id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-316624-bqaqhp3m	Sui, Xiuwen	Antiviral effect of diammonium glycyrrhizinate and lithium chloride on cell infection by pseudorabies herpesvirus	2009-10-30		.txt	text/plain	3738	219	58	Virus plaque-reduction assays, PCR and RT-PCR analysis indicated that both drugs inhibited cell infection by PrV. Briefly, FITC-conjugated Annexin V (50 l/well) and propidium iodide, PI (50 l/well) were added the cells infected by drug-treated viruses, or the virus-infected cells treated with LiCl in 24-well plates and incubated at room temperature for 15 min in the dark prior to fluorescence observation. At the maximum non-toxic concentration of the drugs, the inhibition rate of LiCl and DG for PrV infection in cell culture reached 100% (Fig. 4) . The effect of both drugs on PrV-infected cells was analyzed using plaque-reduction assays. As shown in Fig. 9 , the maximum nontoxic concentration of drugs had an inhibitory activity against PrV-induced cell apoptosis. It is possible that the observed anti-apoptotic effect of DG and LiCl during PRV infection is indirect and due to the reduced virus replication caused by the drugs.	./cache/cord-316624-bqaqhp3m.txt	./txt/cord-316624-bqaqhp3m.txt