id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-004673-c8qcjve9	Faaberg, K. S.	Membrane association of the C-terminal half of the open reading frame 1a protein of lactate dehydrogenase-elevating virus	1996		.txt	text/plain	4647	220	55	cDNAs encoding ORF 1a protein segments encompassing transmembrane segments 5 to 11 and its amphipathic C-terminal end as well as the N-terminal 80 amino acids of the downstream ORF 1b protein were transcribed and the transcripts in vitro translated in the absence and presence of microsomal membranes. Eisenberg hydrophobic moment analysis of the protein predicted the presence of eleven transmembrane segments in the C-terminal half of the molecule (amino acids 980-1852) that flank the serine protease domain, cDNAs encoding ORF la protein segments encompassing transmembrane segments 5 to 11 and its amphipathic C-terminal end as well as the N-terminal 80 amino acids of the downstream ORF lb protein were transcribed and the transcripts in vitro translated in the absence and presence of microsomal membranes. In the present study we provide strong evidence that the segment of LDV ORF la protein with transmembrane segments 5-11 (see Fig. 1 ) becomes intimately associated with endoplasmic reticulum (ER) membranes during synthesis and that none of its potential N-glycosylation sites becomes glycosylated.	./cache/cord-004673-c8qcjve9.txt	./txt/cord-004673-c8qcjve9.txt