id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-262110-569a86u3	Tan, Chee Wah	Inhibition of enterovirus 71 infection by antisense octaguanidinium dendrimer-conjugated morpholino oligomers	2014-04-24		.txt	text/plain	4223	253	54	Octaguanidinium-conjugated morpholino oligomers (vivo-MOs) are single-stranded DNA-like antisense agents that can readily penetrate cells and reduce gene expression by steric blocking of complementary RNA sequences. In this study, three octaguanidinium dendrimer conjugatedmorpholino oligomers (vivo-MOs) targeting the EV-71 internal ribosome entry site (IRES) core sequence and the RNA-dependent RNA polymerase (RdRP) were tested for their inhibitory effects against EV-71. After incubation, the inoculum was removed and replenished with maintenance medium (DMEM with 2% FBS) with or without vivo-MOs. The inhibitory effects of the vivo-MOs were evaluated by plaque assay, TaqMan real-time RT-PCR and western blot analysis 24 h post-infection (hpi) as previously described (Tan et al., 2012 . As shown in Fig. 2 , both vivo-MOs targeting the EV-71 IRES stemloop region exhibited significant antiviral activity against EV-71 infection with reduction of virus-induced CPE ( Fig. 2A) , plaque formation (Fig. 2B) , RNA (Fig. 2C ) and capsid expression (Fig. 2D ) in a dose-dependent manner.	./cache/cord-262110-569a86u3.txt	./txt/cord-262110-569a86u3.txt