id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-353797-yb355mxk	Inaba, Y.	Replication of bovine coronavirus in cell line BEK-1 culture	1976		.txt	text/plain	835	51	55	Bovine coronavirus readily multiplied and induced marked cytopathic effect in BEK-1 cultures, thus providing a sensitive, practical assay system for viral infectivity and neutralizing antibody and a satisfactory source of the virus. 1V[~iBus and his associates (4--6) have demonstrated an agent with morphologic features of coronavirus by electron microscopy in fractions prepared by density gradient ultracentrifugation from diarrheal feces of calves with natural and experimentally produced neonatal diarrhea. The agent multiplied but failed to induce readily recognizable cytopathic effect in bovine embryonic kidney cell cultures (3) . This paper describes briefly our recent observation that the virus readily replicates and induces a marked cytopathic effect in cultures of a continuous cell line, BEK-1, derived from bovine embryonic kidney (2), thus providing a sensitive, practical assay method and a satisfactory source of the virus. Coverslip cultures of BEK-1 cells inoculated with the virus were examined after 2 or 3 days of incubation at 34 ° C.	./cache/cord-353797-yb355mxk.txt	./txt/cord-353797-yb355mxk.txt