id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-262184-uxyb4vih	Jockusch, Steffen	A Library of Nucleotide Analogues Terminate RNA Synthesis Catalyzed by Polymerases of Coronaviruses that Cause SARS and COVID-19	2020-06-18		.txt	text/plain	6488	395	54	We previously demonstrated that five nucleotide analogues inhibit the SARS-CoV-2 RNA-dependent RNA polymerase (RdRp), including the active triphosphate forms of Sofosbuvir, Alovudine, Zidovudine, Tenofovir alafenamide and Emtricitabine. Using the criteria above, our study examines 11 nucleotide analogues with sugar or base modifications (structures shown in Fig. 1 ) for their ability to inhibit the SARS-CoV-2 or SARS-CoV RdRps: Ganciclovir 5'-triphosphate, Carbovir 5'-triphosphate, Cidofovir diphosphate, Stavudine 5'-triphosphate, Entecavir 5'-triphosphate, 2'-O-methyluridine-5'-triphosphate (2'-OMe-UTP), 3'-O-methyluridine-5'triphosphate (3'-OMe-UTP), 2'-fluoro-2'-deoxyuridine-5'-triphosphate (2'-F-dUTP), desthiobiotin-16aminoallyl-uridine-5'-triphosphate (Desthiobiotin-16-UTP), biotin-16-aminoallyl-2'-deoxyuridine-5'triphosphate (Biotin-16-dUTP) and 2'-amino-2'-deoxyuridine-5'-triphosphate (2'-NH 2 -dUTP). We then performed polymerase extension assays with the library of nucleoside triphosphate analogues (Fig. 1) either alone or in combination with natural nucleotides: 2'-OMe-UTP, 3'-OMe-UTP, 2'-F-dUTP, 2'-NH 2 -dUTP, Biotin-UTP, desthiobiotin-16-UTP, Sta-TP, Cid-DP + UTP + ATP, Car-TP + UTP + ATP + CTP, Gan-TP + UTP + ATP + CTP, or Ent-TP + UTP + ATP + CTP, following the addition of a pre-annealed RNA template and primer to a pre-assembled mixture of the SARS-CoV and/or SARS-CoV-2 RdRp (nsp12) and the two cofactor proteins (nsp7 and nsp8).	./cache/cord-262184-uxyb4vih.txt	./txt/cord-262184-uxyb4vih.txt