id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-284076-087oltss	Patel, Deendayal	Peptide-conjugated morpholino oligomers inhibit porcine reproductive and respiratory syndrome virus replication	2007-10-04		.txt	text/plain	7761	411	56	Of 12 peptide-conjugated PMO (PPMO), four were found to be highly effective at inhibiting PRRSV replication in cell culture in a dose-dependant and sequence-specific manner. In a previous study (Zhang et al., 2006) , we tested six PPMO and found one of them (5UP1), designed to target the 5 terminal region of the PRRSV genome, to be a highly effective inhibitor of PRRSV replication in a sequence-specific and dose-dependent manner. Confirmation of the CPE observations and PRRSV yield titration was obtained by IFA on CRL11171 cells after virus inoculation and PPMO treatment (Fig. 2B ). demonstrated that PPMO generated little cytotoxicity in both CRL11171 and PAM cells, further indicating that the suppression of virus replication observed in the antiviral experiments above was due to sequence-specific effects. Treatment of cells with PPMO 5UP2 or 5HP led to suppression of PRRSV replication of all North American strains, producing virus yields not detectable (ND) in this assay.	./cache/cord-284076-087oltss.txt	./txt/cord-284076-087oltss.txt