id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-264488-989t9ld1	Park, Il-Hyun	Inhibition of hepatitis B virus replication by ligand-mediated activation of RNase L	2014-02-06		.txt	text/plain	5320	269	54	In the present study, the potential antiviral activity of RNase L against hepatitis B virus (HBV) was explored utilizing the recently reported infection protocol based on human hepatoma HepG2 cells stably complemented with the virus entry factor NTCP. These results suggest that HBV replication can be regulated through interferon-mediated RNA decay pathways and that activation of these host antiviral factors may represent a novel therapeutic strategy for HBV infection. Our data indicate that ligand-mediated activation of these enzymes results in a marked inhibition of HBV replication in both infected and transfected cells. With the newly established HepG2-NTCP cell culture system, which permits HBV infection, we showed that viral gene expression and replication can be profoundly reduced by 2-5Aor poly(I:C)-mediated activation of RNase L. In HBV1.2-transfected Huh-7 cells, it was further confirmed that this type of inhibition occurred mostly through viral RNA degradation via the ribonuclease function of RNase L.	./cache/cord-264488-989t9ld1.txt	./txt/cord-264488-989t9ld1.txt