id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-337645-t6py0oyw	Yin, Jiechao	Cholesterol is important for a post-adsorption step in the entry process of transmissible gastroenteritis virus	2010-10-14		.txt	text/plain	4753	268	50	Though S protein was not solubilized by cold non-ionic detergents, this behavior was unchanged when cholesterol was depleted from viral membrane by methyl-β-cyclodextrin (MβCD) and the protein did not comigrate with cellular DRM marker proteins in flotation analyses. A functional analysis suggests that cholesterol depletion affects a post-adsorption step in the virus entry process that requires membrane rearrangements. As cholesterol depletion from TGE virions results in a reduction of viral infectivity (Ren et al., 2008) , we were interested to find out whether the surface protein S that mediates virus entry is localized in DRMs. For this purpose, we analyzed whether the TGEV S protein is resistant to solubilization by Triton X-100 at 4 • C. This result indicates that the surface protein of TGEV is arranged in the viral envelope in a detergent-resistant way that is not affected by cholesterol depletion.	./cache/cord-337645-t6py0oyw.txt	./txt/cord-337645-t6py0oyw.txt