fragment.	./cache/cord-319635-kh99n7q2.txt	./txt/cord-319635-kh99n7q2.txt