id author title date pages extension mime words sentences flesch summary cache txt cord-288337-sw7xjpbr Guo, Chao-Tan Edible bird's nest extract inhibits influenza virus infection 2006-03-03 .txt text/plain 4159 195 53 Five microliters of influenza virus suspension (500 ng protein) in 50 mM sodium acetate buffer (pH 5.5) containing two-fold serial dilution of the EBN extracts was stored at 37 • C for 30 min and then incubated with 5 l of 4 mM 2 -(4-methylumbelliferyl)-␣-d-N-acetylneuraminic acid (4-MU-Neu5Ac) (Sigma-Aldrich, St. Louis, MO) at 37 • C for 30 min. In order to check the reaction of EBN extract with influenza viruses, we used the human influenza virus strain A/Aichi/2/68 (H3N2), which bound to both sialyl ␣2-3 and ␣2-6 galactose linkages in salylglycoproteins and sialylglycolipids, and carried out an SDS-PAGE Western blotting assay to analyze the glycoprotein molecules. As shown in Fig. 2A , in case of no treatment with the pancreatic enzyme, neither the EBN extract from S1 nor that from S2 inhibited the hemagglutination of influenza A virus (strain A/Aichi/2/68) to human type O erythrocytes. ./cache/cord-288337-sw7xjpbr.txt ./txt/cord-288337-sw7xjpbr.txt