id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-001572-ap4ro5me	Oosterhoff, Dinja	Hematopoietic Cancer Cell Lines Can Support Replication of Sabin Poliovirus Type 1	2015-02-28		.txt	text/plain	6150	267	52	To determine replication kinetics, the susceptible tumor cell lines were infected with Sabin poliovirus type 1 from the parental virus or virus that was passaged for 5 times on the hematopoietic cell lines at MOI 1 or MOI 0.01, and samples of the supernatant and cellular lysates were harvested at different time points. To determine whether hematopoietic cell lines can support replication of Sabin poliovirus type 1, cells were infected with an MOI of 1 and cells together with supernatant were harvested at day 3 (for all virus passages in Vero cells and for passages 3-5 on U937 cells) or day 6 after infection. In the supernatant of all cell lines tested, at day 4, a high virus titer, comparable to Sabin poliovirus type 1 replicated in Vero cells, was observed in the culture medium, indicating that virus replication was efficient during multiple rounds of replication.	./cache/cord-001572-ap4ro5me.txt	./txt/cord-001572-ap4ro5me.txt