id author title date pages extension mime words sentences flesch summary cache txt cord-012431-l2i5utne Sorokin, D. Y. Microbiological analysis of the population of extremely haloalkaliphilic sulfur-oxidizing bacteria dominating in lab-scale sulfide-removing bioreactors 2008-10-01 .txt text/plain 4937 261 48 title: Microbiological analysis of the population of extremely haloalkaliphilic sulfur-oxidizing bacteria dominating in lab-scale sulfide-removing bioreactors Therefore, the feasibility of sulfide oxidation into elemental sulfur under oxygen limitation was tested at extremely haloalkaline conditions in lab-scale bioreactors using mix sediments from hypersaline soda lakes as inoculum. The microbiological analysis, both culture dependent and independent, of the successfully operating bioreactors revealed a domination of obligately chemolithoautotrophic and extremely haloalkaliphilic sulfur-oxidizing bacteria belonging to the genus Thioalkalivibrio. Molecular analysis of the biomass from two SL-BR and two FBR bioreactors based on 16S rRNA gene DGGE showed low genetic diversity, typical for autotrophic mix cultures with domination of one to two SOB genotypes and some side heterotrophic populations (Fig. 4) . In the natural soda lake sediments, often another genus of low salt-tolerant alkaliphilic SOB (Thioalkalimicrobium) can be found, which is characterized by extremely high rates of sulfide oxidation (Sorokin and Kuenen 2005; Sorokin et al. ./cache/cord-012431-l2i5utne.txt ./txt/cord-012431-l2i5utne.txt