id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-315524-vgdjpjkj	Chen, Sunrui	Drug screening identifies gemcitabine inhibiting rotavirus through alteration of pyrimidine nucleotide synthesis pathway	2020-05-30		.txt	text/plain	2073	135	40	We identified gemcitabine, a widely used anti-cancer drug, as a potent inhibitor of rotavirus infection. Supplementation of UTP or uridine largely abolished the anti-rotavirus activity of gemcitabine, suggesting its function through inhibition of pyrimidine biosynthesis pathway. Furthermore, immunofluorescent staining of viral capsid protein (VP6) showed significant reduction of the number of infected Caco2 cells by gemcitabine treatment (Fig. 1F, 1G, Supplementary Fig. S2 ). Our previous studies have evaluated the effects of the nucleoside analog ribavirin and mycophenolic acid (MPA), and the antiviral cytokine interferon alpha (IFN-α) on rotavirus in cell culture models. We have previously established modeling of rotavirus infection in intestinal organoids, which allows the study of virus-host interactions and assessment of antiviral drugs (Yin et al., 2015a; Yin et al., 2018a; Yin et al., 2018b; Yin et al., 2016) . Gemcitabine, a widely used anti-cancer drug, has potent antiviral activity against rotavirus infection Gemcitabine exerts its anti-rotavirus effect through inhibiting pyrimidine biosynthesis pathway	./cache/cord-315524-vgdjpjkj.txt	./txt/cord-315524-vgdjpjkj.txt