id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-299189-59d4aojh	Zou, Hao	Transmissible gastroenteritis virus: Identification of M protein-binding peptide ligands with antiviral and diagnostic potential	2013-07-02		.txt	text/plain	4558	262	54	title: Transmissible gastroenteritis virus: Identification of M protein-binding peptide ligands with antiviral and diagnostic potential In this study, the M protein of transmissible gastroenteritis virus (TGEV) was used to biopan a 12-mer phage display random peptide library. Plaque-reduction assays revealed that pepTGEV-M7 was able to prevent TGEV infection in vitro (p < 0.01) following pretreatment of the virus with the peptide. coli (Fig. 1) for use in biopanning the phage display library to identify peptides that bind the M protein of TGEV. Ten different TGEV-M protein-reactive phages were identified by ELISA, when rTGEV-M was used as the coating antigen (Fig. 2a) . 100TCID50 TGEV virus was pre-incubated with 2-fold, serially-diluted (500-31.25 lg/ml) peptide (pepTGEV-M7) then added to ST cells for 48 h. Bacterial expression of antigenic sites A and D in the spike protein of transmissible gastroenteritis virus and evaluation of their inhibitory effects on viral infection	./cache/cord-299189-59d4aojh.txt	./txt/cord-299189-59d4aojh.txt