id author title date pages extension mime words sentences flesch summary cache txt cord-335690-66t5fjld Khromykh, A. A. RNA binding properties of core protein of the flavivirus Kunjin 1996 .txt text/plain 5858 250 58 For this study we exploited the use of glutathione-S-transferase (GST)-core fusion proteins attached to an insoluble matrix (Glutathione Sepharose 4B) for rapid RNAprotein binding assays, and extended some of these results by mobility shift assays [17] which were possible because of the relatively small size of the RNA probes, KUN UTR probes bound strongly to fusion proteins incorporating full length or mature C, and to the isolated amino-terminal or carboxy-terminal domains of mature C. In attempts to define which regions of the mature form of C were binding to RNA, three regions enriched in basic amino acids were chosen arbitrarily for All these deleted fusion proteins bound to 3' UTR and 5' core probes on beads as efficiently as C107 (Fig. 3a) . ./cache/cord-335690-66t5fjld.txt ./txt/cord-335690-66t5fjld.txt