id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-004849-d64hnqkh	Choi, C. S.	Temperature dependent replication of porcine parvovirus isolates	1989		.txt	text/plain	2338	121	48	The replication of four porcine parvovirus isolates, NADL-8, NADL-2, KBSH, and Kresse, in swine testes cells were compared at temperatures of 32, 37, and 39 °C. Similar patterns of cell associated virus were detected from both NADL-8 and NADL-2 infected ceils at the 3 temperatures (Fig. 2 a, b) , whereas marked differences were again observed for Kresse and KBSH-infected cells (Fig. 2 c, d) . Since marked differences in replication of Kresse and KBSH isolates were observed at 37 and 39°C evidenced by both intracellular and extracellular infectious virus and HA antigen, further characterization of viral polypeptide production was attempted. Temperature dependent differences in the number and quantity of viral polypeptides were observed in cells infected with Kresse and KBSH and propagated at either 32, 37 or 39 °C (Fig. 3 A) . To further examine the mechanism of temperature-dependent replication of PPV isolates, the synthesis of viral D N A was evaluated by nucleic acid hybridization of cell extracts (Fig. 4) .	./cache/cord-004849-d64hnqkh.txt	./txt/cord-004849-d64hnqkh.txt