id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-315811-wpeac0lk	Hu, Hui	Experimental infection of gnotobiotic pigs with the cell-culture-adapted porcine deltacoronavirus strain OH-FD22	2016-09-12		.txt	text/plain	6500	365	62	The aims of our current study were to investigate i) the pathogenicity of the tissue-culture-grown PDCoV (TC-PDCoV) strain OH-FD22 at passage 5 (P5), P20, and P40 on LLC-PK cells in 14-day-old Gn pigs compared with that of the wild-type parent virus [12] ; ii) Fecal PDCoV shedding, viremia, and pathology tested at post-inoculation day (PID) 1 to 23-24 or PID 28; iii) possible attenuation of TC-PDCoV OH-FD22 P40, the highest passage in cell culture among the passages tested; iv) serum-PDCoV-specific IgG and IgA and virus neutralization (VN) antibody titers and any differences in the titers among OH-FD22 P5, P20, and P40-inoculated pigs; and v) the genetic stability of TC-and Gn-pig-passaged PDCoV OH-FD22 P5, P20, and P40 by analysis of the complete spike (S) and nucleocapsid (N) gene sequences. Regardless of the cell culture passage number of the virus strains used, serum virus neutralization (VN) antibodies were first detected in all of the original and TC-PDCoV OH-FD22-inoculated pigs at PID 7 (64-256), and thereafter, the titers increased gradually and peaked at PID 23/24.	./cache/cord-315811-wpeac0lk.txt	./txt/cord-315811-wpeac0lk.txt