id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-281385-oxohdfpu	Noble, Christian G.	Crystal structure of dengue virus methyltransferase without S-adenosyl-L-methionine	2014-09-19		.txt	text/plain	2621	152	58	Crystal structures of almost all available flavivirus methyltransferases contain S-adenosyl-L-methionine (SAM), the methyl donor molecule that co-purifies with the enzymes. The SAM-depleted and SAM-containing MTases exhibited comparable enzymatic activities (N-7 MTase, 2 0 -O MTase, and covalent GMP-MTase complex formation the natural product Sinefungin, showing that this is a viable approach to identify novel small molecules that bind to the same pocket. This conclusion was supported by two complementary structural and functional approaches: (i) the crystal structure unequivocally shows that absence of SAM does not affect the overall conformation of DENV MTase, including the SAM-binding pocket; (ii) depletion of SAM from the recombinant MTase did not change the activities of cap methylations and GMP-MTase complex formation. Structural and functional analysis of methylation and 5 0 -RNA sequence requirements of short capped RNAs by the methyltransferase domain of dengue virus NS5	./cache/cord-281385-oxohdfpu.txt	./txt/cord-281385-oxohdfpu.txt