id author title date pages extension mime words sentences flesch summary cache txt cord-298883-uiwg482s Truong, C. Identification of an immunorelevant ORF2 epitope from porcine circovirus type 2 as a serological marker for experimental and natural infection 2001 .txt text/plain 4158 216 51 Post-weaning multisystemic wasting syndrome (PMWS) is a recently identified disease of pigs linked to the emergence of a new porcine circovirus (PCV2). We report here the characterization of immunorelevant linear B-cell epitopes of the Open Reading Frame 2-encoded protein (Orf2) from PCV2 by an enzyme-linked immunosorbent assay (ELISA) using experimental antisera collected from pigs inoculated with a PCV2 isolate. Antibodies to the 117 to 131 epitope (B-133) were detected in 22% and 100% of specific pathogen-free (SPF) pig sera 6 and 11 weeks post inoculation, respectively. Here, we describe the characterization of an Orf2 peptide as an immunorelevant PCV2 specific linear B-cell epitope by ELISA, using sera from pigs experimentally inoculated with a PCV2 isolate, and its potential use as a serological marker to detect PCV2 antibodies following natural infection in swine herds. ./cache/cord-298883-uiwg482s.txt ./txt/cord-298883-uiwg482s.txt