id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-348401-x2q9vyf2	Millet, Jean K.	Middle East respiratory syndrome coronavirus infection is inhibited by griffithsin	2016-07-15		.txt	text/plain	4584	260	56	The MERS-CoV spike protein is a main determinant of virus entry into host cells as it mediates both binding to the DPP4 (dipeptidyl peptidase 4) receptor and fusion of the viral envelope with host cell membrane (Millet and Whittaker, 2014; Raj et al., 2013) . Immunofluorescence assay of MERS-CoV-infected Huh-7, MRC-5, and Vero-81 cells in presence of increasing concentrations of griffithsin. In all conditions, cells were infected with MERS-CoV strain EMC/2012 at an m.o.i. of 10, with griffithsin (1 mg/mL) added or not at different steps during virus entry. Because we have performed our assay using high m.o.i. and a short infection time, these results show the strong inhibitory activity of griffithsin on early steps of the MERS-CoV viral cycle. To better define which stage in the virus life cycle griffithsin acts on, we performed an infection assay using authentic MERS-CoV with griffithsin present at different times during viral entry steps (Fig. 4A) .	./cache/cord-348401-x2q9vyf2.txt	./txt/cord-348401-x2q9vyf2.txt